| Why Purify Enzymes? / Murray Deutscher ; Arthur Kornberg |
| Developing Purification Procedures / Section I： |
| Strategies and Considerations for Protein Purifications / Stuart Linn |
| Use of Bioinformatics in Planning a Protein Purification / Richard R. Burgess |
| Preparing A Purification Summary Table |
| General Methods for Handling Proteins and Enzymes / Section II： |
| Setting Up A Laboratory / Murray P. Deutscher |
| Buffers: Principle and Practice / Vincent S. Stoll ; John S. Blanchard |
| Measurement of Enzyme Activity / T. K. Harris ; M. M. Keshwani |
| Quantitation of Protein / James E. Noble ; Marc J. A. Bailey |
| Concentration of Proteins and Removal of Solutes / Jonathan K. Romero ; David R. H. Evans ; Matthew Westoby |
| Maintaining Protein Stability |
| Recombinant Protein Expression and Purification / Section III： |
| Selecting an Appropriate Method for Expressing a Recombinant Protein / William H. Brondyk |
| Bacterial Systems for Production of Heterologous Proteins / Frank R. Collart ; Ashley M. Frank ; Sarah Zerbs |
| Expression in the Yeast Pichia Pastoris / James M. Cregg ; Ilya Tolstorukov ; Anasua Kusari ; Jay Sunga ; Knut Madden ; Thomas Chappell |
| Baculovirus-Insect Cell Expression Systems / Donald L. Jarvis |
| Recombinant Protein Production By Transient Gene Transfer Into Mammalian Cells / Sabine Geisse ; Cornelia Fux |
| Tagging For Protein Expression / Arun Malhotra |
| Refolding of Solubilized Inclusion Body Proteins |
| Preparation of Extracts and Subcellular Fractionation / Section IV： |
| Advances In Preparation of Biological Extracts for Protein Purification / Anthony C. Grabski |
| Isolation of Subcellular Organelles and Structures / Jörg Von Hagen ; Uwe Michelsen |
| Purification Procedures: Bulk Methods / Section V： |
| Protein Precipitation Techniques |
| Affi-Gel Blue for Nucleic Acid Removal and Early Enrichment of Nucleotide Binding Proteins |
| Purification Procedures: Chromatographic Methods / Section VI： |
| Ion Exchange Chromatography / Alois Jungbauer ; Rainer Hahn |
| Gel Filtration / Earle Stellwagen |
| Protein Chromatography on Hydroxyapatite Columns / Larry J. Cummings ; Mark A. Snyder ; Kimberly Brisack |
| Theory and Use of Hydrophobic Interaction Chromatography in Protein Purification Applications / Justin T. Mccue |
| Purification Procedures: Affininty Methods / Section VII： |
| Affinity Chromatography: General Methods / Marjeta Urh ; Dan Simpson ; Kate Zhao |
| Immobilized-Metal Affinity Chromatography (IMAC) / Frank Schäfer* Helena Block ; Barbara Maertens ; Anne Spriestersbach ; Nicole Brinker ; Jan Kubicek ; Roland Fabis And Jörg Labahn |
| Identification, Production, and Use of Polyol-Responsive Monoclonal Antibodies for Immunoaffinity Chromatography / Nancy E. Thompson ; Katherine M. Foley ; Elizabeth S. Stalder ; And Richard R. Burgess |
| Purification Procedures: Electrophoretic Methods / Section VIII： |
| One-Dimensional Gel Electrophoresis / David E. Garfin |
| Protein Analysis Using High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis and Isoelectric Focusing / Friedman |
| Protein Gel Staining Methods: An Introduction And Overview / Thomas H. Steinberg |
| Elution of Proteins From Gels |
| Immunodetection By Protein Blotting Performing and Optimizing Western Blots With An Emphasis on Chemiluminescent Detection / Alice Alegria-Schaffer ; Krishna Vattem ; Andrew Lodge |
| Purification Procedures: Membrane Proteins And Glycoproteins / Section IX： |
| Detergents: An Overview / Dirk Linke |
| Purification of Membrane Proteins / Sue-Hwa Lin ; Guido Guidotti |
| Purification of Recombinant G-Protein-Coupled Receptors / Reinhard Grisshammer |
| Cell-Free Translation Of Integral Membrane Proteins Into Unilamelar Liposomes / Michael A. Goren ; Akira Nozawa ; Shin-Ichi Makino ; Russell L. Wrobel ; Brian G. Fox |
| Characterization of Purified Proteins / Section X： |
| Determination of Protein Purity / David G. Rhodes ; Thomas M. Laue |
| Determination of Size, Molecular Weight, and Presence of Subunits / Robert E. Bossio |
| Identification and Quantification of Protein Posttranslational Modifications / Andrew Link |
| Parallel Methods For Expression And Purification / Scott A. Lesley |
| Techniques To Isolate O2-Sensitive Proteins: [4Fe-4S]-FNR As An Example / Aixin Yan ; Patricia J. Kiley |
| Rethinking Your Purification Procedure |
| Important But Little Known (Or Forgotten) Artifacts in Protein Biochemistry |
| Why Purify Enzymes? / Murray Deutscher ; Arthur Kornberg |
| Developing Purification Procedures / Section I： |
| Strategies and Considerations for Protein Purifications / Stuart Linn |
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