Primary and Tertiary Structure / M. Cygler ; J.D. Schrag
Structure as a Basis for Understanding the Interfacial Properties of Lipases / F. Drablos ; S.B. Petersen
Identification of Conserved Residues in a Family of Esterase and Lipase Sequences / M.T. Neves ; E.I. Petersen
Identification of Important Motifs in Protein Sequences: The Program Multim and Its Applications to Lipase Related Sequences
Lipases and the (/( Hydrolase Fold / C. Cambillau ; Y. Bourne ; M.P. Egloff ; C. Martinez ; H. van Tilbeurgh
Pancreatic Lipases and Their Complexes with Colipases and Inhibitors: Crystallization and Crystal Packing / M.R. Egmond ; C.J.van Bemmel
Impact of Structural Information on Understanding Lipolytic Function / P. Martel
Surface and Electrostatics of Cutinases
Isolation, Cloning, Expression, and Engineering / M.E. Lowe
Site-Specific Mutagenesis of Human Pancreatic Lipase / H. Wong ; R.C. Davis ; J.S. Hill ; D. Yang ; M. Schotz
Lipase Engineering: A Window into Structure-Function Relationships / L. Blackberg ; R.-D. Duan ; B. Sternby
Purification of Carboxyl Ester Lipase (Bile Salt-Stimulated Lipase) from Human Milk and Pancreas / C. Schmidt-Dannert ; M.L. R a ; R.D. Schmid
Two Novel Lipases from the Thermophile Bacillus thermocatenulatus: Screening, Purification, Cloning, Overexpression, and Properties / P. Adlercreutz ; T. Gitlesen ; I. Ncube ; J.S. Read
Vernonia Lipase: A Plant Lipase with Strong Fatty Acid Selectivity / M.C. Schotz
Hepatic Lipase: High Level Expression and Subunit Structure Determination / L. Alberghina ; M. Lotti
Cloning, Sequencing, and Expression of Candida rugosa Lipases / L. Dupuis ; S. Canaan ; M. Riviore ; C. Wicker-Planquart
Influence of Various Signal Peptides onSecretion of Mammalian Acidic Lipases in the Baculovirus/Insect Cell System / C. Holm ; J.A. Contreras ; R. Verger
Large-Scale Purification and Kinetic Properties of Recombinant Hormone-Sensitive Lipase from Baculovirus/Insect Cell Systems
New Pancreatic Lipases: Gene Expression, Protein Secretion, and the Newborn / M. Boston ; C. Requadt ; S. Danko ; A. Jarnagin ; E. Ashizawa ; S. Wu ; A.J. Poulose ; R. Bott
Structure and Function of Engineered Pseudomonas mendocina Lipase / A. Svendsen ; I.G. Clausen ; S.A. Patkar ; K. Borch ; M. Thellersen
Protein Engineering of Microbial Lipases with Industrial Interest / E. Mas ; M.-O. Sadoulet ; A. El Battari ; D. Lombardo
Glycosylation of Bile Salt-Dependent Lipase (Cholesterol Esterase) / L. Haalck ; F. Paltauf ; J. Pleiss ; F. Spener ; P. Stadler
Steroselectivity of Lipase from Rhizopus oryzae toward Triacylglycerols and Analogs: Computer Aided Modeling and Experimental Validation
Author Index
Subject Index
Primary and Tertiary Structure / M. Cygler ; J.D. Schrag
Structure as a Basis for Understanding the Interfacial Properties of Lipases / F. Drablos ; S.B. Petersen
Identification of Conserved Residues in a Family of Esterase and Lipase Sequences / M.T. Neves ; E.I. Petersen
Regulation and Characterization / A.B.R. Thomson ; A. De Pover ; M. Keelan ; E. Jarocka-Cyrta ; M.T. Clandinin
Inhibition of Lipid Absorption as an Approach to the Treatment of Obesity: An Overview / C. Holm ; D. Langin ; V. Manganiello ; P. Belfrage ; E. Degerman
Regulation of Hormone-Sensitive Lipase Activity in Adipose Tissue / D.Y. Hui
Use of Gene Knockout Mice to Establish Lipase Function / R. Zolfaghari ; R. Shamir ; E.A. Fisher
Properties of Pancreatic Carboxyl Ester Lipase in mRNA-Injected Xenopus Oocytes and Transfected Mammalian Hepatic and Intestinal Cells / G. Olivecrona ; A. Lookene
Noncatalytic Functions of Lipoprotein Lipase / E.H. Harrison ; E.S. Kempner
Radiation Inactivation Studies of Hepatic Cholesteryl Ester Hydrolases / M. Aoubala ; I. Douchet ; S. Bezzine ; M. Hirn ; R. Verger ; A. De Caro
Immunological Techniques for the Characterization of Digestive Lipases
Substrate and Inhibitor Characterization / D.M. Small
Physical Behavior of Lipase Substrates / T. Hongel ; K. Jorgensen ; D. Stokes ; R.L. Biltonen ; O.G. Mouritsen
Phospholipase A2 Activity and Physical Properties of Lipid-Bilayer Substrates / S. Ransac ; Y. Gargouri ; F. Marguet ; G. Buono ; C. Beglinger ; P. Hildebrand ; H. Lengsfeld ; P. Hadvary
Covalent Inactivation of Lipases / S.R. Feaster ; D.M Quinn
Mechanism-Based Inhibitors of Mammalian Cholesterol Esterase / L. Haalck ; F. Spener
On the Inhibition of Microbial Lipases by Tetrahydrolipstatin / M. Ivanova ; I. Panaiotov
Recovery of Monomolecular Files in Studies of Lipolysis / S. Labourdenne ; A. Cagna ; B. Delorme ; G. Esposito ; C. Riviere
Oil-Drop Tensiometer: Applications for Studying the Kinetics of Lipase Action / F. Ferrato ; F. Carriere ; L. Sarda
A Critical Reevaluation of the Phenomenon of "Interfacial Activation"
Biocatalytic Utility / U. Ader ; P. Andersch ; M. Berger ; U. Goergens ; B. Haase ; J. Hermann ; K. Laumen ; R. Seemayer ; C. Waldinger ; M.P. Schneider
Screening Techniques for Lipase Catalyst Selection / K. Hult ; M. Holmquist
Kinetics, Molecular Modeling, and Synthetic Applications with Microbial Lipases / M. Lobell
Ester Synthesis via AcylTransfer Transesterification / J.J. Lalonde ; M.A. Navia ; A.L. Margolin
Cross-Linked Enzyme Crystals of Lipases as Catalysts for Kinetic Resolution of Acids and Alcohols / G.A. Hutcheon ; P.J. Halling ; B.D. Moore
Measurement and Control of Hydration in Nonaqueous Biocatalysis / T. Anthonsen ; J.A. Jongejan
Solvent Effect in Lipase-Catalyzed Racemate Resolution / E. Cernia ; C. Palocci
Lipases in Supercritical Fluids / H. K.Weber ; K. Faber
Stabilization of Lipases against Deactivation of Acetaldehyde Formed in Acyl Transfer Reactions
Author Index
Subject Index
