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1.

電子ブック
ＥＢ
1. Autophagy in Mammalian Systems
Daniel; Klionsky, Daniel Klionsky, Daniel J. Klionsky
出版情報: Elsevier ScienceDirect Books , Academic Press, 2009
所蔵情報: loading…
目次情報: 続きを見る
LC3-based assay for monitoring autophagy / Shunsuke Kimura ; Naonobu Fujita ; Takeshi Noda ; Tamotsu YoshimoriChapter 1：
Analysis of autophagy using GFP-LC3 transgenic mice / Noboru MizushimaChapter 2：
Photoactivatable GFP / Jennifer Lippincott-Schwartz ; Dale HaileyChapter 3：
Assaying for autophagic protein degradation / Fred MeijerChapter 4：
Sequestration assays for mammalian autophagy / Per O. SeglenChapter 5：
Incorporation of monodansylcadaverine (MDC) as an assay to assess autophagy induction and monitoring fusion with a degradative compartment / Maria Isabel ColomboChapter 6：
The GST-BHMT assay and related assays for autophagy / Carol A. Mercer ; Patrick B. DennisChapter 7：
Redox and autophagy-ROS as an indicator for autophagic activity / Ruth Scherz-Shouval ; Zvulun ElazarChapter 8：
FACS analysis of autophagy / Elena Shvets ; Ephraim FassChapter 9：
Electron microscopy in mammals / PSivi YlS-Anttila ; Eeva-Liisa EskelinenChapter 10：
Monitoring mTOR activity / Ken InokiChapter 11：
Using p62 as a marker for autophagy / Terje JohansenChapter 12：
Cytosolic LC3 ratio as a quantitative index of macroautophagy / Motoni Kadowaki ; Md. Razaul KarimChapter 13：
Pexophagy in mammalian cells / Junji Ezaki ; Takashi Ueno ; Eiki Kominami ; Masaaki KomatsuChapter 14：
Mitophagy in mammalian cells / Ji Zhang ; Paul A. NeyChapter 15：
Quantification of WIPI-1/Atg18 puncta formation for assessing mammalian autophagy / Tassula Proikas-Cezanne ; Simon G. PfistererChapter 16：
Correlative light and electron microscopy (CLEM) using GFP-LC3 and other autophagosome markers / Sharon Tooze ; Minoo RaziChapter 17：
Nanotechnology assays for autophagy / Oleksandr SeleverstovChapter 18：
Photoconvertible KAEDA-LC3 for monitoring autophagic flux / Christoph Goemans ; Aviva TolkovskyChapter 19：
Methods to monitor Chaperone-mediated autophagy / Ana Maria Cuervo ; Susmita KaushikChapter 20：
Autophagy in response to Salmonella enterica serovar Typhimurium infection / Cheryl Birmingham ; John BrumellChapter 21：
Monitoring autophagy during Mycobacterium tuberculosis infection / Vojo DereticChapter 22：
Shigella, Listeria and Streptococcus induction of autophagy / Michinaga Ogawa ; Yuko Yoshikawa ; Ichiro Nakagawa ; Chihiro Sasakawa ; Trinad ChakrabortyChapter 23：
Measuring the impact of infection on autophagy in macrophages / Jean-Francois Dubuisson ; Brenda Byrne ; Michele SwansonChapter 24：
LC3-based assay for monitoring autophagy / Shunsuke Kimura ; Naonobu Fujita ; Takeshi Noda ; Tamotsu YoshimoriChapter 1：
Analysis of autophagy using GFP-LC3 transgenic mice / Noboru MizushimaChapter 2：
Photoactivatable GFP / Jennifer Lippincott-Schwartz ; Dale HaileyChapter 3：
2.

