| Contributors |
| Preface |
| Local Probe Techniques / J. K. Heinrich Horber1.： |
| Introduction / I.： |
| Scanning Tunneling Microscopy / II.： |
| Atomic Force Microscopy / III.： |
| Force Spectroscopy / IV.： |
| Photonic Force Microscopy / V.： |
| References |
| The Atomic Force Microscope in the Study of Membrane Fusion and Exocytosis / Bhanu P. Jena ; Sang-Joon Cho2.： |
| Methods |
| AFM Studies on Live Cells |
| Identification of New Plasma Membrane Structures Involved in Exocytosis |
| Future of AFM in the Study of Live Cells |
| Atomic Force Microscope Imaging of Cells and Membranes / Eric Lesniewska ; Pierre Emmanuel Milhiet ; Marie-Cecile Giocondi ; Christian Le Grimellec3.： |
| AFM Equipment |
| AFM Operating Modes |
| Requirements for the Imaging of Intact Cells |
| Imaging of Cells |
| Imaging of Isolated Membranes / VI.： |
| Conclusion and Perspectives / VII.： |
| Measuring the Elastic Properties of Living Cells by the Atomic Force Microscope / Manfred Radmacher4.： |
| Principles of Measurement |
| Application to Cells |
| Mechanics of Cellular Dynamics |
| Summary |
| Cell Adhesion Measured by Force Spectroscopy on Living Cells / Martin Benoit5.： |
| Instrumentation |
| Preparations of the Force Sensor for Measurements with Living Cells |
| Cell Culture |
| Final Remarks |
| Molecular Recognition Studies Using the Atomic Force Microscope / Peter Hinterdorfer6.： |
| Experimental Approach |
| Dynamic Force Spectroscopy |
| Recognition Imaging |
| The Biophysics of Sensory Cells of the Inner Ear Examined by Atomic Force Microscopy and Patch Clamp / Matthias G. Langer ; Assen Koitschev7.： |
| Morphology and Function of Cochlear Hair Cells |
| AFM Technology |
| Applications |
| Discussion |
| Outlook |
| Biotechnological Applications of Atomic Force Microscopy / Guillaume Charras ; Petri Lehenkari ; Mike Horton8.： |
| Analysis |
| Application Examples |
| Future Directions and Improvements |
| Cellular Membranes Studied by Photonic Force Microscopy / Arnd Pralle ; Ernst-Ludwig Florin9.： |
| Experimental Considerations |
| Methods for Biological Probe Microscopy in Aqueous Fluids / Johannes H. Kindt ; John C. Sitko ; Lia I. Pietrasanta ; Emin Oroudjev ; Nathan Becker ; Mario B. Viani ; Helen G. Hansma10.： |
| Substrates/Surfaces |
| Basic Methods for Atomic Force Microscopy in Aqueous Fluids |
| Molecular Force Probing |
| Advanced Fluid Handling |
| Conclusion |
| Supported Lipid Bilayers as Effective Substrates for Atomic Force Microscopy / Daniel M. Czajkowsky ; Zhifeng Shao11.： |
| Preparation of the Supported Bilayer Substrates |
| Examples of Applications |
| Cryo-Atomic Force Microscopy / Sitong Sheng12.： |
| Designs and Instrumentation |
| Applications in Structural Biology |
| Deep Etching as the Preferred Sample Preparation Method |
| New Directions |
| Conformations, Flexibility, and Interactions Observed on Individual Membrane Proteins by Atomic Force Microscopy / Daniel J. Muller ; Andreas Engel13.： |
| High-Resolution AFM Imaging |
| Identification of Membrane Proteins |
| Observing the Oligomerization of Membrane Proteins |
| Unraveling the Conformational Variability of Membrane Proteins |
| Comparing AFM Topographs to Atomic Models |
| Conformational Changes of Native Membrane Proteins |
| Observing the Assembly of Membrane Proteins / VIII.： |
| Detecting Intra- and Intermolecular Forces of Proteins / IX.： |
| Conclusions and Perspectives / X.： |
| Single-Molecule Force Measurements / Aileen Chen ; Vincent T. Moy14.： |
| Experimental Design |
| Forced Unfolding of Single Proteins / S. M. Altmann ; P.-F. Lenne15.： |
| The Biological System |
| Forced Unfolding |
| Models |
| Conclusions and Prospects |
| Appendices |
| Developments in Dynamic Force Microscopy and Spectroscopy / A. D. L. Humphris ; M. J. Miles16.： |
| Active Q Control |
| Application of Active Q-Control AFM |
| Transverse Dynamic Force Techniques |
| Conclusions |
| Scanning Force Microscopy Studies on the Structure and Dynamics of Single DNA Molecules / Giampaolo Zuccheri ; Bruno Samori17.： |
| The Control of Adsorption of DNA on Surfaces |
| Air Imaging of DNA: Which Present, Which Future? |
| Imaging DNA in Fluid |
| DNA Manipulation with the SFM: The Controlled Dissection of DNA |
| The Study of DNA Conformations and Mechanics: Curvature and Flexibility |
| An Interesting Issue: The Shape of Supercoiled DNA |
| Index |
| Volumes in Series |
| Contributors |
| Preface |
| Local Probe Techniques / J. K. Heinrich Horber1.： |
図書
