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1.

電子ブック

EB
W. E. Balch, William Edward Balch, Channing J. Der, W. E. Balch, Alan Hall, Channing J. Der
出版情報: Elsevier ScienceDirect Books , Burlington : Elsevier, 2006
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Purification and Biochemical Properties of Rac1,2,3 and the Splice Variant Rac1b
Biochemical Analyses of the Wrch Atypical Rho Family GTPases
Purification of P-Rex1 from neutrophils and nucleotide exchange assay
In vitro guanine nucleotide exchange activity of DHR-2/DOCKER/ CZH2 domains
Biochemical characterization of the Cool (Coned-out-of-Library)/Pix (Pak-interactive exchange factor) proteins
GEF and glucosylation assays on liposome bound Rac
Phosphorylation of RhoGDI by p21-activated kinase 1
Purification of ARAP3 and characterization of GAP activities
Regulation of RhoGAP specificity by phospholipids and prenylation
Purification and activity of the Rho ADP-ribosylating binary C2/C3 toxin
Purification of Tat-C3 exoenzymes
Imaging and photobleach correction of MeroCBD, sensor of endogenous Cdc42 activation
Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts
In Vitro Reconstitution of Cdc42-Mediated Actin Assembly Using Purified Components
Biochemical Analysis of Mammalian Formin Effects on Actin Dynamics
Formin proteins: purification and measurement of effects on actin assembly
Purification and Kinase Assay of PKN
Purification and Enzyme Activity of ACK1
Direct activation of purified phospholipase C epsilon by RhoA studied in reconstituted phospholipid vesicles
Regulation of PLCG isoforms by Rac
Biochemical properties and inhibitors of (N-)WASP
The Use of GFP to localize Rho GTPases in Living Cells
Analysis of the Spatio-Temporal Activation of Rho GTPases using Raichu Probes
Measurement of Activity of Rho GTPases during Mitosis
RNAi inhibition of Rho GTPases
RNA interference techniques to study epithelial cell adhesion and polarity
Nucleofection of primary neurons
Dock180-ELMO Cooperation in Rac Activation
Rho GTPase activation by cell-cell adhesion
Activation of Rap1, Cdc42, and Rac by nectin adhesion system
Analysis of activated GAPs and GEFs in cell lysates
Degradation of RhoA by Smurf1 ubiquitin ligase
Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin
Regulation of superoxide-producing NADPH in non-phagocytic cells
Activation of MEKK1 by Rho GTPases
Activation of Apoptotic JNK Pathway through the Rac1-Binding Scaffold Protein POSH
Quantification of isozyme-specific activation of phospholipase C-G2 by Rac GTPases and phospholipase C-s by Rho GTPases in an intact cell assay system
Activation of Rho and Rac by Wnt/Frizzled signalling
Fluorescent assay of cell permeable C3 transferase activity
Use of TIRF microscopy to visualize actin and microtubules in migrating cells
Inhibition of ROCK by RhoE
Conditional regulation of a ROCK-estrogen receptor fusion protein
Rational design and applications of a Rac GTPase-specific small molecule inhibitor
In vitro assay of primary astrocyte migration as a tool to study Rho GTPase function in cell polarization
Real time centrosome reorientation during fibroblast migration
Lentiviral delivery of RNAi in Hippocampal Neurons
Methods for studying neutrophil Chemtoaxis
Measurement of Epidermal Growth Factor Receptor turnover and effects of Cdc42
Tumor cell migration in three dimensions
Reciprocal regulation of cyclin D1 expression by Rac and Rho
Regulation of ERK signal duration and cyclin D1 expression by Rhodependent stress fiber formation
An in vitro model to study the role of endothelial Rho GTPases during leukocyte transendothelial migration
Analysis of a Mitotic role of Cdc42
Plexin-induced collapse assay in COS cells
Morphological and Biochemical Analysis of Rac1 in Three-Dimensional Epithelial Cell Cultures
Using Three Dimensional Acinar Structures for Molecular and Cell Biological Assays
TC10 and insulin-stimulated glucose transport
GTPases and the control of neuronal polarity
In vitro assembly of filopodia-like bundles
Purification and Biochemical Properties of Rac1,2,3 and the Splice Variant Rac1b
Biochemical Analyses of the Wrch Atypical Rho Family GTPases
Purification of P-Rex1 from neutrophils and nucleotide exchange assay
2.