電子ブック
ＥＢ
2. Autophagy in Disease and Clinical Applications
Daniel; Klionsky, Daniel Klionsky, Daniel J. Klionsky
出版情報: Elsevier ScienceDirect Books , Academic Press, 2009
所蔵情報: loading…
目次情報: 続きを見る
Contributors
Preface
Volumes in Series
Initiation of Autophagy by Photodynamic Therapy / David Kessel ; Nancy L. Oleinick1：
Introduction
Photosensitizing Agents / 2：
Additional Factors Unique to PDT / 3：
A Typical PDT Protocol / 4：
Identification and Characterization of Autophagy after PDT / 5：
Effects of Autophagy on PDT Responses / 6：
Acknowledgments
References
Autophagic Cell Death / Michael J. Lenardo ; Christina K. McPhee ; Li Yu
Methods to Quantify Cell Death
Methods to Measure Autophagy
Methods to Establish Autophagy as the Cause of Cell Death
Autophagy Genes for RNAi Silencing
Transfact RNAi by Electroporation (Amaxa Nucleofection)
Conclusion / 7：
Autophagic Neuron Death / Yasuo Uchiyama ; Masato Koike ; Masahiro Shibata ; Mitsuho Sasaki
Experimental Models of Neurodegeneration
Measurements of Neuron Death
Assessing Metabolic Stress and Autophagy Status in Epithelial Tumors / Robin Mathew ; Vassiliki Karantza-Wadsworth ; Eileen White
Mouse Epithelial Cell Models for Studying the Role of Autophagy in Cancer
Protocols for Monitoring Autophagy in iBMK Cells and iMMECs In Vitro
Protocols for Monitoring Autophagy in Tumors In Vivo
Monitoring Chromosomal Instability Due to Autophagy Defects
Concluding Remarks and Future Perspectives
Autophagic Clearance of Aggregate-Prone Proteins Associated with Neurodegeneration / Sovan Sarkar ; Brinda Ravikumar ; David C. Rubinsztein
Aggregate-Prone Intracytoplasmic Proteins Associated with Neurodegenerative Disorders are Autophagy Substrates
Assays for the Clearance of Aggregate-Prone Proteins
Measurement of Autophagic Flux Using Bafilomycin A1
Concluding Remarks
Acknowledgements
Monitoring Autophagy in Alzheimer's Disease and Related Neurodegenerative Diseases / Dun-Sheng Yang ; Ju-Hyun Lee ; Ralph A. Nixon
General Approaches to Investigations of Human Neurodegeneration
Characterization of Autophagic Vacuoles, Evaluation of Autophagosome and Autolysosome Formation, and Autolysosomal Clearance
Metabolic Analyses of Autophagy in Neuronal and Nonneuronal Cell Models
Isolation and Characterization of Autophagic Vacuoles and Lysosomes from Cell Cultures and Brain Tissue
Western Blot Analysis of Autophagy Components and Substrates
Live-Cell Imaging of Autophagy Induction and Autophagosome-Lysosome Fusion in Primary Cultured Neurons / Mona Bains ; Kim A. Heidenreich
Cultured Cerebellar Purkinje Neurons as a Model to Study Neuronal Autophagy
Characterization of Autophagic Vacuole Size and Number in Purkinje Neurons
Using Colocalization of Fluorescent Tags to Measure Autophagosome-Lysosome Fusion
Using Genetic Mouse Models to Study the Biology and Pathology of Autophagy in the Central Nervous System / Zhenyu Yue ; Gay R. Holstein ; Brian T. Chait ; Qing Jun Wang8：
Methods
Analysis of GFP-LC3 Expression and Subcellular Localization in the CNS
Analysis of p62/SQSTM1 and Ubiquitinated Protein Inclusions in the CNS
Transmission Electron Microscopy (TEM) Analysis of Autophagosomes
Biochemical and Morphological Detection of Inclusion Bodies in Autophagy-Deficient Mice / Satoshi Waguri ; Masaaki Komatsu9：
Detection of Ubiquitinated Proteins and p62 in Autophagy-Deficient Mice by Western Blot Analysis
Detection of Ubiquitinated Proteins and p62 in Cultured Hepatocytes Derived from Autophagy-Deficient Mice
Detection of Ubiquitin-and p62-Positive Inclusions at the Light Microscopy Level
Detection of Ubiquitin-and p62-Positive Inclusions at the Electron Microscopy Level
