| Expression, Purification, and Posttranslational Modification / A.J. Self ; A. Hall |
| Purification of Recombinant Rho/Rac/G25K fromEscherichia coli / R.A.Cerione ; D. Leonard ; Y. Zheng |
| Purification of Baculovirus-Expressed Cdc42Hs / T. Mizuno ; H. Nakanishi ; Y. Takai |
| Purification and Properties of Rac2 from Human Leukemia Cells / U.G. Knaus ; G.M. Bokoch |
| Purification of Rac2 from Human Neutrophils / A. Abo |
| Purification of Rac(GDI Dissociation Inhibitor Complex from Phagocyte Cytosol / K. Tanaka ; T. Sasaki |
| Purification and Properties of Recombinant Rho(GDP Dissociation Inhibitor / M.R. Philips ; M.H. Pillinger |
| Prenylcysteine-Directed Carboxyl Methyltransferase Activity in Human Neutrophil Membranes |
| Guanine Nucleotide Exchange and Hydrolysis |
| Measurement of Intrinsic Nucleotide Exchange and GTP Hydrolysis Rates / M.J. Hart ; R.A. Cerione |
| Guanine Nucleotide Exchange Catalyzed by dbl Oncogene Product / E. Porfiri ; J.F. Hancock |
| Stimulation of Nucleotide Exchange on Ras- and Rho-Related Proteins by Small GTP-Binding Protein GDP Dissociation Stimulator / T. Miki |
| Interaction of Ect2 and Dblwith Rho-related GTPases / D.A. Leonard |
| Solubilization of Cdc42Hs from Membranes by Rho(GDP Dissociation Inhibitor / J. Settleman ; R. Foster |
| Purification and GTPase-Activating Protein Activity of Baculovirus Expressed p190 / S. Ahmed ; R. Kozma ; C. Hall ; L. Lim |
| GTPase-Activating Protein Activity of n(alpha1)-Chimaerin and Effect of Lipids / D.E.H. Afar ; O.N. Witte |
| Characterization of Breakdown Cluster Region Kinase and SH2-Binding Activities / E. Manser ; T. Leung |
| Identification of GTPase-Activating Proteins by Nitrocellulose Overlay Assay / P. Cicchetti ; D. Baltimore |
| Identification of 3BP-1 in cDNA Expression Library by SH3 Domain Screening |
| Cell Expression and in Vitro Analysis / P. Fort ; S. Vincent |
| Serum Induction of RhoG Expression / H. Paterson ; P. Adamson ; D. Robertson |
| Microinjection of Epitope-Tagged Rho Family cDNAs and Analysis by Immunolabeling / S.T. Dillon ; L.A.Feig |
| Purification and Assay of Recombinant C3 Transferase / K. Aktories ; I. Just |
| In Vitro ADP-Ribosylation of Rho by Bacterial ADP-Ribosyltransferases / N. Morii ; S. Narumiya |
| Preparation of Native and Recombinant Clostridium botulinum C3 ADP-Ribosyltransferase and Identification of Rho Proteins by ADP-Ribosylation / D. Diekmann |
| In Vitro Binding Assay for Interactions of Rho and Rac with GTPase-Activating Proteins and Effectors |
| Purification and Assay of Kinases That Interact with Rac/Cdc42 / P. Aspenstrim ; M.F. Olson |
| Yeast Two-Hybrid System to Detect Protein(Protein Interactions with Rho GTPases / S.E. Rittenhouse |
| Assay for Rho-Dependent Phosphoinositide 3-Kinase Activity in Platelet Cytosol / E.P. Bowman ; D.J. Uhlinger ; J.D. Lambeth |
| Neutrophil Phospholipase D: Inhibition by Rho(GDP Dissociation Inhibitor and Stimulation by Small GTPase GDP Dissociation Stimulator / M.T. Quinn |
| Measurement of Rac Translocation from Cytosol to Membranes in Activated Neutrophils / A.W. Segal |
| Reconst |
| Overview: The Na,K-Pump. Preparation of Na+,K+-ATPase and Subunits / J.C. Skou |
| Purification of Na+,K+-ATPase: Enzyme Sources, Preparative Problems, and Preparation from Mammalian Kidney / P.L. Jorgensen |
| Preparation of Membrane Na+,K+-ATPase from Rectal Glands of Squalus acanthias / M. Esmann |
| Purification of Na+,K+-ATPase from the Supraorbital Salt Gland of the Duck / T.W. Smith |
| Preparation of Na+,K+-ATPase from Brine Shrimp / G.L. Peterson ; L.E. Hokin |
| Preparation of the *ga(+) Isozyme of the Na+,K+-ATPase from Mammalian Axolemma / K.J. Sweadner |
| Solubilization of Na+,K+-ATPase |
| Crystallization of Membrane-Bound Na+,K+-ATPase in Two Dimensions / E. Skriver ; A.B. Maunsbach ; H. Hebert |
| Preparation of Antibodies to Na+,K+-ATPase and Its Subunits / W.J. Ball, Jr. ; T.L. Kirley ; L.K. Lane |
| Assay of Na+,K+-ATPase Activity |
| ATPase and Phosphatase Activity of Na+,K+-ATPase: Molar and Specific Activity, Protein Determination |
| Coupled Assay of Na+,K+-ATPase Activity / J.G. Norby |
| Identification and Quantitation of Na+,K+-ATPase by Back-Door Phosphorylation / M.D. Resh |
| Reconstitution of Na,K-Pump Activity |
| Reconstitution of Na,K-Pump Activity by Cholate Dialysis: Sidedness and Stoichiometry / S.M. Goldin ; M. Forgac ; G. Chin |
| Reconstitution of the Na,K-Pump by Freeze*b1Thaw Sonication: Estimation of Coupling Ratio and Electrogenicity / J.F. Dixon |
| Incorporation of C12E8-Solubilized Na+,K+-ATPase into Liposomes: Determination of Sidedness and Orientation / F. Cornelius |
| Analysis of the Pump Cycle |
| Measurement of Na+ and K+ Transport and Na+,K+-ATPase Activity in Inside-Out Vesicles from Mammalian Erythrocytes / R. Blostein |
| Measurement of Active and Passive Na+ and K+ Fluxes in Reconstituted Vesicles / S.J.D. Karlish |
| Measurement of Ligand Binding and Distance between Ligands |
| Measurement of Binding of ATP and ADP to Na+,K+-ATPase / J. Jensen |
| Interaction of Cardiac Glycosides with Na+,K+-ATPase / E.T. Wallick ; A. Schwartz |
| Estimation of Na,K-Pump Numbers and Turnover in Intact Cells with [3H]Ouabain / S.R. Hootman ; S.A. Ernst |
| Measurement of Binding of Na+, K+, and Rb+ to Na+,K+-ATPase by Centrifugation Methods / H. Matsui ; H. Homareda |
| Estimating Affinities for Physiological Ligands and Inhibitors by Kinetic Studies on Na+,K+-ATPase and Its Partial Activities / J.D. Robinson |
| Inhibition of Translocation Reactions by Vanadate / L. Beaugi |
| Measurements of Conformational States of Na+,K+-ATPase |
| Use of Formycin Nucleotides, Intrinsic Protein Fluorescence, and Fluorescein Isothiocyanate-Labeled Enzymes for Measurement of Conformational States of Na+,K+-ATPase |
| Eosin as a Fluorescence Probe for Measurement of Conformational States of Na+,K+-ATPase |
| Rapid Ion-Exchange Technique for Measuring Rates of Release of Occluded Ions / I.M. Glynn ; D.E. Richards |
| Modification of Na+,K+-ATPase |
| Proteolytic Cleavage as a Tool for Studying Structure and Conformation of Pure Membrane-Bound Na+,K+-ATPase / R.A. Farley |
| Irreversible and Reversible Modification of SH Groups and Effect on Catalytic Activity / W. Schoner ; M. Hasselberg ; R. Kison |
| Photoaffinity Labeling with ATP Analogs / G. Scheiner-Bobis |
| Affinity Labeling of the Digitalis-Binding Site / B. Rossi ; M. Lazdunski |
| Determination of Quaternary Structure of an Active Enzyme Using Chemical Cross-Linking with Glutaraldehyde / W.S. Craig |
| Use of Cross-Linking Reagents for Detection of Subunit Interactions of Membrane-Bound Na+,K+-ATPase / W.-h. Huang ; S.S. Kakar ; S.M. Periyasamy ; A. Askari |
| Magnetic Resonance Studies of Na+,K+-ATPase |
| Nuclear Magnetic Resonance Investigations of Na+,K+-ATPase / C.M. Grisham |
| Electron Spin Resonance Investigations of Na+,K+-ATPase. Biogenesis and Membrane Assembly |
| Molecular Cloning of Na+,K+-ATPase *ga Subunit Gene Usin / J.W. Schneider ; R.W. Mercer ; E.J. Benz, Jr. ; R. Levenson |
| Basal Transcription and Regulation of Transcription: Transcription Factors, Activators, and Repressors / H.E. Choy |
| A Complete Ribosome-Free in Vitro Transcription System Responsive to Extract of Escherichiacoli / A. Miller ; M. Choi ; M.A. Glucksmann-Kuis ; X. Dai ; L.B. Rothman-Denes |
| Single-Stranded DNA-Binding Proteins as Transcriptional Activators / S.E. Hasnain ; S. Habib ; A. Jain ; S. Burma ; B. Mukherjee |
| Host Factor with Single-Stranded DNA-Binding Activity Involved in Transcription from Baculovirus Polyhedrin Promoter / N. Goosen ; P. van Ulsen ; L. Zulianello ; P. van de Putte |
| Transcription Activiation by Histone-like Protein IHF / D.M. Hinton ; R. March-Amegadzie ; J.S. Gerber ; M. Sharma |
| Bacteriophage T4 Middle Transcription System: T4-Modified RNA Polymerase, AsiA (Sigma-70 Binding Protein), and Transcriptional Activator MotA / H. Ge ; E. Martinez ; C.-M. Chiang ; R.G. Roeder |
| Activator-Dependent Transcription by Mammalian RNA Polymerase II: In Vitro Reconstitution with General Transcription Factors and Cofactors / E. Maldonado ; R. Drapkin ; D. Reinberg |
| Purification of Human RNA Polymerase II and General Transcription Factors / W.-C. Yeh ; J. Hou ; S.L. McKnight |
| Purification and Characterization of Gene-Specific Transcription Factors from Rat Liver: c/EBP, GABP, and IL-4 Stat / M. Zhong ; J. Wisniewski ; M. Fritsch ; M. Mizuguchi ; A. Orosz ; P. Jedlicka ; C. Wu |
| Purification of Heat ShockTranscription Factor of Drosophila / J. Greenblatt ; C.J. Ingles |
| Interaction between Acidic Transcriptional Activation Domains of Activator Protein VP16 Herpes Simplex Virus and TRIID / M. Carey ; D.Tantin ; T. Chi ; R. Hori ; S. Pyo |
| Biochemical Mechanism of Transcriptional Activation by Ga14-VP16 / R. Valcarcel ; H.G. Stunnenberg |
| Retinoid-Dependent in Vitro Transcription / D. Thanos ; T. Maniatis |
| In Vitro Assembly of Enhancer Complexes / G. Stegar ; J. Ham ; M. Yaniv |
| E2 Proteins: Modulators of Papillomavirus Transcription and Replication |
| Chromatin as Templates for Transcription / L.P. ONeill ; B.M. Turner |
| Immunoprecipitation of Chromatin / F. Thoma |
| Mapping of Nucleosome Positions / M.P.Kladde ; R.T. Simpson |
| Chromatin Structure Mapping in Vivo Using Methyltransferases / A.E. Sippel ; H. Saueressig ; M.C. Huber ; H.C. Hoefer ; A. Stief ; U. Borgmeyer ; C. Bonifer |
