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1.

電子ブック

EB
Damon J. Hamel, Tracy M. Handel
出版情報: Elsevier ScienceDirect Books , 2009
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目次情報: 続きを見る
Structure and Function
A Novel Chemokine-mediated In Vivo T Cell Recruitment Assay for Structure Function Studies / Andrew D. Luster ; Gabriele Campanella1:
Tyrosine sulfation of HIV-1 coreceptors and other chemokine-binding receptors / Micheal R. Farzan2:
Lymphotactin Structural Dynamics / Brian F. Volkman3:
Regulation of chemokines by posttranslational modifications / Paul Proost4:
Proteolytic Processing of Chemokines: Mass Spectrometry, Biochemistry, Neoepitope Antibody and Functional Characterization / Christopher Overall ; Amanda Starr5:
Multiple approaches to study Chemokine Receptor Homo and Heterodimerization / Mario Mellado6:
Heterodimerization of Chemokine Receptors with Other GPCRs / Graeme Milligan ; Shirley Appelbe7:
Homo and Hetero-Oligomerization of Chemokines / Tracy Handel ; Damon Hamel ; Ariane Jansma8:
Interactions of Chemokine with Glycosaminoglycans / Amanda Proudfoot9:
Structures of Chemokine:Chemokine Binding Protein Complexes / Andreas J. Kungl ; Daved Fremont10:
Activation Mechanisms of Chemokine Receptors / Mette Rosenkilde12:
Modeling Small Molecule Compound Binding to Receptors / Richard Horuk ; James Pease ; N. Vaidehi13:
Spectroscopic Approaches to Study Conformational Changes in GPCRs / David Farrens14:
Plasmon Resonance Methods in GPCR Signaling / Victor Hruby ; Isabel Alves ; Gordon Tollin15:
Flow Cytometric Analyses of GPCR Interactions or Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics / Larry Sklar16:
Expression and Functional Reconstitution of CCR1 / Samantha Allen17:
Characterization of the Interactions of Chemokines with Glycosaminoglycans by Fourier Transform Ion Cyclotron Resonance / Julie A. Leary18:
Chemokine Function in Cell Migration
In vitro assays for studying chemokine-triggered lymphocyte transendothelial migration under physiological flow conditions / Ronen Alon ; Francis Lin19:
Microfluidic device / Eugene Butcher20:
Two-photon imaging and quantitative analysis of leukocyte migration in vivo / Mark Miller ; Jenna Cash21:
Zymosan induced peritonitis as a model for studying chemokines and inflammatory mediators in vivo / David Greaves22:
Structure and Function
A Novel Chemokine-mediated In Vivo T Cell Recruitment Assay for Structure Function Studies / Andrew D. Luster ; Gabriele Campanella1:
Tyrosine sulfation of HIV-1 coreceptors and other chemokine-binding receptors / Micheal R. Farzan2:
2.

電子ブック

EB
Damon J. Hamel, Tracy M. Handel
出版情報: Elsevier ScienceDirect Books , 2009
所蔵情報: loading…
目次情報: 続きを見る
Contributors
Preface
Volumes in Series
Chemokines and Receptors in Disease / Section I:
Chemokines in Human Breast Tumor Cells: Modifying Their Expression Levels and Determining Their Effects on the Malignancy Phenotype / Gali Soria ; Tsipi Meshel ; Adit Ben-Baruch1:
Introduction
Modifying Chemokine Expression in Breast Tumor Cells / 2:
Establishment of Primary Local Breast Tumors and Pulmonary Metastases / 3:
Acknowledgments
References
CCR5 Pharmacology Methodologies and Associated Applications / Roy Mansfield ; Sarah Able ; Paul Griffin ; Becky Irvine ; Ian James ; Malcolm Marcartney ; Ken Miller ; James Mills ; Carolyn Napier ; Iva Navratilova ; Manos Perros ; Graham Rickett ; Harriet Root ; Elna van der Ryst ; Mike Westby ; Patrick Dorr