Regulation and Characterization / A.B.R. Thomson ; A. De Pover ; M. Keelan ; E. Jarocka-Cyrta ; M.T. Clandinin
Inhibition of Lipid Absorption as an Approach to the Treatment of Obesity: An Overview / C. Holm ; D. Langin ; V. Manganiello ; P. Belfrage ; E. Degerman
Regulation of Hormone-Sensitive Lipase Activity in Adipose Tissue / D.Y. Hui
C-X-C Chemokines / R. Horuk ; D. Reilly ; D. Yansura
Expression, Purification, and Characterization of Escherichia coli Derived Recombinant Human Melanoma Growth Stimulating Activity / A. Wuyts ; P. Proost ; G. Froyen ; A. Haelens ; A. Billiau ; G. Opdenakker ; J. Van Damme
Purification and Identification of Human and Mouse Granulocyte Chemotactic Protein-2 Isoforms / P.E. Dawson
Synthesis of Chemokines by Native Chemical Ligation / W.J. Fairbrother ; H.B. Lowman
Molecular Approaches to Structure-Function Analysis of Interleukin-8 / J. Hesselgesser
Alanine Scan Mutagenesis of Chemokines / D. Baly ; U. Gibson ; D. Allison ; L. DeForge
Biological Assays for C-X-C Chemokines / K. Rajarathnam ; C.M. Kay ; I. Clark-Lewis ; B.D. Sykes
Characterization of Quaternary Structure of Interleukin-8 and Functional Implications
C-C and C Chemokines / S. Struyf ; P. Allavena ; S. Sozzani ; A. Mantovani
Isolation of Human Monocyte Chemotactic Proteins and Study of their Producer and Responder Cells by Immunotests and Bioassays / A.-M. Buckle ; S. Craig ; L.G. Czaplewski
Assays for Macrophage Inflammatory Proteins / P.J. Nelson ; J.M. Pattison ; A.M. Krensky
Gene Expression of Rantes
Expression of Chemokine Rantes and Production of Monoclonal Antibodies / R.C. Newton ; K. Vaddi
Biological Responses to C-C Chemokines / D.N. Cook
Gene Targeting Strategies to Study Chemokine Function in Vivo / J.A. Hedrick ; A. Zlotnik
Lymphotactin: A New Class of Chemokine / J.M. Schrider
Isolation and Purification of Neutrophil Activating Peptide 4: A Chemokine Missing Two Cysteines
Other Methods / L. Vo ; P. Owen ; J. Anderson
Chemical Synthesis Purification and Folding of C-X-C and C-C Chemokines / J.B. Smith ; H.R. Herschmann
Identification of Inflammatory Mediators by Screening for Glucocorticoid-Attenuated Response Genes / D.D. Taub ; M.L. Key ; D.L. Longo ; W.J. Murphy
Chemokine-Induced Human Lymphocyte Infiltration and Engraftment in huPBL-SCID Mice / D.A. Barnes ; H.D. Perez
Transgenic Methods to Study Chemokine Function in Lung and Central Nervous System / R.M. Ransohoff ; M Tani ; A.R. Glabinski ; A. Chernosky ; K. Krivacic ; J.W. Peterson ; H.-F. Chien ; B.D. Trapp
Chemokines and Chemokine Receptors in Model Neurological Pathologies: Molecular and Immunocytochemical Approaches / R.E. Offord ; H.F. Gaertner ; T.N.C. Wells ; A.E.I. Proudfoot
Synthesis and Evaluation of Fluorescent Chemokines Labeled at the Amino Terminal / G.S.V. Kuschert ; R.E. Hubbard ; C.A. Power ; A.J. Hoogewerf
Solid-Phase Binding Assay to Study Interaction of Chemokines with Glycosaminoglycans / M. Conklyn ; H.J. Showell
Biological Activity of C-X-C and C-C Chemokines in Leukocyte Subpopulations in Human Whole Blood
Author Index
Subject Index
C-X-C Chemokines / R. Horuk ; D. Reilly ; D. Yansura
Expression, Purification, and Characterization of Escherichia coli Derived Recombinant Human Melanoma Growth Stimulating Activity / A. Wuyts ; P. Proost ; G. Froyen ; A. Haelens ; A. Billiau ; G. Opdenakker ; J. Van Damme
Purification and Identification of Human and Mouse Granulocyte Chemotactic Protein-2 Isoforms / P.E. Dawson
Purification and Reconstitution of Cyclin-Dependent Kinase 2 in Four States of Activity / H. Wilhelm ; S.S.L. Andersen ; E. Karsenti
Purification of Recombinant Cyclin B1/Cdc2 Kinase from Xenopus Egg Extracts / K.A. Resing ; N.G. Ahn
Protein Phosphorylation Analysis by Electrospray Ionization Mass Spectrometry / C. Norbury ; S. Moreno
Cloning Cell Cycle Regulatory Genes by Transcomplementation in Yeast / P.D. Adams ; P. Lopez ; W.R. Sellers ; W.G. Kaelin, Jr.
Fluorescence-Activated Cell Sorting of Transfected Cells / N.J.C. Lamb ; A. Fernandez
Microinjection of Antibodies into Mammalian Cells / K.D. Lustig ; P.T. Stukenberg ; T.J. McGarry ; R.W. King ; V.L. Cryns ; P.E. Mead ; L.I. Zon ; J. Yuan ; M.W. Kirschner
Small Pool Expression Screening: Identification of Genes Involved in Cell Cycle Control, Apoptosis, and Early Development / J. Pines
Localization of Cell Cycle Regulators by Immunofluorescence / L. Meijer ; S.-H. Kim
Chemical Inhibitors of Cyclin-Dependent Kinases / D.E. Bassett, Jr. ; M. Boguski ; P. Hieter
Identifying Human Homologs of Cell Cycle Genes using dbEST and XREFdb / C. Bai ; S.J. Elledge
Gene Identification Using the Yeast Two-Hybrid System
Cell Cycle Regulators in Mammalian Systems / S. Freundlieb ; U. Baron ; A.L. Bonin ; M. Gossen ; H. Bujard
Use of Tetracycline-Controlled Gene Expression Systems to Study Mammalian Cell Cycle / R.J. Sheaff
Regulation of Mammalian Cyclin-Dependent Kinase 2 / D.E. Phelps ; Y. Xiong
Assay for Activity of Mammalian Cyclin D-Dependent Kinases CDK4 and CDK6 / J.R. Nevins ; J. DeGregori ; L. Jakoi ; G. Leone
Functional Analysis of E2F Transcription Factor / L. Stepanova ; X. Leng ; J.W. Harper
Analysis of Mammalian Cdc37, A Protein Kinase Targeting Subunit of Heat Shock Protein 90 / B.D. Dynlacht ; C. Ngwu ; J. Winston ; E.C. Swindell ; E. Harlow
Purification and Analysis of CIP/KIP Proteins / L. Jayaraman ; E. Freulick ; C. Prives
Functional Dissection of the p53 Tumor Suppressor Protein / R.P. Fisher
Role of Transforming Growth Factor ( and Decorin in Controlling Fibrosis
Extracellular Matrix in Invertebrates / J.E. Schwarzbauer ; F. Musset-Bilal ; C.S. Ryan
