Contributors |
Preface |
Volumes in Series |
Polyketides / Section I: |
Introduction to Polyketide Biosynthesis / Kira J. Weissman1: |
Introduction |
Acknowledgment |
References |
Structural Enzymology of Polyketide Synthases / Shiou-Chuan (Sheryl) Tsai ; Brian Douglas Ames2: |
Fatty Acid Synthase |
Different Types of PKS and a Summary of Structural Work / 3: |
Structural Enzymology of Individual Domains / 4: |
Summary and Future Prospects / 5: |
Acknowledgments |
Fungal Type I Polyketide Synthases / Russell J. Cox ; Thomas J. Simpson |
Partially Reducing PKSs: 6-MethylSalicylate Synthase |
Nonreducing PKSs |
Highly Reducing PKSs |
NR/HR PKS Hybrid Systems: Zearalenone (ZAE1 and ZAE2) |
Conclusions / 6: |
Tandem Acyl Carrier Protein Domains in Polyunsaturated Fatty Acid Synthases / Hui Jiang ; Scott R. Rajski ; Ben Shen |
Methods |
Conclusion |
Iterative Type I Polyketide Synthases for Enediyne Core Biosynthesis / Geoffrey P. Horsman ; Steven G. Van Lanen |
The DEBS Paradigm for Type I Modular Polyketide Synthases and Beyond / Leonard Katz |
DEBS and the Concept of a Module |
Beyond the DEBS Paradigm |
Formation and Characterization of Acyl Carrier Protein-Linked Polyketide Synthase Extender Units / Yolande A. Chan ; Michael G. Thomas7: |
Overproduction and Purification of Recombinant Proteins |
Formation and Characterization of Hydroxymalonyl-ACP and Aminomalonyl-ACP |
Type I Polyketide Synthases That Require Discrete Acyltransferases / Yi-Qiang Cheng ; Jane M. Coughlin ; Si-Kyu Lim8: |
The Enzymology of Polyether Biosynthesis / Tiangang Liu ; David E. Cane ; Zixin Deng9: |
Genetic Mining and Functional Analysis of Genes Specific to Polyether Biosynthetic Pathways |
Premonensin, the Parent Unsaturated Monensin Polyketide |
Epoxidases MonCl, NigCl, and NanO |
Epoxide Hydrolases MonBI/BII, NigBI/BII, NanI, and Lsd19 |
NanE, a Polyether-Specific Thioesterase |
Assay of Polyether Thioesterase Activity |
Transcriptional Analysis of the Nanchangmycin Biosynthetic Pathway Genes |
Enzymology of the Polyenes Pimaricin and Candicidin Biosynthesis / Juan F. Martin ; Jesus F. Aparicio10: |
Pimaricin as a Prototype of Small Polyenes: Discovery and Properties |
Pimaricin Biosynthesis in S. natalensis |
Regulation of Pimaricin Biosynthesis |
Candicidin: A Prototype of "Aromatic" Polyenes |
The Candicidin/FR-008 Gene Cluster |
Biosynthesis of PABA: The pabAB and pabC Genes |
The Polyketide Synthases |
Monooxygenase Genes: Modifications of the Polyketide Chain |
Transporter Genes |
Genes Related to Mycosamine Biosynthesis / 11: |
Regulatory Genes / 12: |
Phosphate Represses Expression of the pabAB Gene / 13: |
Future Perspectives / 14: |
Genetic Analysis of Nystatin and Amphotericin Biosynthesis / Sergey Zotchev ; Patrick Caffrey |
Gene Inactivation and Replacement in the Nystatin Producer Streptomyces noursei |
Gene Inactivation and Replacement in the Amphotericin Producer Streptomyces nodosus |
Production, Purification, and Characterization of Novel Amphotericin- and Nystatin-Related Polyenes |
Polyketide Versatility in the Biosynthesis of Complex Mycobacterial Cell Wall Lipids / Tarun Chopra ; Rajesh S. Gokhale |
Acetate and Propionate Feeding Studies |
Genome Sequencing and Identification of Polyketide Synthases |
Mycobacterial Polyketide Synthases |
Techniques to Characterize PKS Enzymes |
Genetic Engineering to Produce Polyketide Analogues / Christopher D. Reeves ; Eduardo Rodriguez |