CCR5 Signaling Assays and Application to Quantify and Characterize Ligand-Dependent Agonism, Antagonism, and Inverse Agonism
CCR5-Associated Ligand-Binding Assays
Surrogate In Vitro Antiviral Assays / 4:
CCR5 Site-Directed Mutagenesis and Ligand Docking Studies / 5:
Non-HIV Indications-Associated Studies, Human CCR5 Knock-In Mice / 6:
CXCR4 and Mobilization of Hematopoietic Precursors / Michael P. Rettig ; Pablo Ramirez ; Bruno Nervi ; John F. DiPersio
HSPC Mobilizing Agents that Target the CXCL12/CXCR4 Axis
Donor Selection for HSPC Mobilization
Flow Cytometric Enumeration of Mobilized HSPCs
Dosing and Kinetics of HSPC Mobilization by G-CSF and Plerixafor
Functional Characterization of Mobilized HSPCs / 7:
Concluding Remarks / 8:
Acknowledgment
Double-Label Nonradioactive In Situ Hybridization for the Analysis of Chemokine Receptor Expression in the Central Nervous System / Meizhang Li ; Richard M. Ransohoff
Basic Protocol for ISH (Using Digoxygenin-Labeled Probe)
Comments
Expression of Chemokines and Chemokine Receptors in Human Colon Cancer / Marco Erreni ; Paolo Bianchi ; Luigi Laghi ; Massimiliano Mirolo ; Marco Fabbri ; Massimo Locati ; Alberto Mantovani ; Paola Allavena
Materials and Methods
Results
Discussion
Chemokine Related Proteins from Pathogens / Section II:
Kaposi's Sarcoma Virally Encoded, G-Protein-Coupled Receptor: A Paradigm for Paracrine Transformation / Daniel Martin ; J. Silvio Gutkind
Cloning of vGPCR
Assaying vGPCR Transforming Activity In Vitro
vGPCR Transforming Activity In Vivo Using Xenograft Systems
In Vivo Targeted Infection Using the TVA-RCAS System
vGPCR-Induced Paracrine Transformation
Characterization of vGPCR-Induced Molecular Signaling
Evaluation of Activation of Second-Messenger-Generating Systems
Activation of Signal-Transducing Protein Kinases and Small GTPases / 9:
Akt Kinase Assay / 10:
vGPCR Stimulated Activation of Rac1-Pulldown Assays / 11:
Western Blotting Using Phospho-Specific Antibodies / 12:
Activation of Transcription Factors / 13:
Global Changes in Gene Expression: Microarray Analysis / 14:
NFkB Luciferase Assays / 15:
NFkB Binding Assays / 16:
Nuclear Translocation of NFkB / 17:
Pharmacological and Biochemical Characterization of Human Cytomegalovirus-Encoded G Protein-Coupled Receptors / David Maussang ; Henry F. Vischer ; Andreas Schreiber ; Detlef Michel ; Martine J. Smit
Virally Encoded GPCR Engineering
vGPCR Expression, Trafficking, and Radioligand Binding
vGPCR-Induced Signal Transduction
vGPCR-Induced Oncogenesis
Generation of Recombinant HCMV Strains by Markerless Bacterial Artificial Chromosome Mutagenesis
Conclusions
Identification and Characterization of Virus-Encoded Chemokine Binding Proteins / Antonio Alcami ; Abel Viejo-Borbolla
Methods for Studying Chemokine-Binding Proteins
The Chemokine-Binding Protein M3 as a Tool to Understand the Chemokine Network In Vivo / Sergio A. Lira ; Limin Shang ; Andrea P. Martin
Generation of Transgenic Mice Expressing M3 in Insulin-Producing b Cells
M3 Expression in Islets of Langerhans Blocks CCL2-, CCL21-, and CXCL13-Induced Migration of Cells to Islets
M3 Expression in b Cells Blocks Cellular Infiltration and Prevents Diabetes Development
Generation of a Conditional Transgenic System for Expression of M3
M-T7: Measuring Chemokine-Modulating Activity / Mee Y. Bartee ; Erbin Dai ; Liying Liu ; Ganesh Munuswamy-Ramanujam ; Colin Macaulay ; Dana Mclvor ; Grant McFadden ; Alexandra R. Lucas
Protein Expression
