John N. Abelson, Sidney P. Colowick, Yusuf A. Hannun, Alfred H. Merrill, Alfred Harrison Merrill, Jr., Alfred H. Merrill, Melvin I. Simon, A. Hannum Yusuf
Assays for the Synthesis of Sphingomyelin (Ceramide Phosphocholine) and Ceramide Phosphoethanolamine / J.A. Shayman ; A. Abe
Glucosylceramide Synthase: Assay and Properties / P. Marks ; Y. Kamisaka ; R.E. Pagano
Methods for Studying Glucosylceramide Synthase / H. Sprong ; G. van Meer ; P. van der Sluijs
UDP-Galactose: Ceramide Galactosyltransferase / S. Chatterjee
Assay of Lactosylceramide Synthase and Comments on Its Potential Role in Signal Transduction / G. Pohlentz ; C. Kaes ; K. Sandhoff
In Vitro Assays for Enzymes of Ganglioside Synthesis / F.B. Jungalwala ; P. Chatturvedi ; D.S. Newburg ; M.R. Natowicz
Analyses of Sulfatides and Enzymes of Sulfatide Metabolism
1-O-Acyleceramide Synthase / T-C. Lee
N-Acetylation of Shingosine by Platelet-Activating Factor: Sphingosine Transacetylase / A.S. Fischl ; Y. Liu ; A. Browdy ; A.E. Cremesti
Inositolphosphoryl Ceramide Synthase from Yeast / D.V. Lynch
Enzymes of Shingolipid Metabolism in Plants
Turnover / S. Lansmann ; O. Bartelsen
Purification and Characterization of Recombinant Human Acid Sphingomyelinase / B. Liu ; Y.A. Hannun
Purification of Rat Brain Membrane Neutral Sphingomyelinase / B.Liu
Sphingolmyelinase Assay using Radiolabeled Substrates / A.G. Barbone ; A.C. Jackson ; D.M. Ritchie ; D.C. Argentieri
Robotic Assay of Sphingomyelinase Activity for High-Throughput Screening / D.F. Hassler ; R. M. Laethem ; G.K. Smith
A High Through-put Sphingomyelinase Assay / R.T. Dobrowsky ; V.G. Rao
Analysis of Sphingomyelin Hydrolosis in Caveolar Membranes / M.N. Nikolova-Karakashian
Ceramidases / T.Linke ; S. Lansman
Purification of Acid Ceramidase from Human Placenta / S. Bajjalieh ; R. Batchelor
Ceramide Kinase / A.Olivera ; K.D. Barlow ; S. Spiegel
Assaying Sphingosine Kinase Activity / L.M. Obeid
Sphingosine 1-Phosphate Phosphatase / D.N. Brindley ; J. Xu ; R. Jasinska ; D.W. Waggoner
Analysis of Ceramide 1-Phosphate and Sphingosine 1-Phosphate Phosphatase Activities / P. Van Veldhover
Sphingosine 1-Phosphate Lyase / W. Bierfreund ; T. Kolter
Sphingolipid Hydrolases and Activator Proteins / R-D. Duan ; A. Nilsson
Sphingolipid Hydrolyzing Enzymes in the Gastrointestinal Tract / M. Basu ; P. Kelly ; M. Girzadas ; Z. Li ; S. Basu
Properties of Animal Ceramide Glycanases / M. Ito ; K. Kita ; T. Kurita ; N. Sueyoshi ; H. Izu
Enzymatic N-Deacylation of Shingolipids
Genetic Approaches / S. Ichikawa ; Y. Hirabayashi
Genetic Approaches for Studies of Glycolipid Synthetic Enzymes / N.Chung
Use of Yeast as a Model System for Studies of Sphingolipid Metabolism and Signaling
Inhibitors and Sphingolipid Biosynthesis / S.M. Mandala ; G. H. Harris
Isolation and Characterization of Novel Inhibitors of Sphingolipid Synthesis: Austalifungin, Viridiofungins, Rustmicin, and Khafrefungin / R.T. Riley ; R.D. Plattner
Fermentation, Partial Purification, and Use of Serine Palmitoyltransferase Inhibitors from Isaria (Cordyceps) sinclairii / F. Meredith
Isolation and Characterization of Fumonisins / L. Lee ; L. Shu
Assays for the Synthesis of Sphingomyelin (Ceramide Phosphocholine) and Ceramide Phosphoethanolamine / J.A. Shayman ; A. Abe
Glucosylceramide Synthase: Assay and Properties / P. Marks ; Y. Kamisaka ; R.E. Pagano
Methods for Studying Glucosylceramide Synthase / H. Sprong ; G. van Meer ; P. van der Sluijs
UDP-Galactose: Ceramide Galactosyltransferase / S. Chatterjee
Assay of Lactosylceramide Synthase and Comments on Its Potential Role in Signal Transduction / G. Pohlentz ; C. Kaes ; K. Sandhoff
In Vitro Assays for Enzymes of Ganglioside Synthesis / F.B. Jungalwala ; P. Chatturvedi ; D.S. Newburg ; M.R. Natowicz
Analyses of Sulfatides and Enzymes of Sulfatide Metabolism
1-O-Acyleceramide Synthase / T-C. Lee
N-Acetylation of Shingosine by Platelet-Activating Factor: Sphingosine Transacetylase / A.S. Fischl ; Y. Liu ; A. Browdy ; A.E. Cremesti
Inositolphosphoryl Ceramide Synthase from Yeast / D.V. Lynch
Enzymes of Shingolipid Metabolism in Plants
Turnover / S. Lansmann ; O. Bartelsen
Purification and Characterization of Recombinant Human Acid Sphingomyelinase / B. Liu ; Y.A. Hannun
Purification of Rat Brain Membrane Neutral Sphingomyelinase / B.Liu
Sphingolmyelinase Assay using Radiolabeled Substrates / A.G. Barbone ; A.C. Jackson ; D.M. Ritchie ; D.C. Argentieri
Robotic Assay of Sphingomyelinase Activity for High-Throughput Screening / D.F. Hassler ; R. M. Laethem ; G.K. Smith
A High Through-put Sphingomyelinase Assay / R.T. Dobrowsky ; V.G. Rao
Analysis of Sphingomyelin Hydrolosis in Caveolar Membranes / M.N. Nikolova-Karakashian
Ceramidases / T.Linke ; S. Lansman
Purification of Acid Ceramidase from Human Placenta / S. Bajjalieh ; R. Batchelor
Ceramide Kinase / A.Olivera ; K.D. Barlow ; S. Spiegel
Assaying Sphingosine Kinase Activity / L.M. Obeid
Sphingosine 1-Phosphate Phosphatase / D.N. Brindley ; J. Xu ; R. Jasinska ; D.W. Waggoner
Analysis of Ceramide 1-Phosphate and Sphingosine 1-Phosphate Phosphatase Activities / P. Van Veldhover
Sphingosine 1-Phosphate Lyase / W. Bierfreund ; T. Kolter
Sphingolipid Hydrolases and Activator Proteins / R-D. Duan ; A. Nilsson
Sphingolipid Hydrolyzing Enzymes in the Gastrointestinal Tract / M. Basu ; P. Kelly ; M. Girzadas ; Z. Li ; S. Basu
Properties of Animal Ceramide Glycanases / M. Ito ; K. Kita ; T. Kurita ; N. Sueyoshi ; H. Izu
Enzymatic N-Deacylation of Shingolipids
Genetic Approaches / S. Ichikawa ; Y. Hirabayashi
Genetic Approaches for Studies of Glycolipid Synthetic Enzymes / N.Chung
Use of Yeast as a Model System for Studies of Sphingolipid Metabolism and Signaling
Inhibitors and Sphingolipid Biosynthesis / S.M. Mandala ; G. H. Harris
Isolation and Characterization of Novel Inhibitors of Sphingolipid Synthesis: Austalifungin, Viridiofungins, Rustmicin, and Khafrefungin / R.T. Riley ; R.D. Plattner
Fermentation, Partial Purification, and Use of Serine Palmitoyltransferase Inhibitors from Isaria (Cordyceps) sinclairii / F. Meredith
Isolation and Characterization of Fumonisins / L. Lee ; L. Shu
Antisense Receptor Targets / J. Garz=n ; I. de Antonio ; P. Sfnchez-Blfzquez
In vivo Modulation of G-proteins and Opioid Receptor Function by Antisense Oligodeoxynucleotides / J. Karle ; M. Nielsen
Targeting Brain GABA-A Receptors with Antisense Oligonucleotides: Implications for Epilepsy / D. Mohuezy ; X. Tang ; M.I. Phillips
Delivery of Antisense DNA by Vectors for Prolonged Effects in Vitro and in Vivo / G.W. Pasternak ; Y.-X. Pan
Antisense Mappling: Assessing the Functional Significance of Genes and Splice Variants / E.S.J. Robinson ; A.L. Hudson
In Vivo and in Vitro Effects of Antisense on 2-Adrenoceptor Expression / J.G. Hensler ; J.M. Scalzitti
Design and Efficacy of a Seritonin2A Receptor Antisense Oligodeoxynucleotide / M. Beinfeld ; D. Vishnuvardham
The Use of Expression of Antisense mRNA for the Enzymes PC1 and PC2 in Prohormone Processing
Antisense Neuroscience Targets / W.C. Broaddus ; S.S. Prabhu ; S. Wu-Pong ; G.T. Gillies ; H. Fillmore
Design and Testing of Antisense Oligonucleotide Strategies for the Central Nervous System / N.J. Buckley ; Fe.C. Abogadie ; D.A. Brown ; M. Dayrell ; M.P. Caulfield ; P. Delmas ; J.E. Haley
Use of Antisense Expression Plasmids to Attenuate G-Expression in Primary Neurons / S. Catsicas ; F. Hallb__k ; M. Catsicas ; J.K. Staple ; G. Grenningloh
Gene Functional Analysis in the Nervous System / S. Peng Ho ; D.H.O. Britton ; Y. Bao ; and M.S. Scully
RNA Mapping: Selection of Potent Oligonucleotide Sequences for the Antisense Experiment / P.S. Kalra ; M.G. Dube ; S.P. Kalra
Effects of Centrality Administered Antisense Oligodeoxynucleotides on Feeding Behavior and Hormone Secretion / J. Lai ; J.C. Hunter ; M.H. Ossipov ; F. Porreca
Blockade of Neuropathic Pain by Antisense Targeting of TTX-Resistant Sodium Channels in Sensory Neurons / R. Mileusnic
