Contributors |
Preface |
Local Probe Techniques / J. K. Heinrich Horber1.: |
Introduction / I.: |
Scanning Tunneling Microscopy / II.: |
Atomic Force Microscopy / III.: |
Force Spectroscopy / IV.: |
Photonic Force Microscopy / V.: |
References |
The Atomic Force Microscope in the Study of Membrane Fusion and Exocytosis / Bhanu P. Jena ; Sang-Joon Cho2.: |
Methods |
AFM Studies on Live Cells |
Identification of New Plasma Membrane Structures Involved in Exocytosis |
Future of AFM in the Study of Live Cells |
Atomic Force Microscope Imaging of Cells and Membranes / Eric Lesniewska ; Pierre Emmanuel Milhiet ; Marie-Cecile Giocondi ; Christian Le Grimellec3.: |
AFM Equipment |
AFM Operating Modes |
Requirements for the Imaging of Intact Cells |
Imaging of Cells |
Imaging of Isolated Membranes / VI.: |
Conclusion and Perspectives / VII.: |
Measuring the Elastic Properties of Living Cells by the Atomic Force Microscope / Manfred Radmacher4.: |
Principles of Measurement |
Application to Cells |
Mechanics of Cellular Dynamics |
Summary |
Cell Adhesion Measured by Force Spectroscopy on Living Cells / Martin Benoit5.: |
Instrumentation |
Preparations of the Force Sensor for Measurements with Living Cells |
Cell Culture |
Final Remarks |
Molecular Recognition Studies Using the Atomic Force Microscope / Peter Hinterdorfer6.: |
Experimental Approach |
Dynamic Force Spectroscopy |
Recognition Imaging |
The Biophysics of Sensory Cells of the Inner Ear Examined by Atomic Force Microscopy and Patch Clamp / Matthias G. Langer ; Assen Koitschev7.: |
Morphology and Function of Cochlear Hair Cells |
AFM Technology |
Applications |
Discussion |
Outlook |
Biotechnological Applications of Atomic Force Microscopy / Guillaume Charras ; Petri Lehenkari ; Mike Horton8.: |
Analysis |
Application Examples |
Future Directions and Improvements |
Cellular Membranes Studied by Photonic Force Microscopy / Arnd Pralle ; Ernst-Ludwig Florin9.: |
Experimental Considerations |
Methods for Biological Probe Microscopy in Aqueous Fluids / Johannes H. Kindt ; John C. Sitko ; Lia I. Pietrasanta ; Emin Oroudjev ; Nathan Becker ; Mario B. Viani ; Helen G. Hansma10.: |
Substrates/Surfaces |
Basic Methods for Atomic Force Microscopy in Aqueous Fluids |
Molecular Force Probing |
Advanced Fluid Handling |
Conclusion |
Supported Lipid Bilayers as Effective Substrates for Atomic Force Microscopy / Daniel M. Czajkowsky ; Zhifeng Shao11.: |
Preparation of the Supported Bilayer Substrates |
Examples of Applications |
Cryo-Atomic Force Microscopy / Sitong Sheng12.: |
Designs and Instrumentation |
Applications in Structural Biology |
Deep Etching as the Preferred Sample Preparation Method |
New Directions |
Conformations, Flexibility, and Interactions Observed on Individual Membrane Proteins by Atomic Force Microscopy / Daniel J. Muller ; Andreas Engel13.: |
High-Resolution AFM Imaging |
Identification of Membrane Proteins |
Observing the Oligomerization of Membrane Proteins |
Unraveling the Conformational Variability of Membrane Proteins |
Comparing AFM Topographs to Atomic Models |
Conformational Changes of Native Membrane Proteins |
Observing the Assembly of Membrane Proteins / VIII.: |
Detecting Intra- and Intermolecular Forces of Proteins / IX.: |
Conclusions and Perspectives / X.: |
Single-Molecule Force Measurements / Aileen Chen ; Vincent T. Moy14.: |
Experimental Design |
Forced Unfolding of Single Proteins / S. M. Altmann ; P.-F. Lenne15.: |
The Biological System |
Forced Unfolding |
Models |
Conclusions and Prospects |
Appendices |
Developments in Dynamic Force Microscopy and Spectroscopy / A. D. L. Humphris ; M. J. Miles16.: |
Active Q Control |
Application of Active Q-Control AFM |
Transverse Dynamic Force Techniques |
Conclusions |
Scanning Force Microscopy Studies on the Structure and Dynamics of Single DNA Molecules / Giampaolo Zuccheri ; Bruno Samori17.: |
The Control of Adsorption of DNA on Surfaces |
Air Imaging of DNA: Which Present, Which Future? |
Imaging DNA in Fluid |
DNA Manipulation with the SFM: The Controlled Dissection of DNA |
The Study of DNA Conformations and Mechanics: Curvature and Flexibility |
An Interesting Issue: The Shape of Supercoiled DNA |
Index |
Volumes in Series |
Contributors |
Preface |
Local Probe Techniques / J. K. Heinrich Horber1.: |
Introduction / I.: |
Scanning Tunneling Microscopy / II.: |
Atomic Force Microscopy / III.: |