Preface |
List of Color Figures and Website Materials |
Introduction / John R. PilbrowChapter 1: |
Iron-Sulfur Cluster-Containing Proteins / 1: |
Molybdenum Enzymes / 2: |
Manganese-Containing Enzymes / 3: |
Novel Metalloenzymes and Metalloproteins / 4: |
Conclusions / 5: |
Iron-Sulfur-Containing Proteins |
Electron Magnetic Resonance of Iron-Sulfur Proteins in Electron-Transfer Chains: Resolving Complexity / Richard Cammack ; Froser MacMillanChapter 2: |
Problems of Complex Electron-Transfer Systems / 1.1: |
Iron-Sulfur Proteins |
Types of Clusters / 2.1: |
Information from Advanced EMR |
Relaxation Rates / 3.1: |
Identification of Cluster Ligands / 3.2: |
Interactions with Protons and Paramagnets / 3.3: |
Further Structural Information / 3.4: |
Orientation-Selective ENDOR and ESEEM / 3.5: |
Studies of Intact Membrane-Bound Complexes / 3.6: |
Methods of Isolating Spectra of Individual Components / 3.7: |
Results from 14N ESEEM / 3.8: |
Selected Examples from Electron-Transport Chains |
Xanthine Dehydrogenase/Oxidase as a Model / 4.1: |
Mitochondria and Aerobic Bacteria / 4.2: |
Complex I (NADH: Ubiquinone Reductase) / 4.3: |
Complex II (Succinate:Quinone Reductase) and Quinol: Fumarate Reductase / 4.4: |
Complex III / 4.5: |
Microbial Anaerobic Respiration / 4.6: |
Catalysis and Gene Regulation / Helmut BeinertChapter 3: |
Iron-Sulfur Clusters in "Radical SAM" Enzymes: Spectroscopy and Coordination / Serge Gambarelli1 ; Etienne Mulliez2 ; Marc FontecaveChapter 4: |
"Radical SAM" Iron-Sulfur Enzymes: An Example of a Low-Molecular-Weight Ligand to a [4Fe-4S] Cluster |
The Pyruvate Formate Lyase System |
Lysine 2,3-Aminoniutase / 2.2: |
Anaerobic Ribonucleotide Reductase / 2.3: |
Detection of Hyperfine Coupling Interactions in Metalloproteins |
ENDOR: Principles and General Considerations |
ESEEM and HYSCORE |
Analysis of Ligand Hyperfine Coupling Interactions |
Applications to Metalloproteins |
Pyruvate Formate Lyase-Activating Enzyme (PFL-AE) / 5.1: |
Lysine 2,3-Aminomutase (LAM / 5.2: |
Anaerobic Ribonucleotide Reductase Activating Enzyme (aRNR-AE) / 5.3: |
Conclusion / 6: |
Mononuclear Molybdenum Enzymes |
EPR Studies of Xanthine Oxidoreductase and Other Molybdenum-Containing Hydroxylases / Russ HilleChapter 5: |
Historical Context |
The Active Site Structure of Xanthine Oxidoreductase |
Isotopic Substitution Studies |
Magnetic Interactions Between Centers in Xanthine Oxidoreductase |
Concluding Comments |
High-Resolution EPR Spectroscopy of Mo Enzymes. Sulfite Oxidases: Structural and Functional Implications / John H. Enemark ; A. V. Astashkin ; A.M. RaitsimringChapter 6: |
Introduction and Structures from X-Ray Crystallography |
Earlier cw EPR Investigations |
Frequencies Observed in Pulsed EPR for a System of Electron Spin S = 1/2 and Arbitrary Nuclear Spin in Weak Interaction Limit |
Pulsed EPR Techniques Used in This Work |
ENDOR |
ESEEM Techniques |
General Problems in Extraction of Structural Parameters from Magnetic Resonance Parameters |
Sample Preparation and Instrumentation |
High-Resolution Pulsed EPR Spectra, Magnetic Resonance Parameters, and Structural Implications for Various Forms of SO / 7: |
Exchangeable Protons: Similarities and Differences in SOs from Different Organisms / 7.1: |
