| Contributors |
| Preface |
| Volume in Series |
| Inflammation, Angiogenesis, and Lymphangiogenesis / Cornelia Halin ; Michael Detmar1： |
| Introduction |
| Angiogenesis and Lymphangiogenesis in Inflammation / 2： |
| Mouse Models of Inflammation / 3： |
| Commonly Used Markers of Blood Vessels and of Lymphatic Vessels / 4： |
| Measuring the (Lymph)angiogenic Response by Immunofluorescence / 5： |
| Quantitating (Lymph)angiogenesis by Fluorescence-Activated Cell Sorting / 6： |
| Measuring Vascular Permeability and Flow / 7： |
| Material and Methods / 8： |
| Acknowledgments |
| References |
| Color-Coded Fluorescent Mouse Models of Cancer Cell Interactions with Blood Vessels and Lymphatics / Michele McElroy ; Michael Bouvet ; Robert M. Hoffman |
| Mouse Models: Fluorescent Tumors to Image Angiogenesis |
| Color-Coded Tumor-Host Models: GFP Nude Mouse |
| Color-Coded Tumor-Host Models: Nestin-Driven-GFP Nude Mouse |
| Imaging Cancer Cell Trafficking in Lymphatics: Experimental Metastasis Model |
| Imaging Cancer Cell Trafficking in Lymphatics: Spontaneous Metastasis Model |
| Protocol: Production of Fluorescent-Protein-Expressing Cancer Cell Lines |
| Protocol: Imaging of Cancer Cell Interactions with Blood Vessels and Lymphatics |
| Protocol: Imaging Methods for Mice Expressing Fluorescent Proteins / 9： |
| Summary and Conclusions / 10： |
| Bone Marrow-Derived Vascular Progenitors and Proangiogenic Monocytes in Tumors / Kan Lu ; Chrystelle Lamagna ; Gabriele Bergers |
| Methods for the Visualization of BMDCs in Tumors |
| Analysis of Pericyte Progenitors in Tumors |
| Screening Phage-Display Peptide Libraries for Vascular Targeted Peptides / Martin Trepel ; Renata Pasqualini ; Wadih Arap |
| Methods |
| Concluding Remarks and Perspective |
| Avian Embryos: A Model for the Study of Primary Vascular Assembly in Warm-Blooded Animals / Paul A. Rupp ; Mike B. Filla ; Cheng Cui ; Charles D. Little |
| Culture Insert Preparation |
| Culture Chamber Preparation |
| Construction of Microscope Incubator |
| Construction of Electroporation Chamber |
| Embryonic Culture Insert/Dish Preparation |
| Cell Labeling |
| Post-Incubation Fixation and Processing |
| Whole-Mount Immunolabeling |
| Plastic Embedding and Sectioning |
| Mouse Models to Investigate Anti-Cancer Effects of VEGF Inhibitors / Yongping Crawford ; Napoleone Ferrara |
| Xenografts |
| Genetic Models |
| Conclusions |
| Molecular Imaging of Tumor Vasculature / Weibo Cai ; Sanjiv S. Gambhir ; Xiaoyuan Chen |
| Structural/Functional Imaging of Tumor Vasculature |
| Molecular Imaging |
| Imaging Integrin [alpha subscript v beta subscript 3] in Tumor Vasculature |
| Non-Radionuclide-Based Imaging of VEGFR in Tumor Vasculature |
| Radionuclide-Based Imaging of VEGFR in Tumor Vasculature |
| Experimental Section |
| Summary |
| Proteomic Mapping of the Vascular Endothelium In Vivo for Vascular Targeting / Noelle M. Griffin ; Jan E. Schnitzer |
| Proteomics: An Overview |
| Proteomic Methodologies |
| Databases |
| Quantitative Proteomics |
| Application of Proteomics to the Vasculature |
| Protocols |
| Development of Coronary Vessels / Xiu Rong Dong ; Colin T. Maguire ; San-Pin Wu ; Mark W. Majesky |
| Microdissection and Explant Culture of the Proepicardium |
| Isolation of Total RNA from Individual PEs for Gene Expression Studies |
| Explant Culture of the Epicardium |
| Analysis of PE and Epicardium In Vivo by Scanning Electron Microscopy |
| Methods to Study Coronary Vessel Development In Vivo |
| Methods for Evaluating Uteroplacental Angiogenesis and Their Application Using Animal Models / Pawel P. Borowicz ; Shireen Hafez ; Dale A. Redmer ; Lawrence P. Reynolds |
| Importance of the Uteroplacental Circulation to Normal Fetal Growth and Development |
| Methods to Evaluate and Quantify Placental Angiogenesis |
| What we have Learned from Studies of Uteroplacental Angiogenesis Using Animal Models |
| Intravital Microscopic Investigation of Leukocyte Interactions with the Blood Vessel Wall / Klaus Ley ; Javier Mestas ; Maria K. Pospieszalska ; Prithu Sundd ; Alexander Groisman ; Alexander Zarbock11： |
| Transillumination Intravital Microscopy |
| Measuring Leukocyte Rolling, Adhesion, and Transmigration |
| Spinning-Disk Confocal and Two-Photon Microscopy |
| Conclusion |
| Placental Remodeling of the Uterine Vasculature / Nathan M. Hunkapiller ; Susan J. Fisher12： |
| Isolation and Culture of Human Cytotrophoblasts |
| Isolation and Culture of First-Trimester, Human Placental Villous Explants |
| Identification of Cytotrophoblast-Modified Blood Vessels in Tissue Sections |
| Cytotrophoblast Migration and Induction of Endothelial Cell Apoptosis During Co-Culture |
| In Vitro Models of CTB Endovascular Invasion Using Explanted Spiral Arterioles |
| In Vivo Models of Human Cytotrophoblast Vascular Remodeling |
| An In Vivo Experimental Model for Postnatal Vasculogenesis / Juan M. Melero-Martin ; Joyce Bischoff13： |
| Isolation of Blood-Derived Endothelial Progenitor Cells |
| Expansion and Characterization of Blood-derived Endothelial Progenitor Cells |
| Growth of Human Smooth Muscle Cells |
| In Vivo Vasculogenic Assay |
| Assessment of Arteriogenesis / Michael Simons14： |
| Anatomical Assessment of Arteriogenesis |
| Microangiography |
| Functional Assessment of Arteriogenesis |
| Laser-Doppler Perfusion Imaging |
| Electron Paramagnetic Resonance Tissue Oxymetry |
| Magnetic Resonance Imaging |
| Methods to Study Myeloid Cell Roles in Angiogenesis / Michael C. Schmid ; Judith A. Varner15： |
| Methods for the Study of Myeloid Cells in Tumor Neovascularization and Growth |
| Author Index |
| Subject Index |
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