| Assembly / J. Xu ; M. Li |
| Methods Used to Study Subunit Assembly of Potassium Channels / Z.F. Sheng ; C. Deutsch |
| Assembly of Ion Channels / J.S. Trimmer |
| Analysis of K + Channel Biosynthesis and Assembly in Transfected Mammalian Cells |
| Genetics / T.M. Ishii ; P. Zerr ; X-m. Xia ; C.T. Bond ; J. Maylie ; J.P. Adelman |
| Site-Directed Mutagenesis / S. Richard ; P. Lory ; E. Bourinet ; J. Nargeot |
| Molecular Physiology of Human Cardiovascular Ion Channels: From Electrophysiology to Molecular Genetics / R.L. Nakamura ; R.F. Gaber |
| Studying Ion Channels Using Yeast Genetics / M. Tiethammer ; M. Sheng |
| Identification of Ion Channel-Associated Proteins Using Yeast Two-Hybrid System / A. Karlin ; M.H. Akabas |
| Substituted-Cysteine Accessibility Method / R. Horn |
| Explorations of Voltage-Dependent Conformational Changes Using Cysteine Scanning / K. Sato ; M. Tohyama |
| Assessment of Distribution of Cloned Ion Channels in Neuronal Tissues |
| Electrophysiology / J.W. Hanrahan ; Z. Kone ; C.J. Mathews ; J. Luo ; Y. Jia ; P. Linsdell |
| Patch Clamp Studies of Cystic Fibrosis Transmembrane Conductance Regulator Chloride Channel / T.J. Nelson ; P.A. Gusev ; D.L. Alkon |
| Identification of Ion Channel Regulating Proteins by Patch Clamp Analysis / S.R. Lockery ; M.B. Goodman |
| Tight-Seal Whole-Cell Patch Clamping of Caenorhabditis elegans Neurons / R.A. Levis ; J.L. Rae |
| Low Noise Patch Clamp Techniques / D.N. Hilgemann ; C.C. Lu |
| Giant Membrane Patches: Improvements and Applications / W. Stühmer |
| Electrophysiological Recordings from Xenopus Oocytes / E. Stefani ; F. Bezanilla |
| Cut-Open Oocyte Voltage Clamp Technique / S. Kaneko ; A. Akaike ; M. Satoh |
| Cut-Open Recording Techniques |
| Gating Currents / J.G. Borst ; F. Helmchen |
| Calcium Influx During Action Potentials / L.D. Partridge ; H.U. Zeilhofer ; D. Swandulla |
| Combined Whole-Cell and Single-Channel Current Measurement with Quantitative Ca 2+ Injection or Fura-2 Measurement of Ca 2+ / T. Begenisich |
| Determining Ion Channel Permeation Properties / R. Steffan ; C. Hennesthal ; S.H. Heinemann |
| Voltage-Dependent Ion Channels: Analysis of Nonideal Macroscopic Current Data / S.H. Chung ; P.W. Gage |
| Signal Processing Techniques for Channel Current Analysis Based on Hidden Markov Models / B.S. Rothberg ; K.L. Magleby |
| Investigating Single-Channel Gating Mechanisms through Analysis of Two-Dimensional Dwell-Time Distributions |
| Expression Systems / K. Radford ; G. Buell |
| Expression of Ligand-Gated Ion Channels Using Semliki Forest Virus and Baculovirus / M.U. Ehrengruber ; M. Lanzrein ; Y. Xu ; M.C. Jasek ; D.B. Kantor ; E.M. Schuman ; H.A. Lester ; N. Davidson |
| Recombinant Adenovirus-Mediated Expression in Nervous System of Genes Coding for Ion Channels and Other Molecules Involved in Synaptic Function / M.W. Nowak ; J.P. Gallivan ; S.K. Silverman ; C.G. Labarca ; D.A. Dougherty |
| In Vivo Incorporation of Unnatural Amino Acids into Ion Channels in Xenopus Oocyte Expression System / T.M. Shih ; R.D. Smith ; L. Toro ; A.L. Goldin |
| High-Level Expression and Detection of Ion Channels in Xenopus Oocytes |
| Contributors |
| Preface |
| Volumes in Series |
| Modern Applications of Electrophysiological Techniques: Patch Clamp Techniques: An Overview / M. Cahalan ; E. NeherSection I： |