Groups Blocking Water Access to Mo(V) / 7.2: |
Nonexchangeable Protons / 7.3: |
Exchangeable Oxygen Ligands / 7.4: |
Biological Implications / 8: |
Note Added in Proof / 9: |
Dimethylsulfoxide (DMSO) Reductase, a Member of the DMSO Reductase Family of Molybdenum Enzymes / Graeme R. Hanson ; Ian LaneChapter 7: |
EPR Studies of Mo(V) Species |
EPR. Studies of DMSO Reductase |
Respiratory DMSO Reductase |
Peripiasmic DMSO Reductase |
Catalytic Mechanism |
The Manganese-Calcium Cluster of the Oxygen-Evolving System: Synthetic Models, EPR Studies, and Electronic Structure Calculations / Marcin Brynda ; R. David BrittChapter 8: |
Theoretical Background for the Polynuclear Manganese Clusters |
Introduction to the Spin Physics of Exchange-Coupled Manganese Complexes |
EPR Theory for Exchange Coupled Systems |
Computational Methods for Magnetically Coupled Homonuclear Metal Clusters |
Synthetic Models for Manganese Cluster of the OEC |
Current Structural Proposals for the Pentanuclear Mn4Ca Cluster of the OEC |
EPR Characteristics of the Manganese Cluster of the OEC |
Synthetic Models |
Computational Studies of the OEC |
Calculations on the Mechanistic Aspects of the Water Oxidation with DFT |
Mixed Molecular Mechanics/Quantum Mechanics Studies of Water Oxidation |
Conclusions and Prospectives |
Appendix |
Manganese Metalloproteins / Sarah J. Smith ; Kieran S. Hadler ; Gerhard Schenk ; NataÜa MitićChapter 9: |
Manganese Catalases |
Biochemical and Structural Characterization |
Spectroscopic Characterization |
Mechanistic Implications |
Ribonucleotide Reductase |
Biochemical and Structural Characierization |
Class Ib Ribonucleotide Reductases |
Manganese-Iron Oxygenases |
SoxB |
Bacteriophage $ Protein Phosphatase / 6.1: |
Purple Acid Phosphatase |
Phosphodiesterase / 8.1: |
Arginase / 9.1: |
Methionyl Aminopeptidase / 10.1: |
EPR of Cobalt-Substituted Zinc Enzymes / Brian Bennett11.1: |
Review of Cobalt-Substituted Enzymes |
Methods of Co(II) Insertion |
EPR Experimental Techniques and Considerations |
Spectral Interpretation |
Spectral Interpretation: A Case Study |
Complementary Techniques |
Hyperfine and Quadrupolar Interactions in Vanadyl Proteins and Model Complexes: Theory and Experiment / Sarah C. Larsen ; N. Dennis ChasteenChapter 11: |
Coordination Chemistry of VO2+ |
EPR Properties / 1.2: |
The Additivity Relationship for Predicting Ligand Environments / 1.3: |
The Ground State and Ligand Hyperfine Couplings / 1.4: |
ENDOR and ESEEM of Vanadyl Model Complexes |
14N Hyperfine and Quadrupole Coupling Constants |
1H and l7O Coupling Constants |
31P Hyperfine Coupling Constants |
51V Nuclear Quadrupole Coupling Constants / 2.4: |
Density Functional Theory Calculations of EPR Parameters in Vanadyl Model Complexes |
Overview of DFT Methods for Calculations of EPR Parameters |
DFT Calculations of Vanadium EPR Parameters |
DFT Calculations of Ligand Hyperfine and Quadrupole Coupling Constants |
Outlook |
Select Protein Studies |
Pyruvate Kinase |
S-Adenosylmethionine Synthetase |
Imidazole Glycerol Phosphate Dehydratase |
ATP Synthase |
D-Xylose Isomerase |
Transferrins |
Feritin / 4.7: |
Tissues |
Kidney and Liver |
Bone |
Index |
Preface |
List of Color Figures and Website Materials |
Introduction / John R. PilbrowChapter 1: |