| Contributors |
| Volumes in Series |
| Protein 3-Nitrotyrosine in Complex Biological Samples: Quantification by High-Pressure Liquid Chromatography/Electrochemical Detection and Emergence of Proteomic Approaches for Unbiased Identification of Modification Sites / Tal Nuriel ; Ruba S. Deeb ; David P. Hajjar ; Steven S. Gross1： |
| Introduction |
| Quantification of 3-NT in Proteins Using HPLC Separation and Electrochemical (EC) Detection / 2： |
| Protocol for Quantification of 3-NT in Hydrolyzed Proteins Using HPLC-EC / 3： |
| Beyond Quantification: Specification of 3-NT Sites in Proteins / 4： |
| Acknowledgment |
| References |
| Selective Fluorogenic Derivatization of 3-Nitrotyrosine and 3,4-Dihydroxyphenylalanine in Peptides: A Method Designed for Quantitative Proteomic Analysis / Victor S. Sharov ; Elena S. Dremina ; Justin Pennington ; Jacque Killmer ; Christopher Asmus ; Maria Thorson ; Sung Jung Hong ; Xiaobao Li ; John F. Stobaugh ; Christian Schoneich |
| Design of a Fluorogenic Derivatization Method |
| Model Studies with 4-Methylcatechol and 2-Aminocresol |
| Derivatization of a 3-Nitrotyrosine-Containing Model Peptide with Benzylamine and 4-Aminomethylbenzene Sulfonic Acid |
| Conclusions / 5： |
| Nitroalkenes: Synthesis, Characterization, and Effects on Macrophage Activation / Ana Maria Ferreira ; Andres Trostchansky ; Mariana Ferrari ; Jose M. Souza ; Homero Rubbo |
| Synthesis and Characterization of Cholesteryl-Nitrolinoleate |
| Synthesis and Identification of Nitroalkenes Isomers: Mass Spectrometry Analysis of Nitroarachidonate |
| Determination of Lipid Nitration during Macrophage Activation |
| Effects of Nitroalkenes on NOS2 Induction by Activated Macrophages |
| Analysis of HO-1 Induction and NF-[kappa]B Activation / 6： |
| Acknowledgments |
| In-Gel Detection of S-Nitrosated Proteins Using Fluorescence Methods / Nicholas J. Kettenhofen ; Xunde Wang ; Mark T. Gladwin ; Neil Hogg |
| Role of Trace Metal Ions in Ascorbate-Mediated Reduction of S-Nitrosothiols |
| Detection of Protein S-Nitrosation Using Fluorescent Labeling Methods |
| Current Protocol for CyDye-Switch Method |
| Difference Gel Electrophoresis Analysis of a Model Protein Mixture |
| Sensitivity of Detection of the CyDye Label |
| Fluorescence Detection of S-Nitrosated Proteins in Plasma / 7： |
| Two-Dimensional Difference Gel Electrophoresis Detection of S-Nitrosated Proteins / 8： |
| Modifications of the Original Biotin-Switch Method / 9： |
| Current Procedure for the Biotin-Switch Assay / 10： |
| Conclusion / 11： |
| The Arachidonate-Dependent Survival Signaling Preventing Toxicity in Monocytes/Macrophages Exposed to Peroxynitrite / Orazio Cantoni ; Ilaria Tommasini ; Liana Cerioni |
| Materials and Methods |
| Results and Discussion |
| Practical Approaches to Investigate Redox Regulation of Heat Shock Protein Expression and Intracellular Glutathione Redox State / Vittorio Calabrese ; Anna Signorile ; Carolin Cornelius ; Cesare Mancuso ; Giovanni Scapagnini ; Bernardo Ventimiglia ; Nicolo' Ragusa ; Albena Dinkova-Kostova |
| Nitric Oxide and Cellular Stress Response: Role of Vitagenes |
| Material and Methods |
| Results |
| Discussion |