Application of Antisense Approach for the Study of Cell Adhesion Molecules in the Central Nervous System / I.D. Neumann ; N. Toschi
Sequence Design and Practical Implementation of Antisense Oligonucleotides in Neuroendocrinology / T.S. Nowak, Jr. ; K. Yufu ; Y. Yaida
Localization of Oligonucleotides in Brain by in situ Hybridization / F. Gremo ; V. Sogos ; M. Curot ; M. Setzu ; I. Mussini ; M. Grazia Ennas
Use of Antisense Oligonucleotides in Human Neuronal and Astrocytic Cultures / W. Sommer ; M.O. Hebb ; M. Heilig
Pharmacokinetic Properties of Oligonucleotides in the Brain / W. Tischmeyer
Antisense Oligonucleotides--Preparation and in vivo Application to Rat Brain / S.G. Volsen ; R.C. Lambert ; Y. Maulet ; M.D. Waard ; S. Gillard ; P.J. Craig ; A. Feltz
The Application of Antisense Techniques to Characterize NEuronal Ion Channels in vitro
Antisense in Non-Neuronal Tissues / M. Juhaszova ; M.K. Slodzinski
Antisense Inhibition of the Na-Ca Exchanger / E.A.L. Biessen ; H. Vietsch ; E.T. Rump ; K. Fluiter ; M.K. Bijsterbosch ; Th.J.C. van Berkel
Targeted Delivery of Antisense Oligonucleotides to Parenchymal Liver Cell in vivo / D. Mercola ; F. Bost ; R. McKay ; N.M. Dean
Antisense Methods for the Discrimination of Phenotypic Properties of Closely Related Gene Products: The Jun Kinase Family / C.L. Cioffi ; B.P. Monia
Evaluation of the Biological Role of c-Jun N-terminal Kinase (NJK) using an Antisense Approach / M.B. Ganz
The Role of Antisense in Kidney Cells / D.L. Mattson
Use of Antisense Techniques in the Rate Renal Medulla / T. Ochiya ; M. Terada
Antisense Approaches to in vitro Organ Culture / E.S. Raveche ; G.A. Parker ; B. Peng ; M. He ; S. Gould-Fogerite ; C.-C. Chou
In vitro and in vivo Anti-Proliferative
Antisense Receptor Targets / J. Garz=n ; I. de Antonio ; P. Sfnchez-Blfzquez
In vivo Modulation of G-proteins and Opioid Receptor Function by Antisense Oligodeoxynucleotides / J. Karle ; M. Nielsen
Targeting Brain GABA-A Receptors with Antisense Oligonucleotides: Implications for Epilepsy / D. Mohuezy ; X. Tang ; M.I. Phillips
Pathway of Allosteric Control as Revealed by Intermediate States of Hemoglobin / J.M. Holt ; G.K. Ackers
Probes of Energy Transduction in Enzyme Catalysis / Y. Huang ; D.W. Bolen
Macromolecules and Water: Probing with Osmotic Stress / V.A. Parsegian ; R.P. Rand ; D.C. Rau
Linkage of Protein Assembly to Protein-DNA Binding / I. Wong ; T.M. Lohman
Linkage at Steady State: Allosteric Transitions of Thrombin / E. Di Cera ; Q.D. Dang ; Y. Ayala ; A. Vindigni
Thermal Denaturation Methods in Study of Protein Folding / E. Freire
Kinetics of Lipid Membrane Phase Transitions: A Volume Perturbation Calorimeter Study / L. Chen ; R.L. Biltonen ; M.L. Johnson
Tight Binding Affinities Determined from the Thermodynamic Linkage to Protons by Titration Calorimetry / M.L. Doyle ; G. Louie ; P. Dal Monte ; T. Sokoloski
Calorimetric Methods for Interpreting Protein-Ligand Interactions / H.F. Fisher ; N. Singh
Extracting Thermodynamic Data from Equilibrium Melting Curves for Oligonucleotide Order-Disorder Transitions / K.J. Breslauer
Thermodynamics and Mutations in RNA-Protein Interactions / K.B. Hall ; J.K. Kranz
Melting Studies of RNA Unfolding and RNA-Ligand Interactions / D.E. Draper ; T.C. Gluick
Structural-Perturbation Approaches to Thermodynamics of Site-Specific Protein-DNA Interactions / L. Jen-Jacobson
Thermodynamic Parameters from Hydrogen Exchange Measurements / Y. Bai ; J.J. Englander ; L. Mayne ; J.S. Milne ; S.W. Englander
Application of Pressure to Biochemical Equilibria: The Other Thermodynamic Variable / C.A. Royer
Molecular Volume / L.M. Rellick ; W.J. Becktel
Hydrostatic and Osmotic Pressure as Tools to Study Macromolecular Recognition / C.R. Robinson ; S.G. Sligar
Sedimentation Equilibrium as Thermodynamic Tool / T.M. Laue
Footprint Phenotypes: Structural Models of DNA-Binding Proteins from Chemical Modification Analysis of DNA / J. Yang ; J. Carey
Low-Temperature Electrophoresis Methods / M. Perrella ; I. Denisov
Use of Multiple Spectroscopic Methods to Monitor Equilibrium Unfolding of Proteins / M.R. Eftink
Probing Structural and Physical Basis of Protein Energetics Linked to Protons and Salt / B. Garcia-Moreno
Evaluating Contribution of Hydrogen Bonding and Hydrophobic Bonding to Protein Folding / C.N. Pace
Analyzing Solvent Reorganization and Hydrophobicity / B. Lee
Simple Force Field for Study of Peptide and Protein Conformational Properties / T.P. Creamer ; G.D. Rose
Probes for Analysis of Stability of Different Variants of Aspartate Aminotransferase / M. Martinez-Carrion ; A. Artigues ; A. Berezov ; M.L. Bianconi ; A.M. Reyes ; A. Iriarte
Gene Fusions as Reporters of Gene Expression in Prokaryotic Cells / Section II.：
A Practical Guide to the Construction and Use of lac Fusions in Escherichia coli / Nicholas J. Hand ; Thomas J. Silhavy2.：
Tagging Exported Proteins Using Escherichia coli Alkaline Phosphatase Gene Fusions / Colin Manoil3.：
Applications of Gene Fusions to Green Fluorescent Protein and Flow Cytometry to the Study of Bacterial Gene Expression in Host Cells / Raphael H. Valdivia ; Lalita Ramakrishnan4.：
IVET and RIVET: Use of Gene Fusions to Identity Bacterial Virulence Factors Specifically Induced in Host Tissues / James M. Slauch ; Andrew Camilli5.：
Identification of Exported Bacterial Proteins via Gene Fusions to Yersinia pseudotuberculosis Invasin / Micah J. Worley ; Fred Heffron6.：
Gene Fusions as Reporters of Gene Expression in Eukaryotic Cells / Section III.：
Use of Imidazoleglycerolphosphate Dehydratase (His3) as a Biological Reporter in Yeast / Joe Horecka ; George F. Sprague, Jr.7.：
Use of Fusions to Human Thymidine Kinase as Reporters of Gene Expression and Protein Stability in Saccharomyces cerevisiae / Sofie Reda Salama8.：
Use of Fusions to Thymidine Kinase / Christiaan Karreman9.：
In Vivo Analysis of lacZ Fusion Genes in Transgenic Drosophila melanogaster / Stephen Small10.：
Utility of the Secreted Placental Alkaline Phosphatase Reporter Enzyme / Bryan R. Cullen11.：
Fusions to Imidazopyrazinone-Type Luciferases and Aequorin as Reporters / Satoshi Inouye12.：
Novel Methods for Chemiluminescent Detection of Reporter Enzymes / Corinne E. M. Olesen ; Yu-Xin Yan ; Betty Liu ; Dina Martin ; Brian D'Eon ; Ray Judware ; Chris Martin ; John C. Voyta ; Irena Bronstein13.：
Fusions to Chloramphenicol Acetyltransferase as a Reporter / Clayton Bullock ; Cornelia Gorman14.：
Fusions to [beta]-Lactamase as a Reporter for Gene Expression in Live Mammalian Cells / Gregor Zlokarnik15.：
Functional Tags for Biochemical Purification / Section IV.：
Purification of Proteins Using Polyhistidine Affinity Tags / Joshua A. Bornhorst ; Joseph J. Falke16.：
Generating Fusions to Glutathione S-Transferase for Protein Studies / Donald B. Smith17.：
Use of the Strep-Tag and Streptavidin for Detection and Purification of Recombinant Proteins / Arne Skerra ; Thomas G. M. Schmidt18.：
Streptavidin-Containing Chimeric Proteins: Design and Production / Takeshi Sano ; Charles R. Cantor19.：
Fusions to Maltose-Binding Protein: Control of Folding and Solubility in Protein Purification / Deepali Sachdev ; John M. Chirgwin20.：
Thioredoxin as a Fusion Partner for Production of Soluble Recombinant Proteins in Escherichia coli / Edward R. LaVallie ; Zhijian Lu ; Elizabeth A. Diblasio-Smith ; Lisa A. Collins-Racie ; John M. McCoy21.：
Affinity Purification of Recombinant Proteins Fused to Calmodulin or to Calmodulin-Binding Peptides / Peter Vaillancourt ; Chao-Feng Zheng ; Danny Q. Hoang ; Lisa Breister22.：
The S Tag Fusion System for Protein Purification / Ronald T. Raines ; Mark McCormick ; Thomas R. Van Oosbree ; Robert C. Mierendorf23.：
Fusions to Self-Splicing Inteins for Protein Purification / Ming-Qun Xu ; Henry Paulus ; Shaorong Chong24.：
Fusion Proteins Containing Cellulose-Binding Domains / Jae-Seon Park ; Hae-Sun Shin ; Roy H. Doi25.：
Biotinylation of Proteins in Vivo and in Vitro Using Small Peptide Tags / Millard G. Cull ; Peter J. Schatz26.：