電子ブック

EB
W. E. Balch, William Edward Balch, Channing J. Der, Alan Hall
出版情報: Elsevier ScienceDirect Books , Burlington : Elsevier, 2006
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Real-time in vitro measurement of Intrinsic and Ras GAP-mediated GTP hydrolysis
Schwann cell preparation from single mouse embryos: Analyses of neurofibromin function in Schwann cells
Regulation of the nucleotide state of oncogenic Ras proteins by nucleoside diphosphate kinase
Measurements of TSC2 GAP activity toward Rheb
Characterization of AND-34 function and signalling
Studying the spatial and temporal regulation of Ras GTPase-activating proteins
Activation of Ras Proteins by Ras Guanine Nucleotide Releasing Protein Family Members
Ras and Rap1 Activation of PLC? lipase activity
Specificity and expression of RalGPS as RalGEFs
Biochemical and biological analyses of Rgr RalGEF oncogene
Analysis of Ras Activation in Living Cells with GFP-RBD
Genetic and pharmacologic analyses of the role of Icmt in Ras membrane association and function
Characterization of the activation of the Rap specific exchange factor epac by cyclic nucleotides
Biochemistry of the Rap-specific guanine nucleotide exchange factors PDZ-GEF1 and 2
Characterization of interactions between Ras family GTPases and their effectors
Genetic and pharmacologic dissection of Ras effector utilization in oncogenesis
Sequencing analysis of BRAF mutations in human cancers
KSR regulation of the Raf-MEK-ERK cascade
Ras-sensitive IMP modulation of the Raf/MEK/ERK cascade through KSR1
Raf Kinase Inhibitor Protein (RKIP) regulation of Raf and MAPK signalling
Harnessing RNAi for Analyses of Ras Signaling and Transformation
The Rac Activator Tiam1and Ras induced Oncogenesis
Phospholipase Ce C+ guanine nucleotide exchange factor activity and activation of Rap1
Nore1 and RASSF1 regulation of cell proliferation and of the MST1/2 kinases
RASSF family proteins and Ras transformation
Ras and the Rain/RasBP1 effector
The RIN family of Ras effectors
RAP1 regulation of RIAM and cell adhesion
Regulation of cell-cell adhesion by Rap1
Effects of Ras signaling on gene expression analyzed by customized microrays
Protein-fragment complementation assays (PCA) in small GTPase research and drug discovery
Ras Up-regulation of Cycloxygenase-2
Regulation of the expression of tropomyosins and actin cytoskeleton by ras-transformation
Regulation of Par-4 by Oncogenic Ras
Using Drosophila and yeast genetics to investigate a role for the RhebGTPase in cell growth
Biochemistry and Biology of ARHI (DIRAS3), An Imprinted Tumor Suppressor Gene Whose Expression Is Lost In Ovarian and Breast Cancer
Gem protein signaling and regulation
Analysis of Rem/RGK Signaling and Biological Activity
Analysis of Rit Signaling and Biological Activity
Characterization of RERG: An estrogen-regulated tumor suppressor gene
Inhibition of transcription factor NF-?B activation by ?B-Ras
Analysis of Rhes activation state and effector function
Rheb activation of mTOR and S6K1 signaling
Use of Retrovirus Expression of Interfering RNA to Determine the Contribution of Activated K-Ras and Ras Effector Expression to Human Tumor Cell Growth
Using inhibitors of prenylation to block localization and transforming activity
Sorafenib (BAY 43-9006), a dual action inhibitor that targets RAF/MEK/ERK pathway in tumor cells and tyrosine kinases VEGFR/PDGFR in tumor vasculature
Yeast screens for inhibitors of Ras-Raf interaction and characterization of MCP inhibitors of Ras-Raf interaction
A tagging-via-substrate technology for genome-wide detection and identification of farnesylated proteins
A genetically defined normal human somatic cell system to study Ras oncogenesis in vivo and in vitro
Analyses of Ras transformation of human thyroid epithelial cells
Use of Ras-transformed human ovarian surface epithelial cells as a model for studying ovarian cancer
Physiological analysis of oncogenesic Kras
Use of conditionally active Ras Fusion Proteins to study Epidermal Growth, Differentiation and Neoplasia
Pancreatic duct epithelial cell isolation and cultivation in two dimensional and three dimensiona
Real-time in vitro measurement of Intrinsic and Ras GAP-mediated GTP hydrolysis
Schwann cell preparation from single mouse embryos: Analyses of neurofibromin function in Schwann cells
Regulation of the nucleotide state of oncogenic Ras proteins by nucleoside diphosphate kinase
3.

