Foreword |
Preface |
Introduction and Future of Site-Directed Spin Labeling of Membrane Proteins / Marcus A. HemmingaChapter 1: |
Structural Biology and Proteomics / 1: |
Membrane Proteins: Production and Reconstitution Challenges / 2: |
SDSL-ESR / 3: |
Cysteine Modification / 4: |
Structure and Dynamics Information from SDSL-ESR / 5: |
Pulsed ESR Spectroscopy / 6: |
High-Field ESR Spectroscopy / 7: |
Molecular Dynamics Simulations / 8: |
Spectral Simulation and Analysis / 9: |
Comparison with Site-Directed Fluorescence Labeling / 10: |
Future / 11: |
Instrumentation and Experimental Setup / Gunnar JeschkeChapter 2: |
Continuous-Wave ESR |
Basics of Pulsed ESR |
Pulsed ENDOR |
Pulsed ELDOR (DEER) |
Acknowledgments |
Problems |
Answers |
Advanced ESR Spectroscopy in Membrane Biophysics / Janez StrancarChapter 3: |
Introduction |
Motional Averaging in a Spin-Labeled Biomembrane |
Strategies for Calculating Powder Spectra |
Solving an Inverse Problem and Condensation of Results |
Appendix |
Practical Pulsed Dipolar ESR (DEER) / Piotr G. Fajer ; Louise Brown ; Likai SongChapter 4: |
DEER Signal |
Practical DEER |
Applications |
Membrane Protein Structure and Dynamics Studied by Site-Directed Spin-Labeling ESR / Enrica Bordignon ; Heinz-Jurgen SteinhoffChapter 5: |
Spin Labeling |
Structural Information Derived from ESR Spectra Analysis |
Detection of Conformational Changes |
High-Field ESR Spectroscopy in Membrane and Protein Biophysics / Tatyana I. Smirnova ; Alex I. SmirnovChapter 6: |
Analysis of High-Field ESR Spectra of Spin Labels |
High-Field ESR of Spin-Labeled Aqueous Samples: Experimental Considerations |
High-field ESR in Studies of Molecular Dynamics |
High-field ESR in Studies of Molecular Structure |
Characterization of the Nitroxide Microenvironment by High-Field ESR |
Perspectives |
Appendices: Software Descriptions |
Molecular Modeling of Spin Labels / Mikolai I. Fajer ; Kenneth L. SaleAppendix 1: |
System Requirements |
Preparation of Structures |
Simulations |
Analysis of Nitroxide Trajectories |
Force Fields |
Spin Label Topologies |
SIMPOW6: A Software Package for the Simulation of ESR Powder-Type Spectra / Mark J. Nilges ; Karen Mattson ; R. Linn BelfordAppendix 2: |
Spin Hamiltonian for SIMP0W6 |
Calculation of Resonance Fields |
Lineshapes |
Intensity (Transition Moment) |
Generation (Integration) of a Powder Spectrum |
Spectral Optimization |
Summary of Input Parameters |
Running the Program |
Examples |
Appendix A: Format of the Input Files: Spin Hamiltonian Parameters |
Appendix B: Format of the Input Files: Optimization Parameters and Control / 12: |
Appendix C: Format of the Output Simulation Files / 13: |
ACERT Software: Simulation and Analysis of ESR Spectra / Jack H. FreedAppendix 3: |
DeerAnalysis 2006: Distance Measurements on Nanoscopic Length Scales by Pulse ESR / Appendix 4: |
EWVoigt and EWVoigtn: Inhomogeneous Line Shape Simulation and Fitting Programs / Appendix 5: |
Convolution-Based Fitting of Continuous Wave EPR Spectra |
Brief Description of EWVOIGT Capabilities |
Convolution Algorithm with Levenberg-Marquardt Optimization for Fitting Inhomogeneous EPR Spectra |
EasySpin: Simulating cw ESR Spectra / Stefan Stoll ; Arthur SchweigerAppendix 6: |
Four Dynamic Regimes in cw ESR |
Simulation of cw ESR Spectra |
Other EasySpin functions |
EPRSIM-C: A Spectral Analysis Package / Appendix 7: |
Main Characteristics |
EPRSIM-C Library |
EPRSIM-C Programs |
Contents of Previous Volumes |
Index |
Foreword |
Preface |
Introduction and Future of Site-Directed Spin Labeling of Membrane Proteins / Marcus A. HemmingaChapter 1: |
Structural Biology and Proteomics / 1: |
Membrane Proteins: Production and Reconstitution Challenges / 2: |
SDSL-ESR / 3: |