Biotinylation of Proteins in Vivo: A Useful Post-translational Modification for Protein Analysis / John E. Cronan, Jr. ; Kelynne E. Reed27.：
Hybrid Proteins for Detection and Production of Antigens and Antibodies / Section V.：
Methods for Generating Multivalent and Bispecific Antibody Fragments / Ian Tomlinson ; Philipp Holliger28.：
Design and Use of Phage Display Libraries for the Selection of Antibodies and Enzymes / Francesca Viti ; Fredrik Nilsson ; Salvatore Demartis ; Adrian Huber ; Dario Neri29.：
Use of an Lpp-OmpA Fusion Vehicle for Bacterial Surface Display / Charles F. Earhart30.：
Identification of Bacterial Class I Accessible Proteins by Disseminated Insertion of Class I Epitopes / Dolph Ellefson ; Adrianus W. M. van der Velden ; David Parker31.：
Bordetella pertussis Adenylate Cyclase Toxin: A Vehicle to Deliver CD8-Positive T-Cell Epitopes into Antigen-Presenting Cells / Pierre Guermonprez ; Catherine Fayolle ; Gouzel Karimova ; Agnes Ullmann ; Claude Leclerc ; Daniel Ladant32.：
Use of Anthrax Toxin Fusions to Stimulate Immune Responses / Christopher C. Zarozinski ; R. John Collier ; Michael N. Starnbach33.：
Use of Fusions to Viral Coat Proteins as Antigenic Carriers for Vaccine Development / Peter G. Stockley ; Robert A. Mastico34.：
Epitope Tagging: General Method for Tracking Recombinant Proteins / Christian E. Fritze ; Thomas R. Anderson1.：
Markers for Cytology, Analysis of Protein Trafficking, and Lineage Tracing / Section II.：
Alkaline Phosphatase Fusions of Ligands or Receptors as in Situ Probes for Staining of Cells, Tissues, and Embryos / John G. Flanagan ; Hawai-Jong Cheng ; David A. Feldheim ; Mitsuharu Hattori ; Qiang Lu ; Pierre Vanderhaeghen2.：
Chimeric Molecules Employing Horseradish Peroxidase as Reporter Enzyme for Protein Localization in the Electron Microscope / Colin Hopkins ; Adele Gibson ; Jane Stinchcombe ; Clare Futter3.：
Biochemical Analyses of Trafficking with Horseradish Peroxidase-Tagged Chimeras / Anastasiya D. Blagoveshchenskaya ; Daniel F. Cutler4.：
Visualizing Protein Dynamics in Yeast with Green Fluorescent Protein / Christopher G. Burd5.：
Kinetic Analysis of Intracellular Trafficking in Single Living Cells with Vesicular Stomatitis Virus Protein G-Green Fluorescent Protein Hybrids / Koret Hirschberg ; Robert D. Phair ; Jennifer Lippincott-Schwartz6.：
Dual Color Detection of Cyan and Yellow Derivatives of Green Fluorescent Protein Using Conventional Fluorescence Microscopy and 35-mm Photography / Gisele Green ; Steven R. Kain ; Brigitte Angres7.：
Invertase Fusion Proteins for Analysis of Protein Trafficking in Yeast / Tamara Darsow ; Greg Odorizzi ; Scott D. Emr8.：
Introduction of Kex2 Cleavage Sites in Fusion Proteins for Monitoring Localization and Transport in Yeast Secretory Pathway / B. Diane Hopkins ; Ken Sato ; Akihiko Nakuno ; Todd R. Graham9.：
Lineage Analysis with Retroviral Vectors / Constance L. Cepko ; Elizabeth Ryder ; Christopher Austin ; Jeffrey Golden ; Shawn Fields-Berry ; John Lin10.：
Use of Pseudotyped Retroviruses in Zebrafish as Genetic Tags / Shawn Burgess ; Nancy Hopkins11.：
Tools for Analysis of Membrane Proteins / Section III.：
A Gene Fusion Method for Assaying Interactions of Protein Transmembrane Segments in Vivo / Jennifer A. Leeds ; Jon Beckwith12.：
Using SUC2-HIS4C Reporter Domain to Study Topology of Membrane Proteins in Saccharomyces cerevisiae / Christian Sengstag13.：
Detecting Interactions between Membrane Proteins in Vivo Using Chimeras / Igor Stagljar ; Stephan te Heesen14.：
Alkaline Phosphatase Fusion Proteins for Molecular Characterization and Cloning of Receptors and Their Ligands / Hwai-Jong Cheng15.：
Surface Chimeric Receptors as Tools in Study of Lymphocyte Activation / Bryan A. Irving ; Arthur Weiss16.：
Use of Chimeric Receptor Molecules to Dissect Signal Transduction Mechanisms / Warren J. Leonard17.：
Fusion Protein Toxins Based on Diphtheria Toxin: Selective Targeting of Growth Factor Receptors of Eukaryotic Cells / Johanna C. vanderSpek ; John R. Murphy18.：
Green Fluorescent Protein-Based Sensors for Detecting Signal Transduction and Monitoring Ion Channel Function / Micah S. Siegel ; Ehud Y. Isacoff19.：
Metabolic Labeling of Glycoproteins with Chemical Tags through Unnatural Sialic Acid Biosynthesis / Christina L. Jacobs ; Kevin J. Yarema ; Lara K. Mahal ; David A. Nauman ; Neil W. Charters ; Carolyn R. Bertozzi20.：
Signals for Addressing Proteins to Specific Subcellular Compartments / Section IV.：
Using Sorting Signals to Retain Proteins in Endoplasmic Reticulum / Hugh R. B. Pelham21.：
Directing Proteins to Nucleus by Fusion to Nuclear Localization Signal Tags / Heike Krebber ; Pamela A. Silver22.：
Identification, Analysis, and Use of Nuclear Export Signals in Saccharomyces cerevisiae / Karsten Weis23.：
Directing Proteins to Mitochondria by Fusion to Mitochondrial Targeting Signals / Kostas Tokatlidis24.：
Targeting Proteins to Plasma Membrane and Membrane Microdomains by N-Terminal Myristoylation and Palmitoylation / Wouter van't Hof ; Marilyn D. Resh25.：
Analysis of Function and Regulation of Proteins That Mediate Signal Transduction by Use of Lipid-Modified Plasma Membrane-Targeting Sequences / Gary W. Reuther ; Janice E. Buss ; Lawrence A. Quilliam ; Geoffrey J. Clark ; Channing J. Der26.：
Glycerolphosphoinositide Anchors for Membrane-Tethering Proteins / John D. Fayen ; Mark L. Tykocinski ; M. Edward Medof27.：
Fusions to Members of Fibroblast Growth Factor Gene Family to Study Nuclear Translocation and Nonclassic Exocytosis / I. Prudovsky ; M. Landriscina ; R. Soldi ; S. Bellum ; D. Small ; V. Andreeva ; Thomas Maciag28.：
Application of Chimeras in Monitoring and Manipulating Cell Physiology / Section V.：
Posttranslational Regulation of Proteins by Fusions to Steroid-Binding Domains / Didier Picard29.：
Tet Repressor-Based System for Regulated Gene Expression in Eukaryotic Cells: Principles and Advances / Udo Baron ; Hermann Bujard30.：
Coumermycin-Induced Dimerization of GyrB-Containing Fusion Proteins / Michael A. Farrar ; Steven H. Olson ; Roger M. Perlmutter31.：
Use of Glutathione S-Transferase and Break Point Cluster Region Protein as Artificia Dimerization Domains to Activate Tyrosine Kinases / Yoshiro Maru32.：
Recombinant Aequorin as Tool for Monitoring Calcium Concentration in Subcellular Compartments / Valerie Robert ; Paolo Pinton ; Valeria Tosello ; Rosario Rizzuto ; Tullio Pozzan33.：
Recombinant Aequorin as Reporter of Changes in Intracellular Calcium in Mammalian Cells / Jenny Stables ; Larry C. Mattheakis ; Ray Chang ; Stephen Rees34.：
Monitoring Protein Conformations and Interactions by Fluorescence Resonance Energy Transfer between Mutants of Green Fluorescent Protein / Atsushi Miyawaki ; Roger Y. Tsien35.：
Studies of Signal Transduction Events Using Chimeras to Green Fluorescent Protein / Tobias Meyer ; Elena Oancea36.：
Use of Fusions to Green Fluorescent Protein in the Detection of Apoptosis / Eve Shinbrot ; Collin Spencer ; Valerie Natale37.：
Synapto-pHluorins: Chimeras between pH-Sensitive Mutants of Green Fluorescent Protein and Synaptic Vesicle Membrane Proteins as Reporters of Neurotransmitter Release / Rafael Yuste ; Rebecca B. Miller ; Knut Holthoff ; Shifang Zhang ; Gero Miesenbock38.：
Studying Organelle Physiology with Fusion Protein-Targeted Avidin and Fluorescent Biotin Conjugates / Minnie M. Wu ; Juan Llopis ; Stephen R. Adams ; J. Michael McCaffery ; Ken Teter ; Markku S. Kulomaa ; Terry E. Machen ; Hsiao-Ping H. Moore39.：
Fluorescent Labeling of Recombinant Proteins in Living Cells with FlAsH / B. Albert Griffin ; Jay Jones40.：
Ubiquitin Fusion Technique and Its Descendants / Alexander Varshavsky41.：
Use of Phosphorylation Site Tags in Proteins / Sidney Pestka ; Lei Lin ; Wei Wu ; Lara Izotova42.：
Computational Analyses of Sequences and Sequence Alignments / Section I：
Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis / Chapter 1：
Two component systems in microbial communities: Approaches and resources for analyzing metagenomic data sets / Chapter 2：
Identification of sensory and signal-transducing domains in two-component signaling systems / Chapter 3：