電子ブック

EB
Judith L. Campbell, Paul Modrich
出版情報: Elsevier ScienceDirect Books , Burlington : Elsevier, 2006
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Contributors to Volume 408
Volumes in Series
Assays for Determining Lesion Bypass Efficiency and Mutagenicity of Site-Specific DNA Lesions In Vivo / James C. Delaney ; John M. Essigmann1:
Oxidative DNA Glycosylases: Recipes from Cloning to Characterization / Viswanath Bandaru ; Jeffrey O. Blaisdell ; Susan S. Wallace2:
Purification and Characterization of NEIL1 and NEIL2, Members of a Distinct Family of Mammalian DNA Glycosylases for Repair of Oxidized Bases / Tapas K. Hazra ; Sankar Mitra3:
Analysis of Base Excision DNA Repair of the Oxidative Lesion 2-Deoxyribonolactone and the Formation of DNA-Protein Cross-Links / Jung-Suk Sung ; Bruce Demple4:
Isolation and Analyses of MutY Homologs (MYH) / A-Lien Lu-Chang5:
Use of Yeast for Detection of Endogenous Abasic Lesions, Their Source, and Their Repair / Serge Boiteux ; Marie Guillet6:
Activities and Mechanism of DNA Polymerase [beta] / William A. Beard ; Rajendra Prasad ; Samuel H. Wilson7:
Direct Removal of Alkylation Damage from DNA by AlkB and Related DNA Dioxygenases / Barbara Sedgwick ; Peter Robins ; Tomas Lindahl8:
Purification and Characterization of DNA Photolyases / Gwendolyn B. Sancar ; Aziz Sancar9:
Genetic and In Vitro Assays of DNA Deamination / Heather A. Coker ; Hugh D. Morgan ; Svend K. Petersen-Mahrt10:
The Xeroderma Pigmentosum Group C Protein Complex and Ultraviolet-Damaged DNA-Binding Protein: Functional Assays for Damage Recognition Factors Involved in Global Genome Repair / Kaoru Sugasawa11:
Purification and Characterization of Escherichia coli and Human Nucleotide Excision Repair Enzyme Systems / Joyce T. Reardon12:
Assays for Transcription Elongation by RNA Polymerase II Using Oligo(dC)-Tailed Template with Single DNA Damage / Isao Kuraoka ; Kiyoji Tanaka13:
In Vivo Assays for Transcription-Coupled Repair / Graciela Spivak ; Gerd P. Pfeifer ; Philip Hanawalt14:
TFIIH Enzymatic Activities in Transcription and Nucleotide Excision Repair / Jean-Philippe Laine ; Vincent Mocquet ; Jean-Marc Egly15:
An Assay for Studying Ubiquitylation of RNA Polymerase II and Other Proteins in Crude Yeast Extracts / James Reid ; Jesper Q. Svejstrup16:
Analysis of Excision Step in Human DNA Mismatch Repair / Jochen Genschel ; Paul Modrich17:
Characterization of the "Mismatch Repairosome" and Its Role in the Processing of Modified Nucleosides In Vitro / Katja Baerenfaller ; Franziska Fischer ; Josef Jiricny18:
Analysis of DNA Mismatch Repair in Cellular Response to DNA Damage / Liya Gu ; Guo-Min Li19:
Characterization of Escherichia coli Translesion Synthesis Polymerases and Their Accessory Factors / Penny J. Beuning ; Sharotka M. Simon ; Veronica G. Godoy ; Daniel F. Jarosz ; Graham C. Walker20:
Measuring the Fidelity of Translesion DNA Synthesis / Scott D. McCulloch ; Thomas A. Kunkel21:
DNA Polymerases for Translesion DNA Synthesis: Enzyme Purification and Mouse Models for Studying Their Function / Paula L. Fischhaber ; Lisa D. McDaniel ; Errol C. Friedberg22:
Purification and Characterization of Escherichia coli DNA Polymerase V / Katharina Schlacher ; Qingfei Jiang ; Roger Woodgate ; Myron F. Goodman23:
Yeast and Human Translesion DNA Synthesis Polymerases: Expression, Purification, and Biochemical Characterization / Robert E. Johnson ; Louise Prakash ; Satya Prakash24:
Localization of Y-Family Polymerases and the DNA Polymerase Switch in Mammalian Cells / Patricia Kannouche ; Alan Lehmann25:
Repair of DNA Double Strand Breaks: In Vivo Biochemistry / Neal Sugawara ; James E. Haber26:
Assays for Nonhomologous End Joining in Extracts / Joe Budman ; Gilbert Chu27:
Purification and Assays of Saccharomyces cerevisiae Homologous Recombination Proteins / Stephen Van Komen ; Margaret Macris ; Michael G. Sehorn ; Patrick Sung28:
Analysis of DNA Recombination and Repair Proteins in Living Cells by Photobleaching Microscopy / Jeroen Essers ; Adriaan B. Houtsmuller ; Roland Kanaar29:
Synthetic Junctions as Tools to Identify and Characterize Holliday Junction Resolvases / Ulrich Rass ; Stephen C. West30:
In Vitro Nonhomologous DNA End Joining System / Yunmei Ma ; Michael R. Lieber31:
RAG and HMGB1 Proteins: Purification and Biochemical Analysis of Recombination Signal Complexes / Serge Bergeron ; Dirk K. Anderson ; Patrick C. Swanson32:
Purification and Biochemical Characterization of Ataxia-Telangiectasia Mutated and Mre11/Rad50/Nbs1 / Ji-Hoon Lee ; Tanya T. Paull33:
Author Index
Subject Index
Contributors to Volume 408
Volumes in Series
Assays for Determining Lesion Bypass Efficiency and Mutagenicity of Site-Specific DNA Lesions In Vivo / James C. Delaney ; John M. Essigmann1:
4.

