RNA modification

所蔵情報QRコード

資料種別:: 図書
出版情報:: Amsterdam ; Tokyo : Academic Press, c2007
形態:: xlii, 433 p., [8] p. of plates ; 24 cm
シリーズ名:: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 425 <BA00197543>
著者名:: Gott, Jonatha M <DA15947370>
ISBN:: 9780123741554 [0123741556]
書誌ID:: BA82765058

子書誌情報

フルテキスト

所蔵情報

他の版・巻

書誌詳細

注記:

Includes bibliographical references and indexes

言語:

英語

目次情報:

Modified Nucleotides / Section I：

Identifying modifications in RNA by mass spectrometry / Chapter 1：

Identification of modified residues in RNAs by reverse transcription-based methods. / Chapter 2：

tRNA Modifications / Section II：

Detection of enzymatic activity of transfer RNA modification enzymes using radiolabelled tRNA substrates / Chapter 3：

In vitro detection of the enzymatic activity of folate-dependent tRNA(U54, C5)-methyltransferase / Chapter 4：

Probing the intermediacy of covalent RNAenzyme complexes in RNA modification enzymes / Chapter 5：

Identification and characterization of modification enzymes by biochemical analysis of the proteome / Chapter 6：

Identification of genes encoding tRNA modification enzymes via comparative genomics / Chapter 7：

Identification and characterization of archaeal and fungal tRNA methyltransferases / Chapter 8：

Mass spectrometric identification and characterization of RNA-modifying enzymes / Chapter 9：

Chaplet column chromatography: isolation of a large set of individual RNAs in a single step. / Chapter 10：

SNO-mediated Modifications / Section III：

Biochemical Purification of box H/ACA RNPs involved in pseudouridylation / Chapter 11：

In Vitro Reconstitution and Affinity Purification of Catalytically Active Archaeal Box C/D sRNP Complexes / Chapter 12：

Identifying effects of snoRNA-guided modifications on the synthesis and function of the yeast ribosome / Chapter 13：

The U1 snRNA hairpin II as a RNA affinity tag for selecting snoRNP complexes / Chapter 14：

A Dedicated Computational Approach for the Identification of Archaeal H/ACA sRNAs / Chapter 15：

Reconstitution of archaeal H/ACA sRNPs and test of their activity / Chapter 16：