Introduction to Microfluidics / Chapter 1: |
Abstract |
History of Microfluidics / 1.1: |
The beginning: Gas chromatography and capillary electrophoresis / 1.2.1: |
The microfluidic advantage / 1.2.2: |
Modular separation, reaction and hybridization systems / 1.2.3: |
Integrated systems / 1.2.4: |
Fluidics and Transport Fundamentals / 1.3: |
The continuum approximation / 1.3.1: |
Laminar flow / 1.3.2: |
Diffusion in microfluidic systems / 1.3.3: |
Surface forces and droplets / 1.3.4: |
Pumps and valves / 1.3.5: |
Electrokinetics / 1.3.6: |
Thermal management / 1.3.7: |
Device Fabrication / 1.4: |
Materials / 1.4.1: |
Fabrication and assembly / 1.4.2: |
Biological Applications / 1.5: |
Genetic analysis (DNA/RNA) / 1.5.1: |
Proteomics / 1.5.2: |
Cellular assays / 1.5.3: |
Drug delivery and compatibility / 1.5.4: |
The Future / 1.6: |
Potential demand/market for microfluidic devices / 1.6.1: |
Current products / 1.6.2: |
Challenges and the future / 1.6.3: |
References |
Materials and Microfabrication Processes for Microfluidic Devices / Chapter 2: |
Introduction / 2.1: |
Silicon Based Materials / 2.2: |
Micromachining of silicon / 2.2.1: |
Bulk micromachining / 2.2.2: |
Surface micromachining / 2.2.3: |
Glass Based Materials / 2.3: |
Microfabrication in glass / 2.3.1: |
Wafer Bonding / 2.4: |
Fusion bonding / 2.4.1: |
Anodic bonding / 2.4.2: |
Adhesive bonding / 2.4.3: |
Polymers / 2.5: |
Microfabrication / 2.5.1: |
Polymer materials / 2.5.2: |
Conclusion / 2.6: |
Interfacing Microfluidic Devices with the Macro World / Chapter 3: |
Typical Requirements for Microfluidic Interfaces / 3.1: |
Review of Microfluidic Interfaces / 3.3: |
World-to-chip interfaces / 3.3.1: |
Chip-to-world interfaces / 3.3.2: |
Future Perspectives / 3.4: |
Genetic Analysis in Miniaturized Electrophoresis Systems / Chapter 4: |
Status of genetic analyses / 4.1: |
Genetic analysis by miniaturized electrophoresis system / 4.1.2: |
Microchip Electrophoresis for Genomic Analysis / 4.2: |
Material and fabrication of electrophoresis microchips / 4.2.1: |
Theory of gel electrophoresis of DNA / 4.2.2: |
Gel matrices / 4.2.3: |
Novel DNA separation strategies on microchips / 4.2.4: |
Surface coating methods for microchannel walls / 4.2.5: |
Parallelization in Microchip Electrophoresis / 4.3: |
Integration in Microchip Electrophoresis for Genetic Analysis / 4.4: |
Sample preparation on microchip / 4.4.1: |
System integration / 4.4.2: |
Commercial Microfluidic Instruments for Genetic Analyses / 4.5: |
Commercial microchip electrophoresis instruments for genetic analysis / 4.5.1: |
Integrated microfluidic instruments for genetic analyses / 4.5.2: |
Microfluidic Markets and Future Perspectives / 4.6: |
Microfluidic Systems for Protein Separations / Chapter 5: |
Advantages of microfluidic chips for protein separations / 5.1: |
Limitations of microfluidic chips in proteomics applications / 5.1.2: |
Substrates used for proteomic analysis / 5.1.3: |
Microfluidic Chips for Protein Separation / 5.2: |
Microchip-based electrophoretic techniques / 5.2.1: |
Microchip chromatography / 5.2.2: |
Integrated Analysis in Microchips / 5.3: |
Integration of sample preparation with analysis / 5.3.1: |
Multi-dimensional separation in microchips / 5.3.2: |
Chips integrated with mass spectrometry / 5.3.3: |
Future Directions / 5.4: |
Microfluidic Systems for Cellular Applications / Chapter 6: |
Physiological advantages / 6.1: |
Biological advantages / 6.1.2: |
Economical advantages / 6.1.3: |
Microfluidic Technology for Cellular Applications / 6.2: |
Microfluidic cell isolation/separation / 6.2.1: |
Microfluidic cell culture / 6.2.2: |
Microfluidic cell analysis / 6.2.3: |
Commercialization of Microfluidic Technology / 6.3: |
Concluding Remarks / 6.4: |