Quantifying the Effects of M-T7 In Vitro and Ex Vivo
Quantifying the Effects of M-T7 on Vascular Inflammatory Responses in Rodent Vascular Transplant Models
Preclinical Toxicity Testing
Atypical and Novel Chemoattractants and Receptors / Section III:
Role of the Chemokine Scavenger Receptor D6 in Balancing Inflammation and Immune Activation / Elena M. Borroni ; Chiara Buracchi ; Benedetta Savino ; Fabio Pasqualini ; Remo C. Russo ; Manuela Nebuloni ; Raffaella Bonecchi
Methods
Structure-Function Dissection of D6, an Atypical Scavenger Receptor / Robert J. B. Nibbs ; Pauline McLean ; Clare McCulloch ; Alan Riboldi-Tunnicliffe ; Emma Blair ; Yanshi Zhu ; Neil Isaacs ; Gerard J. Graham
Modeling Small Molecule-Compound Binding to G-Protein-Coupled Receptors / Nagarajan Vaidehi ; James E. Pease ; Richard Horuk
Similarity and Differences in the Crystal Structures of Class-A GPCRs Solved to Date
GPCR Modeling Methods
Computational Methods for Receptor Flexibility and Ligand-Induced Conformational Changes in GPCRs
Validation of GPCR-Ligand Models
Elucidation of Chemerin and Chemokine-Like Receptor-1 Function in Adipocytes by Adenoviral-Mediated shRNA Knockdown of Gene Expression / Kerry B. Goralski ; Christopher J. Sinal
The 3T3-L1 Cell Model for Adipogenesis and Adipocyte Metabolism
RNA Interference
Methods for Adenoviral shRNA Knockdown of Chemerin and CMKLR1 in 3T3-L1 Cells
Chemokine Signaling / Section IV:
Characterization of Chemokine Receptor CXCR2 Interacting Proteins Using a Proteomics Approach to Define the CXCR2 "Chemosynapse" / Dayanidhi Raman ; Nicole F. Neel ; Jiqing Sai ; Raymond L. Mernaugh ; Amy-Joan L. Ham ; Ann J. Richmond
Validation of the Interaction of Novel Proteins with CXCR2
Mutational Analysis of Residues at Interactive Interface of CXCR2 and CXCR2-Binding Proteins
Radioactive Phosphorylation of CXCR2 Interacting Proteins
Chemotaxis Assay
Phosphoproteomic Analysis of Chemokine Signaling Networks / Morgan O'Hayre ; Catherina L. Salanga ; Pieter C. Dorrestein ; Tracy M. Handel
Summary
Monitoring NF-kB Mediated Chemokine Transcription in Tumorigenesis / Jinming Yang
Development of NF-kB Reporter Model for Tumors
Bioluminescent Imaging of Intratumor Signaling of Anesthetized Mice
Cell-Based Assays for Kinase and Transcriptional Activity In Vitro
Peripheral Spying of Intratumoral Signaling of Conscious Mice
Analysis of Chemokine Receptor Endocytosis and Intracellular Trafficking / Tom Kershaw ; Silene T. Wavre-Shapton ; Nathalie Signoret ; Mark Marsh18:
Receptor Detection
Cells
Monitoring Receptor Endocytosis
Monitoring Receptor Recycling
Monitoring Receptor Degradation
Electron Microscopy Analysis of Receptor Internalization
Measuring the Proximity of T-Lymphocyte CXCR4 and TCR by Fluorescence Resonance Energy Transfer (FRET) / Ashok Kumar ; Kimberly N. Kremer ; Olivia L. Sims ; Karen E. Hedin19:
Assaying CXCR4-TCR Proximity via the PE/APC mAb FRET Approach
Assaying CXCR4-TCR Proximity via the CFP/YFP Fusion Protein Approach
Expression of CXCR4, a G-Protein-Coupled Receptor for CXCL12 in Yeast: Identification of New-Generation Inverse Agonists / Barry J. Evans ; Zixuan Wang ; James R. Broach ; Shinya Oishi ; Nobutaka Fujii ; Stephen C. Peiper20:
Methods and Discussion
Ubiquitination of Chemokine Receptors / Adriano Marchese21:
Cell Culture and Transfections
Agonist Treatment and Ubiquitination Assay
E3 Ubiquitin Ligase AIP4 Mediates Ubiquitination of CXCR4
Author Index
Subject Index
Contributors
Preface
Volumes in Series
3.