| Identification of cis-Acting Elements as DNase I Hypersensitive Sites in Lysozyme Gene Chromatin / V.M. Studitsky ; D.J. Clarke ; G. Felsenfeld |
| Preparation of Nucleosomal Templates for Transcription in Vitro / K. Ura ; A.P. Wolffe |
| Reconstruction of Transcriptionally Active and Silent Chromatin / C. Ueguchi |
| Purification of H-NS Protein and Its Regulatory Effect on Transcription in Vitro / R.T. Utley ; T.A.O. Hughes ; L.-J. Juan ; J. Cite ; C.C. Adams ; J.L. Workman |
| In Vitro Analysis of Transcription Factor Binding to Nucleosomes and Nucleosome Disruption/Displacement / T. Tsukiyama |
| Purification of GAGA Factor of Drosophila and Its Role in Nucleosome Disruption / M.C. Barton ; B.M. Emerson |
| Regulated Gene Expression in Reconstituted Chromatin and Synthetic Nuclei of Xenopus |
| Transcription Elongation and Termination: Factors and Complexes / S. Borukhov ; A. Goldfarb |
| Purification and Assay of Escherichia coli Transcript Cleavage Factors GreA and GreB / M. Kashlev ; E. Nudler ; K.Severinov, S. |
| Contributors to Volume 304 |
| Preface |
| Volumes in Series |
| Assembly of Nucleosomes, Chromatin, and Nuclei / Section I.： |
| Preparation of Nucleosome Core Particle from Recombinant Histones / Karolin Luger ; Thomas J. Rechsteiner ; Timothy J. Richmond1.： |
| Assembly of Defined Nucleosomal and Chromatin Arrays from Pure Components / Lenny M. Carruthers ; Christin Tse ; Kerfoot P. Walker III. ; Jeffrey C. Hansen2.： |
| Isolation of Minichromosomes from Yeast Cells / Jennifer A. Alfieri ; David J. Clark3.： |
| Chromatin Assembly in Xenopus Extracts / David John Tremethick4.： |
| In Vitro Reconstitution of Nuclei for Replication and Transcription / Alison J. Crowe ; Michelle Craig Barton5.： |
| Preparation/Analysis of Chromatin Replicated in Vivo and in Isolated Nuclei / Louise Chang ; Colleen A. Ryan ; Carolyn A. Schneider ; Anthony T. Annunziato6.： |
| Interactions of High Mobility Group Box Proteins with DNA and Chromatin / Mair E. A. Churchill ; Anita Changela ; Linda K. Dow ; Adam J. Krieg7.： |
| Reconstitution of High Mobility Group 14/17 Proteins into Nucleosomes and Chromatin / Yuri V. Postnikov ; Michael Bustin8.： |
| Purification and Assays for High Mobility Group HMG-I(Y) Protein Function / Raymond Reeves ; Mark S. Nissen9.： |
| Assays for Nucleosome Structure and Function in Vitro / Section II.： |
| Cryoelectron Microscopic Analysis of Nucleosomes and Chromatin / Jan Bednar ; Christopher L. Woodcock10.： |
| Electron Microscopy of DNA-Protein Compleves and Chromatin / Jack Griffith ; Susan Michalowski ; Alexander M. Makhov11.： |
| Targeted Cross-Linking and DNA Cleavage within Model Chromatin Complexes / Kyu-Min Lee ; David R. Chafin ; Jeffrey J. Hayes12.： |
| Base-Pair Resolution Mapping of Nucleosome Positions Using Site-Directed Hydroxy Radicals / Andrew Flaus13.： |
| Localization of Specific Histone Regions on Nucleosomal DNA by Cross-Linking / Sergei I. Usachenko ; E. Morton Bradbury14.： |
| Restriction Enzymes as Probes of Nucleosome Stability and Dynamics / K. J. Polach ; J. Widom15.： |
| Nucleoprotein Gel Assays for Nucleosome Positioning and Mobility / Sari Pennings16.： |
| Assays for Interaction of Transcription Factor with Nucleosome / Qiao Li ; Ulla Bjork ; Orjan Wrange17.： |
| Assays for Chromatin Remodeling during DNA Repair / Jonathan G. Moggs ; Genevieve Almouzni18.： |
| Nuclear Run-On Assays: Assessing Transcription by Measuring Density of Engaged RNA Polymerases / Kazunori Hirayoshi ; John T. Lis19.： |
| Assays for Chromatin Structure and Function in Vivo / Section III.： |
| Mapping Chromatin Structure in Yeast / Philip D. Gregory ; Wolfram Horz20.： |
| Assays for Nucleosome Positioning in Yeast / Michael P. Ryan ; Grace A. Stafford ; Liuning Yu ; Kellie B. Cummings ; Randall H. Morse21.： |
| Mapping DNA Interaction Sites of Chromosomal Proteins Using Immunoprecipitation and Polymerase Chain Reaction / Andreas Hecht ; Michael Grunstein22.： |
| Immunological Analysis of Yeast Chromatin / Pamela B. Meluh ; James R. Broach23.： |
| DNA Methyltransferases as Probes of Chromatin Structure in Vivo / Michael P. Kladde ; M. Xu ; Robert T. Simpson24.： |
| Mapping Cyclobutane-Pyrimidine Dimers in DNA and Using DNA-Repair by Photolyase for Chromatin Analysis in Yeast / Bernhard Suter ; Magdalena Livingstone-Zatchej ; Fritz Thoma25.： |
| Analysis of Drosophila Chromatin Structure in Vivo / Iain L. Cartwright ; Diane E. Cryderman ; David S. Gilmour ; Lori A. Pile ; Lori L. Wallrath ; Janet A. Weber ; Sarah C. R. Elgin26.： |
| Ultraviolet Cross-Linking Assay to Measure Sequence-Specific DNA Binding in Vivo / Mark D. Biggin27.： |
| DNA-Protein Cross-Linking Applications for Chromatin Studies in Vitro and in Vivo / Dmitry Pruss ; Igor M. Gavin ; Svetlana Melnik ; Sergei G. Bavykin28.： |
| Chromatin Immunoprecipitation Assays in Acetylation Mapping of Higher Eukaryotes / Colyn Crane-Robinson ; Fiona A. Myers ; Tim R. Hebbes ; Alison L. Clayton ; Alan W. Thorne29.： |
| Chromatin Structure Analysis by Ligation-Mediated and Terminal Transferase-Mediated Polymerase Chain Reaction / Gerd P. Pfeifer ; Hsiu-Hua Chen ; Juon-Ichiro Komura ; Arthur D. Riggs30.： |
| Guanine-Adenine Ligation-Mediated Polymerase Chain Reaction in Vivo Footprinting / Erich C. Strauss ; Stuart H. Orkin31.： |
| Exonuclease III as Probe of Chromatin Structure in Vivo / Trevor K. Archer ; Andrea R. Ricci32.： |
| Protein Image Hybridization: Mapping and Positioning DNA-Protein Contacts along DNA / S. Belikov ; O. Preobrazhenskaya ; V. Karpov33.： |
| In Vivo Analysis of Chromatin Structure / Kenneth S. Zaret34.： |
| Analysis of Nucleosome Positioning in Mammalian Cells / Gordon L. Hager ; Gilberto Fragoso35.： |
| Measurement of Localized DNA Supercoiling and Topological Domain Size in Eukaryotic Cells / Phillip R. Kramer ; Olga Bat ; Richard R. Sinden36.： |
| Fluorescent in Situ Hybridization Analysis of Chromosome and Chromatin Structure / Wendy Bickmore37.： |
| Analysis of Nuclear Organization in Saccharomyces cerevisiae / Monica Gotta ; Thierry Laroche ; Susan M. Gasser38.： |
| Chromatin Remodeling Complexes / Section IV.： |
| Histone Acetyltransferases: Preparation of Substrates and Assay Procedures / Craig A. Mizzen ; James E. Brownell ; Richard G. Cook ; C. David Allis39.： |
| Analysis of Activity and Regulation of hGcn5, Human Histone Acetyltransferase / Nickolai A. Barlev ; Jerry L. Workman ; Shelley L. Berger40.： |
| Purification of a Histone Deacetylase Complex from Xenopus laevis: Preparation of Substrates and Assay Procedures / Paul A. Wade ; Peter L. Jones ; Danielle Vermaak ; Alan P. Wolffe41.： |
| Purification and Biochemical Properties of Yeast SWI/SNF Complex / Colin Logie ; Craig L. Peterson42.： |
| Analysis of Modulators of Chromatin Structure in Drosophila / Patrick D. Varga-Weisz ; Edgar J. Bonte ; Peter B. Becker43.： |
| Purification of Drosophila Nucleosome Remodeling Factor / Raphael Sandaltzopoulos ; Vincent Ossipow ; David A. Gdula ; Toshio Tsukiyama ; Carl Wu44.： |
| Author Index |
| Subject Index |
| Protein Folding and Assembly in a Cell-Free Expression System / A.N. Fedorov ; T.O. Baldwin |
| Preparation and Application of Chaperone-Deficient Escherichia coli Cell-free Translation Systems / B.A. Hardesty ; G. Kramer ; T. Zhang ; W. Kudlicki |
| Protein Disulfide Isomerase / H.F. Gilbert ; V. McLean ; M. McLean |
| Thermophilic Fungal Protein Disulfide Isomerase / Y. Yamada ; S. Udaka ; T. Kajino ; C. Miyazaki ; O. Asami ; M. Hirai |
| Disulfide Bond Catalysts in Escherichia coli / J.C.A. Bardwell ; T. Zander |
| Yeast Immunophilins: Purification and Assay of Yeast FKBP12 / J.J. Siekierka ; G. Wiederrecht |
| Peptidylprolyl cis-trans-isomerases from Plant Organelles / A.K. Matoo |
| Purification of GroEL with Low Fluorescence Background / C. Frieden ; A.C. Clark ; R. Ramanathan |
| Overexpression, Purification, and Properties of GroES for Escherichia coli / M. Fisher ; E. Eisenstein ; P. Reddy |
| Criteria for Assessing the Purity and Quality of GroEL / M.J. Todd ; G.H. Lorimer |
| Construction of Single-Ring and Two-Ring Hybrid Versions of Bacterial Chaperonin GroEL / A.L. Horwich ; S.G. Burston ; H.S. Rye ; J.S. Weissman ; W.A. Fenton |
| Chaperonin 6014 and Co-Chaperonin 107 from Chromatium vinosum / B.A. McFadden ; J.A. Torres-Ruiz |
| Chaperonins of Purple Nonsulfur Bacterium Rhodobacter sphaeroides / F.R. Tabita ; W.T. Lee ; G.M.F. Watson |
| Chaperonins from Thermoanaerobacter Species / R.K. Scopes ; K. Truscott |
| Chaperonin from a Thermophile, Thermus thermophilus / M. Yoshida ; H. Taguchi |
| Insect Chaperonin 60: Symbionin / M. Morioka ; H. Ishikawa |
| Purification of Yeast Mitochondrial Chaperonin 60 and Co-Chaperonin 10 / G. Schatz ; Y. Dubaquieacute; ; S. Rospert |
| Purification of Mammalian Mitochondrial Chaperonin 60 through in vitro Reconstitution of Active Oligomers / P.V. Viitanen ; G. Lorimer ; W. Bergmeier ; C. Weiss ; M. Kessel ; P. Goloubinoff |
| Purification of Recombinant Plant and Animal GroES Homologs: Chloroplast and Mitochondrial Chaperonin 10 / K. Bacot ; R. Dickson ; T. Webb |
| Mammalian Cytosolic Chaperonin / N.J. Cowan |
| Electron Microscopy of Chaperonins / H.R. Saibil ; S. Chen ; A.M. Roseman |
| Using Chemical Cross-linking Structural Analysis of GroE Chaperonin Complexes / A. Azem |
| Molecular Chaperones and Their Interactions Investigated by Analytical Ultracentrifugation and Other Methodologies / H-J. Schouml;nfeld ; J. Behlke |
