| Preface |
| Acknowledgements |
| General Aspects |
| Rate acceleration in enzyme-catalyzed reactions |
| Michaelis-Menten kinetics |
| Enzyme inhibition |
| Specificity |
| Improvement or alteration of enzyme specifity |
| Enzyme stabilization and reactor configuration |
| Cofactor regeneration |
| Enzyme catalysis in organic solvents |
| Multienzyme systems and metabolic engineering |
| Rational design of new enzymatic catalysts |
| Conclusion |
| References |
| Use of Hydrolytic Enzymes: Amidases, Proteases, Esterases, Lipases, Nitrilases, Phosphatases, Epoxide Hydrolases |
| Amidases |
| Protease-catalyzed peptide synthesis |
| Proteases that act as esterases |
| Acetylcholine esterase |
| Pig liver esterase |
| Phospholipases |
| Cholesterol esterase |
| Lipases |
| Nitrile hydrolysis enzymes |
| Epoxide hydrolase |
| Phosphatase |
| Oxidoreductions |
| Nicotinamide cofactor dependent oxidoreductions |
| Dehydrogenases which utilize ketoacids as substrates |
| Other NAD(P)-dependent dehydrogenases |
| Oxidoreductases that are metalloenzymes |
| C-C Bond Formation |
| Aldol condensation |
| Ketol and aldol transfer reaction |
| Addition of HCN to aldehydes |
| Acyloin condensation |
| C-C bond forming reactions involving acetyl co |
| Isoprenoid and steroid synthesis. bgr;-replacement of chloroalanine / A： |
| C-C bond formation catalyzed by vitamin B 12 |
| Synthesis of Glycoside Bonds |
| Background |
| Glycosyltransferases of the Leloir pathway |
| Substrate specificity and synthetic applications of glycosyltransferases |
| Non-Leloir glycosyltransferases |
| Glycosidases |
| Transglycosidases |
| Synthesis of N-glycosides |
| Biological applications of synthetic glycoconjugates |
| Future opportunities |
| Addition, Elimination and Other Group Transfer Reactions (Phosphoryl-, Methyl-, Sulpho- and Amino-Transfer Reactions) |
| Addition of water to alkenes: fumarase |
| Addition of ammonia to double bonds: ammonia lyases |
| Transamination: aminotransferases |
| Addition and elimination of carboxyl group |
| Nucleoside triphosphate requiring enzymatic reactions |
| Preparation of ATP chiral at agr;-, bgr;- or ggr;-phosphorous |
| Phosphorothioate-containing DNA and RNA. DNA and RNA oligomers |
| Incorporation of modified or unnatural bases into DNA or RNA |
| Dehalogenation |
| Synthesis of chiral methyl groups |
| S-Adenosylmethionine and transmethylation |
| Sulfate activation and transfer reactions |
| Subject index |