電子ブック

EB
Damon; Handel, Tracy; Hamel, Damon Hamel, Damon J. Hamel, Tracy M. Handel
出版情報: Elsevier ScienceDirect Books Complete , Academic Press, 2009
所蔵情報: loading…
目次情報: 続きを見る
Structure and Function
A Novel Chemokine-mediated In Vivo T Cell Recruitment Assay for Structure Function Studies / Andrew D. Luster ; Gabriele Campanella1:
Tyrosine sulfation of HIV-1 coreceptors and other chemokine-binding receptors / Micheal R. Farzan2:
Lymphotactin Structural Dynamics / Brian F. Volkman3:
Regulation of chemokines by posttranslational modifications / Paul Proost4:
Proteolytic Processing of Chemokines: Mass Spectrometry, Biochemistry, Neoepitope Antibody and Functional Characterization / Christopher Overall ; Amanda Starr5:
Multiple approaches to study Chemokine Receptor Homo and Heterodimerization / Mario Mellado6:
Heterodimerization of Chemokine Receptors with Other GPCRs / Graeme Milligan ; Shirley Appelbe7:
Homo and Hetero-Oligomerization of Chemokines / Tracy Handel ; Damon Hamel ; Ariane Jansma8:
Interactions of Chemokine with Glycosaminoglycans / Amanda Proudfoot9:
Structures of Chemokine:Chemokine Binding Protein Complexes / Andreas J. Kungl ; Daved Fremont10:
Activation Mechanisms of Chemokine Receptors / Mette Rosenkilde12:
Modeling Small Molecule Compound Binding to Receptors / Richard Horuk ; James Pease ; N. Vaidehi13:
Spectroscopic Approaches to Study Conformational Changes in GPCRs / David Farrens14:
Plasmon Resonance Methods in GPCR Signaling / Victor Hruby ; Isabel Alves ; Gordon Tollin15:
Flow Cytometric Analyses of GPCR Interactions or Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics / Larry Sklar16:
Expression and Functional Reconstitution of CCR1 / Samantha Allen17:
Characterization of the Interactions of Chemokines with Glycosaminoglycans by Fourier Transform Ion Cyclotron Resonance / Julie A. Leary18:
Chemokine Function in Cell Migration
In vitro assays for studying chemokine-triggered lymphocyte transendothelial migration under physiological flow conditions / Ronen Alon ; Francis Lin19:
Microfluidic device / Eugene Butcher20:
Two-photon imaging and quantitative analysis of leukocyte migration in vivo / Mark Miller ; Jenna Cash21:
Zymosan induced peritonitis as a model for studying chemokines and inflammatory mediators in vivo / David Greaves22:
Structure and Function
A Novel Chemokine-mediated In Vivo T Cell Recruitment Assay for Structure Function Studies / Andrew D. Luster ; Gabriele Campanella1:
Tyrosine sulfation of HIV-1 coreceptors and other chemokine-binding receptors / Micheal R. Farzan2:
4.

電子ブック

EB
Damon; Handel, Tracy; Hamel, Damon Hamel, Damon J. Hamel, Tracy M. Handel
出版情報: Elsevier ScienceDirect Books Complete , Academic Press, 2009
所蔵情報: loading…
目次情報: 続きを見る
Contributors
Preface
Volumes in Series
Chemokines and Receptors in Disease / Section I:
Chemokines in Human Breast Tumor Cells: Modifying Their Expression Levels and Determining Their Effects on the Malignancy Phenotype / Gali Soria ; Tsipi Meshel ; Adit Ben-Baruch1:
Introduction
Modifying Chemokine Expression in Breast Tumor Cells / 2:
Establishment of Primary Local Breast Tumors and Pulmonary Metastases / 3:
Acknowledgments
References
CCR5 Pharmacology Methodologies and Associated Applications / Roy Mansfield ; Sarah Able ; Paul Griffin ; Becky Irvine ; Ian James ; Malcolm Marcartney ; Ken Miller ; James Mills ; Carolyn Napier ; Iva Navratilova ; Manos Perros ; Graham Rickett ; Harriet Root ; Elna van der Ryst ; Mike Westby ; Patrick Dorr