Extracellular Calcium-Binding Protein SPARC/Osteonectin in Caenorhabditis elegans / L.I. Fessler ; R.E. Nelson ; J.H. Fessler
Drosophila Extracellular Matrix
Modern Methods in Extracellular Matrix Research / F.G. Giancotti ; L. Spinardi ; F. Mainiero ; R. Sanders
Expression of Heterologous Integrin Genes in Cultured Eukaryotic Cells / B.G. Hoffstrom ; E.A. Wayner
Immunohistochemical Techniques in the Study of Extracellular Matrix and Its Receptors / E. Koivunen ; B. Wang ; C.D. Dickinson ; E. Ruoslahti
Peptides in Cell Adhesion Research / K.T. ONeil ; W.F. DeGrado ; S.A. Mousa ; N. Ramachandran ; R.H. Hoess
Identification of Recognition Sequences of Adhesion Molecules Using Phage Display Technology / E.L. George ; R.O.Hynes
Gene Targeting and Generation of Mutant Mice for Studies of Cell(Extracellular Matrix Interactions / R. Pytela ; S. Suzuki ; J. Breuss ; D.J. Erle ; D. Sheppard
Polymerase Chain Reaction Cloning with Degenerate Primers: Homology-Based Identification of Adhesion Molecules / M.J. Williams ; I.D. Campbell
Solution Structures of Modular Proteins by Nuclear Magnetic Resonance / J. Engel
Electron Microscopy of Extracellular Matrix Components / P.D. Yurchenco ; J.J. ORear
Basement Membrane Assembly / J.A. McDonald
Extracellular Matrix Assembly
Use of Extracellular Matrix / R.J. Blaschke ; A.R. Howlett ; P.-Y. Desprez ; O.W. Petersen ; M.J. Bissell
Cell Differentiation by Extracellular Matrix Components / J.F. Tschopp ; W.S. Craig ; J. Tolley ; J. Blevitt ; C. Mazur
Bacterial Expression and Purification of Human Protein Prenyltransferases Using Epitope-Tagged, Translationally Coupled Systems / J.F. Moomaw ; F.L. Zhang ; P.J. Casey
Isolation of Protein Prenyltransferases from Bovine Brain and Baculovirus Expression System / Y. Reiss
Substrate Interactions of Protein Prenyltransferases / P.B. Cassidy ; J.M. Dolence ; C.D. Poulter
Continuous Fluorescence Assay for Protein Prenyltransferases / H. Mitsuzawa ; F. Tamanoi
In Vivo Assays for Farnesyltransferase Inhibitors with Saccharomyces cerevisiae / B.E. Caplin ; M.S. Marshall
Mutagenesis and Biochemical Analysis of Recombinant Yeast Prenyltransferases / D.A. Mitchell ; R.J. Deschenes
Characterization of Protein Prenylation in Saccharomyces cerevisiae / A.L. Wilson ; W.A. Maltese
Coupled Translation/Prenylation of Rab Proteins in Vitro / Y. Fukada
Prenylation and Carboxylmethylation of G-Protein gamma Subunit / A.D. Cox
Mutation and Analysis of Prenylation Signal Sequences / Y. Takai ; K. Kaibuchi ; A. Kikuci ; T. Sasaki
Effects of Prenyl Modifications on Interactions of Small G Proteins with Regulators / M.Dalton ; M. Sinensky
Expression Systems for Nuclear Lamin Proteins: Farnesylation in Assembly of Nuclear Lamina / J. Inglese
Prenylation-Dependent Targeting of G-Protein-Coupled Receptor Kinases / J.A. Thissen ; M.G. Barrett
Prenylated Peptides in Identification of Specific Binding Proteins / R.M. Epand ; F. Naider ; J.M. Becker
Lipid-Mediated a-Factor Interactions with Artificial Membranes
Prenylation-Dependent Processing / L. Liu ; G.-F. Jang ; C.C.Farnsworth ; K. Yokoyama ; J.A. Glomset ; M.H. Gelb
Synthetic Prenylated Peptides: Studying Prenyl Protein-Specific Endoprotease and Other Aspects of Protein Prenylation / B.A. Gilbert ; Y.-T. Ma ; R.R. Rando
Inhibitors of Prenylated ProteinEndoprotease / C. Volker ; M.H. Pillinger ; M.R. Philips ; J.B. Stock
Prenylcysteine Analogs to Study Function of Carboxylmethylation in Signal Transduction
Farnesylcysteine Analogs to Probe Role of Prenylated Protein Methyltransferase / M.N. Ashby ; J. Rine
Ras and a-Factor Converting Enzyme / C.A. Hrycyna ; S.J. Wait ; P.S. Backlund, Jr. ; S. Michaelis
Yeast STE14 Methyltransferase, Expressed as TrpE-STE14 Fusion Protein in Escherichia coli, for in Vitro Carboxylmethylation of Prenylated Polypeptides
Palmitoylation / B.M. Willumsen
Analysis of Ras Acylation Sites: Mutagenesis and Transfection / S.I. Patterson ; J.H.P. Skene
Inhibition of Dynamic Protein Palmitoylation in Intact Cells with Tunicamycin / M. Bouvier ; P. Chidiac ; T.E. Hebert ; T.P. Loisel ; S. Moffett ; B. Mouillac
Dynamic Palmitoylation of G-Protein-Coupled Receptors in Eukaryotic Cells / M.E. Linder ; C. Kleuss ; S.M. Mumby
Palmitoylation of G-Protein ( Subunits / A.I. Magee ; J. Wootton ; J. de Bony
Assay and Isolation of Palmitoyl-Protein Thioesterase from Bovine Brain Using Palmitoylated H-Ras as Substrate / D.R. Pepperberg ; D.F. Morrison ; P.J. OBrien
Prenylation / C.A. Omer ; R.E. Diehl ; A.M. Kral
Bacterial Expression and Purification of Human Protein Prenyltransferases Using Epitope-Tagged, Translationally Coupled Systems / J.F. Moomaw ; F.L. Zhang ; P.J. Casey
Isolation of Protein Prenyltransferases from Bovine Brain and Baculovirus Expression System / Y. Reiss
Thiol/Disulfide Exchange Equilibria and Disulfide Bond Stability
Thiyl Radicals / P. Wardman ; C. von Sonntag
Kinetic Factors That Control the Fate of Thiyl Radicals in Cells / C. Schineich
Kinetics of Thiol Reactions / S.A. Everett
Perthiols as Antioxidants: Radical-Scavenging and Pro-oxidative Mechanisms / S.C. Kundu ; R.L. Willson
Thiyl (Sulfhydryl/Thiol) Free Radical Reactions, Vitamins, Beta-Carotene, and Superoxide Dismutase in Oxidative Stress
Design and Interpretation of Enzymatic Studies / C.C. Winterbourn ; D. Metodiewa
Reaction of Superoxide with Glutathione and Other Thiols / L.M. Weiner
Quantitative Determination of Thiol Groups in Low and High Molecular Weight Compounds by Electron Paramagnetic Resonance / E. Cadenas
Thiyl Radical Formation during Thiol Oxidation by Ferrylmyoglobin / R. Munday
In Vivo Toxicity of Thiols: Relationship to Rate of One-Electron Oxidation by Oxyhemoglobin