電子ブック

EB
Judith L. Campbell, Judith L. Campbell, Paul Modrich
出版情報: Elsevier ScienceDirect Books , Burlington : Elsevier, 2006
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Contributors to Volume 409
Volumes in Series
Overproduction and Purification of RFC-Related Clamp Loaders and PCNA-Related Clamps from Saccharomyces cerevisiae / Goran O. Bylund ; Jerzy Majka ; Peter M. J. Burgers1:
Functional Assays for Replication Protein A (RPA) / Sara K. Binz ; Anne M. Dickson ; Stuart J. Haring ; Marc S. Wold2:
Human DNA Ligases I, III, and IV-Purification and New Specific Assays for These Enzymes / Xi Chen ; John Pascal ; Sangeetha Vijayakumar ; Gerald M. Wilson ; Tom Ellenberger ; Alan E. Tomkinson3:
Enzymatic Mechanism of the WRN Helicase/Nuclease / Robert M. Brosh, Jr. ; Patricia L. Opresko ; Vilhelm A. Bohr4:
Analysis of the DNA Unwinding Activity of RecQ Family Helicases / Csanad Z. Bachrati ; Ian D. Hickson5:
Characterization of Checkpoint Responses to DNA Damage in Saccharomyces cerevisiae: Basic Protocols / Ritu Pabla ; Vaibhav Pawar ; Hong Zhang ; Wolfram Siede6:
Recruitment of ATR-ATRIP, Rad17, and 9-1-1 Complexes to DNA Damage / Xiaohong Helena Yang ; Lee Zou7:
Multiple Approaches to Study S. cerevisiae Rad9, a Prototypical Checkpoint Protein / Aisling M. O'Shaughnessy ; Muriel Grenon ; Chris Gilbert ; Geraldine W.-L. Toh ; Catherine M. Green ; Noel F. Lowndes8:
Methods for Studying Adaptation to the DNA Damage Checkpoint in Yeast / David P. Toczyski9:
DNA Damage-Induced Phosphorylation of Rad55 Protein as a Sentinel for DNA Damage Checkpoint Activation in S. cervisiae / Vladimir I. Bashkirov ; Kristina Herzberg ; Edwin Haghnazari ; Alexey S. Vlasenko ; Wolf-Dietrich Heyer10:
Methods for Studying Mutagenesis and Checkpoints in Schizosaccharomyces pombe / Mihoko Kai ; Lorena Taricani ; Teresa S.-F. Wang11:
Methods for Determining Spontaneous Mutation Rates / Patricia L. Foster12:
Genomic Approaches for Identifying DNA Damage Response Pathways in S. cerevisiae / Michael Chang ; Ainslie B. Parsons ; Bilal H. Sheikh ; Charles Boone ; Grant W. Brown13:
Techniques for [gamma]-H2AX Detection / Asako Nakamura ; Olga A. Sedelnikova ; Christophe Redon ; Duane R. Pilch ; Natasha I. Sinogeeva ; Robert Shroff ; Michael Lichten ; William M. Bonner14:
Methods for Studying the Cellular Response to DNA Damage: Influence of the Mre11 Complex on Chromosome Metabolism / Jan-Willem F. Theunissen ; John H. J. Petrini15:
Detecting Repair Intermediates In Vivo: Effects of DNA Damage Response Genes on Single-Stranded DNA Accumulation at Uncapped Telomeres in Budding Yeast / Mikhaljlo K. Zubko ; Laura Maringele ; Steven S. Foster ; David Lydall16:
Analysis of Non-B DNA Structure at Chromosomal Sites in the Mammalian Genome / Sathees C. Raghavan ; Albert Tsai ; Chih-Lin Hsieh ; Michael R. Lieber17:
Detection and Structural Analysis of R-Loops / Kefei Yu ; Deepankar Roy ; Feng-Ting Huang18:
The Delitto Perfetto Approach to In Vivo Site-Directed Mutagenesis and Chromosome Rearrangements with Synthetic Oligonucleotides in Yeast / Francesca Storici ; Michael A. Resnick19:
Assays for Transcriptional Mutagenesis in Active Genes / Damien Bregeon ; Paul W. Doetsch20:
Methods for Studying Chromatin Assembly Coupled to DNA Repair / Annabelle Gerard ; Sophie E. Polo ; Daniele Roche ; Genevieve Almouzni21:
Structure-Function Analysis of SWI2/SNF2 Enzymes / Harald Durr ; Karl-Peter Hopfner22:
Genomic Approach for the Understanding of Dynamic Aspect of Chromosome Behavior / Yuki Katou ; Kiyofumi Kaneshiro ; Hiroyuki Aburatani ; Katsuhiko Shirahige23:
Measurement of Chromosomal DNA Single-Strand Breaks and Replication Fork Progression Rates / Claire Breslin ; Paula M. Clements ; Sherif F. El-Khamisy ; Eva Petermann ; Natasha Iles ; Keith W. Caldecott24:
Monitoring DNA Replication Following UV-Induced Damage in Escherichia coli / Charmain T. Courcelle ; Justin Courcelle25:
Methods to Study Replication Fork Collapse in Budding Yeast / Giordano Liberi ; Cecilia Cotta-Ramusino ; Massimo Lopes ; Jose' Sogo ; Chiara Conti ; Aaron Bensimon ; Marco Foiani26:
Analysis of Gross-Chromosomal Rearrangements in Saccharomyces cerevisiae / Kristina H. Schmidt ; Vincent Pennaneach ; Christopher D. Putnam ; Richard D. Kolodner27:
Formation and Processing of Stalled Replication Forks-Utility of Two-Dimensional Agarose Gels / Jennifer Reineke Pohlhaus ; Kenneth N. Kreuzer28:
Poly (ADP-Ribose) Polymerase-1 Activation During DNA Damage and Repair / Francoise Dantzer ; Jean-Christopher Ame ; Valerie Schreiber ; Jun Nakamura ; Josiane Menissier-de Murcia ; Gilbert de Murcia29:
Tyrosyl-DNA Phosphodiesterase (Tdp1) (3'-Phosphotyrosyl DNA Phosphodiesterase) / Amy C. Raymond ; Alex B. Burgin, Jr.30:
Assaying Double-Strand Break Repair Pathway Choice in Mammalian Cells Using a Targeted Endonuclease or the RAG Recombinase / David M. Weinstock ; Koji Nakanishi ; Hildur R. Helgadottir ; Maria Jasin31:
Author Index
Subject Index
Contributors to Volume 409
Volumes in Series
Overproduction and Purification of RFC-Related Clamp Loaders and PCNA-Related Clamps from Saccharomyces cerevisiae / Goran O. Bylund ; Jerzy Majka ; Peter M. J. Burgers1:
5.