Features of protein-protein interactions in two-component signaling deduced from genomic libraries / Chapter 4：
Sporulation phosphorelay proteins and their complexes: Crystallographic characterization / Chapter 5：
Control Analysis of Bacterial Chemotaxis Signaling / Chapter 6：
Classification of Response Regulators based on their Surface Properties / Chapter 7：
Biochemical and Genetic Assays of Individual Components of the Signaling Systems / Section II：
Purification and Assays of Rhodobacter capsulatus RegB-RegA Two Component Signal Transduction System / Chapter 8：
Purification and reconstitution of PYP-phytochrome (Ppr) with biliverdin and 4-hydroxycinnamic acid / Chapter 9：
Oxygen and Redox-Sensing by Two-Component Systems That Regulate Behavioral Responses. Behavioral Assays and Structural Studies of Aer using in vivo Disulfide Crosslinking / Chapter 10：
Two-Component Signaling in the Virulence of S. aureus: A Silkworm Larvae-Pathogenic Agent Infection Model of Virulence / Chapter 11：
TonB System, In vivo Assays and Characterization / Chapter 12：
Biochemical Characterization of Plant Ethylene Receptors following Transgenic Expression in Yeast / Chapter 13：
Structure of SixA, a histidine protein phosphatase of the ArcB HPt domain in E. coli / Chapter 14：
Triggering and monitoring light-sensing reactions in protein crystals / Chapter 15：
Application of Fluorescence Resonance Energy Transfer to Examine EnvZ/OmpR Interactions / Chapter 16：
Gene promoter scan (GPS) methodology for identifying and classifying co-regulated promoters / Chapter 18：
Targeting two-component signal transduction: A novel drug discovery system / Chapter 19：
The essential YycFG twocomponent system of Bacillus subtilis / Chapter 20：
Physiological Assays and Readouts / Section III：
Isolation and Characterization of Chemotaxis Mutants of the Lyme Disease Spirochete Borrelia burgdorferi Using Allelic Exchange Mutagenesis, Flow Cytometry and Cell Tracking / Chapter 21：
Phosphorylation assays of chemotaxis two-component system proteins in Borrelia burgdorferi / Chapter 22：
Regulation of Respiratory Genes by ResD-ResE Signal Transduction System in Bacillus subtilis / Chapter 23：
Physiological and genetic characterization of two-component systems in Myxococcus / Chapter 24：
Detection and measurement of two-component systems that control dimorphism and virulence in fungi / Chapter 25：
Using Two-Component Systems and other Bacterial Regulatory Factors for the Fabrication of Synthetic Genetic Devices / Chapter 26：
Computational Analyses of Sequences and Sequence Alignments / Section I：
Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis / Chapter 1：
Two component systems in microbial communities: Approaches and resources for analyzing metagenomic data sets / Chapter 2：
The PICM Chemical Scanning Method for Identifying Domain-Domain and Protein-Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis / Randal B. Bass ; Aaron S. Miller ; Susan L. Gloor ; Joseph J. Falke1：
Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics: Applications to Soluble and Transmembrane Receptors of Bacterial Chemotaxis / Scott L. Butler ; Stephen A. Chervitz2：
Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine Kinases / Peter P. Borbat ; Jack H. Freed3：
Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR / Jaya Bhatnagar ; Brian R. Crane4：
TonB/TolA Amino-Terminal Domain Modeling / Kimberly L. Keller ; Kerry K. Brinkman ; Ray A. Larsen5：
Functional Dynamics of Response Regulators Using NMR Relaxation Techniques / Alexandra K. Gardino ; Dorothee Kern6：
The Design and Development of Tar-EnvZ Chimeric Receptors / Takeshi Yoshida ; Sangita Phadtare ; Masayori Inouye7：
Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli / 8：
Light Modulation of Histidine-Kinase Activity in Bacterial Phytochromes Monitored by Size Exclusion Chromatography, Crosslinking, and Limited Proteolysis / Steffi Noack ; Tilman Lamparter9：
A Temperature-Sensing Histidine Kinase-Function, Genetics, and Membrane Topology / Yvonne Braun ; Angela V. Smirnova ; Helge Weingart ; Alexander Schenk ; Matthias S. Ullrich10：
The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal Molecules / Matthew B. Neiditch ; Frederick M. Hughson11：
Reconstitution of Heterogeneous Systems / Section II：
Liposome-Mediated Assembly of Receptor Signaling Complexes / David J. Montefusco ; Abdalin E. Asinas ; Robert M. Weis12：
Analyzing Transmembrane Chemoreceptors Using In Vivo Disulfide Formation Between Introduced Cysteines / Wing-Cheung Lai ; Gerald L. Hazelbauer13：
Using Nanodiscs to Create Water-Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers / Thomas Boldog ; Mingshan Li14：
Assays for CheC, FliY, and CheX as Representatives of Response Regulator Phosphatases / Travis J. Muff ; George W. Ordal15：
Genetic Dissection of Signaling Through the Rcs Phosphorelay / Nadim Majdalani ; Susan Gottesman16：
Intracellular Methods and Assays / Section III：
In Vivo Measurement by FRET of Pathway Activity in Bacterial Chemotaxis / Victor Sourjik ; I'd Vaknin ; Thomas S. Shimizu ; Howard C. Berg17：
In Vivo and In Vitro Analysis of the Rhodobacter sphaeroides Chemotaxis Signaling Complexes / Steven L. Porter ; George H. Wadhams ; Judith P. Armitage18：
In Vivo Crosslinking Methods for Analyzing the Assembly and Architecture of Chemoreceptor Arrays / Claudia A. Studdert ; John S. Parkinson19：
A "Bucket of Light" for Viewing Bacterial Colonies in Soft Agar / 20：
Phenotypic Suppression Methods for Analyzing Intra- and Inter-Molecular Signaling Interactions of Chemoreceptors / Peter Ames21：
Single-Cell Analysis of Gene Expression by Fluorescence Microscopy / Tim Miyashiro ; Mark Goulian22：
Genome-Wide Analyses of Two-Component Systems / Section IV：
Two-Component Systems of Mycobacterium tuberculosis-Structure-Based Approaches / Paul A. Tucker ; Elzbieta Nowak ; Jens Preben Morth23：
Transcriptomic Analysis of ArlRS Two-Component Signaling Regulon, a Global Regulator, in Staphylococcus aureus / Yinduo Ji ; Chuanxin Yu ; Xudong Liang24：
Global Analysis of Two-Component Gene Regulation in H. pylori by Mutation Analysis and Transcriptional Profiling / Biju Joseph ; Dagmar Beier25：
Phosphotransfer Profiling: Systematic Mapping of Two-Component Signal Transduction Pathways and Phosphorelays / Michael T. Laub ; Emanuele G. Biondi ; Jeffrey M. Skerker26：
Identification of Histidine Phosphorylations in Proteins Using Mass Spectrometry and Affinity-Based Techniques / Andrew R. S. Ross27：
Analysis of Gene Function using DNA Microarrays / Andrew P. Capaldi1：
An introduction to microarray data analysis and visualization / Gregg B. Whitworth2：
Genome-wide approaches to monitor pre-mRNA splicing / Maki Inada ; Jeff Pleiss3：
ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites / Philippe Lefrançois ; Wei Zheng ; Michael Snyder4：
Genome-wide mapping of nucleosomes in yeast / Oliver J. Rando5：
Genome-wide translational profiling by ribosome footprinting / Nicholas T. Ingolia6：
Systematic Genetic Analysis / Part II：
Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe / Anastasia Baryshnikova ; Michael Costanzo ; Scott Dixon ; Franco J. Vizeacoumar ; Chad L. Myers ; Brenda Andrews ; Charles Boone7：
Making temperature-sensitive mutants / Shay Ben-Aroya ; Xuewen Pan ; Jef D. Boeke ; Philip Hieter8：
Quantitative Genetic Interaction Mapping using the E-MAP Approach / Sean R. Collins ; Assen Roguev ; Nevan J. Krogan9：
Exploring gene function and drug action using chemogenomic dosage assays / Elke Ericson ; Shawn Hoon ; Robert P. St. Onge ; Guri Giaever ; Corey Nislow10：
Proteomics / Part III：
Yeast Expression Proteomics by High-Resolution Mass Spectrometry / Tobias C. Walther ; Jesper V. Olsen ; Matthias Mann11：
High-quality binary interactome mapping / Matija Dreze ; Dario Monachello ; Claire Lurin ; Michael E. Cusick ; David E. Hill ; March Vidal ; Pascal Braun12：
Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics / Bernd Bodenmiller ; Rudi Aebersold13：