Chemical Basis of Thiol/Disulfide Measurements / N.S. Kosower ; E.M. Kosower
Diamide: An Oxidant Probe for Thiols
Bromobimane Probes for Thiols / G.L. Newton ; R.C. Fahey
Determination of Biothiols by Bromobimane Labeling and High-Performance Liquid Chromatography / R. Singh ; G.V. Lamoureux ; W.J. Lees ; G.M. Whitesides
Reagents for Rapid Reduction of Disulfide Bonds / K. Becker ; R.H. Schirmer
1,3-Bis(2-chloroethyl)-1-nitrosourea as Thiol-Carbamoylating Agent in Biological Systems
Monothiols: Measurement in Organs, Cells, Organelles, and Body Fluids / H. Nohl ; K. Stolze
Noninvasive Measurement of Thiol Levels in Cells and Isolated Organs / J.A. Cook ; J.B. Mitchell
Measurement of Thiols in Cell Populations from Tumor and Normal Tissue / C. Hwang ; H.F. Lodish ; A.J. Sinskey
Measurement of Glutathione Redox State in Cytosol and Secretory Pathway of Cultured Cells / T.Y. Aw
Assay of Thiols and Disulfides in Intestinal Lymph / W.A. Blattler ; A.R. Collinson
Assay for Thiols Based on Reactivation of Papain / J. Vita ; J. Sastre ; M. Asensi ; L. Packer
Assay of Blood Glutathione Oxidation during Physical Exercise / P. Reinemer ; H.W. Dirr ; R. Huber
X-Ray Structure Methods for Glutathione Binding / W. Drige ; R. Kinscherf ; S. Mihm ; D. Galter ; S. Roth ; H. Gmander ; T. Fischbach ; M. Bockstette
Thiols and the Immune System: Effect of N-Acetylcysteine on T Cell System in Human Subjects / R. Olivier
Flow Cytometry Technique for Assessing Effects of N-Acetylcysteine on Apoptosis and Cell Viability of Human Immunodeficiency Virus-Infected Lymphocytes / C. Gitler ; M. Londner
Use of p-Nitrophenyl Disulfide to Measure Reductive Capacity of Intact Cells / R.L. Krauth-Siegel ; E.M. Jacoby
Trypanothione and N 1 -Glutathionylspermidine: Isolation and Determination / D.M. Ziegler ; L.L. Poulsen
Estimation of Tissue Cysteamine by Quantitative Thin-Layer Chromatography
Dithiols: Alpha-Lipoic Acid / G.P. Biewenga ; A. Bast
Reaction of Lipoic Acid with Ebselen and Hypochlorous Acid / D. Han ; G.J. Handelman
An
Overview / A. Meister
Glutathione Metabolism / H.F. Gilbert
Thiol/Disulfide Exchange Equilibria and Disulfide Bond Stability
Purification and Characterization of DNA Polymerases / C.M. Joyce ; V. Derbyshire
Purification of Escherichia coli DNA Polymerase I and Klenow Fragment / H. Cai ; H. Yu ; K. McEntee ; M.F. Goodman
Purification and Properties of DNA Polymerase II from Escherichia coli / M.G. Cull ; C.S. McHenry
Purification of Escherichia coli DNA Polymerase III Holoenzyme / M.H. Barnes ; N.C. Brown
Purification of DNA Polymerase III of Gram-Positive Bacteria / J.M. Lazaro ; L. Blanco ; M. Salas
Purification of Bacteriophage phi29 DNA Polymerase / P.M.J. Burgers
DNA Polymerases from Saccharomyces cerevisiae / P.G. Mitsis ; C.-S. Chiang ; I.R. Lehman
Purification of DNA Polymerase-Primase (DNA Polymerase alpha) and DNA Polymerase delta from Embryos of Drosophila melanogaster / T.S.-F. Wang ; W.C. Copeland ; L. Rogge ; Q. Dong
Purification of Mammalian DNA Polymerases: DNA Polymerase alpha / K.M. Downey ; A.G. So
Purification of Mammalian Polymerases: DNA Polymerase delta / G.S.-J. Chui ; S. Linn
Purification of Mammalian Polymerases: DNA Polymerase epsilon / W.A. Beard ; S.H. Wilson
Purification and Domain-Mapping of Mammalian DNA Polymerasebeta / M.E. Budd ; J.L. Campbell
Purification and Enzymatic and Functional Characterization of DNA Polymerase beta-like Enzyme POL4
Expressed during Yeast Meiosis / S.F.J. Le Grice ; C.E. Cameron ; S.J. Benkovic
Purification and Characterization of Human Immunodeficiency Virus Type 1 Reverse Transcriptase
Structural Analysis of DNA Polymerases / G.P. Mullen
Solution Structure of DNA Polymerases and DNA Polymerase-Substrate Complexes / A.D. Clark, Jr. ; A. Jacobo-Molina ; P. Clark ; S.H. Hughes ; E. Arnold
Crystallization of Human Immunodeficiency Virus Type 1 Reverse Transcriptase with and without Nucleic Acid Substrates, Inhibitors, and an Antibody Fab Fragment
Mechanisms of DNA Polymerases / F. Eckstein ; J.B. Thomson
Phosphate Analogs for Study of DNA Polymerases / G.E. Wright
Mechanisms of Inhibition of DNA Polymerases by 2'-Deoxyribonucleoside 5'-Triphosphate Analogs / K. Bebenek ; T.A. Kunkel
Analyzing the Fidelity of DNA Polymerases / S. Creighton ; L.B. Bloom
Gel Fidelity Assay Measuring Nucleotide Misinsertion, Exonucleolytic Proofreading, and Lesion Bypass Efficiencies
Kinetic Analysis of Nucleotide Incorporation and Misincorporation by the Klenow Fragment of Escherichia coli DNA Polymerase I / R.A. Bambara ; P.J. Fay ; L.M. Mallaber
Methods of Analyzing Processivity
Structure-Function Studies of DNA Polymerases / L.Blanco
Mutational Analysis of Bateriophage phi29 DNA Polymerase
Rationale for Mutagenesis of DNA Polymerase Active Sites: DNA Polymerase alpha / P. Digard ; W.R. Bebrin ; D.M. Coen
Mutational Analysis of DNA Polymerase Substrate Recognition and Subunit Interactions Using Herpes Simplex Virus as Prototype / L.J. Reha-Krantz
Use of Genetic Analyses to Probe Structure, Function, and Dynamics of Bacteriophage T4 DNA Polymerase / W.H. Konigsberg
Limited Proteolysis of DNA Polymerases as Probe of Functional Domains / A. Telesnitsky ; S. Blain ; S.<$$$>
Purification and Characterization of DNA Polymerases / C.M. Joyce ; V. Derbyshire
Purification of Escherichia coli DNA Polymerase I and Klenow Fragment / H. Cai ; H. Yu ; K. McEntee ; M.F. Goodman
Purification and Properties of DNA Polymerase II from Escherichia coli / M.G. Cull ; C.S. McHenry
How to Generate Accurate Solution Structures of Double-Helical Nucleic Acid Fragments Using Nuclear Magnetic Resonance and Restrained Molecular Dynamics / B.A. Luxon ; D.G. Gorenstein
