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1.

電子ブック

EB
David A. Hopwood, D. A. Hopwood, Professor of Genetics David A Hopwood
出版情報: Elsevier ScienceDirect Books , Academic Press, 2009
所蔵情報: loading…
目次情報: 続きを見る
Contributors
Preface
Volumes in Series
Polyketides / Section I:
Introduction to Polyketide Biosynthesis / Kira J. Weissman1:
Introduction
Acknowledgment
References
Structural Enzymology of Polyketide Synthases / Shiou-Chuan (Sheryl) Tsai ; Brian Douglas Ames2:
Fatty Acid Synthase
Different Types of PKS and a Summary of Structural Work / 3:
Structural Enzymology of Individual Domains / 4:
Summary and Future Prospects / 5:
Acknowledgments
Fungal Type I Polyketide Synthases / Russell J. Cox ; Thomas J. Simpson
Partially Reducing PKSs: 6-MethylSalicylate Synthase
Nonreducing PKSs
Highly Reducing PKSs
NR/HR PKS Hybrid Systems: Zearalenone (ZAE1 and ZAE2)
Conclusions / 6:
Tandem Acyl Carrier Protein Domains in Polyunsaturated Fatty Acid Synthases / Hui Jiang ; Scott R. Rajski ; Ben Shen
Methods
Conclusion
Iterative Type I Polyketide Synthases for Enediyne Core Biosynthesis / Geoffrey P. Horsman ; Steven G. Van Lanen
The DEBS Paradigm for Type I Modular Polyketide Synthases and Beyond / Leonard Katz
DEBS and the Concept of a Module
Beyond the DEBS Paradigm
Formation and Characterization of Acyl Carrier Protein-Linked Polyketide Synthase Extender Units / Yolande A. Chan ; Michael G. Thomas7:
Overproduction and Purification of Recombinant Proteins
Formation and Characterization of Hydroxymalonyl-ACP and Aminomalonyl-ACP
Type I Polyketide Synthases That Require Discrete Acyltransferases / Yi-Qiang Cheng ; Jane M. Coughlin ; Si-Kyu Lim8:
The Enzymology of Polyether Biosynthesis / Tiangang Liu ; David E. Cane ; Zixin Deng9:
Genetic Mining and Functional Analysis of Genes Specific to Polyether Biosynthetic Pathways
Premonensin, the Parent Unsaturated Monensin Polyketide
Epoxidases MonCl, NigCl, and NanO
Epoxide Hydrolases MonBI/BII, NigBI/BII, NanI, and Lsd19
NanE, a Polyether-Specific Thioesterase
Assay of Polyether Thioesterase Activity
Transcriptional Analysis of the Nanchangmycin Biosynthetic Pathway Genes
Enzymology of the Polyenes Pimaricin and Candicidin Biosynthesis / Juan F. Martin ; Jesus F. Aparicio10:
Pimaricin as a Prototype of Small Polyenes: Discovery and Properties
Pimaricin Biosynthesis in S. natalensis
Regulation of Pimaricin Biosynthesis
Candicidin: A Prototype of "Aromatic" Polyenes
The Candicidin/FR-008 Gene Cluster
Biosynthesis of PABA: The pabAB and pabC Genes
The Polyketide Synthases
Monooxygenase Genes: Modifications of the Polyketide Chain
Transporter Genes
Genes Related to Mycosamine Biosynthesis / 11:
Regulatory Genes / 12:
Phosphate Represses Expression of the pabAB Gene / 13:
Future Perspectives / 14:
Genetic Analysis of Nystatin and Amphotericin Biosynthesis / Sergey Zotchev ; Patrick Caffrey
Gene Inactivation and Replacement in the Nystatin Producer Streptomyces noursei
Gene Inactivation and Replacement in the Amphotericin Producer Streptomyces nodosus
Production, Purification, and Characterization of Novel Amphotericin- and Nystatin-Related Polyenes
Polyketide Versatility in the Biosynthesis of Complex Mycobacterial Cell Wall Lipids / Tarun Chopra ; Rajesh S. Gokhale
Acetate and Propionate Feeding Studies
Genome Sequencing and Identification of Polyketide Synthases
Mycobacterial Polyketide Synthases
Techniques to Characterize PKS Enzymes
Genetic Engineering to Produce Polyketide Analogues / Christopher D. Reeves ; Eduardo Rodriguez
AT Domain Replacement to Alter a-Carbon Substitution
Procedure for Engineering AT Replacements in the Chromosome
Engineering b-Carbon Processing
Engineering when only a Single Crossover Event is Possible
Heterologous Expression of Engineered PKS Genes
Chemobiosynthesis
Mutasynthesis
Gene Knockouts to Obtain Analogues
Design and Synthesis of Pathway Genes for Polyketide Biosynthesis / Salvador Peiru ; Hugo Gramajo ; Hugo G. Menzella
Redesign of PKS Genes to Accommodate Unique Restriction Sites Flanking Individual Components and for Efficient Expression in E. coli
Validation of Synthetic PKS Gene Design
A Rapid Assay to Identify Productive Combinations of PKS Modules
Assembly of Larger Polyketide Synthases Using Information Gained with the Bimodular Assay
Design and Construction of Synthetic Operons for the Expression of Sugar Pathway Genes
Heterologous Production of Polyketides in Bacteria / 15:
General Considerations for the Heterologous Expression of Polyketide Pathways
S. coelicolor as a Model System for Heterologous Expression of Polyketides
Procedure for the Heterologous Production of Polyketides in S. coelicolor
System Improvements for the Heterologous Production of Polyketides in Streptomyces spp
Recent Developments for the Production of Polyketides in Nonactinomycete Bacteria
In Vitro Analysis of Type II Polyketide Synthase / Wenjun Zhang ; Yi Tang16:
Expression and Purification of Type II PKSs
In Vitro Activity Assays
Bacterial Fatty Acid Synthesis and its Relationships with Polyketide Synthetic Pathways / John E. Cronan ; Jacob Thomas17:
Bacterial Fatty Acids
Acyl Carrier Protein, the Key Component of Bacterial Fatty Acid Synthesis
Overview of the Reactions of Fatty Acid Biosynthesis
The Initiation Steps of Fatty Acid Synthesis
The Enzymes of the Fatty Acid Elongation Cycle
Unsaturated Fatty Acid Synthesis in E. coli
The Abundant Exceptions to the E. coli Fatty Acid Synthesis Paradigm
Relationships between Fatty Acid Synthesis and Polyketide Synthesis
Methods for Study of Bacterial Fatty Acid Synthesis
Aminocoumarins / Section II:
Aminocoumarins: Mutasynthesis, Chemoenzymatic Synthesis, and Metabolic Engineering / Lutz Heide18:
Generation of Integrative Cosmids and Heterologous Expression of Novobiocin, Clorobiocin, and Coumermycin A1 Gene Clusters
Generation of Single and or Multiple Deletions in the Integrative Cosmids
Mutasynthetic Generation of New Aminocoumarin Antibiotics
In Vitro Amide Synthetase Assays for the Identification of Suitable Ring A Analogues for Mutasynthesis
Use of Various Amide Synthetase Genes for Expanding Mutasynthesis Product Range
Generation of Substrates for Chemoenzymatic Synthesis
Chemoenzymatic Synthesis of New Clorobiocin Analogues
Generation of New Aminocoumarin Antibiotics by Metabolic Engineering
Carbohydrates / Section III:
Enzymology of Aminoglycoside Biosynthesis-Deduction from Gene Clusters / Udo F. Wehmeier ; Wolfgang Piepersberg19:
Some Key Enzymes in the Biosyntheses of Aminoglycoside Antibiotics
Acarbose and Related Metabolites
Biosynthetic Enzymes for the Aminoglycosides Butirosin and Neomycin / Fumitaka Kudo ; Tadashi Eguchi20:
General Method to Investigate Functions of the Biosynthetic Enzymes for Aminoglycosides
Neamine Biosynthetic Enzymes (Enzymes 1
Contributors
Preface
Volumes in Series
2.