AT Domain Replacement to Alter a-Carbon Substitution |
Procedure for Engineering AT Replacements in the Chromosome |
Engineering b-Carbon Processing |
Engineering when only a Single Crossover Event is Possible |
Heterologous Expression of Engineered PKS Genes |
Chemobiosynthesis |
Mutasynthesis |
Gene Knockouts to Obtain Analogues |
Design and Synthesis of Pathway Genes for Polyketide Biosynthesis / Salvador Peiru ; Hugo Gramajo ; Hugo G. Menzella |
Redesign of PKS Genes to Accommodate Unique Restriction Sites Flanking Individual Components and for Efficient Expression in E. coli |
Validation of Synthetic PKS Gene Design |
A Rapid Assay to Identify Productive Combinations of PKS Modules |
Assembly of Larger Polyketide Synthases Using Information Gained with the Bimodular Assay |
Design and Construction of Synthetic Operons for the Expression of Sugar Pathway Genes |
Heterologous Production of Polyketides in Bacteria / 15: |
General Considerations for the Heterologous Expression of Polyketide Pathways |
S. coelicolor as a Model System for Heterologous Expression of Polyketides |
Procedure for the Heterologous Production of Polyketides in S. coelicolor |
System Improvements for the Heterologous Production of Polyketides in Streptomyces spp |
Recent Developments for the Production of Polyketides in Nonactinomycete Bacteria |
In Vitro Analysis of Type II Polyketide Synthase / Wenjun Zhang ; Yi Tang16: |
Expression and Purification of Type II PKSs |
In Vitro Activity Assays |
Bacterial Fatty Acid Synthesis and its Relationships with Polyketide Synthetic Pathways / John E. Cronan ; Jacob Thomas17: |
Bacterial Fatty Acids |
Acyl Carrier Protein, the Key Component of Bacterial Fatty Acid Synthesis |
Overview of the Reactions of Fatty Acid Biosynthesis |
The Initiation Steps of Fatty Acid Synthesis |
The Enzymes of the Fatty Acid Elongation Cycle |
Unsaturated Fatty Acid Synthesis in E. coli |
The Abundant Exceptions to the E. coli Fatty Acid Synthesis Paradigm |
Relationships between Fatty Acid Synthesis and Polyketide Synthesis |
Methods for Study of Bacterial Fatty Acid Synthesis |
Aminocoumarins / Section II: |
Aminocoumarins: Mutasynthesis, Chemoenzymatic Synthesis, and Metabolic Engineering / Lutz Heide18: |
Generation of Integrative Cosmids and Heterologous Expression of Novobiocin, Clorobiocin, and Coumermycin A1 Gene Clusters |
Generation of Single and or Multiple Deletions in the Integrative Cosmids |
Mutasynthetic Generation of New Aminocoumarin Antibiotics |
In Vitro Amide Synthetase Assays for the Identification of Suitable Ring A Analogues for Mutasynthesis |
Use of Various Amide Synthetase Genes for Expanding Mutasynthesis Product Range |
Generation of Substrates for Chemoenzymatic Synthesis |
Chemoenzymatic Synthesis of New Clorobiocin Analogues |
Generation of New Aminocoumarin Antibiotics by Metabolic Engineering |
Carbohydrates / Section III: |
Enzymology of Aminoglycoside Biosynthesis-Deduction from Gene Clusters / Udo F. Wehmeier ; Wolfgang Piepersberg19: |
Some Key Enzymes in the Biosyntheses of Aminoglycoside Antibiotics |
Acarbose and Related Metabolites |
Biosynthetic Enzymes for the Aminoglycosides Butirosin and Neomycin / Fumitaka Kudo ; Tadashi Eguchi20: |
General Method to Investigate Functions of the Biosynthetic Enzymes for Aminoglycosides |
Neamine Biosynthetic Enzymes (Enzymes 1 |