| Core Techniques / Volume 1： |
| Techniques for transformation of E.coli / 1： |
| Lambda replacement vectors in the construction of genomic DNA libraries / 2： |
| Procedures for cDNA cloning / 3： |
| Making probes / 4： |
| Using cloned DNAs to analyse gene organization and expression / 5： |
| Choices of single-stranded vectors and their application for in vitro mutagenesis / 6： |
| PCR and its applications / 7： |
| DNA sequencing / 8： |
| Expression Systems / Volume 2： |
| Screening expression libraries with antibody and protein probes |
| Expression of foreign proteins in E.coli using plasmid vectors and purification of specific polyclonal antibodies |
| Purification of over-produced proteins from E.coli cells |
| Production of monoclonal antibodies against proteins expressed in E.coli |
| Expression of cloned genes in yeast |
| Intraction trap cloning with yeast |
| The baculovirus expression system |
| Complex Genomes / VOLUME 3： |
| Cosmid clones and their application to genome studies |
| Chromosome-specific gridded cosmid libraries: construction, handling, and use in parallel and integrated mapping |
| Library construction in P1 phage vectors |
| Cloning into yeast artificial chromosomes |
| Amplification of DNA microdissected from mitotic and polytene chromosomes |
| Databases, computer networks, and molecular biology |
| Long-range restriction mapping |
| Genetic mapping with microsatellites |
| Mammalian Systems / Volume 4： |
| High efficiency gene transfer into mammalian cells |
| Construction and characterization of Vaccinia virus recombinants |
| Use of vectors based on gene amplification for the expression of cloned genes in mammalian cells |
| Retroviral vectors |
| Genetic manipulation of embryonic stem cells |
| Production of transgenic rodents by microinjection of cloned DNA into fertilized one-cell eggs |
| Genomic and expression analysis of transgenic animals |
| Expression using a defective Herpes simplex virus (HSV-1) vector system |
| Adenovirus vectors / 9： |
| Core Techniques / Volume 1： |
| Techniques for transformation of E.coli / 1： |
| Lambda replacement vectors in the construction of genomic DNA libraries / 2： |
| Procedures for cDNA cloning / 3： |
| Making probes / 4： |
| Using cloned DNAs to analyse gene organization and expression / 5： |