Microfluidic Systems for Engineering Vascularized Tissue Constructs / Chapter 7: |
Generating 2D Vascularized Tissue Constructs Using Microfluidic Systems / 7.1: |
Generating 3D Vascularized Tissue Constructs Using Microfluidic Systems / 7.3: |
Hydrogel-based Microfluidic Systems for Generating Vascularized Tissue Constructs / 7.4: |
Mathematical Modeling to Optimize the Microfluidic Systems for Generating Vascularized Tissue Constructs / 7.5: |
Future Challenges / 7.6: |
Conclusions / 7.7: |
High Throughput Screening Using Microfluidics / Chapter 8: |
Cell-Based Assays / 8.1: |
High throughput cell culture / 8.2.1: |
Cell sorting for high throughput applications / 8.2.2: |
Biochemical Assays / 8.3: |
PCR / 8.3.1: |
Electrophoresis / 8.3.2: |
Others / 8.3.3: |
Drug Screening Applications / 8.4: |
Users and Developers of [mu]F HTS Platforms / 8.5: |
Users: Research labs, academic screening facilities, and pharmaceutical / 8.5.1: |
Commercialized products in HTS / 8.5.2: |
Acknowledgements / 8.6: |
Microfluidic Diagnostic Systems for the Rapid Detection and Quantification of Pathogens / Chapter 9: |
Infectious pathogens and their prevalence / 9.1: |
Traditional pathogen detection methods / 9.1.2: |
Microfluidic techniques / 9.1.3: |
Review of Research / 9.2: |
Pathogen detection/quantification techniques based on detecting whole cells / 9.2.1: |
Pathogen detection/quantification techniques based on detecting metabolites released or consumed / 9.2.2: |
Pathogen detection/quantification through microfluidic immunoassays and nucleic acid based detection platforms / 9.2.3: |
Future Research Directions / 9.3: |
Microfluidic Applications in Biodefense / Chapter 10: |
Biodefense Monitoring / 10.1: |
Civilian biodefense / 10.2.1: |
Military biodefense / 10.2.2: |
Current Biodefense Detection and Identification Methods / 10.3: |
Laboratory detection / 10.3.1: |
Field detection / 10.3.2: |
Microfluidic Challenges for Advanced Biodefense Detection and Identification Methods / 10.4: |
Microscale Sample Preparation Methods / 10.5: |
Spore disruption / 10.5.1: |
Pre-separations / 10.5.2: |
Nucleic acid purifications / 10.5.3: |
Immunomagnetic Separations and Immunoassays / 10.6: |
Immunomagnetic separations / 10.6.1: |
Immunoassays / 10.6.2: |
Proteomic Approaches / 10.7: |
Nucleic Acid Amplification and Detection Methods / 10.8: |
PCR and qPCR detection of pathogens for biodefense / 10.8.1: |
Miniaturized and Microfluidic PCR / 10.8.2: |
Heating and cooling approaches / 10.8.3: |
Miniaturized PCR and qPCR for biodefense / 10.8.4: |
Other Nucleic acid amplification methods / 10.8.5: |
Microarrays / 10.9: |
Microarrays and microfluidics / 10.9.1: |
Microelectrophoresis and Biodefense / 10.10: |
Microelectrophoresis technologies / 10.10.1: |
Integrated lab-on-a-chip systems and biodefense / 10.11: |
Full microfluidic integration for biodefense / 10.11.1: |
Summary and Perspectives / 10.12: |
Current and Future Trends in Microfluidics within Biotechnology Research / Chapter 11: |
The Past - Exciting Prospects / 11.1: |
The Present - Kaleidoscope-like Trends / 11.2: |
Droplet microfluidics / 11.2.1: |
Integrating Active Components in Microfluidics / 11.2.2: |
Third world - paper microfluidics - George Whitesides / 11.2.3: |
Microfluidic solutions for enhancing existing biotechnology platforms / 11.2.4: |
Microfluidics for cell biology - seeing inside the cell with molecular probes / 11.2.5: |
Microfluidics for cell biology - high throughput platforms / 11.2.6: |
The Future - Seamless and Ubiquitous MicroTAS / 11.3: |
Index |
Introduction to Microfluidics / Chapter 1: |
Abstract |
History of Microfluidics / 1.1: |
The beginning: Gas chromatography and capillary electrophoresis / 1.2.1: |
The microfluidic advantage / 1.2.2: |
Modular separation, reaction and hybridization systems / 1.2.3: |