| Monitoring Oxidative Stress in Vascular Endothelial Cells in Response to Fluid Shear Stress: From Biochemical Analyses to Micro- and Nanotechnologies / Mahsa Rouhanizadeh ; Wakako Takabe ; Lisong Ai ; Hongyu Yu ; Tzung Hsiai |
| In Vitro Monitoring of Reactive Species |
| Measurement of Endothelial ROS Generation in Response to Shear Stress |
| Protein Oxidation and Nitration in Response to Shear Stress |
| Emerging Technology: Micro- and Nanotechnology |
| Summary |
| Determination of S-Nitrosothiols in Biological and Clinical Samples Using Electron Paramagnetic Resonance Spectrometry with Spin Trapping / Paul G. Winyard ; Iona A. Knight ; Frances L. Shaw ; Sophie A. Rocks ; Claire A. Davies ; Paul Eggleton ; Richard Haigh ; Matthew Whiteman ; Nigel Benjamin |
| Relative Yield of Spin-Trapped NO[superscript middle dot] from S-Nitrosoalbumin in Comparison with S-Nitrosoglutathione |
| S-Nitrosoalbumin Recovery |
| Potential Signal Contribution from N-Nitrosamines and Other Possible Sources of NO[superscript middle dot] |
| Use of the EPR Assay for S-Nitrosothiols in Combination with Isotopic Labeling |
| Novel Method for Measuring S-Nitrosothiols Using Hydrogen Sulfide / Xinjun Teng ; T. Scott Isbell ; Jack H. Crawford ; Charles A. Bosworth ; Gregory I. Giles ; Jeffrey R. Koenitzer ; Jack R. Lancaster ; Jeannette E. Doeller ; David W. Kraus ; Rakesh P. Patel |
| Experimental Manipulation of H[subscript 2]S |
| Specificity for H[subscript 2]S-Dependent Detection of RSNO |
| Comparison of H[subscript 2]S vs Tri-iodide and Copper/Cysteine Based Methods for RSNO Detection |
| Effects of pH on H[subscript 2]S-Dependent RSNO Detection |
| Effect of Antifoam |
| Summary and Conclusions |
| Kinetic Studies on Peroxynitrite Reduction by Peroxiredoxins / Madia Trujillo ; Gerardo Ferrer-Sueta ; Rafael Radi |
| General Considerations for Reagents Used |
| Kinetics Studies of the Oxidative Part of the Catalytic Cycle |
| Reductive Part of the Catalytic Cycle |
| Nitrocytochrome c: Synthesis, Purification, and Functional Studies / Laura Castro ; Adriana Maria Cassina ; Carlos Batthyany |
| Synthesis of Tyrosine-Nitrated Cytochrome c |
| Purification of the Mononitrated Cytochrome c Species |
| Mapping the Sites of Tyrosine Nitration and Spectral Characterization of the Nitrated Cytochrome c |
| Functional Consequences of Nitrated Cytochrome c |
| Concluding Remarks |
| Tyrosine Nitration, Dimerization, and Hydroxylation by Peroxynitrite in Membranes as Studied by the Hydrophobic Probe N-t-BOC-l-tyrosine tert-Butyl Ester / Silvina Bartesaghi ; Gonzalo Peluffo ; Hao Zhang ; Joy Joseph ; Balaraman Kalyanaraman12： |
| Methods |
| Assessment of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation Products / Francisco R. M. Laurindo ; Denise C. Fernandes ; Celio X. C. Santos13： |
| Probes for Assessment of Superoxide |
| Dihydroethidium as a Superoxide Probe |
| HPLC Analysis of Dihydroethidium Oxidation in Cells and Tissues: General Considerations |
| Extraction of Fluorescent DHE-Derived Products in Biological Samples |
| Quantification of Fluorescent DHE-Derived Products |
| Assessment of NADPH Oxidase Activity in Cell Membrane Fraction by DHE Oxidation |
