Contributors |
Volumes in Series |
Protein 3-Nitrotyrosine in Complex Biological Samples: Quantification by High-Pressure Liquid Chromatography/Electrochemical Detection and Emergence of Proteomic Approaches for Unbiased Identification of Modification Sites / Tal Nuriel ; Ruba S. Deeb ; David P. Hajjar ; Steven S. Gross1: |
Introduction |
Quantification of 3-NT in Proteins Using HPLC Separation and Electrochemical (EC) Detection / 2: |
Protocol for Quantification of 3-NT in Hydrolyzed Proteins Using HPLC-EC / 3: |
Beyond Quantification: Specification of 3-NT Sites in Proteins / 4: |
Acknowledgment |
References |
Selective Fluorogenic Derivatization of 3-Nitrotyrosine and 3,4-Dihydroxyphenylalanine in Peptides: A Method Designed for Quantitative Proteomic Analysis / Victor S. Sharov ; Elena S. Dremina ; Justin Pennington ; Jacque Killmer ; Christopher Asmus ; Maria Thorson ; Sung Jung Hong ; Xiaobao Li ; John F. Stobaugh ; Christian Schoneich |
Design of a Fluorogenic Derivatization Method |
Model Studies with 4-Methylcatechol and 2-Aminocresol |
Derivatization of a 3-Nitrotyrosine-Containing Model Peptide with Benzylamine and 4-Aminomethylbenzene Sulfonic Acid |
Conclusions / 5: |
Nitroalkenes: Synthesis, Characterization, and Effects on Macrophage Activation / Ana Maria Ferreira ; Andres Trostchansky ; Mariana Ferrari ; Jose M. Souza ; Homero Rubbo |
Synthesis and Characterization of Cholesteryl-Nitrolinoleate |
Synthesis and Identification of Nitroalkenes Isomers: Mass Spectrometry Analysis of Nitroarachidonate |
Determination of Lipid Nitration during Macrophage Activation |
Effects of Nitroalkenes on NOS2 Induction by Activated Macrophages |
Analysis of HO-1 Induction and NF-[kappa]B Activation / 6: |
Acknowledgments |
In-Gel Detection of S-Nitrosated Proteins Using Fluorescence Methods / Nicholas J. Kettenhofen ; Xunde Wang ; Mark T. Gladwin ; Neil Hogg |
Role of Trace Metal Ions in Ascorbate-Mediated Reduction of S-Nitrosothiols |
Detection of Protein S-Nitrosation Using Fluorescent Labeling Methods |
Current Protocol for CyDye-Switch Method |
Difference Gel Electrophoresis Analysis of a Model Protein Mixture |
Sensitivity of Detection of the CyDye Label |
Fluorescence Detection of S-Nitrosated Proteins in Plasma / 7: |
Two-Dimensional Difference Gel Electrophoresis Detection of S-Nitrosated Proteins / 8: |
Modifications of the Original Biotin-Switch Method / 9: |
Current Procedure for the Biotin-Switch Assay / 10: |
Conclusion / 11: |
The Arachidonate-Dependent Survival Signaling Preventing Toxicity in Monocytes/Macrophages Exposed to Peroxynitrite / Orazio Cantoni ; Ilaria Tommasini ; Liana Cerioni |
Materials and Methods |
Results and Discussion |
Practical Approaches to Investigate Redox Regulation of Heat Shock Protein Expression and Intracellular Glutathione Redox State / Vittorio Calabrese ; Anna Signorile ; Carolin Cornelius ; Cesare Mancuso ; Giovanni Scapagnini ; Bernardo Ventimiglia ; Nicolo' Ragusa ; Albena Dinkova-Kostova |
Nitric Oxide and Cellular Stress Response: Role of Vitagenes |
Material and Methods |
Results |
Discussion |
Monitoring Oxidative Stress in Vascular Endothelial Cells in Response to Fluid Shear Stress: From Biochemical Analyses to Micro- and Nanotechnologies / Mahsa Rouhanizadeh ; Wakako Takabe ; Lisong Ai ; Hongyu Yu ; Tzung Hsiai |
In Vitro Monitoring of Reactive Species |
Measurement of Endothelial ROS Generation in Response to Shear Stress |
Protein Oxidation and Nitration in Response to Shear Stress |
Emerging Technology: Micro- and Nanotechnology |
Summary |
Determination of S-Nitrosothiols in Biological and Clinical Samples Using Electron Paramagnetic Resonance Spectrometry with Spin Trapping / Paul G. Winyard ; Iona A. Knight ; Frances L. Shaw ; Sophie A. Rocks ; Claire A. Davies ; Paul Eggleton ; Richard Haigh ; Matthew Whiteman ; Nigel Benjamin |
Relative Yield of Spin-Trapped NO[superscript middle dot] from S-Nitrosoalbumin in Comparison with S-Nitrosoglutathione |
S-Nitrosoalbumin Recovery |
Potential Signal Contribution from N-Nitrosamines and Other Possible Sources of NO[superscript middle dot] |
Use of the EPR Assay for S-Nitrosothiols in Combination with Isotopic Labeling |
Novel Method for Measuring S-Nitrosothiols Using Hydrogen Sulfide / Xinjun Teng ; T. Scott Isbell ; Jack H. Crawford ; Charles A. Bosworth ; Gregory I. Giles ; Jeffrey R. Koenitzer ; Jack R. Lancaster ; Jeannette E. Doeller ; David W. Kraus ; Rakesh P. Patel |
Experimental Manipulation of H[subscript 2]S |
Specificity for H[subscript 2]S-Dependent Detection of RSNO |
Comparison of H[subscript 2]S vs Tri-iodide and Copper/Cysteine Based Methods for RSNO Detection |