電子ブック

EB
Kwang W. Jeon, Alan R. Kimmel, Brian Oliver
出版情報: Elsevier ScienceDirect Books , Burlington : Elsevier, 2006
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Contributors to Volume 410
Volumes in Series
Array Platforms / Section I:
The Affymetrix GeneChip Platform: An Overview / Dennise D. ; Dalma-Weiszhausz ; Janet Warrington ; Eugene Y. Tanimoto ; C. Garrett Miyada1:
The Agilent In Situ-Synthesized Microarray Platform / Paul K. Wolber ; Patrick J. Collins ; Anne B. Lucas ; Anniek De Witte ; Karen W. Shannon2:
Illumina Universal Bead Arrays / Jian-Bing Fan ; Kevin L. Gunderson ; Marina Bibikova ; Joanne M. Yeakley ; Jing Chen ; Eliza Wickham Garcia ; Lori L. Lebruska ; Marc Laurent ; Richard Shen ; David Barker3:
Microarray Oligonucleotide Probes / David P. Kreil ; Roslin R. Russell ; Steven Russell4:
Automated Liquid Handling and High-Throughput Preparation of Polymerase Chain Reaction-Amplified DNA for Microarray Fabrication / Angela Burr ; Kevin Bogart ; Jason Conaty ; Justen Andrews5:
The Printing Process: Tips on Tips / Reed A. George6:
Making and Using Spotted DNA Microarrays in an Academic Core Laboratory / Janet Hager7:
Printing Your Own Inkjet Microarrays / Christopher G. Lausted ; Charles B. Warren ; Leroy E. Hood ; Stephen R. Lasky8:
Peptide Nucleic Acid Microarrays Made with (S,S)-trans-Cyclopentane-Constrained Peptide Nucleic Acids / Mark A. Witschi ; Jonathan K. Pokorski ; Daniel H. Appella9:
Wet-Bench Protocols / Section II:
Optimizing Experiment and Analysis Parameters for Spotted Microarrays / Scott J. Neal ; J. Timothy Westwood10:
Sample Labeling: An Overview / Michael Brownstein11:
Genomic DNA as a General Cohybridization Standard for Ratiometric Microarrays / Brian A. Williams ; Richele M. Gwirtz ; Barbara J. Wold12:
Analysis of Sequence Specificities of DNA-Binding Proteins with Protein Binding Microarrays / Martha L. Bulyk13:
Microarray Analysis of RNA Processing and Modification / Timothy R. Hughes ; Shawna L. Hiley ; Arneet L. Saltzman ; Tomas Babak ; Benjamin J. Blencowe14:
Mapping the Distribution of Chromatin Proteins by ChIP on Chip / Nicolas Negre ; Sergey Lavrov ; Jerome Hennetin ; Michel Bellis ; Giacomo Cavalli15:
DamID: Mapping of In Vivo Protein-Genome Interactions Using Tethered DNA Adenine Methyltransferase / Frauke Greil ; Celine Moorman ; Bas van Steensel16:
Whole-Genome Genotyping / Frank J. Steemers ; Hongi Ren ; Pauline Ng ; Lixin Zhou ; Chan Tsan ; Weihua Chang ; Dave Bullis ; Joe Musmacker ; Christine King ; Arnold Oliphant ; Kenneth M. Kuhn17:
Mapping Drosophila Genomic Aberration Breakpoints with Comparative Genome Hybridization on Microarrays / Jeremy N. Erickson ; Eric P. Spana18:
Performing Quantitative Reverse-Transcribed Polymerase Chain Reaction Experiments / Georges Lutfalla ; Gilles Uze19:
The Application of Tissue Microarrays in the Validation of Microarray Results / Stephen M. Hewitt20:
Mapping Histone Modifications by Nucleosome Immunoprecipitation / David J. Clark ; Chang-Hui Shen21:
Author Index
Subject Index
Contributors to Volume 410
Volumes in Series
Array Platforms / Section I:
6.