CCR5 Signaling Assays and Application to Quantify and Characterize Ligand-Dependent Agonism, Antagonism, and Inverse Agonism
CCR5-Associated Ligand-Binding Assays
Surrogate In Vitro Antiviral Assays / 4:
CCR5 Site-Directed Mutagenesis and Ligand Docking Studies / 5:
Non-HIV Indications-Associated Studies, Human CCR5 Knock-In Mice / 6:
CXCR4 and Mobilization of Hematopoietic Precursors / Michael P. Rettig ; Pablo Ramirez ; Bruno Nervi ; John F. DiPersio
HSPC Mobilizing Agents that Target the CXCL12/CXCR4 Axis
Donor Selection for HSPC Mobilization
Flow Cytometric Enumeration of Mobilized HSPCs
Dosing and Kinetics of HSPC Mobilization by G-CSF and Plerixafor
Functional Characterization of Mobilized HSPCs / 7:
Concluding Remarks / 8:
Acknowledgment
Double-Label Nonradioactive In Situ Hybridization for the Analysis of Chemokine Receptor Expression in the Central Nervous System / Meizhang Li ; Richard M. Ransohoff
Basic Protocol for ISH (Using Digoxygenin-Labeled Probe)
Comments
Expression of Chemokines and Chemokine Receptors in Human Colon Cancer / Marco Erreni ; Paolo Bianchi ; Luigi Laghi ; Massimiliano Mirolo ; Marco Fabbri ; Massimo Locati ; Alberto Mantovani ; Paola Allavena
Materials and Methods
Results
Discussion
Chemokine Related Proteins from Pathogens / Section II:
Kaposi's Sarcoma Virally Encoded, G-Protein-Coupled Receptor: A Paradigm for Paracrine Transformation / Daniel Martin ; J. Silvio Gutkind
Cloning of vGPCR
Assaying vGPCR Transforming Activity In Vitro
vGPCR Transforming Activity In Vivo Using Xenograft Systems
In Vivo Targeted Infection Using the TVA-RCAS System
vGPCR-Induced Paracrine Transformation
Characterization of vGPCR-Induced Molecular Signaling
Evaluation of Activation of Second-Messenger-Generating Systems
Activation of Signal-Transducing Protein Kinases and Small GTPases / 9:
Akt Kinase Assay / 10:
vGPCR Stimulated Activation of Rac1-Pulldown Assays / 11:
Western Blotting Using Phospho-Specific Antibodies / 12:
Activation of Transcription Factors / 13:
Global Changes in Gene Expression: Microarray Analysis / 14:
NFkB Luciferase Assays / 15:
NFkB Binding Assays / 16:
Nuclear Translocation of NFkB / 17:
Pharmacological and Biochemical Characterization of Human Cytomegalovirus-Encoded G Protein-Coupled Receptors / David Maussang ; Henry F. Vischer ; Andreas Schreiber ; Detlef Michel ; Martine J. Smit
Virally Encoded GPCR Engineering
vGPCR Expression, Trafficking, and Radioligand Binding
vGPCR-Induced Signal Transduction
vGPCR-Induced Oncogenesis
Generation of Recombinant HCMV Strains by Markerless Bacterial Artificial Chromosome Mutagenesis
Conclusions
Identification and Characterization of Virus-Encoded Chemokine Binding Proteins / Antonio Alcami ; Abel Viejo-Borbolla
Methods for Studying Chemokine-Binding Proteins
The Chemokine-Binding Protein M3 as a Tool to Understand the Chemokine Network In Vivo / Sergio A. Lira ; Limin Shang ; Andrea P. Martin
Generation of Transgenic Mice Expressing M3 in Insulin-Producing b Cells
M3 Expression in Islets of Langerhans Blocks CCL2-, CCL21-, and CXCL13-Induced Migration of Cells to Islets
M3 Expression in b Cells Blocks Cellular Infiltration and Prevents Diabetes Development
Generation of a Conditional Transgenic System for Expression of M3
M-T7: Measuring Chemokine-Modulating Activity / Mee Y. Bartee ; Erbin Dai ; Liying Liu ; Ganesh Munuswamy-Ramanujam ; Colin Macaulay ; Dana Mclvor ; Grant McFadden ; Alexandra R. Lucas
Protein Expression
Quantifying the Effects of M-T7 In Vitro and Ex Vivo