| Probing Conformation of GroEL-Bound Substrate Proteins by Mass Spectrometry / S.E. Radford ; C.V. Robinson ; M. Gross |
| Fluorescence Anisotropy Method for Investigation of GroEL-GroES Interaction / P.M. Horowitz ; B.M. Gorovits |
| Photoincorporation of Fluorescent Probe into GroEL: Defining Site of Interaction / J.W. Seale ; B.T. Brazil |
| Analysis of Chaperone Function Using Citrate Synthase as Nonnative Substrate Protein / J. Buchner ; H. Grallert ; U. Jakob |
| Purification and Characterization of Small Heat Shock Proteins / M. Ehrnsperger ; M. Gaestel ; S. Walke |
| Expression, Purification, and Molecular Chaperone Activity Plant Recombinant Small Heat Shock Proteins / G.J. Lee ; E. Vierling ; J. Horwitz ; Q-L. Huang ; L. Ding |
| Contributor Index |
| Introduction / B.W. Matthews |
| Recent Transformations in Structural Biology |
| Crystals / P.C. Weber |
| Overview of Protein Crystallization Methods / M. Rigs-Kautt ; A. Ducruix |
| Inferences Drawn from Physicochemical Studies of Crystallogenesis and Precrystalline State / L. Song ; J.E. Gouaux |
| Membrane Protein Crystallization: Application of Sparse Matrix to alpha-Hemolysin Heptamer / C.W. Carter, Jr. |
| Response Surface Methods for Optimizing and Improving Reproducibility of Crystal Growth / A. George ; Y. Chiang ; B. Guo ; A. Arabshahi ; Z. Cai ; W.W. Wilson |
| Second Virial Coefficient as Predictor in Protein Crystal Growth / J.R. Luft ; G.T. DeTitta |
| Kinetic Aspects of Macromolecular Crystallization / R. Sousa |
| Using Cosolvents to Stabilize Protein Conformation for Crystallization / C.E. Kundrot |
| Preparation and Crystallization of RNA: A Sparse Matrix Approach / A.R. Ferre-Damare ; S.K. Burley |
| Dynamic Light Scattering in Evaluating Crystallizability of Macromolecules / A.M. Edwards ; S.A. Darst ; S.A. Hemming ; F.J. Asturias ; P.R. David ; R.D. Kornberg |
| Two-Dimensional Protein Crystals in Aid of Three-Dimensional Protein Crystal Growth / I. Rayment |
| Reductive Alkylation of Lysine Residues to Alter Crystallization Properties of Proteins |
| Data: Cryocrystallography / D. Rodgers |
| Practical Cryocrystallography / J.R. Helliwell |
| Overview of Synchrotron Radiation and Macromolecular Crystallography / Z.R. Korszun |
| Neutron Macromolecular Crystallography |
| Detectors / Y. Amemiya |
| X-Ray Storage-Phosphor Imaging-Plate Detectors: High-Sensitivity X-Ray Area Detector / E.M. Westbrook ; I. Naday |
| Charge-Coupled Device-Based Area Detectors / R. Kahn ; R. Fourme |
| Gas Proportional Detectors |
| Data Collection Software / J.W. Pflugrath |
| Diffraction-Data Processing for Electronic Detectors: Theory and Practice / Z. Otwinowski ; W. Minor |
| Processing of X-Ray Diffraction Data Collected in Oscillation Mode / Z. Dauter |
| Data Collection Strategy / T.O. Yeates |
| Detecting and Overcoming Crystal Twinning |
| Phases: Basic Concepts / G. Bricogne |
| Bayesian Statistical Viewpoint on Structure Determination: Basic Concepts and Examples |
| Efficient Sampling Methods for Combinations of Signs |
| Phases, Hyperphases, and Molecular Orientations / H. Ke |
| Overview of Isomorphous Replacement Phasing / M.A. Rould |
| Screening for Heavy-Atom Derivatives and Obtaining Accurate Isomorphous Differences / E. de La Fortelle |
| Maximum-Likelihood Heavy-Atom Parameter Refinement for Multiple Isomorphous Replacement and Multiwavelength Anomalous Diffraction Methods |
| Multiwavelength Methods / W.A. Hendrickson ; C.M. Ogata |
| Phase Determination by Multiwavelength Anomalous Diffraction Methods / S. Doublie |
| Preparation of Selenomethionyl Proteins for Phase Determination / T.C. Terwilliger |
| Multiwavelength Anomalous Diffraction Phasing of Macromolecular Structures: Analysis of MAD Data as Single Isomorphous Replacement with Anomalous Scattering Data Using MADMRG Program / V. Ramakrishnan ; V. Biou |
| Treatment of Multiwavelength Anomalous Diffraction Data as a Special Case of Multiple Isomorphous Replacement |
| Molecular Replacement / A.T. Branger |
| Patterson Correlation Searches and Refinement / J. Navaza ; P. Saludjia |
| Expression, Purification, and Characterization / V. Sardana ; M. Sardana |
| Purification of Viral Polymerases: General Considerations / P.E. Boehmer |
| Expression, Purification, and Characterization of the HerpesSimplex Virus Type-1 DNA Polymerase / D.J. Barton ; B.J. Morasco ; J.B. Flanegan |
| Assays for Poliovirus Polymerase, 3D pol, and Authentic RNA Replication in HeLa S10 Extracts / R. de Francesco ; S.-E. Behrens ; L. Tomei ; S. Altamura ; J. Jiricny |
| RNA-Dependent RNA Polymerase of Hepatitus C Virus / J. Herold ; S. Siddell ; J. Ziebuhr |
| Characterization of Coronavirus RNA Polymerase Gene Products / J. Tomassini |
| Expression, Purification, and Characterization of Orthomyxovirus: Influenza Transcriptase / T. Das ; B.P. De ; A.K. Banerjee |
| Expression, Purification, and Characterization of Rhabdovirus Polymerase / M.W. Stahlhut ; D.B. Olsen |
| Expression and Purification of Retroviral HIV-1 Reverse Transcriptases / L.H.Malkas ; R.J. Hickey |
| Expression, Purification, and Characterization of DNA Polymerases Involved in Papovavirus Replication / M. Ramachandra ; Y. Sasaguri ; R. Nakano ; R. Padmanabhan |
| Heterologous Expression, Purification, and Characterization of Adenovirus DNA Polymerase and Preterminal Protein / J. Hu ; C. Seeger |
| Expression and Characterization of Hepadnavirus Reverse Transcriptases / P. Gershon ; B. Moss |
| Expression, Purification, and Characterization of Vaccinia Virus Encoded RNA and Poly(A)Polymerases / J.R. Sayers |
| Viral Polymerase-Associated 5-3 Exonucleases: Expression, Purification, and Uses |
| Activity Assays: Kinetic and Screening / R.D. Kuchta |
| Isotopic Assays of Viral Polymerases and Related Proteins / J. Eberle ; C. W. Knopf |
| Nonisotopic Assays of Viral Polymerases and Related Proteins / S. Gabbara ; J.A. Peliska |
| Catalytic Activities Associated with Retroviral and Viral Polymerases / J.L. Cole |
| Approaches to High Volume ScreeningAssays of Viral Polymerases and Related Proteins / S.M. Oberhaus ; J.E. Newbold |
| In Situ DNA Polymerase and RNase H Activity Gel Assays as Applied to Hepadnavirus Particles / V. Byrnes ; D. Hazuda |
| A System to Analyze and Identify Inhibitorsof HIV-1 Gene Regulation with Defective Integrated Provirus |
| Design and Analysis of Substrates and Inhibitors / S.S. Carroll ; F. Benseler |
| Preparation and Use of Synthetic Oligoribonucleotides as Tools for Study of Viral Polymerases / V.V. Gurevich |
| Use of Bacteriophage RNA Polymerase in RNA Synthesis / J.E. Wilson ; D.J.T. Porter ; J.E. Reardon |
| Inhibition of Viral Polymerases by Chain Terminating Substrates: A Kinetic Analysis / E.J. Prisbe ; M.S. Chen |
| Design of Nucleoside Analogue Inhibitors of Herpesvirus Polymerases / T.J. Tucker ; W.C. Lumma ; J.C. Culberson |
| Development of Nonnucleoside HIV Reverse Transcriptase Inhibitors / J. Balzarini ; E. De Clerq |
| Analysis of Inhibition of Retroviral Reverse Transcriptase / H. Chen ; D. Brown ; L. Gold |
| Novel Methods of Generating Specific Oligonucleotide Inhibitors of Viral Polymerases |
| Molecular and Structural Characterizations / J.C. Boyer ; K. Bebenek ; T.A. Kunkel |
| AnalyzingFidelity of Transcription and Reverse Transcription / P.L. Boyer ; S.H. Hughes |
| Site-Directed Mutagenic Analysis of Viral Polymerases and Related Proteins / J.S. Oxford ; A.A. Al-Jabri ; P. Levantis |
| Analysis of Resistance Mutants of ViralPolymerases |
| Author Ind |
| Contents of Volumes 228, 230-262 |
| New Photoprotecting Groups: Desyl and p-Hydroxyphenacyl Phosphate and Carboxylate Esters / R.S. Givens ; J.F.W. Weber ; A.H. Jung ; C.-H. Park |
| Synthesis, Photochemistry, and Biological Characterization of Photolabile Protecting Groups for Carboxylic Acids and Neurotransmitters / K.R. Gee ; B.K. Carpenter ; G.P. Hess |
| New Caged Groups: 7-Substituted Coumarinylmethyl Phosphate Esters / T. Furuta ; M. Iwamura |
| Caged Fluorescent Probes / T.J. Mitchison ; K.E. Sawin ; J.A. Theriot ; K. Gee ; A. Mallavarapu |
| Biologically Active Peptides Caged on Tyrosine / R. Sreekumar ; M. Ikebe ; F.S. Fay ; J.W. Walker |
| Light-Directed Activation of Protein Activity from Caged Protein Conjugates / G. Marriott ; J. Ottl ; M. Heidecker ; D. Gabriel |
| Caged Peptides and Proteins by Targeted Chemical Modification / H. Bayley ; C.-Y. Chang ; W.T. Miller ; B. Niblack ; P. Pan |
| Photocleavable Affinity Tags for Isolation and Detection of Biomolecules / J. Olejnik ; E. Krzymantilde;ska-Olejnik ; K.J. Rothschild |
| Synthesis and Applications of Heterobifunctional Photocleavable Cross-Linking Reagents |
| Use of Lasers for One- and Two-Photon Photolysis of Caged Compounds / J.A. McCray |
| Flash Lamp Based Irradiation of Caged Compounds / G. Rapp |
| Fourier Transform Infrared Photolysis Studies of Caged Compounds / V. Cepus ; C. Ulbrich ; C. Allin ; A. Troullier ; K. Gerwert |
| Use of Caged Compounds in Studies of Bioelectronic Imaging and Pattern Recognition / C.H. Wharton ; R.A. Meldrum ; R.S. Chittock |
| Use of Caged Nucleotides to Characterize Unstable Intermediates by X-Ray Crystallography / A. Scheidig ; C. Burmester ; R.S. Goody |
| Photoregulation of Cholinesterase Activities with Caged Cholinergic Ligands / L. Peng ; M. Goeldner |