Analyzing Autophagy in Clinical Tissues of Lung and Vascular Diseases / Hong Pyo Kim ; Zhi-Hua Chen ; Augustine M. K. Choi ; Stefan W. Ryter10：
Methods for Preparation of Lung and Vascular Cells
Analysis of Autophagy
Chromatin Immunoprecipitation
Conclusions
Autophagy in Neurite Injury and Neurodegeneration: In Vitro and In Vivo Models / Charleen T. Chu ; Edward D. Plowey ; Ruben K. Dagda ; Robert W. Hickey ; Salvatore J. Cherra ; Robert S. B. Clark11：
Studying Neuronal Autophagy In Vitro
Studying Brain Autophagy In Vivo
Future Perspectives and Challenges
Monitoring the Autophagy Pathway in Cancer / Frank C. Dorsey ; Meredith A. Steeves ; Stephanie M. Prater ; Thomas Schröter ; John L. Cleveland12：
LC3: A Phenotypic and Functional Marker of Autophagy
Assessing the Role of Autophagy in E&umu;-Myc-Driven Lymphoma
Autophagy Pathways in Glioblastoma / Hong Jiang ; Erin J. White ; Charles Conrad ; Candelaria Gomez-Manzano ; Juan Fueyo13：
Introduction: Autophagy and Gliomas
Prioritization of Methods to Characterize Autophagy in Gliomas
In Vitro Cellular Markers
In Vitro Biochemical Markers
Electron Microscopy to Monitor the Autophagic Vacuoles
In Vivo Analysis of Biochemical Markers
Autophagy Indicators as Surrogate Markets of Treatment Effect in Clinical Trials
Future Directions
Autophagy in Lung Cancer / Jerry J. Jaboin ; Misun Hwang ; Bo Lu14：
Signal-Dependant Control of Autophagy-Related Gene Expression / Fulvio Chiacchiera ; Cristiano Simone15：
Overview: Signal Transduction and Chromatin-Associated Kinases
The p38 Pathway in Colorectal Cancer Cells
Methods to Test Kinase Activity
Profiling Gene Expression Pattern
Transcriptional Control of ATG Genes
Analysis of Transcriptional Multiprotein Complexes
Novel Methods for Measuring Cardiac Autophagy In Vivo / Cynthia N. Perry ; Shiori Kyoi ; Nirmala Hariharan ; Hiromitsu Takagi ; Junichi Sadoshima ; Roberta A. Gottlieb16：
In Vivo Models of Autophagy in the Myocardium
Discussion
Autophagy in Load-Induced Heart Disease / Hongxin Zhu ; Beverly A. Rothermel ; Joseph A. Hill17：
Mouse Models of Load-Induced Heart Disease
Analysis of Ventricular Remodeling
In Vitro Models of Load-Induced Hypertrophy
Techniques to Analyze Cardiomyocyte Autophagy
Immunohistochemistry for LAMP-1 or Cathepsin D to Monitor Changes in Lysosomal Abundance
Isolation of LC3 Proteins from NRVM in Culture
Isolation of LC3 Protein from Heart of Skeletal Muscle Tissue
Soluble/Insoluble Fractionation of NRVM
Perspective
Evaluation of Cell Death Markers in Severe Calcified Aortic Valves / Wilhelm Mistiaen ; Michiel Knaapen18：
Clinical Importance of Degenerative Aortic Valve Disease
Pathological Appearances of AVD
Mechanism of Progression of AVD
Autophagy: Major Player in the Progression of Aortic Valve Disease?
Methods for Detection of Cell Death Markers in the Degenerated Aortic Valve
Methods for Quantification of Calcified Aortic Valves
Prospects and Concluding Remarks
Monitoring Autophagy in Muscle Diseases / May Christine V. Malicdan ; Satoru Noguchi ; Ichizo Nishino19：
Histological Observation of Skeletal Muscles
Measuring Autophagy by Protein Quantification
Monitoring Autophagy in Cultured Skeletal Myocytes
Electron Microscopy Observation of Skeletal Muscles
Immunoelectron Microscopy
Analyzing Macroautophagy in Hepatocytes and the Liver / Wen-Xing Ding ; Xiao-Ming Yin20：
The Pathophysiological Relevance of Macroautophagy in the Liver
Analysis of Autophagy in Isolated Hepatocytes
Analysis of Autophagy in the Liver
Summary
Monitoring Autophagy in Lysosomal Storage Disorders / Nina Raben ; Lauren Shea ; Victoria Hill ; Paul Plotz21：
General Techniques to Monitor Autophagy In LSDs
LSDs Analyzed for Autophagic Involvement
Author Index
Subject Index
Contributors
Preface
Volumes in Series
3.

電子ブック
ＥＢ
3. Autophagy in Mammalian Systems
Daniel; Klionsky, Daniel Klionsky, Daniel J. Klionsky, Daniel Klionsky
出版情報: Elsevier ScienceDirect Books Complete , Academic Press, 2009
所蔵情報: loading…
目次情報: 続きを見る
LC3-based assay for monitoring autophagy / Shunsuke Kimura ; Naonobu Fujita ; Takeshi Noda ; Tamotsu YoshimoriChapter 1：
Analysis of autophagy using GFP-LC3 transgenic mice / Noboru MizushimaChapter 2：
Photoactivatable GFP / Jennifer Lippincott-Schwartz ; Dale HaileyChapter 3：
Assaying for autophagic protein degradation / Fred MeijerChapter 4：
Sequestration assays for mammalian autophagy / Per O. SeglenChapter 5：
Incorporation of monodansylcadaverine (MDC) as an assay to assess autophagy induction and monitoring fusion with a degradative compartment / Maria Isabel ColomboChapter 6：
The GST-BHMT assay and related assays for autophagy / Carol A. Mercer ; Patrick B. DennisChapter 7：
Redox and autophagy-ROS as an indicator for autophagic activity / Ruth Scherz-Shouval ; Zvulun ElazarChapter 8：
FACS analysis of autophagy / Elena Shvets ; Ephraim FassChapter 9：
Electron microscopy in mammals / PSivi YlS-Anttila ; Eeva-Liisa EskelinenChapter 10：
Monitoring mTOR activity / Ken InokiChapter 11：
Using p62 as a marker for autophagy / Terje JohansenChapter 12：
Cytosolic LC3 ratio as a quantitative index of macroautophagy / Motoni Kadowaki ; Md. Razaul KarimChapter 13：
Pexophagy in mammalian cells / Junji Ezaki ; Takashi Ueno ; Eiki Kominami ; Masaaki KomatsuChapter 14：
Mitophagy in mammalian cells / Ji Zhang ; Paul A. NeyChapter 15：
Quantification of WIPI-1/Atg18 puncta formation for assessing mammalian autophagy / Tassula Proikas-Cezanne ; Simon G. PfistererChapter 16：
Correlative light and electron microscopy (CLEM) using GFP-LC3 and other autophagosome markers / Sharon Tooze ; Minoo RaziChapter 17：
Nanotechnology assays for autophagy / Oleksandr SeleverstovChapter 18：
Photoconvertible KAEDA-LC3 for monitoring autophagic flux / Christoph Goemans ; Aviva TolkovskyChapter 19：
Methods to monitor Chaperone-mediated autophagy / Ana Maria Cuervo ; Susmita KaushikChapter 20：
Autophagy in response to Salmonella enterica serovar Typhimurium infection / Cheryl Birmingham ; John BrumellChapter 21：
Monitoring autophagy during Mycobacterium tuberculosis infection / Vojo DereticChapter 22：
Shigella, Listeria and Streptococcus induction of autophagy / Michinaga Ogawa ; Yuko Yoshikawa ; Ichiro Nakagawa ; Chihiro Sasakawa ; Trinad ChakrabortyChapter 23：
Measuring the impact of infection on autophagy in macrophages / Jean-Francois Dubuisson ; Brenda Byrne ; Michele SwansonChapter 24：
LC3-based assay for monitoring autophagy / Shunsuke Kimura ; Naonobu Fujita ; Takeshi Noda ; Tamotsu YoshimoriChapter 1：
Analysis of autophagy using GFP-LC3 transgenic mice / Noboru MizushimaChapter 2：
Photoactivatable GFP / Jennifer Lippincott-Schwartz ; Dale HaileyChapter 3：
4.