Comparison of X-ray and NMR-Determined Nucleic Acid Structures / P.K. Radha ; R. Nibedita ; R.A. Kumar ; R.V. Hosur
Distance Geometry in NMR Determination of Solution Conformation of Nucleic Acids: Application to d-ACCGTTAACGGT / N.B. Ulyanov
Statistical Analysis of DNA Duplex Structural Features / M.A. Young ; J. Srinivasan ; I. Goljer ; S. Kumar ; D.L. Beveridge ; P.H. Bolton
Structure Determination and Analysis of Local Bending in an A-Tract DNA Duplex: Comparison of Results from Crystallography
Nuclear Magnetic Resonance, and Molecular Dynamics Simulation on d(CGCAAAAATGCG) / G.V. Fazakerley ; Y. Boulard
DNA Mismatches and Modified Bases / G. Carlstrim ; S.-m. Chen ; S. Miick ; W.J. Chazin
NMR Studies of Complex DNA Structures: The Holliday Junction Intermediate in Genetic Recombination / N.B. Leontis ; M.E. Piotto ; M.T. Hills ; A. Malhotra ; I.V. Ouporov ; J.M. Nussbaum
Structural Studies of DNA Three-Way Junctions / M.W. Germann ; N. Zhou ; J.H. van de Sande ; H.J. Vogel
Parallel-Stranded Duplex DNA: An NMR Perspective / J. Feigon ; K.M. Koshlap ; F.W. Smith
1 H NMR Spectroscopy of DNA Triplexes and Quadruplexes / C.-L. Juang ; P.Tang ; G.S. Harbison
Solid-State NMR of DNA / P.F. Agris ; S.C. Brown
Systems for the NMR Study of Modified Nucleoside-Dependent
Preparation of Isotopically Enriched RNAs for Heteronuclear NMR / J.D. Puglisi ; J.R. Wyatt
Biochemical and NMR Studies of RNA Conformation with an Emphasis on RNA Pseudoknots / A. Pardi
Multidimensional Heteronuclear NMR Experiments for Structure Determination of Isotopically Labeled RNA. Nucleic Acid Dynamics / M. Gueron ; J.-L. Leroy
Studies of Base-Pair Kinetics by NMR Measurement of Proton Exchange / A.N. Lane
Determination of Fast Dynamics of Nucleic Acids by NMR / G.C. King ; J.W. Harper ; Z. Xi
Isotope Labeling for 13 C Relaxation Measurements on RNA / B.H. Robinson ; G.P. Drobny
Site-Specific Dynamics in DNA: Theory and Experiment
Nucleic Acid Complexes / R. Kaptein ; R. Boelens ; V.P. Chuprina ; J.A.C. Rullmann ; M. Slijper
NMR and Nucleic Acid-Protein Interactions: The Lac Repressor Operator System / Y. Kyogoku ; C. Kojima ; S.J. Lee ; H. Tochio ; N. Suzuki ; H. Matsuo ; M. Shirakawa
Induced Structural Changesin Protein-DNA Complexes / K.B. Hall
Uses of 13 C- and 15 N-labeled RNA in NMR of RNA-Protein Complexes / F. Rastinejad ; C. Evilia ; P. Lu
Studies of Nucleic Acids and Their Protein Interactions by 19 F NMR / M.A. Keniry ; R.H. Shafer
NMR Studies of Drug-DNA Complexes
Author Index
Subject Index
DNA and RNA Structure / U. Schmitz ; T.L. James
How to Generate Accurate Solution Structures of Double-Helical Nucleic Acid Fragments Using Nuclear Magnetic Resonance and Restrained Molecular Dynamics / B.A. Luxon ; D.G. Gorenstein
Comparison of X-ray and NMR-Determined Nucleic Acid Structures / P.K. Radha ; R. Nibedita ; R.A. Kumar ; R.V. Hosur
General Methods for Adhesion to Animal Cells / E. Rozdzinski ; E. Tuomanen
Adhesion of Microbial Pathogens to Leukocyte Integrins: Methods to Study Ligand Mimicry / R.F. Rest
Association of Bacteria with Human Phagocytes / R. Malaviya ; S.N. Abraham
Interaction of Bacteria with Mast Cells / J. Goldhar
Erythrocytes as Target Cells for Testing Bacterial Adhesins / K.A. Krogfelt
Adhesin-Dependent Isolation and Characterization of Bacteria from Their Natural Environment / L.D. Hazlett
Analysis of Ocular Microbial Adhesion
Identification of Adhesins and Receptors / F.J. Cassels ; H. van Halbeek
Isolation and Structural Characterization of Adhesin Polysaccharide Receptors / N. Sharon ; I. Ofek
Identification of Receptors for Bacterial Lectins by Blotting Techniques / W.A. Petri, Jr. ; R.L. Schnaar
Purification and Characterization of Galactose- and N-Acetylgalactosamine-Specific Adhesin Lectin of Entamoeba histolytica / G. Magnusson ; S.J. Hultgren ; J. Kihlberg
Specificity Mapping of Bacterial Lectins by Inhibition of Hemagglutination Using Deoxy and Deoxyfluoro Analogs of Receptor-Active Saccharides / K.K. Lee ; W.Y. Wong ; H.B. Sheth ; R.S.Hodges ; W. Paranchych ; R.T. Irvin
Use of Synthetic Peptides in Characterization of Microbial Adhesins / A. Prakobphol ; H. Leffler ; S.J. Fisher
Identifying Bacterial Receptor Proteins and Quantifying Strength of Interactions They Mediate
General Methods for Visualization and Data Presentation of Adhesion / S. Knutton
Electron Microscopical Methods in Adhesion / P.A. Manning
Use of Confocal Microscopy in Studying Bacterial Adhesion and Invasion / J.R. Johnson
Epidemiological Considerations in Studies of Microbial Adhesion / M.M. Cowan
Kinetic Analysis of Microbial Adhesion / A.G. Matthysse
Observation and Measurement of Bacterial Adhesion to Plants / W. Agace ; S. Hedges ; R. Lindstedt ; M. Svensson ; C. Svanborg
Strategies for Studying Bacterial Adhesion in Vivo / E.V. Sokurenko ; D.L. Hasty
Assay for Adhesion of Host Cells to Immobilized Bacteria
Molecular Biology of Adhesins / V. Vetter ; J. Hacker
Strategies for Employing Molecular Genetics to Study Tip Adhesins / D.M. Schifferli
Use of Tn phoA and T7 RNA Polymerase to Study Fimbrial Proteins / S.I. Hull ; R.A. Hull
Molecular Cloning of Adhesion Genes / E. Hanski ; G. Fogg ; A. Tovi ; N. Okada ; I. Burstein ; M. Caparon
Molecular Analysis of Streptococcus pyogenes Adhesion
Methods for Study of Adhesion of Selected Bacteria / P.S. Cohen ; D.C. Laux
Bacterial Adhesion to and Penetration of Intestinal Mucus in Vitro / E.A. Dean-Nystrom
Identification of Intestinal Receptors for Enterotoxigenic Escherichia coli / M.S. Donnenberg ; J.P. Nataro
Methods for Studying Adhesion of Diarrheagenic Escherichia coli / D.G. Evans ; D.J. Evans, Jr.