Effects of pH on H[subscript 2]S-Dependent RSNO Detection |
Effect of Antifoam |
Summary and Conclusions |
Kinetic Studies on Peroxynitrite Reduction by Peroxiredoxins / Madia Trujillo ; Gerardo Ferrer-Sueta ; Rafael Radi |
General Considerations for Reagents Used |
Kinetics Studies of the Oxidative Part of the Catalytic Cycle |
Reductive Part of the Catalytic Cycle |
Nitrocytochrome c: Synthesis, Purification, and Functional Studies / Laura Castro ; Adriana Maria Cassina ; Carlos Batthyany |
Synthesis of Tyrosine-Nitrated Cytochrome c |
Purification of the Mononitrated Cytochrome c Species |
Mapping the Sites of Tyrosine Nitration and Spectral Characterization of the Nitrated Cytochrome c |
Functional Consequences of Nitrated Cytochrome c |
Concluding Remarks |
Tyrosine Nitration, Dimerization, and Hydroxylation by Peroxynitrite in Membranes as Studied by the Hydrophobic Probe N-t-BOC-l-tyrosine tert-Butyl Ester / Silvina Bartesaghi ; Gonzalo Peluffo ; Hao Zhang ; Joy Joseph ; Balaraman Kalyanaraman12: |
Methods |
Assessment of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation Products / Francisco R. M. Laurindo ; Denise C. Fernandes ; Celio X. C. Santos13: |
Probes for Assessment of Superoxide |
Dihydroethidium as a Superoxide Probe |
HPLC Analysis of Dihydroethidium Oxidation in Cells and Tissues: General Considerations |
Extraction of Fluorescent DHE-Derived Products in Biological Samples |
Quantification of Fluorescent DHE-Derived Products |
Assessment of NADPH Oxidase Activity in Cell Membrane Fraction by DHE Oxidation |
Methods to Measure the Reactivity of Peroxynitrite-Derived Oxidants Toward Reduced Fluoresceins and Rhodamines / Peter Wardman14: |
Reactants |
Sources of Error and Scope for Improvements in Methodology |
Detection and Characterization of Peroxynitrite-Induced Modifications of Tyrosine, Tryptophan, and Methionine Residues by Tandem Mass Spectrometry / Igor Rebrin ; Catherine Bregere ; Timothy K. Gallaher ; Rajindar S. Sohal15: |
Experimental Procedures |
Anticipated Results |
Reductive Gas-Phase Chemiluminescence and Flow Injection Analysis for Measurement of the Nitric Oxide Pool in Biological Matrices / Ulrike Hendgen-Cotta ; Marijke Grau ; Tienush Rassaf ; Putrika Gharini ; Malte Kelm ; Petra Kleinbongard16: |
Circulating and Resident Pool of Nitric Oxide |
Applied Methods for Determination of Nitrite, Nitrate, and Nitroso Species in Biological Matrices and Iron-Nitrosyls in Tissue |
Sample Processing |
Detection and Measurement for the Modification and Inactivation of Caspase by Nitrosative Stress In Vitro and In Vivo / Hee-Jun Na ; Hun-Taeg Chung ; Kwon-Soo Ha ; Hansoo Lee ; Young-Guen Kwon ; Timothy R. Billiar ; Young-Myeong Kim17: |
Nitrosative Stress |
Biological Reactivity of Nitric Oxide |
S-Nitrosylating Chemistry |
S-Nitrosylating Specificity |
S-Nitrosylation of Caspases |
Interactive Relations between Nitric Oxide (NO) and Carbon Monoxide (CO): Heme Oxygenase-1/CO Pathway Is a Key Modulator in NO-Mediated Antiapoptosis and Anti-inflammation / Byung-Min Choi18: |
Induction of HO-1 by the NOS/NO Pathway |
Antiapoptotic Roles of the NOS/HO-1 Pathway |
Anti-inflammatory Roles of the NOS/HO-1 Pathway |
Interactive Relations between NO and CO |
Detection and Characterization of In Vivo Nitration and Oxidation of Tryptophan Residues in Proteins / 19: |
In Vivo Real-Time Measurement of Nitric Oxide in Anesthetized Rat Brain / Rui M. Barbosa ; Catia F. Lourenco ; Ricardo M. Santos ; Francois Pomerleau ; Peter Huettl ; Greg A. Gerhardt ; Joao Laranjinha20: |
Experimental Considerations |
In Vivo Experiments: Results and Discussion |
Nitric Oxide and Cardiobiology-Methods for Intact Hearts and Isolated Myocytes / Joshua M. Hare ; Farideh Beigi ; Konstantinos Tziomalos21: |
Nitroso-Redox Signal Mediators |
Nitric Oxide and Signaling Through Cysteine Thiols |
Nitroso-Redox Balance or Imbalance |
Nitric Oxide Synthases, NADPH Oxidase, and Xanthine Oxidoreductase (XOR) |
Nitric Oxide-Redox Balance in Excitation-Contraction Coupling |
Techniques for Assessing Oxidative and Nitrosative Stress |
Techniques Used for Measuring S-Nitrosylation |
Activity Measurements |
Microscopic Technique for the Detection of Nitric Oxide-Dependent Angiogenesis in an Animal Model / Seung Namkoong ; Byoung-Hee Chung22: |
Summary and Conclusion |
Author Index |
Subject Index |
Contributors |
Volumes in Series |
Protein 3-Nitrotyrosine in Complex Biological Samples: Quantification by High-Pressure Liquid Chromatography/Electrochemical Detection and Emergence of Proteomic Approaches for Unbiased Identification of Modification Sites / Tal Nuriel ; Ruba S. Deeb ; David P. Hajjar ; Steven S. Gross1: |