| Contributors |
| Preface |
| Volumes in Series |
| Engineering of Fluorescent Reporters into Redox Domains to Monitor Electron Transfers / Derek Parsonage ; Stacy A. Reeves ; P. Andrew Karplus ; Leslie B. Poole1： |
| Introduction |
| The Problem: Low Sensitivity and Improperly Rate-Limited Assays for Redox Functions of Bacterial Peroxiredoxin Systems / 2： |
| The Solution: Engineering of Fluorescent Redox Reporters into the N-Terminal Domain of AhpF and E. coli Grx1 / 3： |
| Engineering of Disulfide-Containing Electron Acceptor Domains to Detect Electron Transfers via Fluorescence Changes; Linkage of Fluorescein to Bacterial AhpC via a Reducible Disulfide Bond / 4： |
| Materials / 5： |
| Methods / 6： |
| Summary / 7： |
| References |
| Blot-Based Detection of Dehydroalanine-Containing Glutathione Peroxidase with the Use of Biotin-Conjugated Cysteamine / Sue Goo Rhee ; Chun-Seok Cho |
| Oxidative Inactivation of Glutathione Peroxidase and the Conversion of Its Active Site Sec to DHA |
| Preparation of Biotin-Conjugated Cysteamine |
| Blot-Based Detection of DHA-GPx1 in RBCs |
| Effects of Oxidative Stress on the Formation of DHA-GPx1 in RBCs |
| Concluding Remarks |
| Acknowledgment |
| Analysis of the Redox Regulation of Protein Tyrosine Phosphatase Superfamily Members Utilizing a Cysteinyl-Labeling Assay / Benoit Boivin ; Nicholas K. Tonks |
| Active-Site Structure, Catalysis, and Oxidation |
| Detection Methods |
| General Principle of the Assay |
| Solutions |
| Preparation of the Lysis Buffer |
| Preparation of the Hypoxic Glove Box |
| Preparation of Cell Lysates / 8： |
| Cysteinyl-Labeling Assay / 9： |
| Acute Stimulus-Induced Reversible Oxidation of PTPs / 10： |
| Perspectives / 11： |
| Conclusion / 12： |
| Measuring the Redox State of Cellular Peroxiredoxins by Immunoblotting / Andrew G. Cox ; Christine C. Winterbourn ; Mark B. Hampton |
| Measurement of Prx Dimerization |
| Measurement of Prx Hyperoxidation |
| Discussion |
| Acknowledgments |
| Thiol Redox Transitions by Thioredoxin and Thioredoxin-Binding Protein-2 in Cell Signaling / Eiji Yoshihara ; Zhe Chen ; Yoshiyuki Matsuo ; Hiroshi Masutani ; Junji Yodoi |
| Functional Regulation of Redox-Sensitive Proteins by Thiol Modification |
| Thiol Reduction by the Thioredoxin Redox System |
| Thioredoxin Superfamily |
| Reversible Redox and Signal Regulation by Thioredoxin and Thioredoxin-binding Protein-2 (TBP-2) |
| Detection of Protein Thiols in Mitochondrial Oxidative Phosphorylation Complexes and Associated Proteins / Kelly K. Andringa ; Shannon M. Bailey |
| Mitochondria Isolation and Protein Thiol Labeling |
| Application of Blue Native-PAGE for the Isolation of Oxidative Phosphorylation Protein Subunits and Other Proteins Associated with the Complexes |
| Detection of IBTP-Labeled Protein Thiols in Protein Complexes |
| Analysis and Mass Spectrometry Identification of Protein |
| Other Considerations |
| Mitochondrial Thioredoxin Reductase: Purification, Inhibitor Studies, and Role in Cell Signaling / Maria Pia Rigobello ; Alberto Bindoli |
| Purification of Thioredoxin Reductase from Isolated Mitochondria, Cultured Cells, and Whole Organs |
| Estimation of Thioredoxin Reductase Activity |
| Inhibitor Studies of Thioredoxin Reductase |
| Role in Cell Signaling |
| Measuring Mitochondrial Protein Thiol Redox State / Raquel Requejo ; Edward T. Chouchani ; Thomas R. Hurd ; Katja E. Menger ; Michael P. Murphy |
| Quantification of Mitochondrial Protein Thiols |
| Quantification of Glutathionylation of Mitochondrial Proteins |