| Caged Substrates for Measuring Enzymatic Activity in Vivo: Photoactivated Caged Glucose 6-Phosphate / R.R. Swezey ; D. Epel |
| Investigation of Charge Translocation by Ion Pumps and Carriers Using Caged Substrates / K. Fendler ; K. Hartung ; G. Nagel ; E. Bamberg |
| Studies of Molecular Motors Using Caged Compounds / J.A. Dantzig ; H. Higuchi ; Y.E. Goldman |
| Application of Caged Fluorescein-Labeled Tubulin to Studies of Microtubule Dynamics and Transport of Tubulin Molecules in Axons / T. Funakoshi ; N. Hirokawa |
| Two-Photon Activation of Caged Calcium with Submicron, Submillisecond Resolution / E. Brown ; W. Webb |
| Caged Inositol 1,4,5-Trisphosphate for Studying Release of Ca2+ from Intracellular Stores / N. Callamaras ; I. Parker |
| Characterization and Application of Photogeneration of Calcium Mobilizers cADP-Ribose and Nicotinic Acid Adenine Dinucleotide Phosphate from Caged Analogs / K.G. Gee ; H.C. Lee |
| Applications of Caged Compounds of Hydrolysis-Resistant Analogs of cAMP and cGMP / U.B. Kaupp ; C. Dzeja ; S. Frings ; J. Bendig ; V. Hagen |
| Caged Probes for Studying Cellular Physiology: oNitromandelyloxycarbonyl Caging Method Using Caged Glutamate and Ca2ATPase Inhibitor / F.M. Rossi ; M. Margulis ; R.E. Hoesch ; C.-M. Tang ; J.P.Y. Kao |
| Development and Application of Caged Ligands for Neurotransmitter Receptors in Transient Kinetic and Neuronal Circuit Mapping Studies / C. Grewer |
| Caged Plant Growth Regulators / A.C. Allen ; J.L. Ward ; M.H. Beale ; A.J. Trewavas |
| Use of Caged Compounds in S / C.W. Wharton |
| Contributors to Volume 292 |
| Prokaryotic ABC Transporters |
| Overview of Bacterial ABC Transporters / Hiroshi Nikaido ; Jason A. Hall |
| Preparation and Reconstitution of Membrane-Associated Maltose Transporter Complex of Escherichia coli / Amy L. Davidson |
| Maltose Transport in Escherichia coli: Mutations That Uncouple ATP Hydrolysis from Transport / Cynthia H. Panagiotidis ; Howard A. Shuman |
| Binding Protein-Dependent ABC Transport System for Glycerol 3-Phosphate of Escherichia coli / Winfried Boos |
| Structure-Function Analysis of Hemolysin B / Fang Zhang ; Jonathan A. Sheps ; Victor Ling |
| Erwinia Metalloprotease Permease: Aspects of Secretion Pathway and Secretion Functions / Philippe Delepelaire |
| Arsenical Pumps in Prokaryotes and Eukaryotes / Masayuki Kuroda ; Hiranmoy Bhattacharjee ; Barry P. Rosen |
| Eukaryotic ABC Transporters |
| Evolutionary Relationships among ABC Transporters / James M. Croop |
| Cloning of Novel ABC Transporter Genes / Rando Allikmets ; Michael Dean |
| Saccharomyces cerevisiae ABC Proteins and Their Relevance to Human Health and Disease / Daniel Taglicht ; Susan Michaelis |
| Nonmammalian ABC Transport Systems / A.： |
| Arabidopsis MDR Genes: Molecular Cloning and Protein Chemical Aspects / Robert Dudler ; Michael Sidler |
| Functional Analysis of pfmdr1 Gene of Plasmodium falciparum / Sarah Volkman ; Dyann Wirth |
| Amplification of ABC Transporter Gene pgpA and of Other Heavy Metal Resistance Genes in Leishmania tarentolae and Their Study by Gene Transfection and Gene Disruption / Marc Ouellette ; Anass Haimeur ; Katherine Grondin ; Danielle Legare ; Barbara Papadopoulou |
| Functional Assay for Analysis of Yeast ste6 Mutants / Gaby L. Nijbroek |
| ABC Transporters Involved in Transport of Eye Pigment Precursors in Drosophila melanogaster / Gary D. Ewart ; Anthony J. Howells |
| Mammalian P-Glycoproteins / B.： |
| Isolation of Altered-Function Mutants and Genetic Suppressor Elements of Multidrug Transporter P-Glycoprotein by Expression Selection from Retroviral Libraries / Igor B. Roninson ; Donald Zuhn ; Adam Ruth ; David de Graaf |
| Selection and Maintenance of Multidrug-Resistant Cells / Michael M. Gottesman ; Carol Cardarelli ; Sarah Goldenberg ; Thomas Licht ; Ira Pastan |
| Monoclonal Antibodies to P-Glycoprotein: Preparation and Applications to Basic and Clinical Research / Mikihiko Naito ; Takashi Tsuruo |
| Topology of P-Glycoproteins / William R. Skach |
| Use of Cell-Free Systems to Determine P-Glycoprotein Transmembrane Topology / Jian-Ting Zhang |
| Photoaffinity Labels for Characterizing Drug Interaction Sites of P-Glycoprotein / Ahmad R. Safa |
| Identification of Drug Interaction Sites in P-Glycoprotein / Lee M. Greenberger |
| Photoaffinity Labeling of Human P-Glycoprotein: Effect of Modulator Interaction and ATP Hydrolysis on Substrate Binding / Saibal Dey ; Muralidhara ; Ramachandra ; Suresh V. Ambudkar |
| Identification of Phosphorylation Sites in Human MDR1 P-Glycoprotein / Timothy C. Chambers |
| Identification of in Vivo Phosphorylation Sites for Basic-Directed Kinases in Murine mdr1b P-Glycoprotein by Combination of Mass Spectrometry and Site-Directed Mutagenesis / J. S. Glavy ; M. Wolfson ; E. Nieves ; E.-K. Han ; C.-P. H. Yang ; S. B. Horwitz ; G. A. Orr |
| P-Glycoprotein and Swelling-Activated Chloride Channels / Tamara D. Bond ; Christopher F. Higgins ; Miguel A. Valverde |
| Functional Expression of mdr and mdr-like cDNAs in Escherichia coli / Eitan Bibi ; Rotem Edgar ; Oded Beja |
| Yeast Secretory Vesicle System for Expression and Functional Characterization of P-Glycoproteins / Stephan Ruetz |
| High-Level Expression of Mouse Mdr3 P-Glycoprotein in Yeast Pichia pastoris and Characterization of ATPase Activity / Lucille Beaudet ; Ina L. Urbatsch ; Philippe Gros |
| Mutational Analysis of P-Glycoprotein in Yeast Saccharomyces cerevisiae |
| Baculovirus-Mediated Expression of Human Multidrug Resistance cDNA in Insect Cells and Functional Analysis of Recombinant P-Glycoprotein / Ursula A. Germann |
| Recombinant Vaccinia Virus Vectors for Functional Expression of P-Glycoprotein in Mammalian Cells / Muralidhara Ramachandra |
| Functional Expression of Human P-Glycoprotein from Plasmids Using Vaccinia Virus-Bacteriophage T7 RNA Polymerase System / Christine A. Hrycyna |
| pHaMDR-DHFR Bicistronic Expression System for Mutation Analysis of P-Glycoprotein / Shudong Zhang ; Yoshikazu Sugimoto ; Tzipora Shoshani |
| Mutational Analysis of Human P-Glycoprotein / Tip W. Loo ; David M. Clarke |
| Purification and Reconstitution of Human P-Glycoprotein / Isabelle H. Lelong ; Jiaping Zhang |
| Drug-Stimulatable ATPase Activity in Crude Mem-branes of Human MDR1-Transfected Mammalian Cells |
| ATPase Activity of Chinese Hamster P-Glycoprotein / Alan E. Senior ; Marwan K. Al-Shawi |
| Construction of MDR1 Vectors for Gene Therapy |
| Construction of MDR1 Adeno-Associated Virus Vectors for Gene Therapy / Marion Baudard |
| Retroviral Transfer of Multidrug Transporter to Murine Hematopoietic Stem Cells |
| Retroviral Transfer of Human MDR1 Gene into Human T Lymphocytes / Caroline G. L. Lee |
| Construction and Analysis of Multidrug Resistance Transgenic Mice / Gregory L. Evans |
| Multidrug Resistance Associated Protein / C.： |
| Cloning, Transfer, and Characterization of Multi-drug Resistance Protein / Caroline E. Grant ; Gabu Bhardwaj ; Susan P. C. Cole ; Roger G. Deeley |
| Transport Function and Substrate Specificity of Multidrug Resistance Protein / Dietrich Keppler ; Gabriele Jedlitschky ; Inka Leier45.： |
| Cystic Fibrosis Transmembrane Conductance Regulator / D.： |
| Heterologous Expression Systems for Study of Cystic Fibrosis Transmembrane Conductance Regulator / Xiu-Bao Chang ; Norbert Kartner ; Fabian S. Seibert ; Andrei A. Aleksandrov ; Andrew W. Kloser ; Gretchen L. Kiser ; John R. Riordan46.： |
| Characterization of Polyclonal and Monoclonal Antibodies to Cystic Fibrosis Transmembrane Conductance Regulator / 47.： |
| Identification of Cystic Fibrosis Transmembrane Conductance Regulator in Renal Endosomes / Isabelle T. Crawford ; Peter C. Maloney48.： |
| Assays of Dynamics, Mechanisms, and Regulation of ATP Transport and Release: Implications for Study of ABC Transporter Function / Erik M. Schwiebert ; Marie E. Egan ; William B. Guggino49.： |
| Overexpression, Purification, and Function of First Nucleotide-Binding Fold of Cystic Fibrosis Transmembrane Conductance Regulator / Young Hee Ko ; Peter L. Pedersen50.： |
| Recombinant Synthesis of Cystic Fibrosis Trans-membrane Conductance Regulator and Functional Nucleotide-Binding Domains / Scott A. King ; Eric J. Sorscher51.： |
| Cationic Lipid Formulations for Intracellular Gene Delivery of Cystic Fibrosis Transmembrane Conductance Regulator to Airway Epithelia / Seng Hing Cheng ; John Marshall ; Ronald K. Scheule ; Alan E. Smith52.： |
| Adeno-Associated Virus Vectors for Gene Therapy of Cystic Fibrosis / Terence R. Flotte ; Barrie J. Carter53.： |
| Sulfonylurea Receptor / E.： |
| Sulfonylurea Receptors and ATP-Sensitive Potassium Ion Channels / Lydia Aguilar-Bryan ; John P. Clement IV ; Daniel A. Nelson54.： |
| Intracellular ABC Transporters / F.： |
| Peptide Transport Assay for TAP Function / Ye Wang ; David S. Guttoh ; Matthew J. Androlewicz55.： |
| Peroxisomal ABC Transporters / Noam Shani ; David Valle56.： |
| Mitochondrial ABC Transporters / Jonathan Leighton57.： |
| Contributors to Volume 295 |
| Deciphering Rules of Helix Stability in Peptides / Carol A. Rohl ; Robert L. Baldwin |
| Modified Amino Acids as Probes of Helix Stability / Neville R. Kallenbach ; Erik J. Spek |
| Measuring Hydration Changes of Proteins in Solution: Applications of Osmotic Stress and Structure-Based Calculations / Vince J. LiCata ; Norma M. Allewell |
| Protein Folding in Membranes: Determining Energetics of Peptide-Bilayer Interactions / Stephen H. White ; William C. Wimley ; Alexey S. Ladokhin ; Kalina Hristova |