電子ブック

EB
Kwang W. Jeon, Alan R. Kimmel, Brian Oliver
出版情報: Elsevier ScienceDirect Books , Burlington : Elsevier, 2006
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Databases and statistics / Section I:
The Use of External Controls in Microarray Experiments / Chapter 1:
Standards in gene expression microarray experiments / Chapter 2:
Scanning Microarrays: Current Methods and Future Directions / Chapter 3:
BioArray software environment / Chapter 4:
Bioconductor: An open source framework for bioinformatics and computational biology / Chapter 5:
TM4 microarray software suite / Chapter 6:
Clustering microarray data / Chapter 7:
Analysis of variance of microarray data / Chapter 8:
Microarray quality control / Chapter 9:
Principle component and ANOVA analysis of array data using Partekreg; Genomics SolutionTM / Chapter 10:
Statistics for ChIP-chip and DNase hypersensitivity experiments on NimbleGen arrays / Chapter 11:
Extrapolating traditional DNA microarray statistics to the tiling and protein microarray technologies / Chapter 12:
Random dataset generation to support microarray analysis / Chapter 13:
Using ontologies to annotate microarray experiments / Chapter 14:
Interpreting experimental results using gene ontologies / Chapter 15:
Gene Expression Omnibus (GEO): Microarray data storage, submission, retrieval and analysis / Chapter 16:
Data storage and analysis in ArrayExpress / Chapter 17:
Clustering methods for analyzing large datasets: gonad development, a study case / Chapter 18:
GeneSprings / Chapter 19:
Visualizing networks / Chapter 20:
Random forests for array data / Chapter 21:
Hybridization troubleshooting / Chapter 22:
RNA extraction and handling for microarray analysis / Chapter 23:
Analyzing MicroRNA expression using microarrays / Chapter 24:
Databases and statistics / Section I:
The Use of External Controls in Microarray Experiments / Chapter 1:
Standards in gene expression microarray experiments / Chapter 2:
7.