電子ブック

EB
David A. Hopwood, D. A. Hopwood, Professor of Genetics David A Hopwood
出版情報: Elsevier ScienceDirect Books , Academic Press, 2009
所蔵情報: loading…
目次情報: 続きを見る
Overview articles / Part 1:
Introduction - chemical classes of complex natural products and overview of their biosynthesis
Discovery of novel natural products
Trans-modification of intermediates on the assembly line in PKS, FAS and NRPS biosynthesis
Post-assembly line modifications, including glycosylation
Cloning and analysis of natural product pathways
In silico prediction of pathways from DNA sequence
Mass spectrometric analysis of PKS and NRPS biosynthetic intermediates
Synthetic probes for PKS and NRPS biochemistry
Directed evolution of natural products
Expression in a heterologous host
Pathway-specific and global regulation of antibiotic biosynthesis
Regulation of antibiotic production by bacterial hormones
Peptides / Part 2:
Non-ribosomally-synthesised peptides Overview of NRPSs, and mechanistic and structural studies / A:
Siderophores Cephamycins and clavulanic acid
Glycopeptides
Lipopeptides
Precursor
Biosynthesis
Ribosomally synthesised peptides / B:
Lantibiotic biosynthesis and engineering of novel compounds
Cyclic peptides from marine bacteria
Overview articles / Part 1:
Introduction - chemical classes of complex natural products and overview of their biosynthesis
Discovery of novel natural products
3.

