Expression, Purification, and Posttranslational Modification / S.L. Campbell-Burk ; J.W. Carpenter |
Refolding and Purification of Ras Proteins / E. Porfiri ; T. Evans ; G.E. Bollag ; R. Clark ; J.F. Hancock |
Purification of Baculovirus-Expressed Recombinant Ras and Rap Proteins / F. McCormick |
Purification of Recombinant Ras GTPase-Activating Proteins / B. Rubinfeld ; P. Polakis |
Purification of Baculovirus-Produced Rap 1 GTPase-Activating Proteins / G.L. James ; M.S. Brown ; J.L. Goldstein |
Assays for Inhibitors for CAAX Farnesyltransferase in Vitro and in Intact Cells / R. Khosravi-Far ; C.J. Der |
Prenylation Analysis of Bacterially Expressed and Insect Cell-Expressed Ras and Ras-Related Proteins |
Reticulocyte Lysate Assay for in Vitro Translation and Posttranslational Modification of Ras Proteins / C. Volker ; J.B. Stock |
Carboxyl Methylation of Ras-Related Proteins / F. Tamanoi ; H. Mitsuzawa |
Use of Yeast for Identification of Farnesyltransferase Inhibitors and for Generation of Mutant Farnesyltransferases |
Guanine Nucleotide Exchange and Hydrolysis / C. Lenzen ; R.H. Cool ; A. Wittinghofer |
Analysis of Intrinsic and CDC25-Stimulated Guanine Nucleotide Exchange of p21 ras --Nucleotide Complexes by Fluorescence Measurements / J. Downward |
Measurement of Nucleotide Exchange and Hydrolysis Activities in Immunoprecipitates / J.B. Gibbs |
Determination of Guanine Nucleotides Bound to Ras in Mammalian Cells / M. Frech ; D. Cussac ; P. Chardin ; D. Bar-Sagi |
Purification of Baculovirus-Expressed Human Sos1 Protein / M. Hart ; S. Powers |
Ras-Cdc25 and Rho-Dbl Binding Assays: Complex Formation in Vitro / R.D. Mosteller ; W. Park ; D. Broek |
Analysis of Interaction between Ras and CDC25 Guanine Nucleotide Exchange Factor Using Yeast GAL4 Two-Hybrid System / T. Satoh ; Y. Kaziro |
Measurement of Ras-Bound Guanine Nucleotide in Stimulated Hematopoietic Cells / A.M.M. de Vries-Smits ; L. van der Voorn ; J.L. Bos |
Measurements of GTP/GDP Exchange in Permeabilized Fibroblasts |
Intrinsic and GTPase-Activating Protein-Stimulated Ras GTPase Assays / M.D. Schaber |
Determination of Ras and GTPase-Activating Protein Interactions by Kinetic Competition Assay / C. Ellis ; V. Measday ; M.F. Moran |
Phosphorylation-Dependent Complexes of p120 Ras-Specific GTPase-Activating Proteins with p62 and p190 |
Cell Expression and Analysis: in Vitro / A.D. Cox ; P.A. Solski ; J.D. Jordan |
Analysis of Ras Protein Expression in Mammalian Cells / A.M.M. deVries-Smits ; B.M.T. Burgering |
Vaccinia Virus Expression of p21ras Asn-17 / H. Cai ; G.M. Cooper |
Inducible Expression of Ras N17 Dominant Inhibitory Protein |
Prenylation and Palmitoylation Analysis / C.W.M. Reuter ; A.D. Catling ; M.J. Weber |
Immune Complex Kinase Assays for Mitogen-Activated Protein Kinase and MEK / S. Kuroda ; K. Shimizu ; B. Yamamori ; Y. Takai |
Cell-Free Assay System for Ras-Dependent MEK Activation / P. Dent ; G. Romero ; D. Castle ; T.W. Sturgill |
Preparation and Use of Semiintact Mammalian Cells for Analysis of Signal Transduction / C.J |
Expression, Purification, and Posttranslational Modification / S.L. Campbell-Burk ; J.W. Carpenter |
Refolding and Purification of Ras Proteins / E. Porfiri ; T. Evans ; G.E. Bollag ; R. Clark ; J.F. Hancock |
Purification of Baculovirus-Expressed Recombinant Ras and Rap Proteins / F. McCormick |
Purification of Recombinant Ras GTPase-Activating Proteins / B. Rubinfeld ; P. Polakis |
Purification of Baculovirus-Produced Rap 1 GTPase-Activating Proteins / G.L. James ; M.S. Brown ; J.L. Goldstein |
Assays for Inhibitors for CAAX Farnesyltransferase in Vitro and in Intact Cells / R. Khosravi-Far ; C.J. Der |