Glutathione: Distribution, Biosynthesis, Metabolism, and Transport / D.P. Jones
Glutathione Distribution in Natural Products: Absorption and Tissue Distribution / L.H. Lash
Intracellular Distribution of Thiols and Disulfides: Assay of Mitochondrial Glutathione Transport / A. Meister
Glutathione Biosynthesis and Its Inhibition / W.W. Wells ; D.P. Xu ; M.P. Washburn
Glutathione: Dehydroascorbate Oxidoreductases / F. Ursini ; M. Maiorino ; R. Brigelius-Floheacute; ; K.D. Aumann ; A. Roveri ; D. Schomburg ; L. Floheacute;
Diversity of Glutathione Peroxidases / D.J. Meyer ; B. Ketterer
Purification of Soluble Human Glutathione S-Transferases / H.J. Forman ; M.M. Shi ; T. Iwamoto ; R.-M. Liu ; T.W. Robinson
Measurement of g-Glutamyl Transpeptidase and g-Glutamylcysteine Synthetase Activities in Cells / T. Kondo ; G.L. Dale ; E. Beutler
Thiol Transport from Human Red Blood Cells / J.A. Fuchs
Glutathione Mutants / D.J. Reed
Cystathionine / T. Meier ; R.D. Issels
Promotion of Cyst(e)ine Uptake
Signal Transduction and Gene Regulation / T.P. Holler ; P.B. Hopkins
Ovothiols / S.R. Hubbard
X-Ray Absorption Spectroscopy Studies of Protein Kinase / C. R. Gopalakrishna ; Z.H. Chen ; U. Gundimeda
Modificiations of Cysteine-Rich Regions in Protein Kinase C Induced by Oxidant Tumor Promoters and Enzyme-Specific Inhibitors / F.J. Romero ; J. Romaacute;
Glutathione and Protein Kinase C in Peripheral Nervous Tissues / A.F.G. Quest ; E.S.G. Bardes ; W.Q. Xie ; E. Willott ; R.A. Borchardt ; R.M. Bell
Expression of Protein Kinase Cg Regulatory Domain Elements Containing Cysteine-Rich Zinc-Coordinating Regions as Glutathione S-Transferase Fusion Proteins / F.J.T. Staal ; M.T. Anderson ; L.A. Herzenberg
Redox Regulation of Activation of NF-kB Transcription Factor Complex: Effects of N-Acetycysteine / Y.J. Suzuki ; L. Packer
Redox Regulation of DNA-Protein Interaction by Biothiols / M. Mizuno
Suppression of Protooncogene C-fos Expression by Antioxidant Dihydrolipoic Acid / M.S. Patel ; N.N. Vettakkoruma-Kankav ; T.-C. Liu
Dihydrolipoamide Dehydrogenase: Activity Assays
Thioredoxin and Glutaredoxin / A. Holmgren ; M. Bjouml;rnstedt ; S. Kumar
Selenite and Selenodiglutathione: Reactions with Thioredoxin Systems / R.S. Hutchison ; D.R. Ort
Measurement of Equilibrium Midpoint Potentials of Thiol/Disulfide Regulatory Groups on Thioredoxin-Activated Chloroplast Enzymes / J.H. Wong ; K. Kobrehel ; B.B. Buchanan
Thioredoxin and Seed Proteins / J.-P. Jacquot ; E. Issakidis ; P. Decottignies ; M. Lemaire ; M.-M. Myroslawa
Analysis and Manipulation of Target Enzymes for Thioredoxin Control / K. Schulze-Osthoff ; H. Schenk ; W. Drouml;ge
Effects of Thioredoxin on Activation Transcription Factor NF-KB / M. Russel
Thioredoxin Genetics / P. Schuuml;rmann
Ferredoxin: Thioredoxin System / F. Aring;slund
Flutaredoxin / H.J. Dyson
Nuclear Magnetic Resonance of Thioredoxin and Glutaredoxin
Mimics of Biological Thiols and Thiol Inhibitors / R.S. Glass ; T.C. Stadtman
Selenophosphate / M.-O. Christen
Anethole Dithiolethione: Biochemical Conside
Glutathione: Distribution, Biosynthesis, Metabolism, and Transport / D.P. Jones
Glutathione Distribution in Natural Products: Absorption and Tissue Distribution / L.H. Lash
Intracellular Distribution of Thiols and Disulfides: Assay of Mitochondrial Glutathione Transport / A. Meister
Expression, Purification, and Posttranslational Modification / A.J. Self ; A. Hall
Purification of Recombinant Rho/Rac/G25K fromEscherichia coli / R.A.Cerione ; D. Leonard ; Y. Zheng
Purification of Baculovirus-Expressed Cdc42Hs / T. Mizuno ; H. Nakanishi ; Y. Takai
Purification and Properties of Rac2 from Human Leukemia Cells / U.G. Knaus ; G.M. Bokoch
Purification of Rac2 from Human Neutrophils / A. Abo
Purification of Rac(GDI Dissociation Inhibitor Complex from Phagocyte Cytosol / K. Tanaka ; T. Sasaki
Purification and Properties of Recombinant Rho(GDP Dissociation Inhibitor / M.R. Philips ; M.H. Pillinger
Prenylcysteine-Directed Carboxyl Methyltransferase Activity in Human Neutrophil Membranes
Guanine Nucleotide Exchange and Hydrolysis
Measurement of Intrinsic Nucleotide Exchange and GTP Hydrolysis Rates / M.J. Hart ; R.A. Cerione
Guanine Nucleotide Exchange Catalyzed by dbl Oncogene Product / E. Porfiri ; J.F. Hancock
Stimulation of Nucleotide Exchange on Ras- and Rho-Related Proteins by Small GTP-Binding Protein GDP Dissociation Stimulator / T. Miki
Interaction of Ect2 and Dblwith Rho-related GTPases / D.A. Leonard
Solubilization of Cdc42Hs from Membranes by Rho(GDP Dissociation Inhibitor / J. Settleman ; R. Foster
Purification and GTPase-Activating Protein Activity of Baculovirus Expressed p190 / S. Ahmed ; R. Kozma ; C. Hall ; L. Lim
GTPase-Activating Protein Activity of n(alpha1)-Chimaerin and Effect of Lipids / D.E.H. Afar ; O.N. Witte
Characterization of Breakdown Cluster Region Kinase and SH2-Binding Activities / E. Manser ; T. Leung
Identification of GTPase-Activating Proteins by Nitrocellulose Overlay Assay / P. Cicchetti ; D. Baltimore
Identification of 3BP-1 in cDNA Expression Library by SH3 Domain Screening
Cell Expression and in Vitro Analysis / P. Fort ; S. Vincent
Serum Induction of RhoG Expression / H. Paterson ; P. Adamson ; D. Robertson
Microinjection of Epitope-Tagged Rho Family cDNAs and Analysis by Immunolabeling / S.T. Dillon ; L.A.Feig
Purification and Assay of Recombinant C3 Transferase / K. Aktories ; I. Just
In Vitro ADP-Ribosylation of Rho by Bacterial ADP-Ribosyltransferases / N. Morii ; S. Narumiya
Preparation of Native and Recombinant Clostridium botulinum C3 ADP-Ribosyltransferase and Identification of Rho Proteins by ADP-Ribosylation / D. Diekmann
In Vitro Binding Assay for Interactions of Rho and Rac with GTPase-Activating Proteins and Effectors
Purification and Assay of Kinases That Interact with Rac/Cdc42 / P. Aspenstrim ; M.F. Olson
Yeast Two-Hybrid System to Detect Protein(Protein Interactions with Rho GTPases / S.E. Rittenhouse
Assay for Rho-Dependent Phosphoinositide 3-Kinase Activity in Platelet Cytosol / E.P. Bowman ; D.J. Uhlinger ; J.D. Lambeth