A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells / Stephen W. Michnick ; Po-Hien Ear ; Christian Landry ; Mohan K. Malleshaiah ; Vincent Messier14：
Yeast Lipid Analysis and Quantification by Mass Spectrometry / Xue Li Guan ; Isabelle Riezman ; Markus R. Wenk ; Howard Riezman15：
Mass Spectrometry-Based Metabolomics of Yeast / Christopher A. Crutchfield ; Wenyun Lu ; Eugene Melamud ; Joshua D. Rabinowitz16：
Systems Analysis / Part IV：
Imaging single mRNA molecules in yeast / Hyun Youk ; Arjun Raj ; Alexander van Oudenaarden17：
Experimental evolution in yeast: a practical guide / Maitreya J. Dunham19：
Synthetic Biology / Part V：
Enhancing stress resistance and production phenotypes through transcriptome engineering / Felix H. Lam ; Franz S. Hartner ; Gerald R. Fink ; Gregory Stephanopoulos20：
Advances in Cytology/Biochemistry / Part VI：
Visualizing yeast chromosomes and nuclear architecture / Peter Meister ; Lutz R. Gehlen ; Elisa Varela ; Veronique Kalck ; Susan M. Gasser21：
Quantitative localization of chromosomal loci by immunofluorescence / Donna Garvey Brickner ; William Light ; Jason H. Brickner22：
Spinning Disk Confocal Microscopy of Yeast / Kurt Thorn23：
Correlative GFP-immunoelectron microscopy in yeast / Christopher Buser ; Kent McDonald24：
Analyzing P-bodies and stress granules in Saccharomyces cerevisiae / J Ross Buchan ; Tracy Nissan ; Roy Parker25：
Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization / Daniel Zenklusen ; Robert H Singer26：
The Use of In Vitro Assays to Measure Endoplasmic Reticulum Associated Degradation (ERAD) / Jeffrey L. Brodsky27：
A protein transformation protocol for introducing yeast prion Particles into yeast / Motomasa Tanaka28：
Overexpression and Purification of Integral Membrane Proteins in Yeast / Franklin A. Hays ; Zygy Roe-Zurz ; Robert M. Stroud29：
Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeast / Simon Alberti ; Randal Halfmann ; Susan Lindquist30：
Other Fungi / Part VII：
Genetics and Molecular Biology in Candida albicans / Hernday, A.D. ; Noble, S. M. ; Mitrovich, Q.M. ; Johnson, A.D31：
Molecular Genetics of Schizosaccharomyces pombe / Sarah A. Sabatinos ; Susan L. Forsburg32：
Applying Genetics and Molecular Biology to the Study of the Human Pathogen Cryptococcus neoformans / Cheryl D. Chun ; Hiten D. Madhani33：
The Fungal Genome Initiative and lessons learned from genome sequencing / Christina A. Cuomo ; Bruce W. Birren34：
Ultradian metabolic cycles in yeast / Benjamin Tu35：
Functional Genomics / Part I：
Analysis of Gene Function using DNA Microarrays / Andrew P. Capaldi1：
An introduction to microarray data analysis and visualization / Gregg B. Whitworth2：
Measurement and Analysis of Equilibrium Binding Titrations: A beginner's guide / Dorothy Beckett
Macromolecular Competition Titration Method: Accessing Thermodynamics of the Unmodified Macromolecule - Ligand Interactions Through Spectroscopic Titrations of Fluorescent Analogs / Wlodzimierz Bujalowski ; Maria J. Jezewska
Analysis of PKR-RNA interactions by sedimentation velocity / C. Jason Wong ; Katherine Launer-Felty ; James L. Cole
Structural and Thermodynamic Analysis of PDZ/Ligand Interactions / Tyson R. Shepherd ; Ernesto J. Fuentes
Thermodynamic analysis of metal ion-induced protein assembly / Andrew B. Herr ; Deborah G. Conrady
Thermodynamic Dissection of Colicin Interactions / Nicholas G Housden ; Colin Kleanthous
Energetics of Src homology domain interactions in receptor tyrosine kinase-mediated signaling / John E. Ladbury ; Stefan T. Arold
Structural and Functional Energetic Linkages in Allosteric Regulation of Muscle Pyruvate Kinase / J. Ching Lee ; Petr Herman
Analysis of Free Energy versus Temperature Curves in Protein Folding and Macromolecular Interactions / Vince J. LiCata ; Chin-Chi Liu
Application of the Sequential n-Step Kinetic Mechanism to Polypeptide Translocases / Aaron L. Lucius ; Justin M. Miller ; Burki Rajendar
A Coupled Equilibrium Approach to Study Nucleosome Thermodynamics / Andrew J. Andrews ; Karolin Luger
Quantitative methods for measuring DNA flexibility in vitro and in vivo / Justin P. Peters ; Nicole Becker ; Emily M. Rueter ; Zeljko Bajzer ; Jason D. Kahna ; L. James Maher
A Thermodynamic Approach for the Targeting of Nucleic Acid Structures Using Their Complementary Single Strands / Hui-Ting Lee ; Caroline Carr ; Hollie Siebler ; Lela Waters ; Irine Khutsishvili ; Fany Iseka ; Brian Domack ; Chris M. Olsen ; Luis Marky
Thermodynamics of Biological Processes / Hernan G. Garcia ; Jane Kondev ; Nigel Orme ; Julie Theriot ; Rob Phillips
Protein stability in the presence of co-solutes / Luis Marcelo F. Holthauzen ; Matthew Auton ; Mikhail Sineva ; Jorg Rosgen
Small Angle X-ray Scattering Studies of Peptide-Lipid Interactions using the Mouse Paneth Cell a-Defensin Cryptdin-4 / Abhijit Mishra ; Kenneth P. Tai ; Nathan W. Schmidt ; André J. Ouellette ; Gerard C. L. Wong
Synergy of molecular dynamics and isothermal titration calorimetry in studies of allostery / Rebecca Strawn ; Thomas Stockner ; Milan Melichercik ; Lihua Jin ; Wei-Feng Xue ; Jannette Carey ; Rüdiger Ettrich
Using tryptophan fluorescence to measure the stability of membrane proteins folded in liposomes / C. Preston Moon1 ; Karen G. Fleming
Non-B Conformations of CAG Repeats Using 2-aminopurine / Natalya N. Degtyareva ; Jeffrey T. Petty
Disulfide bond-mediated passenger domain stalling as a structural probe of autotransporter outer membrane secretion in vivo / Jonathan P. Renn ; Patricia L. Clark
Strategies for the Thermodynamic Characterization of Linked Binding/Local Folding Reactions Within the Native State: Application to the LID Domain of Adenylate Kinase from Escherichia coli / Travis P. Schrank ; W. Austin Elam ; Jing Li ; Vincent J. Hilser
Measurement and Analysis of Equilibrium Binding Titrations: A beginner's guide / Dorothy Beckett
Macromolecular Competition Titration Method: Accessing Thermodynamics of the Unmodified Macromolecule - Ligand Interactions Through Spectroscopic Titrations of Fluorescent Analogs / Wlodzimierz Bujalowski ; Maria J. Jezewska
Analysis of PKR-RNA interactions by sedimentation velocity / C. Jason Wong ; Katherine Launer-Felty ; James L. Cole
Predicting Fluorescence Lifetimes and Spectra of Biopolymers / Patrik R. Callis
Modeling of Regulatory Networks: Theory and Applications in the study of the Drosophila Circadian Clock / Elizabeth Y. Scribner ; Hassan M. Fathallah-Shaykh
Strategies for articulated multibody-based adaptive coarse grain simulation of RNA / Mohammad Poursina ; Kishor D. Bhalerao ; Samuel C. Flores ; Kurt S. Anderson ; Alain Laederach
Modeling Loop Entropy / Gregory S. Chirikjian
Inferring Functional Relationships and Causal Network Structure from Gene Expression Profiles / Radhakrishnan Nagarajan
Numerical solution of the chemical master equation: uniqueness and stability of the stationary distribution for chemical networks, and mRNA bursting in a gene network with negative feedback regulation / E. S. Zeron ; M. Santill_an
How molecular should your molecular model be? On the level of molecular detail required to simulate biological networks in systems and synthetic biology / Didier Gonze ; Wassim Abou-Jaoud´e ; Adama Ouattara ; Jos´e Halloy
Computational modelling of biological pathways by executable biology / Maria Luisa Guerriero John K. Heath
Computing Molecular Fluctuations in Biochemical Reaction Systems Based on A Mechanistic, Statisitical Theory of Irreversible Processes / Don Kulasiri
Probing the input-output behaviour of biochemical and genetic systems: system identification methods from control theory / David McMillen ; Brian Ingalls ; Jordan Ang
Biochemical pathway modelling tools for drug target detection in cancer and other complex diseases / Alberto Marin-Sanguino ; Shailendra K. Gupta ; Eberhard O. Voit ; Julio Vera
Deterministic and Stochastic Simulation and Analysis of Biochemical Reaction Networks: The Lactose Operon Example / Necmettin Yildirim ; Caner Kazanci F.