| Tight Ligand Binding Affinities Determined from Thermodynamic Linkage to Temperature by Titration Calorimetry / Michael L. Doyle ; Preston Hensley |
| Structure-Based Prediction of Binding Affinities and Molecular Design of Peptide Ligands / Irene Luque ; Ernesto Freire |
| Molecular Crowding: Analysis of Effects of High Concentrations of Inert Cosolutes on Biochemical Equilibria and Rates in Terms of Volume Exclusion / Allen P. Minton |
| Application of Automated Methods for Determination of Protein Conformational Stability / Jeffery M. Schwehm ; Wesley E. Stites |
| Calculations of Proton-Binding Thermodynamics in Proteins / Paul Beroza ; David A. Case |
| Analysis of Spectra from Multiwavelength Oxygen-Binding Studies of Mixed Metal Hybrid Hemoglobins / Alexandra L. Klinger ; Gary K. Ackers |
| Study of the Bohr Effect in Hemoglobin Intermediates / Louise Benazzi ; Rosaria Russo ; Marilena Ripamonti ; Michele Perrella |
| Application of Planck-Benzinger Relationships to Biology / Paul W. Chun |
| Kinetic Analysis of Macromolecular Interactions Using Surface Plasmon Resonance Biosensors / Thomas A. Morton ; David G. Myszka |
| Prediction of Binding Energetics from Structure Using Empirical Parameterization / Brian M. Baker ; Kenneth P. Murphy |
| Photoacoustic Calorimetry of Proteins / Mark A. McLean ; Carmelo Di Primo ; Eric Deprez ; Gaston Hui Bon Hoa ; Stephen G. Sligar |
| Isoergonic Cooperativity: A Novel Form of Allostery / Harvey F. Fisher ; Jon Tally |
| Theoretical Aspects of Isothermal Titration Calorimetry / Lawrence Indyk |
| Use of Poisson-Boltzmann Equation to Analyze Ion Binding to DNA / Victor A. Bloomfield ; Ioulia Rouzina |
| Following the Folding of RNA with Time-Resolved Synchrotron X-Ray Footprinting / Bianca Sclavi ; Sarah Woodson ; Michael Sullivan ; Mark Chance ; Michael Brenowitz |
| Analysis of Interactions between CytR and CRP at CytR-Regulated Promoters / Donald F. Senear ; Laura T. Perini ; Stacey A. Gavigan |
| Energetic Methods to Study Bifunctional Biotin Operon Repressor / Dorothy Beckett |
| Thermal Melting Properties of C-Terminal Domain Mutants of Bacteriophage [lambda] cI Repressor / Eddine K. Merabet ; David S. Burz |
| Investigation of Phase Transitions in Bilayer Membranes / Jeffrey T. Mason |
| Membrane-Confined Analytical Electrophoresis / Thomas M. Laue ; Harvey K. Shepard ; Theresa M. Ridgeway ; Thomas P. Moody ; Timothy J. Wilson |
| Biological Activity / J.S. Alexander ; N. Okayama |
| Quantifying Role of Nitric Oxide in Endothelial Barrier Regulation / M.J. Eppihimer ; D.N. Granger |
| In Vivo Measurements of Endothelial Cell Adhesion Molecule Expression / P. Kubes |
| Ruthenium Red as Measure of Mast Cell Activation / M.K. Shigenaga |
| Quantitation of Protein-Bound 3-Nitrotyrosine by High-Performance Liquid Chromatography with Electrochemical Detection / H. Ohshima ; H. Bartsch |
| Quantitative Estimation of Endogenous N-Nitrosation in Humans by Monitoring N-Nitrosoproline in Urine / D.L. Granger ; N.M. Anstey ; W.C. Miller ; J.B. Weinberg |
| Measuring Nitric Oxide Production in Human Clinical Studies / J.E. Freedman ; J.F. Keaney, Jr. |
| Nitric Oxide and Superoxide Detection in Human Platelets / P. Martásek ; R.T. Miller ; L.J. Roman ; T. Shea ; B.S.S. Masters |
| Assay of Isoforms of Escherichia coli-Expressed Nitric Oxide Synthase / E.I. Closs ; G.E. Mann |
| Identification of Carrier Systems in Plasma Membrane of Mammalian Cells Involved in Transport of L-Arginine / P. Forte ; L.M. Smith ; E. Milne ; N. Benjamin |
| Measurement of Nitric Oxide Synthesis in Humans Using L-[ 15 N2]-Arginine / T.J. McMahon ; J.S. Stamler |
| Concerted Nitric Oxide/Oxygen Delivery by Hemoglobin / W.K. Alderton ; P.K. Lowe |
| Scintillation Proximity Assay to Measure Nitroarginine and Tetrahydrobiopterin Binding to Heme Domain of Neuronal Nitric Oxide Synthase / K.-D. Kröncke ; V. Kolb-Bachofen |
| Measurement of Nitric Oxide-Mediated Effects on Zinc Homeostasis and Zinc Finger Transcription Factors / L.A. MacMillan-Crow ; J.A. Thompson |
| Immunoprecipitation of Nitrotyrosine-Containing Proteins / Y. Ji ; T.P.M. Akerboom ; H. Sies ; J.A. Thomas |
| Gel Electrofocusing Method for Studying Protein S-Nitrosylation / J.P. Crow |
| Measurement and Significance of Free and Protein-Bound 3-Nitrotyrosine, Free 3-Nitro-4-hydroxyphenylacetic Acid, and 3-Chlorotyrosine in Biologic Samples: A High-Performance Liquid Chromatography Method Using Electrochemical Detection / S. Mesáros |
| Determination of Nitric Oxide Saturated Solution by Amperometry on Modified Microelectrode / J. Vásquez-Vivar ; P. Martasek ; N. Hogg ; H. Karoui ; B.S. Siler Masters ; K.A. Pritchard, Jr. ; B. Kalyanaraman |
| Electron Spin Resonance Spin-Trapping Detection of Superoxide Generated by Neuronal Nitric Oxide Synthase / R.M. Bolzan ; R. Cueto ; G.L. Squadrito ; R.M. Uppu ; W.A. Pryor |
| Direct and Simultaneous Ultraviolet Second-Derivative Spectrophotometric Determination of Nitrite and Nitrate in Preparations of Peroxynitrite / A. Boveris ; L.E. Costa ; E. Cadenas ; J.J. Poderoso |
| Regulation of Mitochondrial Respiration by Adenosine Diphosphate, Oxygen, and Nitric Oxide. Nitric Oxide Donors: Nitrosothiols and Nitroxyls / D.A. Wink ; S. Kim ; D. Coffin ; J.A. Cook ; Y. Vodovotz ; D. Chistodoulou ; D. Jourd'heuil ; M.B. Grisham |
| Detection of S-Nitrosothiols by Fluorometric and Colorimetric Methods / S.B. King ; H.T. Nagasawa |
| Chemical Approaches toward Generation of Nitroxyl / F.S. Laroux ; D. Kang ; A.M. Miles |
| Stability of S-Nitrosothiols in Presence of Copper, Zinc-Superoxide Dismutase / P. Kostka ; J.K.J. Park |
| Fluorometric Detection of S-Nitrosothiols / A.D. McGill ; Y. Yang ; J. Wang ; L. Echegoyen ; P.G. Wang |
| Redox-Sensitive Nitric Oxide Dono |
| Oxidative Damage to Lipids, Proteins and Nucleic Acids: Lipids / J.D. Morrow ; L.J. Roberts II |
| Mass Spectrometric Quantification of F2-Isoprostanes in Biological Fluids and Tissues as a Measure of Oxidant Stress / J. Nourooz-Zadeh |
| Improved GC-MS Assay for the Measurement of Plasma Isoprostanes / G.T. Shwaery ; H. Mowri ; B. Frei |
| Preparation of Lipid Hydroperoxide-Free LDL / W. Korytowski ; PG. Geiger ; A.W. Girotti |
| Lipid Hydroperoxide Analysis by High-Performance Liquid Chromatography with Mercury Cathode Electrochemical Detection / A.M. Pastorino ; A. Zamburlini ; L. Zennaro ; M. Maiorino ; F. Ursini |
| Measurement of Lipid Hydroperoxides in Human Plasma and Lipoproteins by Kinetic Analysis of Photon Emission / G.J. Handelman |
| HPLC Analysis of Cholesterol Linoleate Hydroperoxide in Oxidized LDL, with Calibration by Conjugated Diene Internal Standard / J.M. Samii |
| Determination of Phospholipid Oxidation in Cultured Cells |
| Ferrous Ion Oxidation in the Presence of Xylenol Orange for the Detection of Lipid Hydroperoxides in Plasma / T.R. Dugas ; D.F. Church |
| Purification and Characterization of Phospholipid for Use in Lipid Oxidation Studies / H.C. Yeo ; J.K. Liu ; H.J. Helbock ; K.B. Beckman ; B.N. Ames |
| Malondialdehyde Assay in Biological Fluids by Gas Chromatography-Mass Spectrometry / P.A. Glascott, Jr. ; J. Farber |
| Assessment of the Physiological Interaction Between Vitamin E and Vitamin C |
| Proteins and Nucleic Acids / S.L. Hazen ; F.F. Hsu ; J.P. Gaut ; J.R. Crowley ; J.W. Heinecke |
| Modification of Proteins and Lipids by Myeloperoxidase / C.C. Winterbourn ; H. Buss |
| Protein Carbonyl Measurement by ELISA / A.J. Kettle |
| Detection of 3-Chlorotyrosine in Proteins Exposed to Neutrophil Oxidants / S.A. Lewisch ; R.L. Levine |
| Determination of 2-Oxohistidine by Amino Acid Analysis / C. Leeuwenburgh ; D.M. Mueller ; J.E. Rasmussen ; J. Turk |
| Detecting Oxidative Modifications of Biomolecules with Isotope Dilution Mass Spectrometry: Sensitive and Quantitative Assays for Oxidized Amino Acids in Proteins and Tissues / P. Evans ; L. Lyras ; B. Halliwell |
| Measurement of Protein Carbonyls in Human Brain Tissue |
| 8-Hydroxydeoxyguanosine and 8-Hydroxyguanine as Biomarkers of Oxidative DNA Damage / S. Loft ; H.E. Poulsen |
| Markers of Oxidative Damage to DNA: Antioxidants and Molecular Damage / S.M. Piperakis ; E.E. Visvardis ; A.M. Tassiou |
| Comet Assay for Nuclear DNA Damage / A.J.S.C. Viera ; J.P. Telo ; R.M.B. Dias |
| Generation of Hydroxyl Radical by Photolysis of Mercaptopyridine N-Oxides: Application to the Redox Chemistry of Purines / B. Li ; P.L. Gutierrez ; N. Blough |
| Trace Determination of Hydroxyl Radical Using Fluorescence Detection |
| Antioxidant Assays in Cells, Body Fluids and Tissues / H.Z. Chae ; S.W. Kang ; S.G. Rhee |
| Isoforms of Mammalian Peroxiredoxin That Reduce Peroxides in the Presence of Thioredoxin / E.S.J. Arneacute;r ; L. Zhong ; A. Holmgren |
| Preparation and Assay of Mammalian Thioredoxin and Thioredoxin Reductase / J. Moskovitz ; B.S. Berlett ; J.M. Poston ; E.R. Stadtman |
| Methionine Sulfoxide Reductase in Antioxidant Defense / P. Venditti ; T. DeLeo ; S. DiMeo& |
| Total Antioxidant Activity / H. Alho ; J. Leinonen |
| Total Antioxidant Activity Measured by Chemiluminescence Methods / I.F.F. Benzie ; J.J. Strain |
| Ferric Reducing/Antioxidant Power Assay: Direct Measure of ""Total Antioxidant Activity"" of Biological Fluids, and a Modified Version (FRASC) Which Enables the Simultaneous Measurement of Total Antioxidant Power and Ascorbic Acid Concentration / Y. Noda ; M. Kohno ; A. Mori ; L. Packer |
