Contributors |
Preface |
Volumes in Series |
Chemokines and Receptors in Disease / Section I: |
Chemokines in Human Breast Tumor Cells: Modifying Their Expression Levels and Determining Their Effects on the Malignancy Phenotype / Gali Soria ; Tsipi Meshel ; Adit Ben-Baruch1: |
Introduction |
Modifying Chemokine Expression in Breast Tumor Cells / 2: |
Establishment of Primary Local Breast Tumors and Pulmonary Metastases / 3: |
Acknowledgments |
References |
CCR5 Pharmacology Methodologies and Associated Applications / Roy Mansfield ; Sarah Able ; Paul Griffin ; Becky Irvine ; Ian James ; Malcolm Marcartney ; Ken Miller ; James Mills ; Carolyn Napier ; Iva Navratilova ; Manos Perros ; Graham Rickett ; Harriet Root ; Elna van der Ryst ; Mike Westby ; Patrick Dorr |
CCR5 Signaling Assays and Application to Quantify and Characterize Ligand-Dependent Agonism, Antagonism, and Inverse Agonism |
CCR5-Associated Ligand-Binding Assays |
Surrogate In Vitro Antiviral Assays / 4: |
CCR5 Site-Directed Mutagenesis and Ligand Docking Studies / 5: |
Non-HIV Indications-Associated Studies, Human CCR5 Knock-In Mice / 6: |
CXCR4 and Mobilization of Hematopoietic Precursors / Michael P. Rettig ; Pablo Ramirez ; Bruno Nervi ; John F. DiPersio |
HSPC Mobilizing Agents that Target the CXCL12/CXCR4 Axis |
Donor Selection for HSPC Mobilization |
Flow Cytometric Enumeration of Mobilized HSPCs |
Dosing and Kinetics of HSPC Mobilization by G-CSF and Plerixafor |
Functional Characterization of Mobilized HSPCs / 7: |
Concluding Remarks / 8: |
Acknowledgment |
Double-Label Nonradioactive In Situ Hybridization for the Analysis of Chemokine Receptor Expression in the Central Nervous System / Meizhang Li ; Richard M. Ransohoff |
Basic Protocol for ISH (Using Digoxygenin-Labeled Probe) |
Comments |
Expression of Chemokines and Chemokine Receptors in Human Colon Cancer / Marco Erreni ; Paolo Bianchi ; Luigi Laghi ; Massimiliano Mirolo ; Marco Fabbri ; Massimo Locati ; Alberto Mantovani ; Paola Allavena |
Materials and Methods |
Results |
Discussion |
Chemokine Related Proteins from Pathogens / Section II: |
Kaposi's Sarcoma Virally Encoded, G-Protein-Coupled Receptor: A Paradigm for Paracrine Transformation / Daniel Martin ; J. Silvio Gutkind |
Cloning of vGPCR |
Assaying vGPCR Transforming Activity In Vitro |
vGPCR Transforming Activity In Vivo Using Xenograft Systems |
In Vivo Targeted Infection Using the TVA-RCAS System |
vGPCR-Induced Paracrine Transformation |
Characterization of vGPCR-Induced Molecular Signaling |
Evaluation of Activation of Second-Messenger-Generating Systems |
Activation of Signal-Transducing Protein Kinases and Small GTPases / 9: |
Akt Kinase Assay / 10: |
vGPCR Stimulated Activation of Rac1-Pulldown Assays / 11: |
Western Blotting Using Phospho-Specific Antibodies / 12: |
Activation of Transcription Factors / 13: |
Global Changes in Gene Expression: Microarray Analysis / 14: |
NFkB Luciferase Assays / 15: |
NFkB Binding Assays / 16: |
Nuclear Translocation of NFkB / 17: |
Pharmacological and Biochemical Characterization of Human Cytomegalovirus-Encoded G Protein-Coupled Receptors / David Maussang ; Henry F. Vischer ; Andreas Schreiber ; Detlef Michel ; Martine J. Smit |
Virally Encoded GPCR Engineering |
vGPCR Expression, Trafficking, and Radioligand Binding |
vGPCR-Induced Signal Transduction |
vGPCR-Induced Oncogenesis |
Generation of Recombinant HCMV Strains by Markerless Bacterial Artificial Chromosome Mutagenesis |
Conclusions |
Identification and Characterization of Virus-Encoded Chemokine Binding Proteins / Antonio Alcami ; Abel Viejo-Borbolla |
Methods for Studying Chemokine-Binding Proteins |
The Chemokine-Binding Protein M3 as a Tool to Understand the Chemokine Network In Vivo / Sergio A. Lira ; Limin Shang ; Andrea P. Martin |
Generation of Transgenic Mice Expressing M3 in Insulin-Producing b Cells |
M3 Expression in Islets of Langerhans Blocks CCL2-, CCL21-, and CXCL13-Induced Migration of Cells to Islets |