Neutrophil Phospholipase D: Inhibition by Rho(GDP Dissociation Inhibitor and Stimulation by Small GTPase GDP Dissociation Stimulator / M.T. Quinn
Measurement of Rac Translocation from Cytosol to Membranes in Activated Neutrophils / A.W. Segal
Reconst
Expression, Purification, and Posttranslational Modification / A.J. Self ; A. Hall
Purification of Recombinant Rho/Rac/G25K fromEscherichia coli / R.A.Cerione ; D. Leonard ; Y. Zheng
Purification of Baculovirus-Expressed Cdc42Hs / T. Mizuno ; H. Nakanishi ; Y. Takai
Isolation and Utilization of Epidermal Keratinocytes for Oncogene Research / J. Ray ; H.K. Raymon ; F.H. Gage
Generation and Culturing of Precursor Cells and Neuroblasts from Embryonic and Adult Central Nervous System / H. Land
Transformation of Primary Rat Embryo Cells / H. Beug ; P. Steinlein ; P. Bartunek ; M.J. Hayman
Avian Hematopoietic Cell Culture: In Vitro Model Systems to Study Oncogenic Transformation of Hematopoietic Cells / P. Crisanti ; B. Pessac ; G. Calothy
Avian Neuroretina Cells in Oncogene Studies / D. Boettiger ; C. DiLullo
Myogenic and Chondrogenic Cells / W. Krek ; J.A. DeCaprio
Cell Synchronication / T.D. Tlsty ; A. Briot ; B. Poulose
Analysis of Cell Cycle Checkpoint Status in Mammalian Cells / A.M. Killary ; R.E.K. Fournier
Microcell Fusion / M.P. Moyer
Tumor Cells Culture
Molecular Clones / T. Yamamoto ; Y. Kadowaki
Superfamilies of Protooncogenes: Homology Cloning and Characterization of Related Members / M.A. Sells ; J. Chernoff
PCR Cloning of Related Genes / T. Miki ; S.A. Aaronson
Isolation of Oncogenes by Expression cDNA Cloning / J. Samarut ; F. Flamant ; J.-M. Heard
Replication-Competent and -Defective Retrovirus Vectors for Oncogenic Studies / E.M. Blackwood ; R.N. Eisenman
Identification of Protein-Protein Interactions by lambdagt11Expression Cloning / P.L. Bartel ; S. Fields
Analyzing Protein-Protein Interactions Using Two-Hybrid System / G.G. Hicks ; E.-g. Shi ; J. Chen ; M. Roshon ; D. Williamson ; C. Scherer ; H.E. Ruley
Retrovirus Gene Traps / M. Perucho ; J.Welsh ; M.A. Peinado ; Y. Ionov ; M. McClelland
Fingerprinting of DNA and RNA by Arbitrarily Primed PCR: Applications in Cancer Research / N. Lisitsyn ; M. Wigler
Representational Difference Analysis in Detection of Genetic Lesions in Cancer / P. Liang ; D. Bauer ; L. Averboukh ; P. Warthoe ; M. Rohrwild ; M. Strauss ; A.B. Pardee
Analysis of Altered Gene Expression by Differential Display / S.A. Schichman ; C.M. Croce
Approaches to the Identification and Molecular Cloning of Chromosome Breakpoints / P. Lichter ; M. Bentz ; S. Joos
Detection of Chromosomal Aberrations by Means of Molecular Cytogenetics: Painting of Chromosomes and Chromosomal Subregions and Comparative Genomic Hybridization
Gene Function / C. Robinson-Benion ; J.T. Holt
Antisense Techniques / M.A. Labow
Use of lac Activator Proteins for Regulated Expression of Oncogenes / C. Cepko ; E.F. Ryder ; C.P. Austin ; C. Walsh ; D.M. Fekete
Lineage Analysis Using Retrovirus Vectors / L. Tessarollo ; L.F. Parada
Precursors of Quinone-Tanning: Dopa-Containing Proteins / J.H. Waite
Isolation of 2,4,5-Trihydroxyphenylalanine Quinone (Topa Quinone) from Copper Amine Oxidases / S.M. Janes ; J.P. Klinman
Spectrophotometric Detection of Topa Quinone / M. Palcic
Model Studies of Topa Quinone: Synthesis and Characterization of Topa Quinone Derivatives / M. Mure
Catalytic Aerobic Deamination of Activated Primary Amines by a Model for the Quinone Cofactor of Mammalian Copper Amine Oxidases / L.M. Sayre ; Y. Lee
Detection of Reaction Intermediates in Topa Quinone Enzymes / C. Hartmann ; D.M. Dooley
Mass Spectrometric Studies of the Primary Sequence and Structure of Bovine Liver and Serum Amine Oxidase / G.W. Adams ; P. Mayer ; K.F. Medzihradszky ; A.L. Burlingame
Cloning of Mammalian Topa Quinone-Containing Enzymes / D. Mu
Isolation of Active Site Peptides of Lysyl Oxidase / H.M. Kagan ; P. Cai
Resonance Raman Spectroscopy of Quinoproteins / D.E. Brown
Redox-Cycling Detection of Dialyzable Pyrroloquinoline Quinone (PQQ) and Quinoproteins / R. Flackiger ; M.A. Paz ; P.M. Gallop
TryptophanTryptophylquinone (TTQ) in Bacterial Amine Dehydrogenases / W.S. McIntire
Model Studies of Cofactor Tryptophan Tryptophylquinone (TTQ) / S. Itoh ; Y. Ohshiro
Detection of Intermediates in Tryptophan Tryptophylquinone (TTQ) Enzymes / V.L. Davidson ; H.B. Brooks ; M.E. Graichen ; L.H. Jones ; Y.-L. Hyun
X-Ray Studies of Quinoproteins / F.S. Mathews
Genetics of Bacterial Quinoproteins / M.E. Lidstrom
X-Ray Crystallographic Studies of Cofactors in Galactose Oxidase / N. Ito ; P.F. Knowles ; S.E.V. Phillips
Spectroscopic Studies of Galactose Oxidase / J.W. Whittaker
Use of Rapid Kinetics Methods to Study the Assembly of the Diferric-Tyrosyl Radical Cofactor of E. coli Ribonucleotide Reductase / J.M. Bollinger, Jr. ; W.H. Tong ; N. Ravi ; B.H. Huynh ; D.E. Edmondson ; J. Stubbe
Tyrosyl Radicals in Photosystem II / B.A. Barry
Role of Tryptophans in Substrate Binding and Catalysis by DNA Photolyase / S.-T. Kim ; P.F. Heelis ; A. Sancar
Glycyl Free Radical in Pyruvate Formate-Lyase: Synthesis, Structure Characteristics, and Involvementin Catalysis / J. Knappe ; A.F.V. Wagner
Characterization of a Radical Intermediate in the Lysine 2,3-Aminomutase Reaction / G.H. Reed ; M.D. Ballinger
Role of Oxidized Amino Acids in Protein Breakdown and Stability / E.R. Stadtman
Author Index
Subject Index
Precursors of Quinone-Tanning: Dopa-Containing Proteins / J.H. Waite
Isolation of 2,4,5-Trihydroxyphenylalanine Quinone (Topa Quinone) from Copper Amine Oxidases / S.M. Janes ; J.P. Klinman
Spectrophotometric Detection of Topa Quinone / M. Palcic
Expression, Purification, and Posttranslational Modification / C. Nuoffer ; F. Peter ; W.E. Balch
Purification of His6-Tagged Rab1 Proteins Using Bacterial and Insect Cell Expression Systems / H. Horiuchi ; O. Ullrich ; C. Bucci ; M. Zerial
Purification of Posttranslationally Modified and Unmodified Rab5 Protein Expressed in Spodoptera frugiperda Cells / M.A. Riederer ; T. Soldati ; A.B. Dirac-Svejstrup ; S.R. Pfeffer