| Automated ESR Free Radical Detector Assays for Antioxidant Activity in Natu |
| Analysis of Actomyosin-Related Systems / R.E. Cheney |
| Purification and Assay of Myosin / V. K. Ayscough |
| Use of Latrunculin-A, An Actin Monomer Binding Drug / M.R. Bubb ; I. Spector |
| Use of the F-Actin Binding Drugs, Misakinolide A and Swinholide A / E.J. Luna |
| F-Actin Blot Overlays / J.F. Kelleker ; R.D. Mullins ; T.D. Pollard |
| Purification and Assay of the Arp2/3 Complex from Acanthamoeba castellani / M.D. Welch |
| Purification and Assay of the Platelet Arp2/3 Complex / K.L. Schmeichel ; B.E. Stronach ; M.C. Beckerle |
| Purification and Assay of Zyxin / C.E. Turner ; M.C. Brown |
| Purification and Assays for Paxillin / J. Lacoste ; A. Ma ; J.T. Parsons |
| Assay and Purification of Focal Adhesion Kinase / T. Jarchau ; T. Mund ; M. Reinhard ; U. Walter |
| Purification and Assays of VASP / D.C. Fung |
| Listeria monocytogenes-based Assays for Actin Assembly Factors |
| Analysis of Microtubule-Related Systems / A. Desai |
| Preparation and Characterization of Caged Fluorescein Tubulin / D. Meyer ; D.R. Rines ; A. Kashina ; J.M. Scholey |
| Purification of Novel Kinesins from Embryonic Systems / D.W. Pierce ; R.D. Vale |
| Assaying Processive Movement of Kinesin by Fluorescence Microscopy / J.B. Bingham ; S.J. King ; T.A. Schroer |
| Purification of Dynactin and Dynein from Brain Tissue / M.P. Sheetz ; J. Kumar |
| Isolation and Characterization of Kinectin / G.S. Diamantopoulos ; T.E. Kreis ; J.E. Rickard |
| Purification and Assay of CLIP-170 / F. McNally |
| Purification and Assay of the Microtubule-Severing Protein, Katanin / M.L. Wong ; B. Alberts ; T. Mitchison |
| Purification and Assay of gTubulin Ring Complex / M. Blomberg-Wirschell ; S.J. Doxsey |
| Rapid Isolation of Centrosomes / G.A. Orr ; S. Rao ; C.S. Swindell ; D.G.I. Kingston ; S.B. Horwitz |
| Photoaffinity Labeling Approach to Map Taxol-Binding Site on the Microtubule / M.A. Jordan ; L.Wilson |
| Use of Drugs to Study Role of Microtubule Assembly Dynamics in Living Cells |
| Other Cytoskeletal Systems / K. Oegema ; C.M. Field |
| Purification and Assay of A Septin Complex from Drosophila Embryos / A. Mukherjee ; J. Lutkenhaus |
| Purification, Assembly and Localization of FtsZ / C. Lu ; H.P. Erickson |
| Purification and Assembly of FtsZ Cell-Free and Genetic Systems / E.D. Salmon ; E. Yeh ; S. Shaw ; B. Skibbens ; K. Bloom |
| High Resolution Video and Digital Enhanced Differential Interference Contrast Light Microscopy of Cell Division in Budding Yeast / D.A. Compton |
| Production of M-phase and I-phase Extracts from Mammalian Cells / V.J. Allan |
| Organelle Motility and Membrane Network Formation in Metaphase and Interphase Cell-Free Extracts / C. McGoldrick ; M. Sheetz |
| Organelle Motility and Membrane Network Formation from Cultured Mammalian Cells / S.L. Rogers ; I.S. Tint ; V.I. Gelfand |
| An in Vitro Motility Assay for Melanophore Pigment Organelles / J.K. Burkhardt |
| Purification of Phagosomes and Assays for Microtubule Binding / J. Scheel |
| Magnetic Bead Assay for Characterization of Microtubule-Membrane Interactions. &I |
| Genetic Approaches to Dissect Complex Functions / M.F. Cohen ; J.C. Meeks ; Y.A. Cai ; C.P. Wolk |
| Tranposon Mutagenesis of Heterocyst-Forming Filamentous Cyanobacteria / J. Yu ; L. McIntosh |
| Isolation and Genetic Characterization of Pseudorevertants from Site-Directed PSI Mutants in Synechocystis 6803 / K.L. Kindle |
| High-Frequency Transformation of Chlamyomonas reinhardtii / A. Barkan |
| Approaches to Investigating Nuclear Genes That Function in Chloroplast Biogenesis in Land Plants |
| Photosynthetic Complexes: Function/Structure / G. Fritzsch |
| Obatining Crystal Structures from Bacterial Photosynthetic Reaction Centers / H.G. Koch ; H. Myllykallio ; F. Daldal |
| Using Genetics to Explore Cytochrome Function and Structure in Rhodobacter / J.H. Goldbeck |
| Comparison of in Vitro and in Vivo Mutants of Photosystem I: Protocols for Mutagenesis and Techniques for Analysis / J. Sun ; A. Ke ; J. Ping ; V.P. Chitnis ; P.R. Chitnis |
| Isolation and Functional Study of Photosystem I Subunits in the Cyanobacterium Synechocystis sp. PCC 6803 / R.E. McCarty ; J.A. Cruz |
| Delineation of Critical Regions of the Epsilon Subunit of Chloroplast ATP Synthase Through a Combination of Biochemical and Site-Directed Mutagenesis Approaches |
| Gene Expression of Photosynthetic Components / J.H. Zeilstra-Ryalls ; M. Gomelsky ; A.A. Yeiliseev ; J.M. Eraso ; S. Kaplan |
| Transcriptional Regulation of Photosynthesis Operons in Rhodobacter sphaeroides 241 / C. Jansson ; G. Sailh ; J. Eriksson ; R. Wiklund ; H. Ghebramedhin |
| Use of Synechocystis 6803 to Study Expression of a psbA Gene Family / P.P. Dijkwel ; F. Rook ; S.C.M. Smeekens |
| Analysis of Light-Regulated Gene Expression / A. Cohen ; C.B. Yohn ; R.K. Bruick ; S.P. Mayfield |
| Translational Regulation of Chloroplast Gene Expression in Chlamydomonas reinhardtii / S. Rodermel |
| Use of Antisense Mutants to Study Regulation of Photosynthesis During Leaf Development / D.G. Durnford ; O. Prasil ; J.M. Escoubas ; P.G. Falkowski |
| Assessing the Potential for Chloroplast Redox Regulation of Nuclear Gene Expression |
| Biogenesis and Adaptation of Photosynthetic Components / G. Armstrong ; K. Apel |
| Molecular and Genetic Analysis of Light-Dependent Chlorophyll Biosynthesis / M. Harker ; J. Hirschberg |
| Molecular Biology of Carotenoid Biosynthesis in Photosynthetic Organisms / J.M. Quinn ; S. Merchant |
| Copper-Responsive Gene Expression During Adaptation to Copper-Deficiency / D.M. Kehoe ; A.R. Grossman |
| Using Molecular Genetics to Investigate Complementary Chromatic Adaptation: Advances in Transformation and Complementation |
| Photosynthetic Mutants: Construction and Biological/Biochemical/Biophysical Analyses / W.F.J. Vermaas |
| Gene Modification and Mutation Mapping to Study the Function of Photosystem II / H. Lee ; S.E. Bingham ; A.N. Webber |
| Specific Mutagenesis of Reaction Center Proteins by Chloroplast Transformation of Chlamydomonas reinhardtii / T.M. Bricker ; C. Putnam-Evans ; J. Wu |
| Directed Mutagenesis in Photosystem II: Analysis of the CP 47 Protein / B.A. Diner |
| Application of Spectroscopic Techniques to the Study of Mutations Engineered in Synechoceptis and Chlomydomona |
| Purification of Transporter Proteins and cDNAs: Plasma Membrane Carriers / C. Aragon ; B. Lopez-Corcuera |
| Purification, Hydrodynamic Properties, and Glycosylation Analysis of Glycine Transporters / M.F. Romero ; Y. Kanai ; H. Gunshin ; M.A. Hediger |
| Expression Cloning Using Xenopus laevis Oocytes / N. Nelson ; Q.R. Liu |
| Cloning of Genes of cDNAs Encoding Neurotransmitter Transporters and their Localization by Immunocytochemistry |
| Vesicular Carriers / R. Yelin ; S. Schuldiner |
| Purification of the Vesicular Monoamine Transporters: From Classical Techniques to Histidine Tags / J.P. Henry ; C. Sagneacute; ; M.F. Isambert ; B. Gasnier |
| Noncovalent and Covalent Labeling of the Vesicular Monoamine Transporter with Tetrabenazine and Ketanserin Derivatives; Purification of the Photolabeled Protein / H. Varoqui ; J.D. Erickson |
| Functional Identification of Vesicular Monoamine and Acetylcholine Transporters / S.M. Parsons ; G.A. Rogers ; L.M. Gracz |
| Photoaffinity Labeling of the Vesicular Acetylcholine Transporter from the Electric Organ of Torpedo / J.W. Hell ; R. Jahn |
| Bioenergetic Characterization of the GABA Transporter of Synaptic Vesicles / S.M. Lewis ; T. Ueda |
| Solubilization and Reconstitution of the Synaptic Vesicle Glutamate Transport System / J.P. Finn, III ; A. Merickel ; R.H. Edwards |
| Analysis of Neurotransmitter Transport into Secretory Vesicles |
| Pharmacological Approaches and Binding Studies / P. Krogsgaard-Larsen ; B.F. Froslash;lund ; E. Falch |
| Inhibitors of GABA Transport as Experimental Tools and Therapeutic Agents / A.R. Chamberlin ; H.P. Koch ; R.J. Bridges |
| Design and Synthesis of the Conformationally Constrained Inhibitors of the High-Affinity, Sodium-Dependent Glutamate Transporters / M. Robinson |
| Examination of Glutamate Transporter Heterogeneity Using Synaptosomal Preparations / A.T. Coorera ; J.C. Do Reacute;go ; J.J. Bonnet |
| Specificity and Ion Dependence of Binding of GBR Analogs / R.A. Vaughn |
| Cocaine and GBR Photoaffinity Ligands as Probes of Dopamine Transporter Structure |
| Transport Assays and Kinetic Analyses / G. Rudnick |
| Ion-Coupled Neurotransmitter Transport: Thermodynamic vs. Kinetic Determinations of Stoichiometry / M.E.A. Reith ; C. Xu ; F.I. Carroll ; N.H. Chen |
| Comparison of Inhibition of [ 3 H] Dopamine Translocation and [ 3 H] Cocaine Analog Binding: A Potential Screening Device for Cocaine Antagonists / H. Bönisch |
| Transport and Drug Binding Kinetics in Membrane Vesicle Preparations / V. Ganapathy ; P.D. Prasad ; F.H. Leibach |
| Use of Human Placenta in Studies of Monoamine Transporters |
| Biochemical Approaches for Structure-Function Analyses / J.A. Clark |
| Analysis of Transporter Topology Using Deletion and Epitope Tagging / G.M. Daniels ; S.G. Amara |
| Selective Labeling of Neurotransmitter Transporters at the Cell Surface / R.P. Seal ; B.H. Leighton |
| Transmembrane Topology Mapping Using Biotin-Containing Sulfhydryl Reagents / J.A. Javitch |