| Assessment of S-Nitrosated Protein Thiols |
| Measurement of the Thioredoxin and Peroxiredoxin Redox States |
| Conclusions |
| Measurement of Extracellular (Exofacial) Versus Intracellular Protein Thiols / Jolanta Skalska ; Steven Bernstein ; Paul Brookes |
| Measurement of Mitochondrial Thiol Status |
| Measurement of Cytosolic Thiol Status |
| Measurement of Exofacial Thiols |
| Exofacial Thiol Status and Cancer |
| Redox Clamp Model for Study of Extracellular Thiols and Disulfides in Redox Signaling / Young-Mi Go ; Dean P. Jones |
| Key Concepts for Use |
| Principles for Experimental Design |
| Summary of Available Redox Clamp Studies |
| Perspectives and Conclusion |
| Redox State of Human Serum Albumin in Terms of Cysteine-34 in Health and Disease / Karl Oettl ; Gunther Marsche |
| Background |
| HPLC Analysis |
| Albumin Thiol State and Exercise |
| Influence of Supplementation |
| Albumin Oxidation in Disease |
| Albumin Thiol State During Aging |
| Methods for Studying Redox Cycling of Thioredoxin in Mediating Preconditioning-Induced Survival Genes and Proteins / Chuang C. Chiueh |
| Hormetic Mechanism: Role of Redox Cycling of Thioredoxin |
| Redox Functioning of Thioredoxin |
| Implications of Preconditioning Protection from Preclinical and Clinical Studies |
| Drugs Mimic Thioredoxin-Medicated Preconditioning-Induced Signaling and Protection in Cells |
| Methods and Materials |
| Oxidative Stress, Thiol Redox Signaling Methods in Epigenetics / Isaac K. Sundar ; Samuel Caito ; Hongwei Yao ; Irfan Rahman13： |
| Histone Acetylation by Immunoblotting |
| HAT Activity Assay |
| HDAC Activity Assay |
| HDACs Levels by Immunoblotting |
| Posttranslational Modifications of HDACs and SIRTs (Sirtuins 1-7) by Immunoprecipitation |
| Preparation of Whole Cell Lysate |
| Preparation of Cytoplasmic and Nuclear Proteins |
| Redox-Mediated Posttranslational Modification Assays |
| Chromatin Immunoprecipitation (ChIP) Assay |
| Characterization of Protein Targets of Mammalian Thioredoxin Reductases / Anton A. Turanov ; Dolph L. Hatfield ; Vadim N. Gladyshev14： |
| Preparation of TR-immobilized Affinity Resins |
| Identification of Targets of Mammalian TRs in Cell Lysates |
| Concluding Remarks and Future Perspectives |
| Alteration of Thioredoxin Reductase 1 Levels in Elucidating Cancer Etiology / Min-Hyuk Yoo ; Bradley A. Carlson ; Petra Tsuji ; Robert Irons15： |
| Materials and Methods |
| Results and Discussion |
| Conclusions and Future Perspectives |
| Regulation of Apoptosis Signal-Regulating Kinase 1 in Redox Signaling / Kazumi Katagiri ; Atsushi Matsuzawa ; Hidenori Ichijo16： |
| Overview |
| Comment |
| Protocols for the Detection of S-Glutathionylated and S-Nitrosylated Proteins In Situ / Scott W. Aesif ; Yvonne M. W. Janssen-Heininger ; Niki L. Reynaert17： |
| Protein S-Glutathionylation |
| Protein S-Nitrosylation |
| Synthesis, Quantification, Characterization, and Signaling Properties of Glutathionyl Conjugates of Enals / Sanjay Srivastava ; Kota V. Ramana ; Aruni Bhatnagar ; Satish K. Srivastava18： |
| Synthesis, Quantification, and Characterization of Reagent Glutathionyl Conjugates of HNE |
| Metabolism of HNE |
| Signaling Properties of Glutathionyl Conjugates of HNE |
| Thioredoxin and Redox Signaling in Vasculature-Studies Using Trx2 Endothelium-Specific Transgenic Mice / Wang Min ; Luyao (Kevin) Xu ; Huanjiao (Jenny) Zhou ; Qunhua Huang ; Haifeng Zhang ; Yun He ; Xu Zhe ; Yan Luo19： |
| Author Index |
| Subject Index |
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図書