| Constructing a Patch Clamp Setup |
| Glass Technology for Patch Clamp Electrodes |
| Ion Channel Selectivity, Permeation, and Block |
| Access Resistance and Space Clamp Problems Associated with Whole-Cell Patch Clamping / C.M. Armstrong ; W.F. Gilly |
| Correction for Liquid Junction Potentials in Patch Clamp Experiments |
| Nonstationary Noise Analysis and Application to Patch Clamp Recordings / F. Conti |
| Prevention of Rundown in Electrophysiological Recording / S.J. Korn |
| Patch Voltage Clamping with Low-Resistance Seals: Loose Patch Clamp / W.M. Roberts ; W. Almers |
| Perfusing Patch Pipettes / J.M. Tang ; J. Wang ; R.S. Eisenberg |
| Whole-Cell Recording of Calcium Channel Currents / B.P. Bean |
| Recording from Calcium-Activated Potassium Channels / B.S. Pallotta ; A.L. Blatz |
| Patch-Clamping Cells in Sliced Tissue Preparations / F.A. Edwards ; A. Konnerth |
| Expression of Ion Channels / Section II： |
| Expression of Ion Channels in Xenopus Oocytes: Xenopus Oocyte Microinjection: From Gene to Protein / H. Soreq ; S. SeidmanA： |
| Maintenance of Xenopus laevis and Oocyte Injection |
| Preparation of RNA for Injection into Xenopus Oocytes / K. Sumikawa |
| Tissue RNA as Source of Ion Channels and Receptors / T.P. Snutch ; G. Mandel |
| In Vitro Synthesis of RNA for Expression of Ion Channels in Xenopus Oocytes / T. Snutch |
| Electrophysiological Recording from Xenopus Oocytes / W. Stühmer |
| Use of Stage II-III Xenopus Oocytes to Study Voltage-Dependent Ion Channels / D.S. Krafte |
| Intracellular Perfusion of Xenopus Oocytes / N. Dascal ; G. Chilcott |
| Recording of Gating Currents from Xenopus Oocytes and Gating Noise Analysis |
| Ligand-Binding Assays in Xenopus Oocytes / A.L. Buller ; M.M. White |
| Expression of Gap Junctional Proteins in Xenopus Oocyte Pairs / L. Ebihara |
| Regulation of Intracellular Calcium Activity in Xenopus Oocytes / Y. Oron |
| Expression of Ion Channels Using Other Systems: Stable Expression of Heterologous Multisubunit Protein Complexes Established by Calcium Phosphate- or Lipid-Mediated Cotransfection / T. ClaudioB： |
| Vaccinia Virus as Vector to Express Ion Channel Genes / A. Karschin ; B.A. Thorne ; G. Thomas |
| Expression of Ion Channels in Cultured Cells Using Baculovirus / A. Kamb ; J.I. Korenbrot ; J. Kitajewski |
| Probing Molecular Structure and Structural Changes of Voltage-Gated Channel by Expressing Mutant Channels in Yeast and Reconstituting Them into Planar Membranes / M. Colombini ; S. Peng ; E. Blachly-Dyson ; M. Forte |
| Reconstitution of Ion Channels in Lipid Bilayers: Insertion of Ion Channels into Pla / Section III： |
| Contributors to Volume 294 |
| Physical Methods: Optical Measurements of Calcium Signals in Mammalian Presynaptic Terminals / P. Saggau ; R. Gray ; J.A. Dani |
| Fluorescent Techniques for Measuring Ion Channel Activity / G. Velicelebi ; K.A. Strauderman ; M.A. Varney ; M. Akong ; S.D. Hess ; E.C. Johnson |
| Tagging Potassium Ion Channels with Green Fluorescent Protein to Study Mobility and Interactions with Other Proteins / E.S. Levitan |
| Spin Label Electron Spin Resonance and Fourier Transform Spectroscopy for Structural/Dynamic Measurements on Ion Channels / D. Marsh |
| Nuclear Magnetic Resonance Spectroscopy of Peptide Ion Channel Ligands: Cloning and Expression as Aid to Evaluation of Structural and Dynamic Properties / M.D. Reily ; A.M. Bokman ; J. Offord ; P. McConnell |
