| Purification and Biochemical Properties of Rac1,2,3 and the Splice Variant Rac1b |
| Biochemical Analyses of the Wrch Atypical Rho Family GTPases |
| Purification of P-Rex1 from neutrophils and nucleotide exchange assay |
| In vitro guanine nucleotide exchange activity of DHR-2/DOCKER/ CZH2 domains |
| Biochemical characterization of the Cool (Coned-out-of-Library)/Pix (Pak-interactive exchange factor) proteins |
| GEF and glucosylation assays on liposome bound Rac |
| Phosphorylation of RhoGDI by p21-activated kinase 1 |
| Purification of ARAP3 and characterization of GAP activities |
| Regulation of RhoGAP specificity by phospholipids and prenylation |
| Purification and activity of the Rho ADP-ribosylating binary C2/C3 toxin |
| Purification of Tat-C3 exoenzymes |
| Imaging and photobleach correction of MeroCBD, sensor of endogenous Cdc42 activation |
| Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts |
| In Vitro Reconstitution of Cdc42-Mediated Actin Assembly Using Purified Components |
| Biochemical Analysis of Mammalian Formin Effects on Actin Dynamics |
| Formin proteins: purification and measurement of effects on actin assembly |
| Purification and Kinase Assay of PKN |
| Purification and Enzyme Activity of ACK1 |
| Direct activation of purified phospholipase C epsilon by RhoA studied in reconstituted phospholipid vesicles |
| Regulation of PLCG isoforms by Rac |
| Biochemical properties and inhibitors of (N-)WASP |
| The Use of GFP to localize Rho GTPases in Living Cells |
| Analysis of the Spatio-Temporal Activation of Rho GTPases using Raichu Probes |
| Measurement of Activity of Rho GTPases during Mitosis |
| RNAi inhibition of Rho GTPases |
| RNA interference techniques to study epithelial cell adhesion and polarity |
| Nucleofection of primary neurons |
| Dock180-ELMO Cooperation in Rac Activation |
| Rho GTPase activation by cell-cell adhesion |
| Activation of Rap1, Cdc42, and Rac by nectin adhesion system |
| Analysis of activated GAPs and GEFs in cell lysates |
| Degradation of RhoA by Smurf1 ubiquitin ligase |
| Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin |
| Regulation of superoxide-producing NADPH in non-phagocytic cells |
| Activation of MEKK1 by Rho GTPases |
| Activation of Apoptotic JNK Pathway through the Rac1-Binding Scaffold Protein POSH |
| Quantification of isozyme-specific activation of phospholipase C-G2 by Rac GTPases and phospholipase C-s by Rho GTPases in an intact cell assay system |
| Activation of Rho and Rac by Wnt/Frizzled signalling |
| Fluorescent assay of cell permeable C3 transferase activity |
| Use of TIRF microscopy to visualize actin and microtubules in migrating cells |
| Inhibition of ROCK by RhoE |
| Conditional regulation of a ROCK-estrogen receptor fusion protein |
| Rational design and applications of a Rac GTPase-specific small molecule inhibitor |
| In vitro assay of primary astrocyte migration as a tool to study Rho GTPase function in cell polarization |
| Real time centrosome reorientation during fibroblast migration |
| Lentiviral delivery of RNAi in Hippocampal Neurons |
| Methods for studying neutrophil Chemtoaxis |
| Measurement of Epidermal Growth Factor Receptor turnover and effects of Cdc42 |
| Tumor cell migration in three dimensions |
| Reciprocal regulation of cyclin D1 expression by Rac and Rho |
| Regulation of ERK signal duration and cyclin D1 expression by Rhodependent stress fiber formation |
| An in vitro model to study the role of endothelial Rho GTPases during leukocyte transendothelial migration |
| Analysis of a Mitotic role of Cdc42 |
| Plexin-induced collapse assay in COS cells |
| Morphological and Biochemical Analysis of Rac1 in Three-Dimensional Epithelial Cell Cultures |
| Using Three Dimensional Acinar Structures for Molecular and Cell Biological Assays |
| TC10 and insulin-stimulated glucose transport |
| GTPases and the control of neuronal polarity |
| In vitro assembly of filopodia-like bundles |
| Purification and Biochemical Properties of Rac1,2,3 and the Splice Variant Rac1b |
| Biochemical Analyses of the Wrch Atypical Rho Family GTPases |
| Purification of P-Rex1 from neutrophils and nucleotide exchange assay |
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