Quantifying the Effects of M-T7 on Vascular Inflammatory Responses in Rodent Vascular Transplant Models
Preclinical Toxicity Testing
Atypical and Novel Chemoattractants and Receptors / Section III:
Role of the Chemokine Scavenger Receptor D6 in Balancing Inflammation and Immune Activation / Elena M. Borroni ; Chiara Buracchi ; Benedetta Savino ; Fabio Pasqualini ; Remo C. Russo ; Manuela Nebuloni ; Raffaella Bonecchi
Methods
Structure-Function Dissection of D6, an Atypical Scavenger Receptor / Robert J. B. Nibbs ; Pauline McLean ; Clare McCulloch ; Alan Riboldi-Tunnicliffe ; Emma Blair ; Yanshi Zhu ; Neil Isaacs ; Gerard J. Graham
Modeling Small Molecule-Compound Binding to G-Protein-Coupled Receptors / Nagarajan Vaidehi ; James E. Pease ; Richard Horuk
Similarity and Differences in the Crystal Structures of Class-A GPCRs Solved to Date
GPCR Modeling Methods
Computational Methods for Receptor Flexibility and Ligand-Induced Conformational Changes in GPCRs
Validation of GPCR-Ligand Models
Elucidation of Chemerin and Chemokine-Like Receptor-1 Function in Adipocytes by Adenoviral-Mediated shRNA Knockdown of Gene Expression / Kerry B. Goralski ; Christopher J. Sinal
The 3T3-L1 Cell Model for Adipogenesis and Adipocyte Metabolism
RNA Interference
Methods for Adenoviral shRNA Knockdown of Chemerin and CMKLR1 in 3T3-L1 Cells
Chemokine Signaling / Section IV:
Characterization of Chemokine Receptor CXCR2 Interacting Proteins Using a Proteomics Approach to Define the CXCR2 "Chemosynapse" / Dayanidhi Raman ; Nicole F. Neel ; Jiqing Sai ; Raymond L. Mernaugh ; Amy-Joan L. Ham ; Ann J. Richmond
Validation of the Interaction of Novel Proteins with CXCR2
Mutational Analysis of Residues at Interactive Interface of CXCR2 and CXCR2-Binding Proteins
Radioactive Phosphorylation of CXCR2 Interacting Proteins
Chemotaxis Assay
Phosphoproteomic Analysis of Chemokine Signaling Networks / Morgan O'Hayre ; Catherina L. Salanga ; Pieter C. Dorrestein ; Tracy M. Handel
Summary
Monitoring NF-kB Mediated Chemokine Transcription in Tumorigenesis / Jinming Yang
Development of NF-kB Reporter Model for Tumors
Bioluminescent Imaging of Intratumor Signaling of Anesthetized Mice
Cell-Based Assays for Kinase and Transcriptional Activity In Vitro
Peripheral Spying of Intratumoral Signaling of Conscious Mice
Analysis of Chemokine Receptor Endocytosis and Intracellular Trafficking / Tom Kershaw ; Silene T. Wavre-Shapton ; Nathalie Signoret ; Mark Marsh18:
Receptor Detection
Cells
Monitoring Receptor Endocytosis
Monitoring Receptor Recycling
Monitoring Receptor Degradation
Electron Microscopy Analysis of Receptor Internalization
Measuring the Proximity of T-Lymphocyte CXCR4 and TCR by Fluorescence Resonance Energy Transfer (FRET) / Ashok Kumar ; Kimberly N. Kremer ; Olivia L. Sims ; Karen E. Hedin19:
Assaying CXCR4-TCR Proximity via the PE/APC mAb FRET Approach
Assaying CXCR4-TCR Proximity via the CFP/YFP Fusion Protein Approach
Expression of CXCR4, a G-Protein-Coupled Receptor for CXCL12 in Yeast: Identification of New-Generation Inverse Agonists / Barry J. Evans ; Zixuan Wang ; James R. Broach ; Shinya Oishi ; Nobutaka Fujii ; Stephen C. Peiper20:
Methods and Discussion
Ubiquitination of Chemokine Receptors / Adriano Marchese21:
Cell Culture and Transfections
Agonist Treatment and Ubiquitination Assay
E3 Ubiquitin Ligase AIP4 Mediates Ubiquitination of CXCR4
Author Index
Subject Index
Contributors
Preface
Volumes in Series
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