Preface |
Contributors |
Biochemical Methods to Study the Ubiquitin-Proteasome System / Part I: |
Small-Molecule Inhibitors of Proteasome Activity / Maria Gaczynska ; Pawel A. Osmulski1: |
Purification of E1 and E1-Like Enzymes / Arthur L. Haas2: |
Assays for RING Family Ubiquitin Ligases / Manabu Furukawa ; Paul S. Andrews ; Yue Xiong3: |
Ubiquitin Chain Synthesis / Shahri Raasi ; Cecile M. Pickart4: |
Purification of Proteasomes, Proteasome Subcomplexes, and Proteasome-Associated Proteins From Budding Yeast / David S. Leggett ; Michael H. Glickman ; Daniel Finley5: |
Recognition and Processing of Misfolded Proteins by PA700, the 19S Regulatory Complex of the 26S Proteasome / Chang-Wei Liu ; Elizabeth Strickland ; George N. DeMartino ; Philip J. Thomas6: |
Cell-Free Assay for Ubiquitin-Independent Proteasomal Protein Degradation / Chaim Kahana ; Yuval Reiss7: |
Assays of Proteasome-Dependent Cleavage Products / Stefan Tenzer ; Hansjörg Schild8: |
Identification of Components of Protein Complexes / Carol E. Parker ; Maria R. Warren ; David R. Loiselle ; Nedyalka N. Dicheva ; Cameron O. Scarlett ; Christoph H. Borchers9: |
Mass Spectrometric Determination of Protein Ubiquitination / Viorel Mocanu ; Susanna F. Greer10: |
Reconstitution of Endoplasmic Reticulum-Associated Degradation Using Yeast Membranes and Cytosol / Robert J. Lee ; Ardythe A. McCracken ; Jeffrey L. Brodsky11: |
Reticulocyte Lysate as a Model System to Study Endoplasmic Reticulum Membrane Protein Degradation / Eric Carlson ; Nathan Bays ; Larry David ; William R. Skach12: |
Deubiquitinating Enzyme Purification, Assay Inhibitors, and Characterization / Nathaniel S. Russell ; Keith D. Wilkinson13: |
Cellular Methods to Study Ubiquitin-Proteasome-Dependent Functions / Part II: |
Measuring Ubiquitin Conjugation in Cells / Edward G. Mimnaugh ; Leonard M. Neckers14: |
Assays for Proteasome Assembly and Maturation / R. Jürgen Dohmen ; Markus K. London ; Christoph Glanemann ; Paula C. Ramos15: |
N-Terminal Ubiquitination / Aaron Ciechanover16: |
Quantitating Defective Ribosome Products / Shu-Bing Qian ; Jack R. Bennink ; Jonathan W. Yewdell17: |
Endoplasmic Reticulum-Associated Protein Quality Control and Degradation: Screen for ERAD Mutants After Ethylmethane Sulfonate Mutagenesis / Antje Schäfer ; Dieter H. Wolf18: |
Endoplasmic Reticulum-Associated Protein Quality Control and Degradation: Genome Wide Screen for ERAD Components / 19: |
Cystic Fibrosis Transmembrane Conductance Regulator as a Model Substrate to Study Endoplasmic Reticulum Protein Quality Control in Mammalian Cells / J. Michael Younger ; Chun-Yang Fan ; Liling Chen ; Meredith F. N. Rosser ; Cam Patterson ; Douglas M. Cyr20: |
Aggresome Formation / Michael J. Corboy ; W. Christian Wigley21: |
Detection of Sumoylated Proteins / Roland S. Hilgarth ; Kevin D. Sarge22: |
Proteasome Inhibitors in Cancer Therapy / Robert Z. Orlowski23: |
Parkinson's Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin / Michael G. Schlossmacher ; Hideki Shimura24: |
Index |
Preface |
Contributors |
Biochemical Methods to Study the Ubiquitin-Proteasome System / Part I: |