M3 Expression in b Cells Blocks Cellular Infiltration and Prevents Diabetes Development |
Generation of a Conditional Transgenic System for Expression of M3 |
M-T7: Measuring Chemokine-Modulating Activity / Mee Y. Bartee ; Erbin Dai ; Liying Liu ; Ganesh Munuswamy-Ramanujam ; Colin Macaulay ; Dana Mclvor ; Grant McFadden ; Alexandra R. Lucas |
Protein Expression |
Quantifying the Effects of M-T7 In Vitro and Ex Vivo |
Quantifying the Effects of M-T7 on Vascular Inflammatory Responses in Rodent Vascular Transplant Models |
Preclinical Toxicity Testing |
Atypical and Novel Chemoattractants and Receptors / Section III: |
Role of the Chemokine Scavenger Receptor D6 in Balancing Inflammation and Immune Activation / Elena M. Borroni ; Chiara Buracchi ; Benedetta Savino ; Fabio Pasqualini ; Remo C. Russo ; Manuela Nebuloni ; Raffaella Bonecchi |
Methods |
Structure-Function Dissection of D6, an Atypical Scavenger Receptor / Robert J. B. Nibbs ; Pauline McLean ; Clare McCulloch ; Alan Riboldi-Tunnicliffe ; Emma Blair ; Yanshi Zhu ; Neil Isaacs ; Gerard J. Graham |
Modeling Small Molecule-Compound Binding to G-Protein-Coupled Receptors / Nagarajan Vaidehi ; James E. Pease ; Richard Horuk |
Similarity and Differences in the Crystal Structures of Class-A GPCRs Solved to Date |
GPCR Modeling Methods |
Computational Methods for Receptor Flexibility and Ligand-Induced Conformational Changes in GPCRs |
Validation of GPCR-Ligand Models |
Elucidation of Chemerin and Chemokine-Like Receptor-1 Function in Adipocytes by Adenoviral-Mediated shRNA Knockdown of Gene Expression / Kerry B. Goralski ; Christopher J. Sinal |
The 3T3-L1 Cell Model for Adipogenesis and Adipocyte Metabolism |
RNA Interference |
Methods for Adenoviral shRNA Knockdown of Chemerin and CMKLR1 in 3T3-L1 Cells |
Chemokine Signaling / Section IV: |
Characterization of Chemokine Receptor CXCR2 Interacting Proteins Using a Proteomics Approach to Define the CXCR2 "Chemosynapse" / Dayanidhi Raman ; Nicole F. Neel ; Jiqing Sai ; Raymond L. Mernaugh ; Amy-Joan L. Ham ; Ann J. Richmond |
Validation of the Interaction of Novel Proteins with CXCR2 |
Mutational Analysis of Residues at Interactive Interface of CXCR2 and CXCR2-Binding Proteins |
Radioactive Phosphorylation of CXCR2 Interacting Proteins |
Chemotaxis Assay |
Phosphoproteomic Analysis of Chemokine Signaling Networks / Morgan O'Hayre ; Catherina L. Salanga ; Pieter C. Dorrestein ; Tracy M. Handel |
Summary |
Monitoring NF-kB Mediated Chemokine Transcription in Tumorigenesis / Jinming Yang |
Development of NF-kB Reporter Model for Tumors |
Bioluminescent Imaging of Intratumor Signaling of Anesthetized Mice |
Cell-Based Assays for Kinase and Transcriptional Activity In Vitro |
Peripheral Spying of Intratumoral Signaling of Conscious Mice |
Analysis of Chemokine Receptor Endocytosis and Intracellular Trafficking / Tom Kershaw ; Silene T. Wavre-Shapton ; Nathalie Signoret ; Mark Marsh18: |
Receptor Detection |
Cells |
Monitoring Receptor Endocytosis |
Monitoring Receptor Recycling |
Monitoring Receptor Degradation |
Electron Microscopy Analysis of Receptor Internalization |
Measuring the Proximity of T-Lymphocyte CXCR4 and TCR by Fluorescence Resonance Energy Transfer (FRET) / Ashok Kumar ; Kimberly N. Kremer ; Olivia L. Sims ; Karen E. Hedin19: |
Assaying CXCR4-TCR Proximity via the PE/APC mAb FRET Approach |
Assaying CXCR4-TCR Proximity via the CFP/YFP Fusion Protein Approach |
Expression of CXCR4, a G-Protein-Coupled Receptor for CXCL12 in Yeast: Identification of New-Generation Inverse Agonists / Barry J. Evans ; Zixuan Wang ; James R. Broach ; Shinya Oishi ; Nobutaka Fujii ; Stephen C. Peiper20: |
Methods and Discussion |
Ubiquitination of Chemokine Receptors / Adriano Marchese21: |
Cell Culture and Transfections |
Agonist Treatment and Ubiquitination Assay |
E3 Ubiquitin Ligase AIP4 Mediates Ubiquitination of CXCR4 |
Author Index |
Subject Index |