Purification and Biochemical Properties of Rac1,2,3 and the Splice Variant Rac1b |
Biochemical Analyses of the Wrch Atypical Rho Family GTPases |
Purification of P-Rex1 from neutrophils and nucleotide exchange assay |
In vitro guanine nucleotide exchange activity of DHR-2/DOCKER/ CZH2 domains |
Biochemical characterization of the Cool (Coned-out-of-Library)/Pix (Pak-interactive exchange factor) proteins |
GEF and glucosylation assays on liposome bound Rac |
Phosphorylation of RhoGDI by p21-activated kinase 1 |
Purification of ARAP3 and characterization of GAP activities |
Regulation of RhoGAP specificity by phospholipids and prenylation |
Purification and activity of the Rho ADP-ribosylating binary C2/C3 toxin |
Purification of Tat-C3 exoenzymes |
Imaging and photobleach correction of MeroCBD, sensor of endogenous Cdc42 activation |
Cdc42 and PI(4,5)P2-induced actin assembly in Xenopus egg extracts |
In Vitro Reconstitution of Cdc42-Mediated Actin Assembly Using Purified Components |
Biochemical Analysis of Mammalian Formin Effects on Actin Dynamics |
Formin proteins: purification and measurement of effects on actin assembly |
Purification and Kinase Assay of PKN |
Purification and Enzyme Activity of ACK1 |
Direct activation of purified phospholipase C epsilon by RhoA studied in reconstituted phospholipid vesicles |
Regulation of PLCG isoforms by Rac |
Biochemical properties and inhibitors of (N-)WASP |
The Use of GFP to localize Rho GTPases in Living Cells |
Analysis of the Spatio-Temporal Activation of Rho GTPases using Raichu Probes |
Measurement of Activity of Rho GTPases during Mitosis |
RNAi inhibition of Rho GTPases |
RNA interference techniques to study epithelial cell adhesion and polarity |
Nucleofection of primary neurons |
Dock180-ELMO Cooperation in Rac Activation |
Rho GTPase activation by cell-cell adhesion |
Activation of Rap1, Cdc42, and Rac by nectin adhesion system |
Analysis of activated GAPs and GEFs in cell lysates |
Degradation of RhoA by Smurf1 ubiquitin ligase |
Ubiquitin-mediated proteasomal degradation of Rho proteins by the CNF1 toxin |
Regulation of superoxide-producing NADPH in non-phagocytic cells |
Activation of MEKK1 by Rho GTPases |
Activation of Apoptotic JNK Pathway through the Rac1-Binding Scaffold Protein POSH |
Quantification of isozyme-specific activation of phospholipase C-G2 by Rac GTPases and phospholipase C-s by Rho GTPases in an intact cell assay system |
Activation of Rho and Rac by Wnt/Frizzled signalling |
Fluorescent assay of cell permeable C3 transferase activity |
Use of TIRF microscopy to visualize actin and microtubules in migrating cells |
Inhibition of ROCK by RhoE |
Conditional regulation of a ROCK-estrogen receptor fusion protein |
Rational design and applications of a Rac GTPase-specific small molecule inhibitor |
In vitro assay of primary astrocyte migration as a tool to study Rho GTPase function in cell polarization |
Real time centrosome reorientation during fibroblast migration |
Lentiviral delivery of RNAi in Hippocampal Neurons |
Methods for studying neutrophil Chemtoaxis |
Measurement of Epidermal Growth Factor Receptor turnover and effects of Cdc42 |
Tumor cell migration in three dimensions |
Reciprocal regulation of cyclin D1 expression by Rac and Rho |
Regulation of ERK signal duration and cyclin D1 expression by Rhodependent stress fiber formation |
An in vitro model to study the role of endothelial Rho GTPases during leukocyte transendothelial migration |
Analysis of a Mitotic role of Cdc42 |
Plexin-induced collapse assay in COS cells |
Morphological and Biochemical Analysis of Rac1 in Three-Dimensional Epithelial Cell Cultures |
Using Three Dimensional Acinar Structures for Molecular and Cell Biological Assays |
TC10 and insulin-stimulated glucose transport |
GTPases and the control of neuronal polarity |
In vitro assembly of filopodia-like bundles |
Purification and Biochemical Properties of Rac1,2,3 and the Splice Variant Rac1b |
Biochemical Analyses of the Wrch Atypical Rho Family GTPases |
Purification of P-Rex1 from neutrophils and nucleotide exchange assay |