Multivariate Neighborhood Sample Entropy: A method for data reduction and prediction of complex data / Joshua S. Richman MD, PhD
Scaling differences of heartbeat excursions between wake and sleep periods / L. Guzm´an-Vargas ; I. Reyes-Ram´irez ; R. Hern´andez-P´erez ; F. Angulo-Brown
Changepoint analysis for single-molecule polarized totalinternal reflection fluorescence microscopy experiments / John F. Beausang ; Yale E. Goldman ; Philip C. Nelson
Inferring mechanisms from dose-response curves / Carson C. Chow ; Karen M. Ong ; Edward J. Dougherty ; S. Stoney Simons, Jr
Spatial Aspects in Biological System Simulations / Haluk Resat ; Michelle N. Costa ; Harish Shankaran
Computational Approaches to Modeling Viral Structure and Assembly / Stephen C. Harvey ; Anton S. Petrov ; Batsal Devkota ; Mustafa Burak Boz
An Object-Oriented Software Suite for the Simulation and Design of Macromolecules / Andrew Leaver-Fay ; Michael Tyka ; Steven M. Lewis ; Oliver F. Lange ; James Thompson ; Ron Jacak ; Kristian Kaufman ; P. Douglas Renfrew ; Colin A. Smith ; Will Sheffler ; Ian W. Davis ; Seth Cooper ; Adrien Treuille ; Daniel J. Mandell ; Florian Richter ; Yih-En Andrew Ban ; Sarel J. Fleishman ; Jacob E. Corn ; David E. Kim ; Sergey Lyskov ; Monica Berrondo ; Stuart Mentzer ; Zoran Popovic ; James J. Havranek ; John Karanicolas ; Rhiju Das ; Jens Meiler ; Tanja Kortemme ; Jeffrey J. Gray ; Brian Kuhlman1 ; David Baker ; Philip Bradley
Computational Design of Intermolecular Stability and Specificity in Protein Self-Assembly / Vikas Nanda ; Sohail Zahid ; Fei Xu ; Daniel Levine
Predicting Fluorescence Lifetimes and Spectra of Biopolymers / Patrik R. Callis
Modeling of Regulatory Networks: Theory and Applications in the study of the Drosophila Circadian Clock / Elizabeth Y. Scribner ; Hassan M. Fathallah-Shaykh
Strategies for articulated multibody-based adaptive coarse grain simulation of RNA / Mohammad Poursina ; Kishor D. Bhalerao ; Samuel C. Flores ; Kurt S. Anderson ; Alain Laederach
Identification and characterization of Steroidogenic Factor-1 inverse agonists / Mabrouka Doghman ; Franck Madoux ; Peter Hodder ; Enzo Lalli
Assessment of inverse agonism for the angiotensin II type 1 receptor / Hiroshi Akazawa ; Noritaka Yasuda ; Shin-ichiro Miura ; Issei Komuro
Measurement of inverse agonism in BARs / Carlos Alberto Taira
Inverse agonism of antidepressants: in vitro and in vivo studies / Joel Bockaert
Differential inverse agonism at the human muscarinic M3 receptor / Paola Casarosa
Ghrelin Receptor: High Constitutive Activity and Methods for Developing Inverse Agonists / Constance Chollet
Constitutive activity and inverse agonism at the a1a and a1b adrenergic receptor subtypes / Susanna. Cotecchia
Measurement of inverse agonism of the cannabinoid receptor / Tung Fong
Constitutively Active Thyrotropin (TSH) and Thyrotropin-Releasing Hormone (TRH) Receptors and Their Inverse Agonists / Marvin C. Gershengorn
Inverse retinoid agonists and neutral antagonists / Hinrich Gronemeyer
y-Aminobutyric Acid Type A (GABAA) Receptor Subtype Inverse Agonists as Therapeutic Agents in Cognition / Gabriella Guerrini ; Ciciani Giovanna
Assays for inverse agonists in the visual system / Masahiro Kono
Receptor-driven identification of novel human A3 adenosine receptor antagonists as potential therapeutic agents / Silvia Paoletta ; Stephanie Federico ; Giampiero Spalluto ; Stefano Moro
Inverse agonists of steroidogenic factor SF-1 / Fabrice Piu ; Andria L. Del Tredici
Methods to Measure G Protein Coupled Receptor Activity for the Identification of Inverse Agonists / Gabriel Barreda-Gómez ; M.Teresa Giralt ; Rafael Rodríguez-Puertas
Novel Strategies and Techniques for Constitutive Activity and Inverse Agonism / Section II：
Use of Pharmacoperones to Reveal GPCR Structural Components Associated with Receptor Activation and Trafficking / Jo Ann Janovick ; P. Michael Conn
Application of large-scale transiently transfected cells to functional assays of ion channels and other proteins / Jun Chen ; Sujatha Gopalakrishnan ; Marc R Lake ; Bruce R Bianchi
Quantification of RNA Editing of the Serotonin 2C Receptor (5-HT2CR) Ex Vivo / Maria Fe Lanfranco ; Noelle C. Anastasio ; Patricia K. Seitz ; Kathryn A. Cunningham
Strategies for isolating constitutively-active and dominant-negative pheromone receptor mutants in yeast / Mercedes Dosil ; James B. Konopka
Development of a GPR23 Cell-based ß-Lactamase Reporter Assay / Paul H. Lee ; Bonnie J. Hanson
Computational Modeling of Constitutively Active Mutants of GPCRs: C5a Receptor / Gregory V. Nikiforovich ; Thomas J. Baranski
TSH receptor monoclonal antibodies with agonist, antagonist and inverse agonist activities / Jane Sanders ; Ricardo Núñez Miguel ; Jadwiga Furmaniak ; Bernard Rees Smith
Current standards, variations and pitfalls for the determination of constitutive TSHR activity in vitro / Sandra Mueller ; Holger Jaeschke ; Ralf Paschke
Towards the Rational Design of Constitutively Active KCa3.1 Mutant Channels / Line Garneau ; Hélène Klein ; Lucie Parent ; Rémy Sauvé
Fusion proteins as model systems for the analysis of constitutive GPCR activity / Erich H. Schneider ; Roland Seifert
Screening for Novel Constitutively Active CXCR2 Mutants and Their Cellular Effects / Giljun Park ; Tom Masi ; Chang K. Choi ; Heejung Kim ; Jeffrey M. Becker ; Tim E. Sparer
A method for parallel solid-phase synthesis of iodinated analogues of the cannabinoid receptor type I (CB1) inverse agonist rimonabant / Alan C. Spivey ; Chih-Chung Tseng
Coexpression systems as models for the analysis of constitutive GPCR activity
Modelling and simulation of inverse agonism dynamics / Lloyd J. Bridge
Design and Use of Constitutively Active STAT5 Constructs / Michael A. Farrar
In vitro and in vivo assays of protein kinase CK2 activity / Renaud Prudent ; Céline F. Sautel ; Virginie Moucadel ; Béatrice Laudet ; Odilhe Filhol ; Claude Cochet
Identification and characterization of Steroidogenic Factor-1 inverse agonists / Mabrouka Doghman ; Franck Madoux ; Peter Hodder ; Enzo Lalli
Assessment of inverse agonism for the angiotensin II type 1 receptor / Hiroshi Akazawa ; Noritaka Yasuda ; Shin-ichiro Miura ; Issei Komuro
Measurement of inverse agonism in BARs / Carlos Alberto Taira
Identification and Measurement of Constitutive Activity / Section I：
Constitutive activity at the cannabinoid CB1 receptor and behavioral responses / Katherine E. Hanlon ; Todd W. Vanderah
Detecting constitutive activity and protean agonism at the cannabinoid-2 receptor / Massimiliano Beltramo ; Rossella Brusa ; Isabella Mancini ; Paola Scandroglio ; Massimiliano. Beltramo
Modulation of constitutive activity of the ghrelin receptor by use of pharmacological tools as well as mutagenesis / Birgitte Holst ; Jacek Mokrosinski
Assessment of constitutive activity and internalization of GPR54 (Kiss1-r) / Macarena Pampillo ; Andy Videsh Babwah
Assessment of Constitutive Activity in EP Prostanoid Receptors / Hiromichi Fujino ; Toshihiko Murayama ; John W. Regan
a1D-Adrenergic receptors: constitutive activity and reduced expression at the plasma membrane / J Adolfo Garcia-Sáinz ; M. Teresa Romero-Ávila ; Luz del Carmen Medina