| Ligand Binding Methods for Analysis of Ion Channel Structure and Function / S.E. Pedersen ; M.M. Lurtz ; R.V.L. Papineni |
| Three-Dimensional Structure of Membrane Proteins Determined by Two-Dimensional Crystallization, Electron Cryomicroscopy, and Image Analysis / M. Yeager ; V.M. Unger ; A.K. Mitra |
| Drug-Dependent Ion Channel Gating by Application of Concentration Jumps Using U-Tube Technique / F. Bretschneider ; F. Markwardt |
| Voltage Clamp Biosensors for Capillary Electrophoresis / O. Orwar ; K. Jardemark ; C. Farre ; I. Jacobson ; A. Moscho ; J.B. Shear ; H.A. Fishman ; S.J. Lillard ; R.N. Zare |
| Ion Channels as Tools to Monitor Lipid Bilayer-Membrane Protein Interactions: Gramicidin Channels as Molecular Force Transducers / O.S. Andersen ; C. Nielsen ; A.M. Maer ; J.A. Lundbaek ; M. Goulian ; R.E. Koeppe, III |
| Purification and Reconstitution: Purification and Reconstitution of Epithelial Chloride Channel Cystic Fibrosis Transmembrane Conductance Regulator / M. Ramjeesingh ; E. Garami ; L.-J. Huan ; K. Galley ; C. Li ; Y. Wang ; C.E. Bear |
| Purification, Characterization, and Reconstitution of Cyclic Nucleotide-Gated Channels / R.S. Molday ; L.L. Molday |
| Purification and Heterologous Expression of Inhibitory Glycine Receptors / B. Laube ; H. Betz |
| Purification and Functional Reconstitution of High-Conductance Calcium-Activated Potassium Channel from Smooth Muscle / M.L. Garcia ; K.M. Giangiacomo ; M. Hanner ; H.-G. Knaus ; O.B. McManus ; W.A. Schmalhofer ; G.J. Kaczorowski |
| Reconstitution of Native and Cloned Channels into Planar Bilayers / I. Favre ; Y.-M. Sun ; E. Moczydlowski |
| Iodide Channel of the Thyroid: Reconstituting Ion Channels into Planar Lipid Bilayers / P.E. Golstein ; A. Sener ; F. Colin ; R. Beauwens |
| Nystatin/Ergosterol Method for Reconstituting Ion Channels into Planar Lipid Bilayers / D.J. Woodbury |
| Isolation of Transport Vesicles That Deliver Ion Channels to the Cell Surface / S. Sattsangi ; W.F. Wonderlin |
| Second Messengers and Biochemical Approaches: Protein Phosphorylation of Ligand-Gated Ion Channels / A.L. Mammen ; S. Kamboj ; R.L. Huganir |
| Analysis of Ion Channel Associated Proteins / M. Wyszynski |
| Signal Transduction through Ion Channels Associated with Excitatory Amino Acid Receptors / K. Ogita ; Y. Yoneda |
| Secondary Messenger Regulation of Ion Channels/Plant Patch Clamping / S.M. Assmann ; L. Romano |
| Special Channels: ATP-Sensitive Potassium Channels / M. Schwanstecher ; C. Schwanstecher ; F. Chudziak ; U. Panten ; J.P. Clement IV ; G. Gonzalez ; L. Aguilar-Bryan ; J. Bryan |
| Mechanosensitive Channels |
| Modern Applications of Electrophysiological Techniques |
| Patch Clamp Techniques: An Overview |
| Expression of Ion Channels in Xenopus Oocytes: Xenopus |
| Oocyte Microinjection: From Gene to Protein |
| Expression of Ion Channels Using Other Systems |
| Stable Expression of Heterologous Multisubunit Protein Complexes Established by Calcium Phosphate- or Lipid-Mediated Cotransfection |
| Reconstitution of Ion Channels in Lipid Bilayers |
| Insertion of Ion Channels into Planar Lipid Bilayers by Vesicle Fusion / P. Labarca ; R. Latorre |
| Planar Lipid Bilayers on Patch Pipettes: Bilayer Formation and Ion Channel Incorporation / B.E. Ehrlich |