| Methods to Measure the Reactivity of Peroxynitrite-Derived Oxidants Toward Reduced Fluoresceins and Rhodamines / Peter Wardman14： |
| Reactants |
| Sources of Error and Scope for Improvements in Methodology |
| Detection and Characterization of Peroxynitrite-Induced Modifications of Tyrosine, Tryptophan, and Methionine Residues by Tandem Mass Spectrometry / Igor Rebrin ; Catherine Bregere ; Timothy K. Gallaher ; Rajindar S. Sohal15： |
| Experimental Procedures |
| Anticipated Results |
| Reductive Gas-Phase Chemiluminescence and Flow Injection Analysis for Measurement of the Nitric Oxide Pool in Biological Matrices / Ulrike Hendgen-Cotta ; Marijke Grau ; Tienush Rassaf ; Putrika Gharini ; Malte Kelm ; Petra Kleinbongard16： |
| Circulating and Resident Pool of Nitric Oxide |
| Applied Methods for Determination of Nitrite, Nitrate, and Nitroso Species in Biological Matrices and Iron-Nitrosyls in Tissue |
| Sample Processing |
| Detection and Measurement for the Modification and Inactivation of Caspase by Nitrosative Stress In Vitro and In Vivo / Hee-Jun Na ; Hun-Taeg Chung ; Kwon-Soo Ha ; Hansoo Lee ; Young-Guen Kwon ; Timothy R. Billiar ; Young-Myeong Kim17： |
| Nitrosative Stress |
| Biological Reactivity of Nitric Oxide |
| S-Nitrosylating Chemistry |
| S-Nitrosylating Specificity |
| S-Nitrosylation of Caspases |
| Interactive Relations between Nitric Oxide (NO) and Carbon Monoxide (CO): Heme Oxygenase-1/CO Pathway Is a Key Modulator in NO-Mediated Antiapoptosis and Anti-inflammation / Byung-Min Choi18： |
| Induction of HO-1 by the NOS/NO Pathway |
| Antiapoptotic Roles of the NOS/HO-1 Pathway |
| Anti-inflammatory Roles of the NOS/HO-1 Pathway |
| Interactive Relations between NO and CO |
| Detection and Characterization of In Vivo Nitration and Oxidation of Tryptophan Residues in Proteins / 19： |
| In Vivo Real-Time Measurement of Nitric Oxide in Anesthetized Rat Brain / Rui M. Barbosa ; Catia F. Lourenco ; Ricardo M. Santos ; Francois Pomerleau ; Peter Huettl ; Greg A. Gerhardt ; Joao Laranjinha20： |
| Experimental Considerations |
| In Vivo Experiments: Results and Discussion |
| Nitric Oxide and Cardiobiology-Methods for Intact Hearts and Isolated Myocytes / Joshua M. Hare ; Farideh Beigi ; Konstantinos Tziomalos21： |
| Nitroso-Redox Signal Mediators |
| Nitric Oxide and Signaling Through Cysteine Thiols |
| Nitroso-Redox Balance or Imbalance |
| Nitric Oxide Synthases, NADPH Oxidase, and Xanthine Oxidoreductase (XOR) |
| Nitric Oxide-Redox Balance in Excitation-Contraction Coupling |
| Techniques for Assessing Oxidative and Nitrosative Stress |
| Techniques Used for Measuring S-Nitrosylation |
| Activity Measurements |
| Microscopic Technique for the Detection of Nitric Oxide-Dependent Angiogenesis in an Animal Model / Seung Namkoong ; Byoung-Hee Chung22： |
| Summary and Conclusion |
| Author Index |
| Subject Index |
| Contributors |
| Volumes in Series |
| Protein 3-Nitrotyrosine in Complex Biological Samples: Quantification by High-Pressure Liquid Chromatography/Electrochemical Detection and Emergence of Proteomic Approaches for Unbiased Identification of Modification Sites / Tal Nuriel ; Ruba S. Deeb ; David P. Hajjar ; Steven S. Gross1： |
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