Constitutive activity of the histamine H1 receptor / Saskia Nijmeijer ; Rob Leurs ; Henry F Vischer
Assessment of homologous internalization of constitutively active N111G mutant of AT1 receptor / Mohiuddin Ahmed Bhuiyan ; Takafumi Nagatomo
Methods to detect cell surface expression and constitutive activity of GPR6 / Balakrishna M. Prasad ; Bettye Hollins ; Nevin A. Lambert
ß3-adrenoceptor agonists and (antagonists as) inverse agonists: history, perspective, constitutive activity and stereospecific binding / Maria Grazia Perrone ; Antonio Scilimati
Constitutive Activity of the Lutropin Receptor and its Allosteric Modulation by Receptor Heterodimerization / Deborah L. Segaloff
Assessing Constitutive Activity of Extracellular Calcium-Sensing Receptors in Vitro and in Bone / Wenhan Chang ; Melita Dvorak ; Dolores Shoback
Constitutive Activity of Neural Melanocortin Receptors / Ya-Xiong Tao ; Hui Huang ; Zhi-Qiang Wang ; Fan Yang ; Jessica N. Williams ; Gregory V. Nikiforovich
Measurement of Constitutive Activity of BMP Type I receptors / Peter ten Dijke
Probing the CA among dopamine D1 and D5 receptors and their mutants / Bianca Plouffe ; Jean-Philippe D'Aoust ; Vincent Laquerre ; Binhui Liang ; Mario Tiberi
Identification of Gain-of-Function Variants of the Human Prolactin Receptor / Vincent Goffin ; Roman L. Bogorad ; Philippe Touraine
Investigations of Activated ACVR1/ALK2, a Bone Morphogenetic Protein (BMP) Type I Receptor that Causes Fibrodysplasia Ossificans Progressiva / Frederick. Kaplan ; Petra Seemann ; Julia Haupt ; Meiqi Xu ; Vitali Lounev ; Mary Mullins ; Eileen M. Shore
Identification and evaluation of constitutively active thyroid stimulating hormone receptor mutations / Joaquin Lado-Abeal ; Leah R. Quisenberry ; Isabel Castro-Piedras
Assessment of constitutive activity of a G protein-coupled receptor, Cpr2, in Cryptococcus neoformans by heterologous and homologous methods / Chaoyang Xue ; Yina Wang ; Yen-Ping Hsueh
In vitro and in vivo assessment of mu opioid receptor constitutive activity / Edward J. Bilsky ; Denise Giuvelis ; Melissa D. Osborn ; Christina M. Dersch ; Heng Xu ; Richard B. Rothman
Constitutively active µ-opioid methods / Mark. Connor ; John Traynor
Protein Kinase Ck2 is a Constitutively-Active Enzyme That Promotes Cell Survival: Strategies To Identify Ck2 Substrates and Manipulate Its Activity In Mammalian Cells / Jacob P. Turowec ; James S. Duncan ; Ashley C. French ; Laszlo Gyenis ; Nicole A. St. Denis ; Greg J. Vilk ; David W. Litchfield
Assessment of Ck2 Constitutive Activity In Cancer Cells / Maria Ruzzene ; Giovanni Di Maira ; Kendra Tosoni ; Lorenzo A. Pinna
Structural basis of the constitutive activity of protein kinase CK2 / Birgitte B. Olsen ; Barbara Guerra ; Karsten Niefind ; Olar-Georg Issinger font error
Measuring the Constitutive Activation of c-Jun N-terminal Kinase Isoforms / Ryan T. Nitta ; Shawn S. Badal ; Albert J. Wong
Measurement of constitutive MAPK and PI3K/AKT signaling activity in human cancer cell lines / Keiran S.M. Smalley
Constitutive activity of GPR40/FFA1: Intrinsic or assay dependent? / Leigh A Stoddart ; Graeme Milligan
Constitutive activity of TRP channels: methods for measuring the activity and its outcome / Baruch Minke
Measurement of Orexin (Hypocretin) and Substance P Effects On Constitutively Active Inward Rectifier K+ Channels in Brain Neurons / Yasuko Nakajima ; Shigehiro Nakajima
Characterization of G protein receptor kinase 4 and measuring its constitutive activity in vivo / Bradley T. Andresen
Voltage-clamp based methods for the detection of constitutively active acetylcholine-gated IK,ACh channels in the diseased heart / Niels Voigt ; Samy Makary ; Stanley Nattel ; Dobromir Dobrev
Assaying Wave and WASH complex constitutive activities towards the Arp2/3 complex / Emmanuel Derivery ; Alexis Gautreau
Identification and Measurement of Constitutive Activity / Section I：
Constitutive activity at the cannabinoid CB1 receptor and behavioral responses / Katherine E. Hanlon ; Todd W. Vanderah
Detecting constitutive activity and protean agonism at the cannabinoid-2 receptor / Massimiliano Beltramo ; Rossella Brusa ; Isabella Mancini ; Paola Scandroglio ; Massimiliano. Beltramo
Alexandrine; Simon, Melvin I.; Crane, Brian; Crane, Alexandrine Crane, Alexandrine Crane, Brian R. Crane, Melvin I. Simon, Brian Crane, Michael I. Johnson
Computational Analyses of Sequences and Sequence Alignments / Section I：
Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis / Chapter 1：
Two component systems in microbial communities: Approaches and resources for analyzing metagenomic data sets / Chapter 2：
Identification of sensory and signal-transducing domains in two-component signaling systems / Chapter 3：
Features of protein-protein interactions in two-component signaling deduced from genomic libraries / Chapter 4：
Sporulation phosphorelay proteins and their complexes: Crystallographic characterization / Chapter 5：
Control Analysis of Bacterial Chemotaxis Signaling / Chapter 6：
Classification of Response Regulators based on their Surface Properties / Chapter 7：
Biochemical and Genetic Assays of Individual Components of the Signaling Systems / Section II：
Purification and Assays of Rhodobacter capsulatus RegB-RegA Two Component Signal Transduction System / Chapter 8：
Purification and reconstitution of PYP-phytochrome (Ppr) with biliverdin and 4-hydroxycinnamic acid / Chapter 9：
Oxygen and Redox-Sensing by Two-Component Systems That Regulate Behavioral Responses. Behavioral Assays and Structural Studies of Aer using in vivo Disulfide Crosslinking / Chapter 10：
Two-Component Signaling in the Virulence of S. aureus: A Silkworm Larvae-Pathogenic Agent Infection Model of Virulence / Chapter 11：
TonB System, In vivo Assays and Characterization / Chapter 12：
Biochemical Characterization of Plant Ethylene Receptors following Transgenic Expression in Yeast / Chapter 13：
Structure of SixA, a histidine protein phosphatase of the ArcB HPt domain in E. coli / Chapter 14：
Triggering and monitoring light-sensing reactions in protein crystals / Chapter 15：
Application of Fluorescence Resonance Energy Transfer to Examine EnvZ/OmpR Interactions / Chapter 16：
Gene promoter scan (GPS) methodology for identifying and classifying co-regulated promoters / Chapter 18：
Targeting two-component signal transduction: A novel drug discovery system / Chapter 19：
The essential YycFG twocomponent system of Bacillus subtilis / Chapter 20：
Physiological Assays and Readouts / Section III：
Isolation and Characterization of Chemotaxis Mutants of the Lyme Disease Spirochete Borrelia burgdorferi Using Allelic Exchange Mutagenesis, Flow Cytometry and Cell Tracking / Chapter 21：
Phosphorylation assays of chemotaxis two-component system proteins in Borrelia burgdorferi / Chapter 22：
Regulation of Respiratory Genes by ResD-ResE Signal Transduction System in Bacillus subtilis / Chapter 23：
Physiological and genetic characterization of two-component systems in Myxococcus / Chapter 24：
Detection and measurement of two-component systems that control dimorphism and virulence in fungi / Chapter 25：