電子ブック

EB
David A. Hopwood, D. A. Hopwood, Professor of Genetics David A Hopwood
出版情報: Elsevier ScienceDirect Books Complete , Academic Press, 2009
所蔵情報: loading…
目次情報: 続きを見る
Overview articles / Part 1:
Introduction - chemical classes of complex natural products and overview of their biosynthesis
Discovery of novel natural products
Trans-modification of intermediates on the assembly line in PKS, FAS and NRPS biosynthesis
Post-assembly line modifications, including glycosylation
Cloning and analysis of natural product pathways
In silico prediction of pathways from DNA sequence
Mass spectrometric analysis of PKS and NRPS biosynthetic intermediates
Synthetic probes for PKS and NRPS biochemistry
Directed evolution of natural products
Expression in a heterologous host
Pathway-specific and global regulation of antibiotic biosynthesis
Regulation of antibiotic production by bacterial hormones
Peptides / Part 2:
Non-ribosomally-synthesised peptides Overview of NRPSs, and mechanistic and structural studies / A:
Siderophores Cephamycins and clavulanic acid
Glycopeptides
Lipopeptides
Precursor
Biosynthesis
Ribosomally synthesised peptides / B:
Lantibiotic biosynthesis and engineering of novel compounds
Cyclic peptides from marine bacteria
Overview articles / Part 1:
Introduction - chemical classes of complex natural products and overview of their biosynthesis
Discovery of novel natural products
4.