電子ブック

EB
Indu Kheterpal, Ronald Wetzel
出版情報: Elsevier ScienceDirect Books , Burlington : Elsevier, 2006
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Characterization of protein deposition in vivo and ex vivo
PMCA for diagnosis and prion propagation studies / Chapter 1:
Fractionation of prion protein aggregates by asymmetrical flow field-flow fractionation / Chapter 2:
Analysis of Amyloid Aggregates Using Agarose Gel Electrophoresis / Chapter 3:
Characterization of Systemic Amyloid Deposits by Mass Spectrometry / Chapter 4:
Proteomics of Polyglutamine Aggregates / Chapter 5:
Merger of Laser Capture Microdissection and Mass Spectrometry: A Window into the Amyloid Plaque Proteome / Chapter 6:
MALDI MS Imaging of Amyloid / Chapter 7:
Imaging polyglutamine deposits in brain tissue / Chapter 8:
X-34 labeling of abnormal protein aggregates during the progression of Alzheimer's disease / Chapter 9:
Visualizing Pathology Deposits in the Living Brain of Alzheimer's Disease Patients / Chapter 10:
Micro-Imaging of Amyloid in Mice / Chapter 11:
Cell and animal models of amyloid formation and toxicity
An efficient protein transformation protocol for introducing prions into yeast / Chapter 12:
Screening for genetic modifiers of amyloid toxicity in yeast / Chapter 13:
Searching for anti-prion compounds: Cell-based high-throughput in vitro assays and animal testing strategies / Chapter 14:
A Drosophila Model of Alzheimer's Disease / Chapter 15:
A C. elegans model of polyglutamine disease / Chapter 16:
Computational Approaches and Theory
Nucleation: the Connections between Equilibrium and Kinetic Behavior / Chapter 17:
Computational Approaches to Amyloid beta Fibril Core Structure / Chapter 18:
Amyloid beta-protein aggregations: Ab initio discrete molecular dynamics approaches / Chapter 19:
Computational approaches to fibril structure and formation / Chapter 20:
Characterization of protein deposition in vivo and ex vivo
PMCA for diagnosis and prion propagation studies / Chapter 1:
Fractionation of prion protein aggregates by asymmetrical flow field-flow fractionation / Chapter 2:
8.

電子ブック

EB
Indu Kheterpal, Ronald Wetzel
出版情報: Elsevier ScienceDirect Books , Burlington : Elsevier, 2006
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Characterization of protein deposition in vitro
Purification of Polyglutamine Proteins / Chapter 1:
Preparation of amyloid beta-protein for structural and functional studies / Chapter 2:
Kinetics and thermodynamics of amyloid assembly using an HPLC-based sedimentation assay / Chapter 3:
Protein aggregation starting from the native, globular stete / Chapter 4:
Direct observation of amyloid growth monitored by total internal reflection fluorescence microscopy / Chapter 5:
Characterization of Amyloid Structures at the Molecular Level by Solid State Nuclear Magnetic Resonance Spectroscopy / Chapter 6:
Spin Labeling Analysis of Amyloids and other Protein Aggregates / Chapter 7:
Hydrogen-Deuterium Exchange Mass Spectrometry of Protein Aggregates / Chapter 8:
Hydrogen-Deuterium Exchange analyzed by Matrix Assisted Laser Desorption-Ionisation Mass / Chapter 9:
Spectrometry and the HET-s prion model
Analysis of amyloid fibril structure by scanning cysteine mutagenesis / Chapter 10:
Sedimentation velocity analysis of amyloid oligomers and fibrils / Chapter 11:
Structural study of metastable amyloidogenic protein oligomers by Photo-Induced Cross-linking of Unmodified Proteins (PICUP) / Chapter 12:
High Pressure Studies on Protein Aggregates and Amyloid Fibrils / Chapter 13:
Phage Display Screening for Peptides that Inhibit Polyglutamine Aggegation / Chapter 14:
Peptide Based Inhibitors of Amyloid Assembly / Chapter 15:
Screening for Modulators of Aggregation with a Microplate Elongation Assay / Chapter 16:
Conformation-dependent anti-amyloid oligomer antibodies / Chapter 17:
Characterization of protein deposition in vitro
Purification of Polyglutamine Proteins / Chapter 1:
Preparation of amyloid beta-protein for structural and functional studies / Chapter 2:
9.