| Probing the Structure of Neurotransmitter Transporters by the Substituted-Cysteine Accessibility Method / S. Ramamoorthy ; H.E. Melikian ; Y. Qian ; R.D. Blakely |
| Biosynthesis, N-glycosylation, and Surface Tracking of Biogenic Amine Transporter Proteins / J. Ahn ; G. Pitrini ; T.R. Muth ; M.J. Caplan& |
| Refolding and Purification of Ras Proteins / S.L. Campbell-Burk ; J.W. Carpenter ; T. Evans ; G.E. Bollag ; R. Clark |
| Purification of Baculovirus-Expressed Recombinant Ras and Rap Proteins / F. McCormick |
| Purification of Recombinant Ras GTPase-Activating Proteins / B. Rubinfeld ; P. Polakis |
| Purification of Baculovirus-Produced Rap 1 GTPase-Activating Proteins / G.L. James ; M.S. Brown ; J.L. Goldstein |
| Assays for Inhibitors for CAAX Farnesyltransferase in Vitro and in Intact Cells / R. Khosravi-Far ; C.J. Der |
| Prenylation Analysis of Bacterially Expressed and Insect Cell-Expressed Ras and Ras-Related Proteins |
| Reticulocyte Lysate Assay for in Vitro Translation and Posttranslational Modification of Ras Proteins / C. Volker ; J.B. Stock |
| Carboxyl Methylation of Ras-Related Proteins / F. Tamanoi ; H. Mitsuzawa |
| Use of Yeast for Identification of Farnesyltransferase Inhibitors and for Generation of Mutant Farnesyltransferases |
| Analysis of Intrinsic and CDC25-Stimulated Guanine Nucleotide Exchange of p21 ras --Nucleotide Complexes by Fluorescence Measurements / C. Lenzen ; R.H. Cool ; A. Wittinghofer ; J. Downward |
| Measurement of Nucleotide Exchange and Hydrolysis Activities in Immunoprecipitates / J.B. Gibbs |
| Determination of Guanine Nucleotides Bound to Ras in Mammalian Cells / M. Frech ; D. Cussac ; P. Chardin ; D. Bar-Sagi |
| Purification of Baculovirus-Expressed Human Sos1 Protein / M. Hart ; S. Powers |
| Ras-Cdc25 and Rho-Dbl Binding Assays: Complex Formation in Vitro / R.D. Mosteller ; W. Park ; D. Broek |
| Analysis of Interaction between Ras and CDC25 Guanine Nucleotide Exchange Factor Using Yeast GAL4 Two-Hybrid System / T. Satoh ; Y. Kaziro |
| Measurement of Ras-Bound Guanine Nucleotide in Stimulated Hematopoietic Cells / A.M.M. de Vries-Smits ; L. van der Voorn ; J.L. Bos |
| Measurements of GTP/GDP Exchange in Permeabilized Fibroblasts |
| Intrinsic and GTPase-Activating Protein-Stimulated Ras GTPase Assays / M.D. Schaber |
| Determination of Ras and GTPase-Activating Protein Interactions by Kinetic Competition Assay / C. Ellis ; V. Measday ; M.F. Moran |
| Phosphorylation-Dependent Complexes of p120 Ras-Specific GTPase-Activating Proteins with p62 and p190 |
| Cell Expression and Analysis: in Vitro / A.D. Cox ; P.A. Solski ; J.D. Jordan |
| Analysis of Ras Protein Expression in Mammalian Cells / A.M.M. deVries-Smits ; B.M.T. Burgering |
| Vaccinia Virus Expression of p21ras Asn-17 / H. Cai ; G.M. Cooper |
| Inducible Expression of Ras N17 Dominant Inhibitory Protein |
| Prenylation and Palmitoylation Analysis / C.W.M. Reuter ; A.D. Catling ; M.J. Weber |
| Immune Complex Kinase Assays for Mitogen-Activated Protein Kinase and MEK / S. Kuroda ; K. Shimizu ; B. Yamamori |
| Cell-Free Assay System for Ras-Dependent MEK Activation / P. Dent ; G. Romero ; D. Castle ; T.W. Sturgill |
| Preparation and Use of Semiintact Mammalian Cells for Analysis of Signal Transduction / C.J |
| Contents of Volumes 195-198, 200-227 |
| cDNA Preparation / J. Estee ; P. Crino ; J. Eberwine |
| Preparation of cDNA from Single Cells and Subcellular Regions / P. Carninci ; Y. Hayashizaki |
| High-Efficiency Full-length cDNA Cloning / M. Liu ; Y.V.B.K. Subramanyam ; N. Baskaran |
| Preparation and Analysis of cDNA from a Small Number of Hematopoietic Cells / C. Aston ; C. Hiort ; D.C. Schwartz |
| Optical Mapping: An Approach for Fine Mapping |
| Gene Identification / R.J. Mural |
| Current Status of Computational Gene Finding: A Perspective / D.M. Church ; A.J. Buckler |
| Gene Identification by Exon Amplification / J.T. den Dunnen |
| Cosmid-Based Exon Trapping / A.D. Simmons ; M. Lovett |
| Direct cDNA Selection Using Large Genomic DNA Targets / S. Parimoo ; S.M. Weissman |
| cDNA Selection: An Approach for Isolation of Chromosome Specific cDNAs / K. Gardiner |
| Saturation Identification of Coding Sequences in Genomic DNA |
| Patterns of mRNA Expression / R. Drmanac ; S. Drmanac |
| cDNA Screening by Array Hybridization / M.B. Eisen ; P.O. Brown |
| DNA Arrays for Analysis of Gene Expression / M.D. Clark ; G.D. Panopoulou ; D.J. Cahill ; K. Büssow ; H. Lehrach |
| Construction and Analysis of Arrayed cDNA Libraries / K.J. Martin ; A.B. Pardee |
| Principles of Differential Display / Y. Prashar |
| A Method for Display of 3'-End Fragments of Restriction Enzyme Digested cDNAs for Analysis of Differential Gene Expression / Y.V.B.K. Subrahmkanyam ; P.E. Newburger |
| A Modified Method for the Display of 3'-End Restriction Fragments of cDNAs: Molecular Profiling of Gene Expression in Neutrophils / T. Ito ; Y. Sakaki |
| Fluorescent Differential Display (FDD): A Fast and Reliable Method for Message Display PCR / F. Mathieu-Daudé ; T. Trenkle ; J. Welsh ; B. Jung ; T. Vogt ; M. McClelland |
| Identification of Differentially Expressed Genes Using RNA Fingerprinting by Arbitrarily Primed PCR / M. Hubank ; D.G. Schatz |
| cDNA RDA: A Sensitive and Flexible Method for the Identification of Differentially Expressed Genes / L. Diatchenko ; S. Lukyanov ; Y.-F.C. Lau ; P.D. Siebert |
| Suppression Subtractive Hybridization: A Versatile Method for Identifying Differentially Expressed Genes / F. Mathieu-Daudeacute; |
| Reduced Complexity Probes for DNA Arrays |
| Alastair / J.H. Brown |
| Catherine Hutchings / J.F. Burke ; L.V. Mayne |
| Targeted Display, A New Technique for the Analysis of Differential Gene Expression |
| Functional Relationship Among cDNA Translation Productions / M.-C. Marsolier ; A. Sentenac |
| Pol III-Based Two-Hybrid System / F.J. Germino ; N.K. Moskowitz |
| Screening for Protein-Protein Interactions / N.L. Sticker ; M. Li |
| Using the Lac Repressor System to Identify Interacting Proteins / K.A. Jacobs ; L.A. Collins-Racie ; M. Colbert ; M. Duckett ; C. Evans ; M. Golden-Fleet ; K. Kelleher ; R. Kriz ; E.R. LaVallie ; D. Merberg ; V. Spaulding ; J. Stover ; M.J. Williamson ; J.M. McCoy |
| A Genetic Selection for Isolating cDNA Clones That Encode Signal Peptides / K. Tashiro ; T. Nakamura ; T. Honjo |
| The Signal Sequence Trap Method / S. Staring;hl ; J. Odeberg ; M. Larsson ; Oslash;. Roslash;sok ; A.H. Ree ; J. Lundeberg |
| Solid-Phase Differential Display and Bacterial Expression Systems in Selection and Functional Analysis o |
| Hydrocarbons and Related Compounds |
| Enzymes of Alkane and Alkene Utilization / S.W. May ; A.G. Katopodis |
| Hydrocarbon Monooxygenase System of Pseudomonas oleovorans / W.R. Finnerty |
| Assay Methods for Long-Chain Alkane Oxidation in Acinetobacter |
| Primary Alcohol Dehydrogenases from Acinetobacter |
| Aldehyde Dehydrogenases from Acinetobacter / W. Ashraf ; J.C. Murrell |
| Propane-Specific Alcohol Dehydrogenase from Rhodococcus rhodochrous PNKb1 |
| Cell-Free Assay Methods for Enzymes of Propane Utilization / B.W. Groen ; J.A. Duine |
| Quinoprotein Alcohol Dehydrogenase from Pseudomonas aeruginosa and Quinhemoprotein Alcohol Dehydrogenase from Comamonas testosteroni (Pseudomonas testosteroni) |
| Enzymes Involved in Cyclic and Aromatic Hydrocarbon Utilization / L.P. Wackett |
| Toluene Dioxygenase from Pseudomonas putida F1 / B.D. Ensley ; B.E. Haigler |
| Naphthalene Dioxygenase from Pseudomonas NCIB 9816 / P.J. Geary ; J.R. Mason ; C.L. Joannou |
| Benzene Dioxygenase from Pseudomonas putida ML2 (NCIB 12190) / C.J. Batie ; D.P. Ballou |
| Phthalate Dioxygenase / P.W. Trudgill |
| Cyclohexanone 1,2-Monooxygenase from Acinetobacter NCIMB 9871 |
| Cyclohexanone 1,2-Monooxygenase from Pseudomonas NCIMB 9872 / J.W. Whittaker ; A.M. Orville ; J.D. Lipscomb |
| Protocatechuate 3,4-Dioxygenase from Brevibacterium fuscum / D.M. Arciero |
| Protocatechuate 4,5-Dioxygenase from Pseudomonas testosteroni / S.A. Wolgel |
| Protocatechuate 2,3-Dioxygenase from Bacillus macerans / M.R. Harpel |
| Gentisate 1,2-Dioxygenase from Pseudomonas acidovorans |
| Synthesis of 17O- or 18O-Enriched Dihydroxy Aromatic Acids / I.A. Kataeva ; L.A. Golovleva |
| Catechol 2,3-Dioxygenases from Pseudomonas aeruginosa 2X / K.-L. Ngai ; E.L. Neidle ; L.N. Ornston |
| Catechol and Chlorocatechol 1,2-Dioxygenases / R.B. Meagher |
| Muconate Cycloisomerase |
| Muconolactone Isomerase |
| Cis-1,2-Dihydroxycyclohexa-3,5-diene (NAD) Oxidoreductase (cis-Benzene Dihydrodiol Dehydrogenase) from Pseudomonas putida / B. Entsch |
| Hydroxybenzoate Monooxygenase / C.E. Cerniglia ; M.A. Heitkamp |
| Polycyclic Aromatic Hydrocarbon Degradation by Mycobacterium / J. Gibson ; J. Geissler ; C.S. Harwood |
| Benzoate-CoA Ligase from Rhodopseudomonas palustris |
| Yeast and Fungal Enzymes of Hydrocarbon Degradation / K. Kirk ; M. Tien ; P.J. Kersten ; K.E. Hammell ; R.L. Farrell |
| Lignin Peroxidase from Fungi: Phanerochaeti chrysosporium / M. Ueda ; A. Tanaka |
| Long-Chain Alcohol Dehydrogenase of Candida Yeast |
| Methylotrophy: Dissimilatory Enzymes of Methylotrophic Bacteria / S.J. Pilkington ; H. Dalton |
| Soluble Methane Monooxygenase from Methylococcus capsulatus Bath / B.G. Fox ; W.A. Froland ; D.R. Jollie |
| Methane Monooxygenase from Methylosinus trichosporium OB3b / J. Frank |
| Methanol Dehydrogenase from Hyphomicrobium X / D. Day ; C. Anthony |
