Guide to protein purification

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Guide to protein purification / edited by Richard R. Burgess, Murray P. Deutscher

版:

2nd ed

資料種別:

図書

出版情報:

Amsterdam ; Tokyo : Academic Press, 2009

形態:

liii, 851 p., [8] p. of plates ; 24 cm

シリーズ名:

Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 463 <BA00197543>

著者名:

ISBN:

9780123745361 [0123745365] (: hardback)
9780123749789 [0123749786] (: pbk)

書誌ID:

BB00142607

子書誌情報

フルテキスト

所蔵情報

他の版・巻

書誌詳細

注記:

Includes bibliographical references and indexes

主題:

Proteins--Purification

言語:

英語

目次情報:

Why Purify Enzymes? / Murray Deutscher ; Arthur Kornberg

Developing Purification Procedures / Section I：

Strategies and Considerations for Protein Purifications / Stuart Linn

Use of Bioinformatics in Planning a Protein Purification / Richard R. Burgess

Preparing A Purification Summary Table

General Methods for Handling Proteins and Enzymes / Section II：

Setting Up A Laboratory / Murray P. Deutscher

Buffers: Principle and Practice / Vincent S. Stoll ; John S. Blanchard

Measurement of Enzyme Activity / T. K. Harris ; M. M. Keshwani

Quantitation of Protein / James E. Noble ; Marc J. A. Bailey

Concentration of Proteins and Removal of Solutes / Jonathan K. Romero ; David R. H. Evans ; Matthew Westoby

Maintaining Protein Stability

Recombinant Protein Expression and Purification / Section III：

Selecting an Appropriate Method for Expressing a Recombinant Protein / William H. Brondyk

Bacterial Systems for Production of Heterologous Proteins / Frank R. Collart ; Ashley M. Frank ; Sarah Zerbs

Expression in the Yeast Pichia Pastoris / James M. Cregg ; Ilya Tolstorukov ; Anasua Kusari ; Jay Sunga ; Knut Madden ; Thomas Chappell

Baculovirus-Insect Cell Expression Systems / Donald L. Jarvis

Recombinant Protein Production By Transient Gene Transfer Into Mammalian Cells / Sabine Geisse ; Cornelia Fux

Tagging For Protein Expression / Arun Malhotra

Refolding of Solubilized Inclusion Body Proteins

Preparation of Extracts and Subcellular Fractionation / Section IV：

Advances In Preparation of Biological Extracts for Protein Purification / Anthony C. Grabski

Isolation of Subcellular Organelles and Structures / Jörg Von Hagen ; Uwe Michelsen

Purification Procedures: Bulk Methods / Section V：

Protein Precipitation Techniques

Affi-Gel Blue for Nucleic Acid Removal and Early Enrichment of Nucleotide Binding Proteins

Purification Procedures: Chromatographic Methods / Section VI：

Ion Exchange Chromatography / Alois Jungbauer ; Rainer Hahn

Gel Filtration / Earle Stellwagen

Protein Chromatography on Hydroxyapatite Columns / Larry J. Cummings ; Mark A. Snyder ; Kimberly Brisack

Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification Applications / Justin T. Mccue

Purification Procedures: Affininty Methods / Section VII：

Affinity Chromatography: General Methods / Marjeta Urh ; Dan Simpson ; Kate Zhao

Immobilized-Metal Affinity Chromatography (IMAC) / Frank Schäfer* Helena Block ; Barbara Maertens ; Anne Spriestersbach ; Nicole Brinker ; Jan Kubicek ; Roland Fabis And Jörg Labahn

Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for Immunoaffinity Chromatography / Nancy E. Thompson ; Katherine M. Foley ; Elizabeth S. Stalder ; And Richard R. Burgess

Purification Procedures: Electrophoretic Methods / Section VIII：

One-Dimensional Gel Electrophoresis / David E. Garfin

Protein Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis and Isoelectric Focusing / Friedman

Protein Gel Staining Methods: An Introduction And Overview / Thomas H. Steinberg

Elution of Proteins From Gels

Immunodetection By Protein Blotting Performing and Optimizing Western Blots With An Emphasis on Chemiluminescent Detection / Alice Alegria-Schaffer ; Krishna Vattem ; Andrew Lodge

Purification Procedures: Membrane Proteins And Glycoproteins / Section IX：

Detergents: An Overview / Dirk Linke

Purification of Membrane Proteins / Sue-Hwa Lin ; Guido Guidotti

Purification of Recombinant G-Protein-Coupled Receptors / Reinhard Grisshammer

Cell-Free Translation Of Integral Membrane Proteins Into Unilamelar Liposomes / Michael A. Goren ; Akira Nozawa ; Shin-Ichi Makino ; Russell L. Wrobel ; Brian G. Fox

Characterization of Purified Proteins / Section X：

Determination of Protein Purity / David G. Rhodes ; Thomas M. Laue

Determination of Size, Molecular Weight, and Presence of Subunits / Robert E. Bossio

Identification and Quantification of Protein Posttranslational Modifications / Andrew Link

Parallel Methods For Expression And Purification / Scott A. Lesley

Techniques To Isolate O2-Sensitive Proteins: [4Fe-4S]-FNR As An Example / Aixin Yan ; Patricia J. Kiley

Rethinking Your Purification Procedure

Important But Little Known (Or Forgotten) Artifacts in Protein Biochemistry

Bioinformatics in Planning a Protein Purification / Linn ; Burgess

Preparing a Protein Purification Summary Table

Setting up a Laboratory / Deutscher

Strategy for Choosing and Expression System / Blanchard ; Harris ; Bailey ; Noble ; Brondyk

Bacterial Expression Systems / Collart

Yeast Expression Systems / Cregg

Mammalian Expression Systems / Jarvis ; Geisse ; Knopf

Expression of Tagged Proteins / Melhorta

Re-folding of Solubilized Inclusion Body Proetin

Preparation of Biological Extract / Grabski

Why Purify Enzymes? / Murray Deutscher ; Arthur Kornberg

Developing Purification Procedures / Section I：

Strategies and Considerations for Protein Purifications / Stuart Linn

Use of Bioinformatics in Planning a Protein Purification / Richard R. Burgess

Preparing A Purification Summary Table

General Methods for Handling Proteins and Enzymes / Section II：

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