Using Two-Component Systems and other Bacterial Regulatory Factors for the Fabrication of Synthetic Genetic Devices / Chapter 26：
Computational Analyses of Sequences and Sequence Alignments / Section I：
Comparative genomic and protein sequence analyses of a complex system controlling bacterial chemotaxis / Chapter 1：
Two component systems in microbial communities: Approaches and resources for analyzing metagenomic data sets / Chapter 2：
Alexandrine; Simon, Melvin I.; Crane, Brian; Crane, Alexandrine Crane, Alexandrine Crane, Brian R. Crane, Melvin I. Simon, Brian Crane, Michael I. Johnson
The PICM Chemical Scanning Method for Identifying Domain-Domain and Protein-Protein Interfaces: Applications to the Core Signaling Complex of E. coli Chemotaxis / Randal B. Bass ; Aaron S. Miller ; Susan L. Gloor ; Joseph J. Falke1：
Use of Site-Directed Cysteine and Disulfide Chemistry to Probe Protein Structure and Dynamics: Applications to Soluble and Transmembrane Receptors of Bacterial Chemotaxis / Scott L. Butler ; Stephen A. Chervitz2：
Measuring Distances by Pulsed Dipolar ESR Spectroscopy: Spin-Labeled Histidine Kinases / Peter P. Borbat ; Jack H. Freed3：
Rigid Body Refinement of Protein Complexes with Long-Range Distance Restraints from Pulsed Dipolar ESR / Jaya Bhatnagar ; Brian R. Crane4：
TonB/TolA Amino-Terminal Domain Modeling / Kimberly L. Keller ; Kerry K. Brinkman ; Ray A. Larsen5：
Functional Dynamics of Response Regulators Using NMR Relaxation Techniques / Alexandra K. Gardino ; Dorothee Kern6：
The Design and Development of Tar-EnvZ Chimeric Receptors / Takeshi Yoshida ; Sangita Phadtare ; Masayori Inouye7：
Functional and Structural Characterization of EnvZ, an Osmosensing Histidine Kinase of E. coli / 8：
Light Modulation of Histidine-Kinase Activity in Bacterial Phytochromes Monitored by Size Exclusion Chromatography, Crosslinking, and Limited Proteolysis / Steffi Noack ; Tilman Lamparter9：
A Temperature-Sensing Histidine Kinase-Function, Genetics, and Membrane Topology / Yvonne Braun ; Angela V. Smirnova ; Helge Weingart ; Alexander Schenk ; Matthias S. Ullrich10：
The Regulation of Histidine Sensor Kinase Complexes by Quorum Sensing Signal Molecules / Matthew B. Neiditch ; Frederick M. Hughson11：
Reconstitution of Heterogeneous Systems / Section II：
Liposome-Mediated Assembly of Receptor Signaling Complexes / David J. Montefusco ; Abdalin E. Asinas ; Robert M. Weis12：
Analyzing Transmembrane Chemoreceptors Using In Vivo Disulfide Formation Between Introduced Cysteines / Wing-Cheung Lai ; Gerald L. Hazelbauer13：
Using Nanodiscs to Create Water-Soluble Transmembrane Chemoreceptors Inserted in Lipid Bilayers / Thomas Boldog ; Mingshan Li14：
Assays for CheC, FliY, and CheX as Representatives of Response Regulator Phosphatases / Travis J. Muff ; George W. Ordal15：
Genetic Dissection of Signaling Through the Rcs Phosphorelay / Nadim Majdalani ; Susan Gottesman16：
Intracellular Methods and Assays / Section III：
In Vivo Measurement by FRET of Pathway Activity in Bacterial Chemotaxis / Victor Sourjik ; I'd Vaknin ; Thomas S. Shimizu ; Howard C. Berg17：
In Vivo and In Vitro Analysis of the Rhodobacter sphaeroides Chemotaxis Signaling Complexes / Steven L. Porter ; George H. Wadhams ; Judith P. Armitage18：
In Vivo Crosslinking Methods for Analyzing the Assembly and Architecture of Chemoreceptor Arrays / Claudia A. Studdert ; John S. Parkinson19：
A "Bucket of Light" for Viewing Bacterial Colonies in Soft Agar / 20：
Phenotypic Suppression Methods for Analyzing Intra- and Inter-Molecular Signaling Interactions of Chemoreceptors / Peter Ames21：
Single-Cell Analysis of Gene Expression by Fluorescence Microscopy / Tim Miyashiro ; Mark Goulian22：
Genome-Wide Analyses of Two-Component Systems / Section IV：
Two-Component Systems of Mycobacterium tuberculosis-Structure-Based Approaches / Paul A. Tucker ; Elzbieta Nowak ; Jens Preben Morth23：
Transcriptomic Analysis of ArlRS Two-Component Signaling Regulon, a Global Regulator, in Staphylococcus aureus / Yinduo Ji ; Chuanxin Yu ; Xudong Liang24：
Global Analysis of Two-Component Gene Regulation in H. pylori by Mutation Analysis and Transcriptional Profiling / Biju Joseph ; Dagmar Beier25：
Phosphotransfer Profiling: Systematic Mapping of Two-Component Signal Transduction Pathways and Phosphorelays / Michael T. Laub ; Emanuele G. Biondi ; Jeffrey M. Skerker26：
Identification of Histidine Phosphorylations in Proteins Using Mass Spectrometry and Affinity-Based Techniques / Andrew R. S. Ross27：
Analysis of Gene Function using DNA Microarrays / Andrew P. Capaldi1：
An introduction to microarray data analysis and visualization / Gregg B. Whitworth2：
Genome-wide approaches to monitor pre-mRNA splicing / Maki Inada ; Jeff Pleiss3：
ChIP-Seq: Using high-throughput DNA sequencing for genome-wide identification of transcription factor binding sites / Philippe Lefrançois ; Wei Zheng ; Michael Snyder4：
Genome-wide mapping of nucleosomes in yeast / Oliver J. Rando5：
Genome-wide translational profiling by ribosome footprinting / Nicholas T. Ingolia6：
Systematic Genetic Analysis / Part II：
Synthetic Genetic Array (SGA) Analysis in Saccharomyces cerevisiae and Schizosaccharomyces pombe / Anastasia Baryshnikova ; Michael Costanzo ; Scott Dixon ; Franco J. Vizeacoumar ; Chad L. Myers ; Brenda Andrews ; Charles Boone7：
Making temperature-sensitive mutants / Shay Ben-Aroya ; Xuewen Pan ; Jef D. Boeke ; Philip Hieter8：
Quantitative Genetic Interaction Mapping using the E-MAP Approach / Sean R. Collins ; Assen Roguev ; Nevan J. Krogan9：
Exploring gene function and drug action using chemogenomic dosage assays / Elke Ericson ; Shawn Hoon ; Robert P. St. Onge ; Guri Giaever ; Corey Nislow10：
Proteomics / Part III：
Yeast Expression Proteomics by High-Resolution Mass Spectrometry / Tobias C. Walther ; Jesper V. Olsen ; Matthias Mann11：
High-quality binary interactome mapping / Matija Dreze ; Dario Monachello ; Claire Lurin ; Michael E. Cusick ; David E. Hill ; March Vidal ; Pascal Braun12：
Quantitative analysis of protein phosphorylation on a system-wide scale by mass spectrometry based proteomics / Bernd Bodenmiller ; Rudi Aebersold13：
A Toolkit of Protein-fragment Complementation Assays for Studying and Dissecting Large-scale and Dynamic Protein-Protein Interactions in Living Cells / Stephen W. Michnick ; Po-Hien Ear ; Christian Landry ; Mohan K. Malleshaiah ; Vincent Messier14：
Yeast Lipid Analysis and Quantification by Mass Spectrometry / Xue Li Guan ; Isabelle Riezman ; Markus R. Wenk ; Howard Riezman15：
Mass Spectrometry-Based Metabolomics of Yeast / Christopher A. Crutchfield ; Wenyun Lu ; Eugene Melamud ; Joshua D. Rabinowitz16：
Systems Analysis / Part IV：
Imaging single mRNA molecules in yeast / Hyun Youk ; Arjun Raj ; Alexander van Oudenaarden17：
Experimental evolution in yeast: a practical guide / Maitreya J. Dunham19：
Synthetic Biology / Part V：
Enhancing stress resistance and production phenotypes through transcriptome engineering / Felix H. Lam ; Franz S. Hartner ; Gerald R. Fink ; Gregory Stephanopoulos20：
Advances in Cytology/Biochemistry / Part VI：
Visualizing yeast chromosomes and nuclear architecture / Peter Meister ; Lutz R. Gehlen ; Elisa Varela ; Veronique Kalck ; