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1.

図書
図書
1. Ras family
edited by W.E. Balch, Channing J. Der, Alan Hall
出版情報: San Diego ; Tokyo : Academic Press, c1995  xxxi, 548 p. ; 24 cm
シリーズ名: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 255 . Small GTPases and their regulators ; pt. A
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Expression, Purification, and Posttranslational Modification / S.L. Campbell-Burk ; J.W. Carpenter
Refolding and Purification of Ras Proteins / E. Porfiri ; T. Evans ; G.E. Bollag ; R. Clark ; J.F. Hancock
Purification of Baculovirus-Expressed Recombinant Ras and Rap Proteins / F. McCormick
Purification of Recombinant Ras GTPase-Activating Proteins / B. Rubinfeld ; P. Polakis
Purification of Baculovirus-Produced Rap 1 GTPase-Activating Proteins / G.L. James ; M.S. Brown ; J.L. Goldstein
Assays for Inhibitors for CAAX Farnesyltransferase in Vitro and in Intact Cells / R. Khosravi-Far ; C.J. Der
Prenylation Analysis of Bacterially Expressed and Insect Cell-Expressed Ras and Ras-Related Proteins
Reticulocyte Lysate Assay for in Vitro Translation and Posttranslational Modification of Ras Proteins / C. Volker ; J.B. Stock
Carboxyl Methylation of Ras-Related Proteins / F. Tamanoi ; H. Mitsuzawa
Use of Yeast for Identification of Farnesyltransferase Inhibitors and for Generation of Mutant Farnesyltransferases
Guanine Nucleotide Exchange and Hydrolysis / C. Lenzen ; R.H. Cool ; A. Wittinghofer
Analysis of Intrinsic and CDC25-Stimulated Guanine Nucleotide Exchange of p21 ras --Nucleotide Complexes by Fluorescence Measurements / J. Downward
Measurement of Nucleotide Exchange and Hydrolysis Activities in Immunoprecipitates / J.B. Gibbs
Determination of Guanine Nucleotides Bound to Ras in Mammalian Cells / M. Frech ; D. Cussac ; P. Chardin ; D. Bar-Sagi
Purification of Baculovirus-Expressed Human Sos1 Protein / M. Hart ; S. Powers
Ras-Cdc25 and Rho-Dbl Binding Assays: Complex Formation in Vitro / R.D. Mosteller ; W. Park ; D. Broek
Analysis of Interaction between Ras and CDC25 Guanine Nucleotide Exchange Factor Using Yeast GAL4 Two-Hybrid System / T. Satoh ; Y. Kaziro
Measurement of Ras-Bound Guanine Nucleotide in Stimulated Hematopoietic Cells / A.M.M. de Vries-Smits ; L. van der Voorn ; J.L. Bos
Measurements of GTP/GDP Exchange in Permeabilized Fibroblasts
Intrinsic and GTPase-Activating Protein-Stimulated Ras GTPase Assays / M.D. Schaber
Determination of Ras and GTPase-Activating Protein Interactions by Kinetic Competition Assay / C. Ellis ; V. Measday ; M.F. Moran
Phosphorylation-Dependent Complexes of p120 Ras-Specific GTPase-Activating Proteins with p62 and p190
Cell Expression and Analysis: in Vitro / A.D. Cox ; P.A. Solski ; J.D. Jordan
Analysis of Ras Protein Expression in Mammalian Cells / A.M.M. deVries-Smits ; B.M.T. Burgering
Vaccinia Virus Expression of p21ras Asn-17 / H. Cai ; G.M. Cooper
Inducible Expression of Ras N17 Dominant Inhibitory Protein
Prenylation and Palmitoylation Analysis / C.W.M. Reuter ; A.D. Catling ; M.J. Weber
Immune Complex Kinase Assays for Mitogen-Activated Protein Kinase and MEK / S. Kuroda ; K. Shimizu ; B. Yamamori ; Y. Takai
Cell-Free Assay System for Ras-Dependent MEK Activation / P. Dent ; G. Romero ; D. Castle ; T.W. Sturgill
Preparation and Use of Semiintact Mammalian Cells for Analysis of Signal Transduction / C.J
Expression, Purification, and Posttranslational Modification / S.L. Campbell-Burk ; J.W. Carpenter
Refolding and Purification of Ras Proteins / E. Porfiri ; T. Evans ; G.E. Bollag ; R. Clark ; J.F. Hancock
Purification of Baculovirus-Expressed Recombinant Ras and Rap Proteins / F. McCormick
2.

図書
図書
2. Rho family
edited by W.E. Balch, Channing J. Der, Alan Hall
出版情報: San Diego ; Tokyo : Academic Press, c1995  xxix, 401 p. ; 24 cm
シリーズ名: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 256 . Small GTPases and their regulators ; pt. B
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Expression, Purification, and Posttranslational Modification / A.J. Self ; A. Hall
Purification of Recombinant Rho/Rac/G25K fromEscherichia coli / R.A.Cerione ; D. Leonard ; Y. Zheng
Purification of Baculovirus-Expressed Cdc42Hs / T. Mizuno ; H. Nakanishi ; Y. Takai
Purification and Properties of Rac2 from Human Leukemia Cells / U.G. Knaus ; G.M. Bokoch
Purification of Rac2 from Human Neutrophils / A. Abo
Purification of Rac(GDI Dissociation Inhibitor Complex from Phagocyte Cytosol / K. Tanaka ; T. Sasaki
Purification and Properties of Recombinant Rho(GDP Dissociation Inhibitor / M.R. Philips ; M.H. Pillinger
Prenylcysteine-Directed Carboxyl Methyltransferase Activity in Human Neutrophil Membranes
Guanine Nucleotide Exchange and Hydrolysis
Measurement of Intrinsic Nucleotide Exchange and GTP Hydrolysis Rates / M.J. Hart ; R.A. Cerione
Guanine Nucleotide Exchange Catalyzed by dbl Oncogene Product / E. Porfiri ; J.F. Hancock
Stimulation of Nucleotide Exchange on Ras- and Rho-Related Proteins by Small GTP-Binding Protein GDP Dissociation Stimulator / T. Miki
Interaction of Ect2 and Dblwith Rho-related GTPases / D.A. Leonard
Solubilization of Cdc42Hs from Membranes by Rho(GDP Dissociation Inhibitor / J. Settleman ; R. Foster
Purification and GTPase-Activating Protein Activity of Baculovirus Expressed p190 / S. Ahmed ; R. Kozma ; C. Hall ; L. Lim
GTPase-Activating Protein Activity of n(alpha1)-Chimaerin and Effect of Lipids / D.E.H. Afar ; O.N. Witte
Characterization of Breakdown Cluster Region Kinase and SH2-Binding Activities / E. Manser ; T. Leung
Identification of GTPase-Activating Proteins by Nitrocellulose Overlay Assay / P. Cicchetti ; D. Baltimore
Identification of 3BP-1 in cDNA Expression Library by SH3 Domain Screening
Cell Expression and in Vitro Analysis / P. Fort ; S. Vincent
Serum Induction of RhoG Expression / H. Paterson ; P. Adamson ; D. Robertson
Microinjection of Epitope-Tagged Rho Family cDNAs and Analysis by Immunolabeling / S.T. Dillon ; L.A.Feig
Purification and Assay of Recombinant C3 Transferase / K. Aktories ; I. Just
In Vitro ADP-Ribosylation of Rho by Bacterial ADP-Ribosyltransferases / N. Morii ; S. Narumiya
Preparation of Native and Recombinant Clostridium botulinum C3 ADP-Ribosyltransferase and Identification of Rho Proteins by ADP-Ribosylation / D. Diekmann
In Vitro Binding Assay for Interactions of Rho and Rac with GTPase-Activating Proteins and Effectors
Purification and Assay of Kinases That Interact with Rac/Cdc42 / P. Aspenstrim ; M.F. Olson
Yeast Two-Hybrid System to Detect Protein(Protein Interactions with Rho GTPases / S.E. Rittenhouse
Assay for Rho-Dependent Phosphoinositide 3-Kinase Activity in Platelet Cytosol / E.P. Bowman ; D.J. Uhlinger ; J.D. Lambeth
Neutrophil Phospholipase D: Inhibition by Rho(GDP Dissociation Inhibitor and Stimulation by Small GTPase GDP Dissociation Stimulator / M.T. Quinn
Measurement of Rac Translocation from Cytosol to Membranes in Activated Neutrophils / A.W. Segal
Reconst
Expression, Purification, and Posttranslational Modification / A.J. Self ; A. Hall
Purification of Recombinant Rho/Rac/G25K fromEscherichia coli / R.A.Cerione ; D. Leonard ; Y. Zheng
Purification of Baculovirus-Expressed Cdc42Hs / T. Mizuno ; H. Nakanishi ; Y. Takai
3.

図書
図書
3. Proteins involved in transport
edited by W.E. Balch, Channing J. Der, Alan Hall
出版情報: San Diego ; Tokyo : Academic Press, c1995  xxix, 360 p. ; 24 cm
シリーズ名: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 257 . Small GTPases and their regulators ; pt. C
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Expression, Purification, and Posttranslational Modification / C. Nuoffer ; F. Peter ; W.E. Balch
Purification of His6-Tagged Rab1 Proteins Using Bacterial and Insect Cell Expression Systems / H. Horiuchi ; O. Ullrich ; C. Bucci ; M. Zerial
Purification of Posttranslationally Modified and Unmodified Rab5 Protein Expressed in Spodoptera frugiperda Cells / M.A. Riederer ; T. Soldati ; A.B. Dirac-Svejstrup ; S.R. Pfeffer
Expression of Rab9 Protein in Escherichia coli: Purification and Isoprenylation in Vitro / Y. Jiang ; G. Rossi ; S. Ferro-Novick
Characterization of Yeast Type-II Geranylgeranyltransferase / S.A. Armstrong ; M.S. Brown ; J.L. Goldstein ; M.C. Seabra
Preparation of Recombinant Rab Geranylgeranyltransferase and Rab Escort Proteins / K. Kimura ; T. Oka ; A. Nakano
Purification and Assay of Yeast Sar1p / T. Rowe
Expression and Purification of Mammalian Sar1
Guanine NucleotideExchange and Hydrolysis / A. Kikuchi ; H. Nakanishi ; Y. Takai
Purification and Properties of Rab3A / T. Sasaki
Purification and Properties of Bovine Rab-GDP Dissociation Inhibitor / I. Schalk
Expression and Purification of Recombinant His6-Tagged Guanine Nucleotide Dissociation Inhibitor and Formation of Rab1 Complex / D.M. Roberts ; M.D. Garrett ; P.J. Novick
Purification of GDP Dissociation Stimulator Dss4 from Recombinant Bacteria / J.L. Burton ; P. De Camilli
Expression, Purification, and Functional Assay of Mss4 / C. Barlowe ; R. Schekman
Expression, Purification, and Assay of Sec12p: A Sar1p-Specific GDP Dissociation Stimulator / I.G. Macara ; W.H. Brondyk
Oligonucleotide Mutagenesis of Rab GTPases / P. Vollmer ; D. Gallwitz
High Expression Cloning, Purification, and Assay of Ypt-GTPase Activating Proteins / P.A. Randazzo ; O. Weiss ; R.A. Kahn
Preparation of Recombinant ADP-Ribosylation Factor / F.R. Bischoff ; H. Ponstingl
Catalysis of Guanine Nucleotide Exchange of Ran by RCC1 and Stimulation of Hydrolysis of Ran-Bound GTP by Ran-GAP1 / T. Yeung ; T. Yoshihisa
Purification of Sec23p-Sec24p Complex. Cell Expression and Analysis in Vivo / H. Stenmark
Expression of Rab GTPase Using Recombinant Vaccinia Viruses / C. Dascher ; E.J. Tisdale
Transient Expression of Small GTPases to Study Protein Transport along Secretory Pathway in Vivo Using the Recombinant T7 Vaccinia Virus System / J.K. VanSlyke ; L. Thomas ; G. Thomas
Preparation of Recombinant Vaccinia Virus for Expression of Small GTPases / L. Martin-Parras
Using Oligonucleotides for Cloning Rab Proteins by PCR
Use of Two-Hybrid System to Identify Rab-Binding Proteins / H. Damke ; M. Gossen ; S. Freundlieb ; H. Bujard ; S.L. Schmid
Tightly Regulated and Inducible Expression of Dominant Interfering Dynamin Mutant in Stably Transformed HeLa Cells / R.W. Holz ; R.A. Senter ; M.D. Uhler
Investigation by Transient Transfection of the Effects on Regulated Exocytosis of Rab3A
Expression, Purification, and Assays of Gdi1p from Recombinant Escherichia coli
Biological Activity
Expression, Purification, and Posttranslational Modification / C. Nuoffer ; F. Peter ; W.E. Balch
Purification of His6-Tagged Rab1 Proteins Using Bacterial and Insect Cell Expression Systems / H. Horiuchi ; O. Ullrich ; C. Bucci ; M. Zerial
Purification of Posttranslationally Modified and Unmodified Rab5 Protein Expressed in Spodoptera frugiperda Cells / M.A. Riederer ; T. Soldati ; A.B. Dirac-Svejstrup ; S.R. Pfeffer
4.

図書
図書
4. Rho family
edited by W.E. Balch, Channing J. Der, Alan Hall
出版情報: San Diego ; Tokyo : Academic Press, c2000  xxxv, 547 p., [1] leaf of plate ; 24 cm
シリーズ名: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 325 . Regulators and effectors of small GTPases ; pt. D
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Contributors to Volume 325
Preface
Volumes in Series
Purification, Posttranslational Modification, and in Vitro Regulation / Section I.：
Purification and Biochemical Characterization of TC10 / Cheryl L. Neudauer ; Ian G. Macara1.：
Expression and Purification of Rho/RhoGDI Complexes / Paul W. Read ; Robert K. Nakamoto2.：
Bacterial Expressed DH and DH/PH Domains / Kent L. Rossman ; Sharon L. Campbell3.：
Biochemical Analysis of Regulation of Vav, a Guanine-Nucleotide Exchange Factor for Rho Family of GTPases / Raymond Mosteller ; Jaewon Han ; Balaka Das ; Daniel Broek4.：
Activation of Rac1 by Human Tiam1 / Gideon Bollag ; Anne M. Crompton ; Danielle ; Peverly-Mitchell ; Gaston G. M. Habets ; Marc Symons5.：
Activation of Rho GEF Activity by G[alpha subscript 13] / Matthew J. Hart ; William Roscoe6.：
Purification and Characterization of Guanine Nucleotide Dissociation Stimulator Protein / Jon P. Hutchinson ; Katrin Rittinger ; John F. Eccleston7.：
Purification and Biochemical Activity of Salmonella Exchange Factor SopE / Andrea Friebel ; Wolf-Dietrich Hardt8.：
Stimulation of Rho GDI Release by ERM Proteins / Akiko Mammoto ; Kazuo Takahashi ; Takuya Sasaki ; Yoshimi Takai9.：
Expression and Activity of Human Prenylcysteine-Directed Carboxyl Methyltransferase / Edwin Choy ; Mark Philips10.：
Purification and Evaluation of Large Clostridial Cytotoxins That Inhibit Small GTPases of Rho and Ras Subfamilies / Michael Moos ; Christoph von Eichel-Streiber11.：
Rho GTPase-Activating Toxins: Cytotoxic Necrotizing Factors and Dermonecrotic Toxin / Gudula Schmidt ; Klaus Aktories12.：
Purification and Activity of GTPase Targets / Section II.：
Measurement of GTPase Effector Affinities / Danny Manor13.：
Purification and in Vitro Activity of Rho-Associated Kinase / Mutsuki Amano ; Yuko Fukata ; Hiroaki Shimokawa ; Kozo Kaibuchi14.：
Purification and in Vitro Activities of p21-Activated Kinases / Charles C. King ; Abina M. Reilly ; Ulla G. Knaus15.：
Stimulation of Phospholipase C-[beta subscript 2] by Rho GTPases / Daria Illenberger ; Ilona Stephan ; Peter Gierschik ; Frieder Schwald16.：
Regulation of Phospholipase D1 Activity by Rho GTPases / Michael A. Frohman ; Yasunori Kanaho ; Yue Zhang ; Andrew J. Morris17.：
In Vitro Interaction of Phosphoinositide-4-phosphate 5-Kinases with Rac / Kimberley Tolias ; Christopher L. Carpenter18.：
Activity and Regulation of p35/Cdk5 Kinase Complex / Margareta Nikolic ; Li-Huei Tsai19.：
Actin Assembly Mediated by Arp2/3 Complex and WASP Family Proteins / R. Dyche Mullins ; Laura M. Machesky20.：
In Vitro Actin Polymerization Using Polymorphonuclear Leukocyte Extracts / Sally H. Zigmond21.：
Analysis of Rho GTPase Function / Section III.：
Determination of GTP Loading on Rac and Cdc42 in Platelets and Fibroblasts / Anser C. Azim ; Kurt L. Barkalow ; John H. Hartwig22.：
Determination of GTP Loading on Rho / Xiang-Dong Ren ; Martin Alexander Schwartz23.：
Use and Properties of ROCK-Specific Inhibitor Y-27632 / Shuh Narumiya ; Toshimasa Ishizaki ; Masayoshi Uehata24.：
Inducible Membrane Recruitment of Small GTP-Binding Proteins by Rapamycin-Based System in Living Cells / Flavia Castellano ; Philippe Chavrier25.：
Expression of Rho GTPases Using Retroviral Vectors / Frits Michiels ; Rob A. van der Kammen ; Lennert Janssen ; Garry Nolan ; John G. Collard26.：
Expression of Rho GTPases Using Adenovirus Vectors / Daniel M. Sullivan ; Toren Finkel27.：
In Vivo Activity of Wild-Type and Mutant PAKs / Luraynne C. Sanders ; Gary M. Bokoch28.：
Single Cell Assays for Rac Activity / Laura J. Taylor ; Amy B. Walsh ; Patrick Hearing ; Dafna Bar-Sagi29.：
Effect of Rho GTPases on Na-H Exchanger in Mammalian Cells / Sheryl P. Denker ; Weihong Yan ; Diane L. Barber30.：
Actin Filament Assembly in Permeabilized Platelets / 31.：
Rho GTPases: Secretion and Actin Dynamics in Permeabilized Mast Cells / Mark R. Holt ; Anna Koffer32.：
Isolation and in Vitro Contraction of Stress Fibers / Kazuo Katoh ; Yumiko Kano ; Keigi Fujiwara33.：
Inhibition of Rho GTPases Using Protein Geranylgeranyltransferase I Inhibitors / Said M. Sebti ; Andrew D. Hamilton34.：
Imaging Spatiotemporal Dynamics of Rac Activation in Vivo with FLAIR / Chester E. Chamberlain ; Vadim S. Kraynov ; Klaus M. Hahn35.：
Biological Assays of Rho GTPase Function / Section IV.：
Rho-like GTPases in Tumor Cell Invasion / Eva E. Evers ; Jean P. ten Klooster36.：
Mouse Embryo Fibroblasts: A Genetic Model System for Studying Rho- and Ras-Dependent Cell Cycle Progression / Giovanna M. D'Abaco ; Michael F. Olson37.：
Analyses of Transforming Activity of Rho Family Activators / Patricia A. Solski ; Karon Abe ; Channing J. Der38.：
Rho GTPases and Cell Migration-Fibroblast Wound Healing / Catherine D. Nobes39.：
Rho GTPases and Cell Migration: Measurement of Macrophage Chemotaxis / Gareth E. Jones ; Anne J. Ridley ; Daniel Zicha40.：
Rho GTPases and Macrophage Phagocytosis / Jayesh C. Patel ; Alan Hall ; Emmanuelle Caron41.：
Rho GTPases and Axonal Growth Cone Collapse / Alyson E. Fournier ; Robert G. Kalb ; Stephen M. Strittmatter42.：
Study of in Situ Function of Cytoskeletal Proteins in Lamellipodia and Filopodia Using Microscale Chromophore-Assisted Laser Inactivation / Eric V. Wong ; Daniel G. Jay43.：
Modulation of Actin Cytoskeleton by Salmonella GTPase Activating Protein SptP / Jorge E. Galan ; Yixin Fu44.：
Author Index
Subject Index
Contributors to Volume 325
Preface
Volumes in Series
5.

図書
図書
5. GTPases involved in vesicular traffic
edited by W.E. Balch, Channing J. Der, Alan Hall
出版情報: San Diego ; Tokyo : Academic Press, c2001  xxxviii, 553 p., [3] leaves of plates ; 24 cm
シリーズ名: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 329 . Regulators and effectors of small GTPases ; pt. E
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6.

図書
図書
6. Ras family (1 ; 2)
edited by W.E. Balch, Channing J. Der, Alan Hall
出版情報: San Diego ; Tokyo : Academic Press, c2001  v. ; 24 cm
シリーズ名: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 332-333 . Regulators and effectors of small GTPases ; pt. F-G
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7.

図書
図書
7. Rho family
edited by William E. Balch, Channing J. Der, Alan Hall
出版情報: Amsterdam ; San Diego ; Tokyo : Elsevier Academic Press, c2006  xliii, 798 p., [24] p. of plates ; 24 cm
シリーズ名: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 406 . Regulators and effectors of small GTPases
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Purification and Biochemical Properties of Rac1,2,3 and the Splice Variant Rac1b
Biochemical Analyses of the Wrch Atypical Rho Family GTPases
Purification of P-Rex1 from neutrophils and nucleotide exchange assay
In vitro guanine nucleotide exchange activity of DHR-2/DOCKER/ CZH2 domains
Biochemical characterization of the Cool (Coned-out-of-Library)/Pix (Pak-interactive exchange factor) proteins
GEF and glucosylation assays on liposome bound Rac
Phosphorylation of RhoGDI by p21-activated kinase 1
Purification of ARAP3 and characterization of GAP activities
Regulation of RhoGAP specificity by phospholipids and prenylation
Purification and activity of the Rho ADP-ribosylating binary C2/C3 toxin
Purification of Tat-C3 exoenzymes
Imaging and photobleach correction of MeroCBD, sensor of endogenous Cdc42 activation
Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts
In Vitro Reconstitution of Cdc42-Mediated Actin Assembly Using Purified Components
Biochemical Analysis of Mammalian Formin Effects on Actin Dynamics
Formin proteins: purification and measurement of effects on actin assembly
Purification and Kinase Assay of PKN
Purification and Enzyme Activity of ACK1
Direct activation of purified phospholipase C epsilon by RhoA studied in reconstituted phospholipid vesicles
Regulation of PLCG isoforms by Rac
Biochemical properties and inhibitors of (N-)WASP
The Use of GFP to localize Rho GTPases in Living Cells
Analysis of the Spatio-Temporal Activation of Rho GTPases using Raichu Probes
Measurement of Activity of Rho GTPases during Mitosis
RNAi inhibition of Rho GTPases
RNA interference techniques to study epithelial cell adhesion and polarity
Nucleofection of primary neurons
Dock180-ELMO Cooperation in Rac Activation
Rho GTPase activation by cell-cell adhesion
Activation of Rap1, Cdc42, and Rac by nectin adhesion system
Analysis of activated GAPs and GEFs in cell lysates
Degradation of RhoA by Smurf1 ubiquitin ligase
Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin
Regulation of superoxide-producing NADPH in non-phagocytic cells
Activation of MEKK1 by Rho GTPases
Activation of Apoptotic JNK Pathway through the Rac1-Binding Scaffold Protein POSH
Quantification of isozyme-specific activation of phospholipase C-G2 by Rac GTPases and phospholipase C-s by Rho GTPases in an intact cell assay system
Activation of Rho and Rac by Wnt/Frizzled signalling
Fluorescent assay of cell permeable C3 transferase activity
Use of TIRF microscopy to visualize actin and microtubules in migrating cells
Inhibition of ROCK by RhoE
Conditional regulation of a ROCK-estrogen receptor fusion protein
Rational design and applications of a Rac GTPase-specific small molecule inhibitor
In vitro assay of primary astrocyte migration as a tool to study Rho GTPase function in cell polarization
Real time centrosome reorientation during fibroblast migration
Lentiviral delivery of RNAi in Hippocampal Neurons
Methods for studying neutrophil Chemtoaxis
Measurement of Epidermal Growth Factor Receptor turnover and effects of Cdc42
Tumor cell migration in three dimensions
Reciprocal regulation of cyclin D1 expression by Rac and Rho
Regulation of ERK signal duration and cyclin D1 expression by Rhodependent stress fiber formation
An in vitro model to study the role of endothelial Rho GTPases during leukocyte transendothelial migration
Analysis of a Mitotic role of Cdc42
Plexin-induced collapse assay in COS cells
Morphological and Biochemical Analysis of Rac1 in Three-Dimensional Epithelial Cell Cultures
Using Three Dimensional Acinar Structures for Molecular and Cell Biological Assays
TC10 and insulin-stimulated glucose transport
GTPases and the control of neuronal polarity
In vitro assembly of filopodia-like bundles
Purification and Biochemical Properties of Rac1,2,3 and the Splice Variant Rac1b
Biochemical Analyses of the Wrch Atypical Rho Family GTPases
Purification of P-Rex1 from neutrophils and nucleotide exchange assay
8.

図書
図書
8. Ras family
edited by William E. Balch, Channing J. Der, Alan Hall
出版情報: Amsterdam ; San Diego ; Tokyo : Academic Press, c2006  xliii, 780 p., [18] p. of plates ; 24 cm
シリーズ名: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 407 . Regulators and effectors of small GTPases
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Real-time in vitro measurement of Intrinsic and Ras GAP-mediated GTP hydrolysis
Schwann cell preparation from single mouse embryos: Analyses of neurofibromin function in Schwann cells
Regulation of the nucleotide state of oncogenic Ras proteins by nucleoside diphosphate kinase
Measurements of TSC2 GAP activity toward Rheb
Characterization of AND-34 function and signalling
Studying the spatial and temporal regulation of Ras GTPase-activating proteins
Activation of Ras Proteins by Ras Guanine Nucleotide Releasing Protein Family Members
Ras and Rap1 Activation of PLC? lipase activity
Specificity and expression of RalGPS as RalGEFs
Biochemical and biological analyses of Rgr RalGEF oncogene
Analysis of Ras Activation in Living Cells with GFP-RBD
Genetic and pharmacologic analyses of the role of Icmt in Ras membrane association and function
Characterization of the activation of the Rap specific exchange factor epac by cyclic nucleotides
Biochemistry of the Rap-specific guanine nucleotide exchange factors PDZ-GEF1 and 2
Characterization of interactions between Ras family GTPases and their effectors
Genetic and pharmacologic dissection of Ras effector utilization in oncogenesis
Sequencing analysis of BRAF mutations in human cancers
KSR regulation of the Raf-MEK-ERK cascade
Ras-sensitive IMP modulation of the Raf/MEK/ERK cascade through KSR1
Raf Kinase Inhibitor Protein (RKIP) regulation of Raf and MAPK signalling
Harnessing RNAi for Analyses of Ras Signaling and Transformation
The Rac Activator Tiam1and Ras induced Oncogenesis
Phospholipase Ce C+ guanine nucleotide exchange factor activity and activation of Rap1
Nore1 and RASSF1 regulation of cell proliferation and of the MST1/2 kinases
RASSF family proteins and Ras transformation
Ras and the Rain/RasBP1 effector
The RIN family of Ras effectors
RAP1 regulation of RIAM and cell adhesion
Regulation of cell-cell adhesion by Rap1
Effects of Ras signaling on gene expression analyzed by customized microrays
Protein-fragment complementation assays (PCA) in small GTPase research and drug discovery
Ras Up-regulation of Cycloxygenase-2
Regulation of the expression of tropomyosins and actin cytoskeleton by ras-transformation
Regulation of Par-4 by Oncogenic Ras
Using Drosophila and yeast genetics to investigate a role for the RhebGTPase in cell growth
Biochemistry and Biology of ARHI (DIRAS3), An Imprinted Tumor Suppressor Gene Whose Expression Is Lost In Ovarian and Breast Cancer
Gem protein signaling and regulation
Analysis of Rem/RGK Signaling and Biological Activity
Analysis of Rit Signaling and Biological Activity
Characterization of RERG: An estrogen-regulated tumor suppressor gene
Inhibition of transcription factor NF-?B activation by ?B-Ras
Analysis of Rhes activation state and effector function
Rheb activation of mTOR and S6K1 signaling
Use of Retrovirus Expression of Interfering RNA to Determine the Contribution of Activated K-Ras and Ras Effector Expression to Human Tumor Cell Growth
Using inhibitors of prenylation to block localization and transforming activity
Sorafenib (BAY 43-9006), a dual action inhibitor that targets RAF/MEK/ERK pathway in tumor cells and tyrosine kinases VEGFR/PDGFR in tumor vasculature
Yeast screens for inhibitors of Ras-Raf interaction and characterization of MCP inhibitors of Ras-Raf interaction
A tagging-via-substrate technology for genome-wide detection and identification of farnesylated proteins
A genetically defined normal human somatic cell system to study Ras oncogenesis in vivo and in vitro
Analyses of Ras transformation of human thyroid epithelial cells
Use of Ras-transformed human ovarian surface epithelial cells as a model for studying ovarian cancer
Physiological analysis of oncogenesic Kras
Use of conditionally active Ras Fusion Proteins to study Epidermal Growth, Differentiation and Neoplasia
Pancreatic duct epithelial cell isolation and cultivation in two dimensional and three dimensiona
Real-time in vitro measurement of Intrinsic and Ras GAP-mediated GTP hydrolysis
Schwann cell preparation from single mouse embryos: Analyses of neurofibromin function in Schwann cells
Regulation of the nucleotide state of oncogenic Ras proteins by nucleoside diphosphate kinase
9.

図書
図書
9. Small GTPases in disease (pt. A ; pt. B)
edited by William E. Balch, Channing J. Der, Alan Hall
出版情報: Amsterdam ; Tokyo : Elsevier Academic Press, c2008  2 v. ; 24 cm
シリーズ名: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 438-439
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Rab1b silencing using small interfering RNA for analysis of disease specific function / Darren M. Hutt ; William E. BalchChapter 1：
Rab8-optineurin-myosin VI: Analysis of interactions and functions in the secretory pathway / Margarita V. Chibalina ; Rhys C. Roberts ; Susan D. Arden ; John Kendrick-Jones ; Folma BussChapter 2：
Characterization of Rab27a and JFC1 as constituents of the secretory machinery of prostate specific antigen in prostate carcinoma cells / Sergio D. CatzChapter 3：
In vitro assays to characterize inhibitors of the activation of small G proteins by their guanine nucleotide exchange factors / Jean-Christophe Zeeh ; Bruno Antonny ; Jacqueline Cherfils ; Mahel ZeghoufChapter 4：
Analysis of small GTPase function in trypanosomes / Mark C. Field ; David Horn ; Mark CarringtonChapter 5：
Use of dynasore, the small molecule inhibitor of dynamin, in the regulation of endocytosis / Tom Kirchhausen ; Eric Macia ; Henry E. PelishChapter 6：
Identification and verification of Sro7p as an effector of the Sec4p rab GTPase / Bianka Grosshans1 ; Peter NovickChapter 7：
Characterization of Rab18, a Lipid Droplet Associated Small GTPase / Sally Martin ; Robert G. PartonChapter 8：
Analysis on the Emerging Role of of Rab3 GTPase-activating Protein in Warburg Micro and Martsolf Syndrome / Ayuko Sakane ; Jun Miyoshi ; Yoshimi Takai ; Takuya SasakiChapter 9：
Identification and Characterization of JRAB/MICAL-L2, a Junctional Rab13-binding Protein / Noriyuki NishimuraChapter 10：
A SMAP Gene Family Encoding ARF GTPase-activating Proteins and Its Implication in Membrane Trafficking / Kenji Tanabe ; Shunsuke Kon ; Nobuyuki Ichijo ; Tomo Funaki ; Waka Natsume ; Toshio Watanabe ; Masanobu SatakeChapter 11：
In vitro and in vivo analysis of neurotrophin-3 activation of Arf6 and Rac-1. / Pedro F. Esteban ; Paola Caprari ; Hye-Young Yoon ; Paul A. Randazzo ; Lino TessarolloChapter 12：
Methods for analysis of rab27a/munc13-4 in secretory lysosome release in haematopoietic cells / Peter van der Sluijs ; Maaike Neeft ; Thijs van Vlijmen ; Edo Elstak ; Marnix WiefferChapter 13：
Analysis and expression of Rab38 in Oculocutaneous / Lung Disease Kazuhiro Osanai ; Dennis R. VoelkerChapter 14：
Analysis of Rab1 function in cardiomyocyte growth / Guangyu Wu ; Catalin M. Filipeanu ; Fuguo ZhouChapter 15：
Regulation of the Trafficking and Function of G Protein-coupled Receptors by Rab1 GTPase in Cardiomyoctyes / Chapter 16：
Regulation of endosome dynamics by Rab5 and its Huntingtin-HAP40 effector complex in physiological versus pathological conditions / Arun Pal ; Fedor Severin ; Sebastian H÷pfner ; Marino ZeriaChapter 17：
Biochemical and Biophysical Analyses of Ras Modification by Ubiquitin / Sonia Terrillon ; Dafna Bar-SagiChapter 18：
Biochemical Characterization of Novel Germline BRAF and MEK Mutations in Cardio-facio-cutaneous Syndrome / Pablo Rodriguez-Viciana ; Katherine A. RauenChapter 19：
Biochemical and biological characterization of tumor-associated mutations of p110a / Adam Denley ; Marco Gymnopolous ; Jonatha R. Hart ; Hao Jiang ; Li Zhao ; Peter K. VogtChapter 20：
Characterization of the Rheb-mTOR signaling pathway in mammalian cells: Constitutive active mutants of Rheb and mTOR / Tatsuhiro Sato ; Akiko Umetsu ; Fuyuhiko TamanoiChapter 21：
Characterization of RalB-Sec5-TBK1 Function in Human Oncogenesis / Yuchen Chien ; Michael A. WhiteChapter 22：
High-throughput lung cancer cell line screening for genotype-correlated sensitivity to an EGFR kinase inhibitor / Ultan McDermott ; Sreenath V. Sharma ; Jeffrey SettlemanChapter 23：
In vitro signalling by MAPK and NFkB pathyways inhibited by Yersinia YopJ / Sohini Mukherjee ; Kim OrthChapter 24：
Biological a<$$$> / Chapter 25：
Rab1b silencing using small interfering RNA for analysis of disease specific function / Darren M. Hutt ; William E. BalchChapter 1：
Rab8-optineurin-myosin VI: Analysis of interactions and functions in the secretory pathway / Margarita V. Chibalina ; Rhys C. Roberts ; Susan D. Arden ; John Kendrick-Jones ; Folma BussChapter 2：
Characterization of Rab27a and JFC1 as constituents of the secretory machinery of prostate specific antigen in prostate carcinoma cells / Sergio D. CatzChapter 3：
10.

図書
図書
10. GTPases regulating membrane targeting and fusion
edited by William E. Balch, Channing J. Der, Alan Hall
出版情報: Amsterdam ; San Diego, Calif. ; Tokyo : Elsevier Academic Press, c2005  xliii, 863 p., [28] p. of plates ; 24 cm
シリーズ名: Methods in enzymology / editors in chief, Sidney P. Colowick, Nathan O. Kaplan ; v. 403
所蔵情報: loading…
目次情報: 続きを見る
Contributors to Volume 403
Preface
Volumes in Series
Exploring Trafficking GTPase Function by mRNA Expression Profiling: Use of the SymAtlas Web-Application and the Membrome Datasets / Cemal Gurkan ; Hilmar Lapp ; John B. Hogenesch ; William E. Balch1：
Use of Search Algorithms to Define Specificity in Rab GTPase Domain Function / Maria Nussbaum ; Ruth N. Collins2：
Application of Phylogenetic Algorithms to Assess Rab Functional Relationships / 3：
Application of Protein Semisynthesis for the Construction of Functionalized Posttranslationally Modified Rab GTPases / Roger S. Goody ; Thomas Durek ; Herbert Waldmann ; Lucas Brunsveld ; Kirill Alexandrov4：
Assay and Functional Properties of PrBP(PDE[delta]), a Prenyl-Binding Protein Interacting with Multiple Partners / Houbin Zhang ; Suzanne Hosier ; Jennifer M. Terew ; Kai Zhang ; Rick H. Cote ; Wolfgang Baehr5：
Functional Assays for the Investigation of the Role of Rab GTPase Effectors in Dense Core Granule Release / Severine Cheviet ; Thierry Coppola ; Romano Regazzi6：
Analysis of Rab1 Recruitment to Vacuoles Containing Legionella pneumophila / Jonathan C. Kagan ; Takahiro Murata ; Craig R. Roy7：
Reconstitution of Rab4-Dependent Vesicle Formation In Vitro / Adriana Pagano ; Martin Spiess8：
Assay of Rab4-Dependent Trafficking on Microtubules / John W. Murray ; Allan W. Wolkoff9：
CD2AP, Rabip4, and Rabip4': Analysis of Interaction with Rab4a and Regulation of Endosomes Morphology / Pascale Monzo ; Muriel Mari ; Vincent Kaddai ; Teresa Gonzalez ; Yannick Le Marchand-Brustel ; Mireille Cormont10：
Visualization of Rab5 Activity in Living Cells Using FRET Microscopy / Emilia Galperin ; Alexander Sorkin11：
Selection and Application of Recombinant Antibodies as Sensors of Rab Protein Conformation / Clement Nizak ; Sandrine Moutel ; Bruno Goud ; Franck Perez12：
Analysis of Rab Protein Function in Neurotransmitter Receptor Trafficking at Hippocampal Synapses / Nashaat Z. Gerges ; Tyler C. Brown ; Susana S. Correia ; Jose A. Esteban13：
Use of Rab GTPases to Study Lipid Trafficking in Normal and Sphingolipid Storage Disease Fibroblasts / Amit Choudhury ; David L. Marks ; Richard E. Pagano14：
Assay of Rab13 in Regulating Epithelial Tight Junction Assembly / Anne-Marie Marzesco ; Ahmed Zahraoui15：
Tyrosine Phosphorylation of Rab Proteins / Jean H. Overmeyer ; William A. Maltese16：
Assay of Rab25 Function in Ovarian and Breast Cancers / Kwai Wa Cheng ; Yiling Lu ; Gordon B. Mills17：
Functional Analysis of Rab27a Effector Granuphilin in Insulin Exocytosis / Tetsuro Izumi ; Hiroshi Gomi ; Seiji Torii18：
Assays for Functional Properties of Rab34 in Macropinosome Formation / Peng Sun ; Takeshi Endo19：
Fluorescent Microscopy-Based Assays to Study the Role of Rab22a in Clathrin-Independent Endocytosis / Roberto Weigert ; Julie G. Donaldson20：
Purification and Properties of Rab3 GEP (DENN/MADD) / Toshiaki Sakisaka ; Yoshimi Takai21：
Biochemical Characterization of Alsin, a Rab5 and Rac1 Guanine Nucleotide Exchange Factor / Justin D. Topp ; Darren S. Carney ; Bruce F. Horazdovsky22：
Purification and Analysis of RIN Family-Novel Rab5 GEFs / Kota Saito ; Hiroaki Kajiho ; Yasuhiro Araki ; Hiroshi Kurosu ; Kenji Kontani ; Hiroshi Nishina ; Toshiaki Katada23：
Purification and Functional Properties of a Rab8-Specific GEF (Rabin3) in Action Remodeling and Polarized Transport / Katarina Hattula ; Johan Peranen24：
Assay and Functional Properties of SopE in the Recruitment of Rab5 on Salmonella-Containing Phagosomes / Seetharaman Parashuraman ; Amitabha Mukhopadhyay25：
Purification and Functional Analyses of ALS2 and its Homologue / Shinji Hadano ; Joh-E Ikeda26：
Polycistronic Expression and Purification of the ESCRT-II Endosomal Trafficking Complex / Aitor Hierro ; Jaewon Kim ; James H. Hurley27：
Analysis and Properties of the Yeast YIP1 Family of Ypt-Interacting Proteins / Catherine Z. Chen28：
Use of Hsp90 Inhibitors to Disrupt GDI-Dependent Rab Recycling / Christine Y. Chen29：
Purification and Properties of Yip3/PRA1 as a Rab GDI Displacement Factor / Ulf Sivars ; Dikran Aivazian ; Suzanne Pfeffer30：
Purification and Analysis of TIP47 Function in Rab9-Dependent Mannose 6-Phosphate Receptor Trafficking / Alondra Schweizer Burguete31：
Capture of the Small GTPase Rab5 by GDI: Regulation by p38 MAP Kinase / Michela Felberbaum-Corti ; Valeria Cavalli ; Jean Gruenberg32：
Rab2 Purification and Interaction with Protein Kinase C [iota]/[lambda] and Glyceraldehyde-3-Phosphate Dehydrogenase / Ellen J. Tisdale33：
Purification and Functional Interactions of GRASP55 with Rab2 / Francis A. Barr34：
Purification and Properties of Rabconnectin-3 / 35：
Physical and Functional Interaction of Noc2/Rab3 in Exocytosis / Tadao Shibasaki ; Susumu Seino36：
Functional Analysis of Slac2-a/Melanophilin as a Linker Protein between Rab27A and Myosin Va in Melanosome Transport / Taruho S. Kuroda ; Takashi Itoh ; Mitsunori Fukuda37：
Identification and Biochemical Analysis of Slac2-c/MyRIP as a Rab27A-, Myosin Va/VIIa-, and Actin-Binding Protein / 38：
Analysis of the Role of Rab27 Effector Slp4-a/Granuphilin-a in Dense-Core Vesicle Exocytosis / Eiko Kanno39：
Assay and Functional Interactions of Rim2 with Rab3 / 40：
Assay of the Rab-Binding Specificity of Rabphilin and Noc2: Target Molecules for Rab27 / Akitsugu Yamamoto41：
Functional Properties of the Rab-Binding Domain of Rab-Coupling Protein / Andrew J. Lindsay ; Nicolas Marie ; Mary W. McCaffrey42：
Purification and Functional Properties of Rab11-FIP2 / 43：
Purification and Functional Properties of Rab11-FIP3 / Conor P. Horgan ; Tomas H. Zurawski44：
Class I FIPs, Rab11-Binding Proteins That Regulate Endocytic Sorting and Recycling / Elizabeth Tarbutton ; Andrew A. Peden ; Jagath R. Junutula ; Rytis Prekeris45：
Expression and Properties of the Rab4, Rabaptin-5[alpha], AP-1 Complex in Endosomal Recycling / Ioana Popa ; Magda Deneka ; Peter van der Sluijs46：
Measurement of the Interaction of the p85[alpha] Subunit of Phosphatidylinositol 3-Kinase with Rab5 / M. Dean Chamberlain ; Deborah H. Anderson47：
Assay and Stimulation of the Rab5 GTPase by the p85[alpha] Subunit of Phosphatidylinositol 3-Kinase / 48：
Ubiquitin Regulation of the Rab5 Family GEF Vps9p / Brian A. Davies49：
Analysis of the Interaction between GGA1 GAT Domain and Rabaptin-5 / Guangyu Zhu ; Peng Zhai ; Nancy Wakeham ; Xiangyuan He ; Xuejun C. Zhang50：
Purification and Properties of Rab6 Interacting Proteins / Solange Monier51：
Affinity Purification of Ypt6 Effectors and Identification of TMF/ARA160 as a Rab6 Interactor / Symeon Siniossoglou52：
Assay and Properties of Rab6 Interaction with Dynein-Dynactin Complexes / Evelyn Fuchs ; Benjamin Short53：
Assay and Functional Properties of Rabkinesin-6/Rab6-KIFL/MKlp2 in Cytokinesis / Rudiger Neef ; Ulrike Gruneberg54：
Interaction and Functional Analyses of Human VPS34/p150 Phosphatidylinositol 3-Kinase Complex with Rab7 / Mary-Pat Stein ; Canhong Cao ; Mathewos Tessema ; Yan Feng ; Elsa Romero ; Angela Welford ; Angela Wandinger-Ness55：
Functional Analyses and Interaction of the XAPC7 Proteasome Subunit with Rab7 / Sanchita Mukherjee ; Jianbo Dong ; Carrie Heincelman ; Melanie Lenhart56：
Expression, Assay, and Functional Properties of RILP / Anna Maria Rosaria Colucci ; Maria Rita Spinosa ; Cecilia Bucci57：
Assay and Functional Properties of Rab34 Interaction with RILP in Lysosome Morphogenesis / Tuanlao Wang ; Wanjin Hong58：
Rabring7: A Target Protein for Rab7 Small G Protein / Kouichi Mizuno ; Ayuko Sakane ; Takuya Sasaki59：
Analysis of Potential Binding of the Recombinant Rab9 Effector p40 to Phosphoinositide-Enriched Synthetic Liposomes / Diego Sbrissa ; Ognian C. Ikonomov ; Assia Shisheva60：
Assessment of Rab11-FIP2 Interacting Proteins In Vitro / Nicole A. Ducharme ; Min Jin ; Lynne A. Lapierre ; James R. Goldenring61：
Interactions of Myosin Vb with Rab11 Family Members and Cargoes Traversing the Plasma Membrane Recycling System / 62：
Properties of Rab13 Interaction with Protein Kinase A / 63：
Functional Properties of Rab15 Effector Protein in Endocytic Recycling / Lisa A. Elferink ; David J. Strick64：
Assays for Interaction between Rab7 and Oxysterol Binding Protein Related Protein 1L (ORP1L) / Marie Johansson ; Vesa M. Olkkonen65：
Characterization of Rab23, a Negative Regulator of Sonic Hedgehog Signaling / Timothy M. Evans ; Fiona Simpson ; Robert G. Parton ; Carol Wicking66：
Purification and Functional Analysis of a Rab27 Effector Munc13-4 Using a Semiintact Platelet Dense-Granule Secretion Assay / Ryutaro Shirakawa ; Tomohito Higashi ; Hirokazu Kondo ; Akira Yoshioka ; Toru Kita ; Hisanori Horiuchi67：
Analysis of hVps34/hVps15 Interactions with Rab5 In Vivo and In Vitro / James T. Murray ; Jonathan M. Backer68：
Purification and Functional Properties of Prenylated Rab Acceptor 2 / Pierre-Yves Gougeon ; Johnny K. Ngsee69：
Author Index
Subject Index
Contributors to Volume 403
Preface
Volumes in Series
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