Expression of Rab9 Protein in Escherichia coli: Purification and Isoprenylation in Vitro / Y. Jiang ; G. Rossi ; S. Ferro-Novick
Characterization of Yeast Type-II Geranylgeranyltransferase / S.A. Armstrong ; M.S. Brown ; J.L. Goldstein ; M.C. Seabra
Preparation of Recombinant Rab Geranylgeranyltransferase and Rab Escort Proteins / K. Kimura ; T. Oka ; A. Nakano
Purification and Assay of Yeast Sar1p / T. Rowe
Expression and Purification of Mammalian Sar1
Guanine NucleotideExchange and Hydrolysis / A. Kikuchi ; H. Nakanishi ; Y. Takai
Purification and Properties of Rab3A / T. Sasaki
Purification and Properties of Bovine Rab-GDP Dissociation Inhibitor / I. Schalk
Expression and Purification of Recombinant His6-Tagged Guanine Nucleotide Dissociation Inhibitor and Formation of Rab1 Complex / D.M. Roberts ; M.D. Garrett ; P.J. Novick
Purification of GDP Dissociation Stimulator Dss4 from Recombinant Bacteria / J.L. Burton ; P. De Camilli
Expression, Purification, and Functional Assay of Mss4 / C. Barlowe ; R. Schekman
Expression, Purification, and Assay of Sec12p: A Sar1p-Specific GDP Dissociation Stimulator / I.G. Macara ; W.H. Brondyk
Oligonucleotide Mutagenesis of Rab GTPases / P. Vollmer ; D. Gallwitz
High Expression Cloning, Purification, and Assay of Ypt-GTPase Activating Proteins / P.A. Randazzo ; O. Weiss ; R.A. Kahn
Preparation of Recombinant ADP-Ribosylation Factor / F.R. Bischoff ; H. Ponstingl
Catalysis of Guanine Nucleotide Exchange of Ran by RCC1 and Stimulation of Hydrolysis of Ran-Bound GTP by Ran-GAP1 / T. Yeung ; T. Yoshihisa
Purification of Sec23p-Sec24p Complex. Cell Expression and Analysis in Vivo / H. Stenmark
Expression of Rab GTPase Using Recombinant Vaccinia Viruses / C. Dascher ; E.J. Tisdale
Transient Expression of Small GTPases to Study Protein Transport along Secretory Pathway in Vivo Using the Recombinant T7 Vaccinia Virus System / J.K. VanSlyke ; L. Thomas ; G. Thomas
Preparation of Recombinant Vaccinia Virus for Expression of Small GTPases / L. Martin-Parras
Using Oligonucleotides for Cloning Rab Proteins by PCR
Use of Two-Hybrid System to Identify Rab-Binding Proteins / H. Damke ; M. Gossen ; S. Freundlieb ; H. Bujard ; S.L. Schmid
Tightly Regulated and Inducible Expression of Dominant Interfering Dynamin Mutant in Stably Transformed HeLa Cells / R.W. Holz ; R.A. Senter ; M.D. Uhler
Investigation by Transient Transfection of the Effects on Regulated Exocytosis of Rab3A
Expression, Purification, and Assays of Gdi1p from Recombinant Escherichia coli
Biological Activity
Expression, Purification, and Posttranslational Modification / C. Nuoffer ; F. Peter ; W.E. Balch
Purification of His6-Tagged Rab1 Proteins Using Bacterial and Insect Cell Expression Systems / H. Horiuchi ; O. Ullrich ; C. Bucci ; M. Zerial
Purification of Posttranslationally Modified and Unmodified Rab5 Protein Expressed in Spodoptera frugiperda Cells / M.A. Riederer ; T. Soldati ; A.B. Dirac-Svejstrup ; S.R. Pfeffer
Glycans of glycoconjugates as modulatory and recognition molecules / N. Sharon
NMR studies of the structure and dynamics of carbohydrates in aqueous solution / H. van Halbeek ; S. Sheng
Linkage analysis by mass spectrometry of chemically modified oligo-saccharides from glycosphingolipids and glycoproteins / B. Nilsson
Development of a novel enzyme based glucose sensor / F. Spener et al
Carbohydrate binding at the active site of
Escherichia coli maltodextrin phosphorylase / P. Drueckes et al.
The chitinolytic system of Streptomyces olivaceoviridis / H. Schrempf
Properties and production of the ß-glycosidase from the thermophilic Archaeon Sulfolobus solfataricus expressed in mesophilic hosts / M. Moracci et al.
Contribution of subsites to catalysis and specificity in the extended binding cleft of Bacillus 1,3-1,4-bgr;-D-glucan 4-glucanohydrolases / A. Planas ; C. Malet
Probing of glycosidase active sites through labeling, mutagenesis and kinetic studies / S.G. Withers
Thiooligosaccharides: toys or tools for the studies of glycanases / H. Driguez
Mutational analysis of catalytic mechanism and specificity in amylolytic enzymes / B. Svensson et al.
The structure and function relationship of Schizophyllum commune xylanase A / M.R. Bray ; A.J. Clarke
Protein engineering of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 / L. Dijkhuzien et al.
Oxidation stable amylases for detergents / T.V. Borchert et al.
Electrostatic studies of carbohydrate active enzymes / A. Baptista et al.
Effects of glycosylation on protein folding, stability and solubility. Studies of chemically modified or engineered plant and fungal peroxidases / K.G. Welinder ; J.W. Tams
Modes of action of two Trichoderma reesei cellobiohydrolases / T.T. Teeri et al.
Structural studies on fungal endoglucanases from Humicola insolens / G.J. Davies ; M. Schulein
The catalytic domain of endoglucanase A from Clostridium cellulolyticum belonging to family 5: an agr;/bgr;-barrel enzyme / V. Ducros et al.
Celluosome domains for novel biotechnological application / E.A. Bayer et al.
Interactions of cellulases from Cellulomonas fimi with cellulose / N. Din et al.
Transgenic plants as a tool to understand starch biosynthesis / J. Kobgr;mann et al.
Targeted expression of microbial cellulases in transgenic animals / S. Ali et al.
Mechanism and action of glucansucrases / J.F. Robyt
Studies of recombinant amylosucrase / M. Remaud-Simeon et al.
Application of cloned monocomponent carbohydrates for modification of plant materials / L.V. Kofod et al.
Fatty acid esters of ethyl glucoside, a unique class of surfactants / O. Andresen ; O. Kirk
A wide range of carbohydrate modifications by a single microorganism: leuconostoc mesenteroides / W. Soetaert et al.
Glycans of glycoconjugates as modulatory and recognition molecules / N. Sharon
NMR studies of the structure and dynamics of carbohydrates in aqueous solution / H. van Halbeek ; S. Sheng
Linkage analysis by mass spectrometry of chemically modified oligo-saccharides from glycosphingolipids and glycoproteins / B. Nilsson
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