| Introduction to Gene Manipulation |
| Why do we clone genes? |
| How do we clone genes?- the basic technique |
| Cutting and joining DNA Cloning Genes in E. coli Plasmids as vectors |
| Phage and cosmid vectors |
| Cloning strategies, gene libraries and cDNA cloning Recombinant selection and screening Expression of cloned genes |
| Analyzing DNA nucleotide sequences |
| Changing genes - macro- and micro-modifications |
| The polymerase chain reaction - an all embracing technique Impact of recombinant DNA on human genetics |
| Cloning in Organisms other than E. coli |
| Cloning in bacteria other than E. coli |
| Cloning in yeast and other microbial eukaryotes |
| Gene transfer to plants |
| Introducing genes into animal cells Transferring genes into animal oocytes, eggs and embryos Biotechnology: The generation of novelty |
| Introduction to Gene Manipulation |
| Why do we clone genes? |
| How do we clone genes?- the basic technique |
図書