電子ブック

EB
James Inglese
出版情報: Elsevier ScienceDirect Books , Burlington : Elsevier, 2006
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Dynamic GFP Sensors for High-Content Analysis of the Cell Cycle
High Content Fluorescence-based Screening for Epigenetic Modulators
Development of Assays for Nuclear Receptor Modulators Using Fluorescently Tagged Proteins
The Ligand-Independent Translocation Assay: An Enabling Technology for Screening Orphan GPCRs by Arrestin Recruitment
High Content Screening of Known G Protein-Coupled Receptors by Arrestin Translocation
Cell Imaging Assays for G protein-coupled Receptor Internalization: Application to High Throughput Screening
High throughput confocal microscopy for beta arrestin-GFP translocation GPCR assays using the Evotec Opera
G-protein coupled receptor internalization assays in the High Content Screening Format
Screening for Activators of the Wnt/Fzd Pathway by Automated Fluorescent Microscopy
A live-cell, image based approach to understanding the enzymology and pharmacology of 2-bromopalmitate and palmitoylation
Quantitative single cell studies of nuclear receptor function using high-resolution HTM and image analyses
Tracking the motion of individual proteins with semiconductor quantum dots
Development and Application of Automatic High Resolution Light Microscopy for Cell Based Screens
Adenoviral Sensors for High-Content Cellular Analysis
Cell based assays in primary endothelial cells to study multiple steps in inflammation
Development and Implementation of Multiplexed Cell-Based Imaging Assays
High throughput screening for modulators of stem cell differentiation
High Content Kinetic Calcium Imaging in Drug Sensitive and Drug Resistant Human Breast Cancer Cells
Measurement and Analysis of Calcium Signaling in Heterogeneous Cell Cultures
Mulipliex Analysis of Inflammatory Signalling Pathways using a High Content Imaging System
Generation and Characterization of a Stable MK2-EGFP Cell Line and Subswquent Development of a High Content Imaging Assay on the Cellomics ArrayScan“ Platform to Screen for p38 MAPK Inhibitors
Development and implementation of three MAPK signaling pathway imaging assays to provide MAPK module selectivity profiling for kinase inhibitors; MK2-EGFP translocation, c-Jun & ERK activation
Assay Development and Case History of a 32K Biased Library High Content MK2-EGFP Translocation Screen to Identify p38 MAPK Inhibitors on the ArrayScan“ 3.1 Imaging Platform
Development and Implementation of a dual target MAPK high content assay (MK2-EGFP translocation & cJun activation) to provide LO support for kinase inhibitors
Compound classification using image-based cellular phenotypes
High Content Screening: Emerging Hardware and Software Technologies
An Infrastructure for High Throughput Microscopy: Instrumentation, Informatics, and Integration
Protein Translocation Assays: Key Tools for Accessing New Biological Information with High Throughput Microscopy
High-Content Screening of Functional Genomic Libraries
Fluorescent protein-based cellular assays analyzed by laser scanning microplate cytometry in 1536-well plate format
High-Throughput Measurements of Biochemical Responses Using the Plate::VisionT Multimode 96 Mini-lens Array Reader
Systems Cell Biology Based on High Content Screening
Digital Autofocus Methods for Automated Microscopy
Fluorescence Lifetime Imagine Microscopy - Two-dimensional distribution measurement of fluorescence lifetime
Dynamic GFP Sensors for High-Content Analysis of the Cell Cycle
High Content Fluorescence-based Screening for Epigenetic Modulators
Development of Assays for Nuclear Receptor Modulators Using Fluorescently Tagged Proteins
10.

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EB
Minoru Fukuda
出版情報: Elsevier ScienceDirect Books , Burlington : Elsevier, 2006
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N-Glycan processing Non-radioactive analysis of lipid-linked oligosaccharide compositions by fluorophore-assisted carbohydrate electrophoresis (FACE) / Section 1:
Identification of N-glycan-binding proteins for E3 ubiquitin ligases
Familyi 47 aacute;-mannosidases in N-glycan processing
A Cytoplasmic Peptide: N-glycanase
Structural Analysis Glycomic profiling of cells and tissues by mass spectrometry fingerprinting and sequencing methodologies / Section 2:
Structural analysis of sialyl N-glycan using pyridylamination and chromatography followed by multistage tandem mass spectrometry
Determination of glycosylation sites and disulfide bond structures using LC/ESI-MS/MS analysis
Identification of O-GlcNAc sites on proteins
Carbohydrate Synthesis and Antibiotics Chemoenzymatic synthesis of glycan libraries / Section 3:
Glycoconjugate vaccines against Haemophilus influenzae tybe b
Assay of human gastric mucin as a natural antibiotic against Helicobacter pylori
Molecular contacts between antibiotics and the 30S ribosomal particle
Mechanism-based inhibitors to probe transitional states of glycoside hydrolases
Regulating cell surface glycosylation with a small molecule switch
Metabolic labeling of glycans with azido sugars for visualization and glycoproteomics
Carbohydrate Ligand Specificity Functional Proteomic Profiling of Glycan Processing Enzymes / Section 4:
Oligosaccharide Microarrays to Map Interactions of Carbohydrates in Biological Systems
Identification of Ligand Specificities for Glycan-binding Proteins Using Glycan Arrays
High-throughput analysis of lectin-oligosaccharide interactions by automated frontal affinity chromatography
Preparation of Neoglycolipids with Ring-Closed Cores via Chemoselective Oxime-Ligation for Microarray Analysis of Carbohydrate-Protein Interactions
Development of a lectin microarray based on an evanescent-field fluorescence principle
Introduction to Glycoscience / Volume 1:
Synthesis of Carbohydrate?
Analysis of Glycans / Volume 2:
Polysaccharide Functional Propertie?
Biochemistry of Glycoconjugate Glycans / Volume 3:
Carbohydrate-Mediated Interaction?
Cell Glycobiology and Development / Volume 4:
Health and Disease in Glycomedicine
N-Glycan processing Non-radioactive analysis of lipid-linked oligosaccharide compositions by fluorophore-assisted carbohydrate electrophoresis (FACE) / Section 1:
Identification of N-glycan-binding proteins for E3 ubiquitin ligases
Familyi 47 aacute;-mannosidases in N-glycan processing
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