| Surface Charge Effects on Ion Conduction in Ion Channels / D. Naranjo |
| Determination of Ion Permeability by Fluorescence Quenching / A.M. Garcia |
| Synthetic Peptides and Proteins as Models for Pore-Forming Structure of Channel Proteins / A. Grove ; T. Iwamoto ; M.S. Montal ; J.M. Tomich ; M. Montal |
| Purification of Ion Channel Proteins and Genes |
| Purification and Reconstitution of Skeletal Muscle Calcium Channels / V. Florio ; J. Striessnig ; W.A. Catterall |
| Purification and Reconstitution of Nicotinic Acetylcholine Receptor / A. Chak |
| Purification, Affinity Labeling, and Reconstitution of a Voltage-Sensitive Potassium Channels / H. Rehm ; M. Lazudunski |
| Affinity Purification and Reconstitution of Calcium-Activated Potassium Channels / D.A. Klaerke ; P.L. J#248;rgensen |
| Assay and Purification of Neuronal Receptors for Inositol 1,4,5-Triphosphate / S.R. Hingorani ; W.S. Agnew |
| Isolation of Ion Channel Genes by Expression Cloning in Xenopus Oocytes / G.C. Frech ; R.H. Joho |
| Hybrid Arrest Technique to Test for Functional Roles of Cloned cDNAs and to Identify Homologies among Ion Channel Genes / I. Lotan |
| Cloning of Ion Channel Gene Families Using the Polymerase Chain Reaction / E. Vega-Saenz De Miera ; J.-W. Lin |
| Overview of Toxins and Drugs as Tools to Study Excitable Membrane Ion Channels I: Voltage-Activated Channels / T. Narahashi ; M.D. Herman |
| Overview of Toxins and Drugs as Tools to Study Excitable Membrane Ion Channels II: Transmitter-Activated Channels |
| Biosynthesis of Ion Channels in Cell-Free and Metabolically Labeled Cell Systems / W.B. Thornhill ; S.R. Levinson |
| Recording of Ion Channels of Cellular Organelles and Microorganisms |
| Patch Clamp Techniques in Study of Ion Channels from Organelles / B.U. Keller ; R. Hedrich |
| Patch Clamp Studies of Microbial Ion Channels / Y. Saimi ; B. Martinac ; A.H. Delcour ; P.V. Minorsky ; M.C. Gustin ; M.R. Culbertson ; J. Adler ; C. Kung |
| Studies on Intact Sarcoplasmic Reticulum: Patch Clamp Recording and Tension Measurement in Lobster Split Muscle Fibers |
| Planar Bilayer Recording of Ryanodine Receptors of Sarcoplasmic Reticulum / R. Coronado ; S. Kawano ; C.J. Lee ; C. Valdivia ; H.H. Valdivia |
| Data Storage and Analysis |
| Software for Acquisition and Analysis of Ion Channel Data: Choices, Tasks, and Strategies / R.J. French |
| Stationary Single-Channel Analysis / M.B. Jackson |
| Analysis of Nonstationary Single-Channel Currents / F.J. Sigworth ; J. Zhou |
| Preventing Artifacts and Reducing Errors in Single-Channel Analysis |
| Analysis of Drug Action at Single-Channel Level |
| Analysis of Sodium Channel Tail Currents / G. Cota |
| Calculation of Ion Currents from Energy Profiles and Energy Profiles from Ion Currents in a Multibarrier, Multisite, Multioccupancy Channel Model / O. Alvarez ; A. Villarroel ; G. Eisenman |
| Author Index |
| Subject Index |
| Assembly / J. Xu ; M. Li |
| Methods Used to Study Subunit Assembly of Potassium Channels / Z.F. Sheng ; C. Deutsch |
| Assembly of Ion Channels / J.S. Trimmer |
| Analysis of K + Channel Biosynthesis and Assembly in Transfected Mammalian Cells |
| Genetics / T.M. Ishii ; P. Zerr ; X-m. Xia ; C.T. Bond ; J. Maylie ; J.P. Adelman |
| Site-Directed Mutagenesis / S. Richard ; P. Lory ; E. Bourinet ; J. Nargeot |