電子ブック
ＥＢ
4. Autophagy in Disease and Clinical Applications
Daniel; Klionsky, Daniel Klionsky, Daniel J. Klionsky, Daniel Klionsky
出版情報: Elsevier ScienceDirect Books Complete , Academic Press, 2009
所蔵情報: loading…
目次情報: 続きを見る
Contributors
Preface
Volumes in Series
Initiation of Autophagy by Photodynamic Therapy / David Kessel ; Nancy L. Oleinick1：
Introduction
Photosensitizing Agents / 2：
Additional Factors Unique to PDT / 3：
A Typical PDT Protocol / 4：
Identification and Characterization of Autophagy after PDT / 5：
Effects of Autophagy on PDT Responses / 6：
Acknowledgments
References
Autophagic Cell Death / Michael J. Lenardo ; Christina K. McPhee ; Li Yu
Methods to Quantify Cell Death
Methods to Measure Autophagy
Methods to Establish Autophagy as the Cause of Cell Death
Autophagy Genes for RNAi Silencing
Transfact RNAi by Electroporation (Amaxa Nucleofection)
Conclusion / 7：
Autophagic Neuron Death / Yasuo Uchiyama ; Masato Koike ; Masahiro Shibata ; Mitsuho Sasaki
Experimental Models of Neurodegeneration
Measurements of Neuron Death
Assessing Metabolic Stress and Autophagy Status in Epithelial Tumors / Robin Mathew ; Vassiliki Karantza-Wadsworth ; Eileen White
Mouse Epithelial Cell Models for Studying the Role of Autophagy in Cancer
Protocols for Monitoring Autophagy in iBMK Cells and iMMECs In Vitro
Protocols for Monitoring Autophagy in Tumors In Vivo
Monitoring Chromosomal Instability Due to Autophagy Defects
Concluding Remarks and Future Perspectives
Autophagic Clearance of Aggregate-Prone Proteins Associated with Neurodegeneration / Sovan Sarkar ; Brinda Ravikumar ; David C. Rubinsztein
Aggregate-Prone Intracytoplasmic Proteins Associated with Neurodegenerative Disorders are Autophagy Substrates
Assays for the Clearance of Aggregate-Prone Proteins
Measurement of Autophagic Flux Using Bafilomycin A1
Concluding Remarks
Acknowledgements
Monitoring Autophagy in Alzheimer's Disease and Related Neurodegenerative Diseases / Dun-Sheng Yang ; Ju-Hyun Lee ; Ralph A. Nixon
General Approaches to Investigations of Human Neurodegeneration
Characterization of Autophagic Vacuoles, Evaluation of Autophagosome and Autolysosome Formation, and Autolysosomal Clearance
Metabolic Analyses of Autophagy in Neuronal and Nonneuronal Cell Models
Isolation and Characterization of Autophagic Vacuoles and Lysosomes from Cell Cultures and Brain Tissue
Western Blot Analysis of Autophagy Components and Substrates
Live-Cell Imaging of Autophagy Induction and Autophagosome-Lysosome Fusion in Primary Cultured Neurons / Mona Bains ; Kim A. Heidenreich
Cultured Cerebellar Purkinje Neurons as a Model to Study Neuronal Autophagy
Characterization of Autophagic Vacuole Size and Number in Purkinje Neurons
Using Colocalization of Fluorescent Tags to Measure Autophagosome-Lysosome Fusion
Using Genetic Mouse Models to Study the Biology and Pathology of Autophagy in the Central Nervous System / Zhenyu Yue ; Gay R. Holstein ; Brian T. Chait ; Qing Jun Wang8：
Methods
Analysis of GFP-LC3 Expression and Subcellular Localization in the CNS
Analysis of p62/SQSTM1 and Ubiquitinated Protein Inclusions in the CNS
Transmission Electron Microscopy (TEM) Analysis of Autophagosomes
Biochemical and Morphological Detection of Inclusion Bodies in Autophagy-Deficient Mice / Satoshi Waguri ; Masaaki Komatsu9：
Detection of Ubiquitinated Proteins and p62 in Autophagy-Deficient Mice by Western Blot Analysis
Detection of Ubiquitinated Proteins and p62 in Cultured Hepatocytes Derived from Autophagy-Deficient Mice
Detection of Ubiquitin-and p62-Positive Inclusions at the Light Microscopy Level
Detection of Ubiquitin-and p62-Positive Inclusions at the Electron Microscopy Level