| Methanol Dehydrogenase from Methylobacterium extorquens AM1 / A.R. Long ; Modi |
| The Biosynthesis of Phospholipids. Strategies for Generating Phospholipid Synthesis Mutants / E.A. Dennis |
| Strategies for Generating and Utilizing Phospholipid Synthesis Mutants in Escherichia coli / W. Dowhan |
| Strategies for Generating Phospholipid Synthesis Mutants in Yeast / M.J. Swede ; K.A. Hudak ; J.M. Lopes ; S.A. Henry |
| Strategies for Isolating Somatic Cell Mutants Defective in Lipid Biosynthesis of Plasmalogens / R.A. Zoeller ; C.R.H. Raetz |
| Acyltransferases / M.A. Scheideler ; R.M. Bell |
| Glycerophosphate Acyltransferase from Escherichia coli / D. Haldar ; A. Vancura |
| Glycerophosphate Acyltransferase from Liver / T. Sugiura ; K. Waku |
| Coenzyme A-Independent Acyltransferase / P.C. Choy ; P.G. Tardi ; J.J. Mukherjee |
| Lysophosphatidylcholine Acyltransferase / C.R. McMaster |
| 1-Alkyl- and 1-Alkenylglycerophosphocholine Acyltransferases / K.O. Webber ; A.K. Hajra |
| Dihydroxyacetone Phosphate Acyltransferase / R.A. Coleman |
| Diacylglycerol Acyltransferase and Monoacylglycerol Acyltransferase from Liver and Intestine / K.Y. Hostetler ; S.J. Huterer ; J.R. Wherrett |
| Biosynthesis of Bis(monoacylglycero)phosphate in Liver and Macrophage Lysosomes / S. Jackowski ; L. Hsu ; C.O. Rock |
| 2-Acylglycerophosphoethanolamine Acyltransferase/Acyl-[Acyl-Carrier-Protein] Synthetase from Escherichia coli. Kinases: K / Ishidate ; Y. Nakazawa |
| Choline/Ethanolamine Kinase from Rat Kidney / T.J. Porter ; C. Kent |
| Choline/Ethanolamine Kinase from Rat Liver / T. Uchida ; S. Yamashita |
| Choline/Ethanolamine Kinase from Rat Brain / J.P. Walsh |
| Diacylglycerol Kinase from Escherichia coli / H. Kanoh ; F. Sakane ; K. Yamada |
| Diacylglycerol Kinase Isozymes from Brain and Lymphoid Tissues / R.N. Lemaitre ; J.A. Glomset |
| Arachidonoyl-Specific Diacylglycerol Kinase / G.M. Carman ; C.J. Belunis ; J.T. Nickels Jr. |
| Phosphatidylinositol 4-Kinase from Yeast / C.E. Bazenet ; R.A. Anderson |
| Phosphatidylinositol-4-phosphate 5-Kinases from Human Erythrocytes / A. Moritz ; J. Westerman ; P.N.E. De Graan ; K.W.A. Wirtz |
| Phosphatidylinositol 4-Kinase and Phosphatidylinositol-4-phosphate 5-Kinase from Bovine Brain Membranes / F. Snyder |
| Alkylglycerol Phosphotransferase |
| Phosphatases / J.J. McLaughlin |
| Phosphatidate Phosphatase from Yeast Mitochondria / C.R. Funk |
| Phosphatidylglycerophosphate Phosphatase from Escherichia coli / T.C. Lee |
| 1-Alkyl-2-acetyl-sn-glycero-3-phosphate Phosphatase |
| Cytidylyltransferases / C.P. Sparrow |
| Purification of CDPdiacylglycerol Synthase from Escherichia coli / M.J. Kelley |
| CDPdiacylglycerol Synthase from Yeast / P.A. Weinhold ; D.A. Feldman |
| Choline-phosphate Cytidylyltransferase / L.B.M. Tijburg ; P.S. Vermeulen ; L.M.G.V. Golde |
| Ethanolamine-phosphate Cytidylyltransferase |
| Phosphocholine/Phosphoethanolamine Phosphotransferases / R.B. Cornell |
| Cholinephosphotransferase from Mammalian Sources / R.H. Hjelmstad |
| Choline- and Ethanolaminephosphotransferases from Saccharomyces cer |
| General Methodology / L.M. Silver |
| Recordkeeping and Database Analysis of Breeding Colonies / P.A. Flecknell |
| Anesthesia and Perioperative Care. Germ Cells and Embryos / J.E. Cooke ; I. Godin ; C. French-Constant ; J. Heasman ; C.C. Wylie |
| Culture and Manipulation of Primordial Germ Cells / M. Buehr ; A. McLaren |
| Isolation and Culture of Primordial Germ Cells / J.J. Eppig ; E.E. Telfer |
| Isolation and Culture of Oocytes / A.R. Bellve |
| Purification, Culture, and Fractionation of Spermatogenic Cells / W. Zheng ; Y.S. Martinova |
| Recovery, Capacitation, Acrosome Reaction, and Fractionation of Sperm / R.A. Cardullo ; H.M. Florman |
| Strategies and Methods for Evaluating Acrosome Reaction / J.A. Lawits ; J.D. Biggers |
| Culture of Preimplantation Embryos / K. Sturm ; P.P.L. Tam |
| Isolation and Culture of Whole Postimplantation Embryos and Germ Layer Derivatives |
| Histogenetic Potency of Embryonic Tissues in Ectopic Sites. Fertilization / J.W. Gordon |
| Micromanipulation of Gametes and Embryos / L.R. Fraser |
| In Vitro Capacitation and Fertilization / J.D. Bleil |
| In Vitro Fertilization. Gene Expression: Messenger RNA / K.B. Clegg ; L. Pike |
| Radioisotope Labeling of UTP and ATP Pools and Quantitative Analysis of RNA Synthesis / K.D. Taylor ; Lajos Pike |
| Quantitative Analysis of Specific Messenger RNAs by Hybridization Using RNA Probes / R.A. Kinloch ; R.J. Roller ; P.M. Wassarman |
| Quantitative Analysis of Specific Messenger RNAs by Ribonuclease Protection / M.Y. Arcellana-Panlilio ; G.A. Schultz |
| Analysis of Messenger RNA / M.F. Gaudette ; Q.P. Cao ; W.R. Crain |
| Quantitative Analysis of Specific Messenger RNAs in Small Numbers of Preimplantation Embryos / J. Singer-Sam ; A.D. Riggs |
| Quantitative Analysis of Messenger RNA Levels: Reverse Transcription-Polymerase Chain Reaction Single Nucleotide Primer Extension Assay / F.J. Salles ; W.G. Richards ; J. Huarte ; J.-D. Vassali ; S. Strickland |
| Microinjecting Antisense Sequences into Oocytes / D.G. Wilkinson ; M.A. Nieto |
| Detection of Messenger RNA by in Situ Hybridization to Tissue Sections and Whole Mounts / R.A. Conlon ; B.G. Herrmann |
| Detection of Messenger RNA by in Situ Hybridization to Postimplantation Embryo Whole Mounts / D. Sassoon ; N. Rosenthal |
| Detection of Messenger RNA by in Situ Hybridization. Gene Expression: Reporter Genes / M. Miranda ; M.L. DePamphilis |
| Preparation of Injection Pipettes / S. Majumder ; M. Wiekowski |
| Application of Firefly Luciferase to Preimplantation Development / M. Vernet ; C. Bonnerot ; P. Briand ; J.-F. Nicolas |
| Application of LacZ Gene Fusions to Preimplantation Development |
| Application of LacZ Gene Fusions to Postimplantation Development. Gene Expression: Proteins / K.E. Latham ; J.I. Garrels ; D. Solter |
| Two-Dimensional Gel Analysis of Protein Synthesis |
| One-Dimensional Gel Analysis of Histone Synthesis / C.A. Davis |
| Whole-Mount Immunohistochemistry / C.<$$$> |
| Monitoring of Physiological Processes / S. Kupzig ; S. San Lee ; G. Banting |
| Membrane Trafficking / C. Kaether ; H.-H. Gerdes |
| Monitoring of Protein Secretion with Green Fluorescent Protein / R.M. Hoffman |
| Green Fluorescent Protein to Visualize Cancer Progression and Metastasis / X. Zhao ; T. Duong ; C.-C. Huang ; S.R. Kain ; X. Li |
| Comparison of Enhanced Green Fluorescent Protein and Its Destabilized Form as Transcription Reporters / J.-T. Ma |
| Early Detection of Apoptosis with Annexin V-Enhanced Green Fluorescent Protein / M. Maniak |
| Green Fluorescent Protein in the Visualization of Particle Uptake and Fluid-Phase Endocytosis / F. Hanakam ; G. Gerisch |
| Monitoring Intracellular Shuttling of Histidine-rich pH-Sensor Proteins Tagged with Green Fluorescent Protein / S.R. Cronin ; R.Y. Hampton |
| Measuring Protein Degradation with Green Fluorescent Protein / G.L. Hager |
| Studying Nuclear Receptors with Green Fluorescent Protein Fusions |
| Localization of Molecules / M.R. Flory ; T.N. Davis |
| Localization of Calmodulin in Budding Yeast and Fission Yeast Using Green Fluorescent Protein / K.R. Olson ; J.B. Olmsted |
| Analysis of Microtubule Organization and Dynamics in Living Cells Using Green Fluorescent Protein-MAP4 Chimeras / V. Georget ; B. Terouanne ; J.-C. Nicholas ; C. Sultan |
| Trafficking of the Androgen Receptor / C.D. Webb ; O. Resnekov |
| Use of Green Fluorescent Protein for Visualization of Cell-Specific Gene Expression and Subcellular Protein Localization in Bacillus subtilis / L.S. Barak ; J. Zhang ; S.S.G. Ferguson ; M.G. Caron |
| Signaling, Desensitization, and Trafficking of G Protein Coupled Receptors Revealed by Green Fluorescent Protein Conjugates / G.A. Dabiri ; J.C. Ayoob ; K.K. Turnacioglu ; J.M. Sanger ; J.W. Sanger |
| Use of Green Fluorescent Proteins Linked to Cytoskeletal Proteins to Analyze Myofibrillogenesis in Living Cells |
| Special Uses / L. Lybarger ; R. Chervenak |
| Use of Fluorescent Proteins in Single and Multicolor Flow Cytometry / R. Chervanak |
| Flow Cytometric Analysis of Transcription: Use of Green Fluorescent Protein Variants to Control Transfection Efficiency / Y. Fang |
| Use of Co-expressed Enhanced Green Fluorescent Protein as a Marker for Identifying Transfected Cells / L.A.C. Blair ; K.K. Bence ; J. Marshall |
| The Jellyfish Green Fluorescent Protein: A Tool for Studying Ion Channels and Second Messenger Signaling in Neurons / A. Miyawaki ; J.M. Matheson ; L.G. Sayers ; A.Muto, T. Michikawa ; T. Furuichi ; K. Mikoshiba |
| Expression of Green Fluorescent Protein and Inositol 1,4,5-Trisphosphate Receptor in Xenopus laevis Oocytes / T. Takada ; K. Yoshida ; K. Nakamura ; G. Tsujimoto ; M. Katsuki ; S. Sugano |
| Expression of Green Fluorescent Protein in Transgenic Mice / A.S. Verkman |
| Green Fluorescent Protein as a Probe to Study Intracellular Solute Diffusion / T. Aoki ; K.S. Koch ; H.L. Leffert ; H. Watabe |
| Application of Green Fluorescent Protein-Protein A Fusion Protein to Western Blotting / P. Prabhakara ; M. Kallio ; J.C. Herr |
| Green Fluorescent Protein As a Reporter for Promoter Analysis of Testis-Specific Genes in Transgenic Mice / L.E. Bermudez ; F.J. Sangari ; A. Parker |
| Use of Green Fluorescent Protein for the Measurement of Bacteria-Host Interactions / D.W. Galbraith ; Fl |