電子ブック

EB
David A. Hopwood, D. A. Hopwood, Professor of Genetics David A Hopwood
出版情報: Elsevier ScienceDirect Books Complete , Academic Press, 2009
所蔵情報: loading…
目次情報: 続きを見る
Contributors
Preface
Volumes in Series
Polyketides / Section I:
Introduction to Polyketide Biosynthesis / Kira J. Weissman1:
Introduction
Acknowledgment
References
Structural Enzymology of Polyketide Synthases / Shiou-Chuan (Sheryl) Tsai ; Brian Douglas Ames2:
Fatty Acid Synthase
Different Types of PKS and a Summary of Structural Work / 3:
Structural Enzymology of Individual Domains / 4:
Summary and Future Prospects / 5:
Acknowledgments
Fungal Type I Polyketide Synthases / Russell J. Cox ; Thomas J. Simpson
Partially Reducing PKSs: 6-MethylSalicylate Synthase
Nonreducing PKSs
Highly Reducing PKSs
NR/HR PKS Hybrid Systems: Zearalenone (ZAE1 and ZAE2)
Conclusions / 6:
Tandem Acyl Carrier Protein Domains in Polyunsaturated Fatty Acid Synthases / Hui Jiang ; Scott R. Rajski ; Ben Shen
Methods
Conclusion
Iterative Type I Polyketide Synthases for Enediyne Core Biosynthesis / Geoffrey P. Horsman ; Steven G. Van Lanen
The DEBS Paradigm for Type I Modular Polyketide Synthases and Beyond / Leonard Katz
DEBS and the Concept of a Module
Beyond the DEBS Paradigm
Formation and Characterization of Acyl Carrier Protein-Linked Polyketide Synthase Extender Units / Yolande A. Chan ; Michael G. Thomas7:
Overproduction and Purification of Recombinant Proteins
Formation and Characterization of Hydroxymalonyl-ACP and Aminomalonyl-ACP
Type I Polyketide Synthases That Require Discrete Acyltransferases / Yi-Qiang Cheng ; Jane M. Coughlin ; Si-Kyu Lim8:
The Enzymology of Polyether Biosynthesis / Tiangang Liu ; David E. Cane ; Zixin Deng9:
Genetic Mining and Functional Analysis of Genes Specific to Polyether Biosynthetic Pathways
Premonensin, the Parent Unsaturated Monensin Polyketide
Epoxidases MonCl, NigCl, and NanO
Epoxide Hydrolases MonBI/BII, NigBI/BII, NanI, and Lsd19
NanE, a Polyether-Specific Thioesterase
Assay of Polyether Thioesterase Activity
Transcriptional Analysis of the Nanchangmycin Biosynthetic Pathway Genes
Enzymology of the Polyenes Pimaricin and Candicidin Biosynthesis / Juan F. Martin ; Jesus F. Aparicio10:
Pimaricin as a Prototype of Small Polyenes: Discovery and Properties
Pimaricin Biosynthesis in S. natalensis
Regulation of Pimaricin Biosynthesis
Candicidin: A Prototype of "Aromatic" Polyenes
The Candicidin/FR-008 Gene Cluster
Biosynthesis of PABA: The pabAB and pabC Genes
The Polyketide Synthases
Monooxygenase Genes: Modifications of the Polyketide Chain
Transporter Genes
Genes Related to Mycosamine Biosynthesis / 11:
Regulatory Genes / 12:
Phosphate Represses Expression of the pabAB Gene / 13:
Future Perspectives / 14:
Genetic Analysis of Nystatin and Amphotericin Biosynthesis / Sergey Zotchev ; Patrick Caffrey
Gene Inactivation and Replacement in the Nystatin Producer Streptomyces noursei
Gene Inactivation and Replacement in the Amphotericin Producer Streptomyces nodosus
Production, Purification, and Characterization of Novel Amphotericin- and Nystatin-Related Polyenes
Polyketide Versatility in the Biosynthesis of Complex Mycobacterial Cell Wall Lipids / Tarun Chopra ; Rajesh S. Gokhale
Acetate and Propionate Feeding Studies
Genome Sequencing and Identification of Polyketide Synthases
Mycobacterial Polyketide Synthases
Techniques to Characterize PKS Enzymes
Genetic Engineering to Produce Polyketide Analogues / Christopher D. Reeves ; Eduardo Rodriguez
AT Domain Replacement to Alter a-Carbon Substitution
Procedure for Engineering AT Replacements in the Chromosome
Engineering b-Carbon Processing
Engineering when only a Single Crossover Event is Possible
Heterologous Expression of Engineered PKS Genes
Chemobiosynthesis
Mutasynthesis
Gene Knockouts to Obtain Analogues
Design and Synthesis of Pathway Genes for Polyketide Biosynthesis / Salvador Peiru ; Hugo Gramajo ; Hugo G. Menzella
Redesign of PKS Genes to Accommodate Unique Restriction Sites Flanking Individual Components and for Efficient Expression in E. coli
Validation of Synthetic PKS Gene Design
A Rapid Assay to Identify Productive Combinations of PKS Modules
Assembly of Larger Polyketide Synthases Using Information Gained with the Bimodular Assay
Design and Construction of Synthetic Operons for the Expression of Sugar Pathway Genes
Heterologous Production of Polyketides in Bacteria / 15:
General Considerations for the Heterologous Expression of Polyketide Pathways
S. coelicolor as a Model System for Heterologous Expression of Polyketides
Procedure for the Heterologous Production of Polyketides in S. coelicolor
System Improvements for the Heterologous Production of Polyketides in Streptomyces spp
Recent Developments for the Production of Polyketides in Nonactinomycete Bacteria
In Vitro Analysis of Type II Polyketide Synthase / Wenjun Zhang ; Yi Tang16:
Expression and Purification of Type II PKSs
In Vitro Activity Assays
Bacterial Fatty Acid Synthesis and its Relationships with Polyketide Synthetic Pathways / John E. Cronan ; Jacob Thomas17:
Bacterial Fatty Acids
Acyl Carrier Protein, the Key Component of Bacterial Fatty Acid Synthesis
Overview of the Reactions of Fatty Acid Biosynthesis
The Initiation Steps of Fatty Acid Synthesis
The Enzymes of the Fatty Acid Elongation Cycle
Unsaturated Fatty Acid Synthesis in E. coli
The Abundant Exceptions to the E. coli Fatty Acid Synthesis Paradigm
Relationships between Fatty Acid Synthesis and Polyketide Synthesis
Methods for Study of Bacterial Fatty Acid Synthesis
Aminocoumarins / Section II:
Aminocoumarins: Mutasynthesis, Chemoenzymatic Synthesis, and Metabolic Engineering / Lutz Heide18:
Generation of Integrative Cosmids and Heterologous Expression of Novobiocin, Clorobiocin, and Coumermycin A1 Gene Clusters
Generation of Single and or Multiple Deletions in the Integrative Cosmids
Mutasynthetic Generation of New Aminocoumarin Antibiotics
In Vitro Amide Synthetase Assays for the Identification of Suitable Ring A Analogues for Mutasynthesis
Use of Various Amide Synthetase Genes for Expanding Mutasynthesis Product Range
Generation of Substrates for Chemoenzymatic Synthesis
Chemoenzymatic Synthesis of New Clorobiocin Analogues
Generation of New Aminocoumarin Antibiotics by Metabolic Engineering
Carbohydrates / Section III:
Enzymology of Aminoglycoside Biosynthesis-Deduction from Gene Clusters / Udo F. Wehmeier ; Wolfgang Piepersberg19:
Some Key Enzymes in the Biosyntheses of Aminoglycoside Antibiotics
Acarbose and Related Metabolites
Biosynthetic Enzymes for the Aminoglycosides Butirosin and Neomycin / Fumitaka Kudo ; Tadashi Eguchi20:
General Method to Investigate Functions of the Biosynthetic Enzymes for Aminoglycosides
Neamine Biosynthetic Enzymes (Enzymes 1
Contributors
Preface
Volumes in Series
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