Analyzing Autophagy in Clinical Tissues of Lung and Vascular Diseases / Hong Pyo Kim ; Zhi-Hua Chen ; Augustine M. K. Choi ; Stefan W. Ryter10：
Methods for Preparation of Lung and Vascular Cells
Analysis of Autophagy
Chromatin Immunoprecipitation
Conclusions
Autophagy in Neurite Injury and Neurodegeneration: In Vitro and In Vivo Models / Charleen T. Chu ; Edward D. Plowey ; Ruben K. Dagda ; Robert W. Hickey ; Salvatore J. Cherra ; Robert S. B. Clark11：
Studying Neuronal Autophagy In Vitro
Studying Brain Autophagy In Vivo
Future Perspectives and Challenges
Monitoring the Autophagy Pathway in Cancer / Frank C. Dorsey ; Meredith A. Steeves ; Stephanie M. Prater ; Thomas Schröter ; John L. Cleveland12：
LC3: A Phenotypic and Functional Marker of Autophagy
Assessing the Role of Autophagy in E&umu;-Myc-Driven Lymphoma
Autophagy Pathways in Glioblastoma / Hong Jiang ; Erin J. White ; Charles Conrad ; Candelaria Gomez-Manzano ; Juan Fueyo13：
Introduction: Autophagy and Gliomas
Prioritization of Methods to Characterize Autophagy in Gliomas
In Vitro Cellular Markers
In Vitro Biochemical Markers
Electron Microscopy to Monitor the Autophagic Vacuoles
In Vivo Analysis of Biochemical Markers
Autophagy Indicators as Surrogate Markets of Treatment Effect in Clinical Trials
Future Directions
Autophagy in Lung Cancer / Jerry J. Jaboin ; Misun Hwang ; Bo Lu14：
Signal-Dependant Control of Autophagy-Related Gene Expression / Fulvio Chiacchiera ; Cristiano Simone15：
Overview: Signal Transduction and Chromatin-Associated Kinases
The p38 Pathway in Colorectal Cancer Cells
Methods to Test Kinase Activity
Profiling Gene Expression Pattern
Transcriptional Control of ATG Genes
Analysis of Transcriptional Multiprotein Complexes
Novel Methods for Measuring Cardiac Autophagy In Vivo / Cynthia N. Perry ; Shiori Kyoi ; Nirmala Hariharan ; Hiromitsu Takagi ; Junichi Sadoshima ; Roberta A. Gottlieb16：
In Vivo Models of Autophagy in the Myocardium
Discussion
Autophagy in Load-Induced Heart Disease / Hongxin Zhu ; Beverly A. Rothermel ; Joseph A. Hill17：
Mouse Models of Load-Induced Heart Disease
Analysis of Ventricular Remodeling
In Vitro Models of Load-Induced Hypertrophy
Techniques to Analyze Cardiomyocyte Autophagy
Immunohistochemistry for LAMP-1 or Cathepsin D to Monitor Changes in Lysosomal Abundance
Isolation of LC3 Proteins from NRVM in Culture
Isolation of LC3 Protein from Heart of Skeletal Muscle Tissue
Soluble/Insoluble Fractionation of NRVM
Perspective
Evaluation of Cell Death Markers in Severe Calcified Aortic Valves / Wilhelm Mistiaen ; Michiel Knaapen18：
Clinical Importance of Degenerative Aortic Valve Disease
Pathological Appearances of AVD
Mechanism of Progression of AVD
Autophagy: Major Player in the Progression of Aortic Valve Disease?
Methods for Detection of Cell Death Markers in the Degenerated Aortic Valve
Methods for Quantification of Calcified Aortic Valves
Prospects and Concluding Remarks
Monitoring Autophagy in Muscle Diseases / May Christine V. Malicdan ; Satoru Noguchi ; Ichizo Nishino19：
Histological Observation of Skeletal Muscles
Measuring Autophagy by Protein Quantification
Monitoring Autophagy in Cultured Skeletal Myocytes
Electron Microscopy Observation of Skeletal Muscles
Immunoelectron Microscopy
Analyzing Macroautophagy in Hepatocytes and the Liver / Wen-Xing Ding ; Xiao-Ming Yin20：
The Pathophysiological Relevance of Macroautophagy in the Liver
Analysis of Autophagy in Isolated Hepatocytes
Analysis of Autophagy in the Liver
Summary
Monitoring Autophagy in Lysosomal Storage Disorders / Nina Raben ; Lauren Shea ; Victoria Hill ; Paul Plotz21：
General Techniques to Monitor Autophagy In LSDs
LSDs Analyzed for Autophagic Involvement
Author Index
Subject Index
Contributors
Preface
Volumes in Series
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