DNA isolation and sequencing

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DNA isolation and sequencing / Bruce A. Roe, Judy S. Crabtree and Akbar S. Khan

資料種別:

図書

出版情報:

New York : John Wiley & Sons, 1996

形態:

163 p.(loose-leaf form) ; 13 × 22 cm

シリーズ名:

Essential techniques series <BA2686266X>

著者名:

ISBN:

9780471963240 [0471963240] (:alk. paper)

書誌ID:

BA27648472

子書誌情報

フルテキスト

所蔵情報

他の版・巻

書誌詳細

注記:

Includes bibliographical references and index

主題:

DNA--Separation--Methodology; Nucleotide sequence--Methodology

目次情報:

Partial table of contents

General Methods

Restriction Digestion

Agarose Gel Electrophoresis

Bacterial Cell Maintenance and Storage

Random Subclone Generation

Sonication

DNA Ligation

Methods for DNA Isolation

Large Scale Double-Stranded DNA Isolation

Miniprep Double-Stranded DNA Isolation

Methods for DNA Sequencing

Radiolabeled Sequencing Gel Preparation, Loading and Electrophoresis

Flourescent DNA Sequencing

Additional Methods

Polymerase Chain Reaction (PCR)

Purification of PCR Fragments for Cloning

Synthesis and Purification of Oligonucleotides

Appendices

Index

Partial table of contents

General Methods

Restriction Digestion

Agarose Gel Electrophoresis

Bacterial Cell Maintenance and Storage

Random Subclone Generation

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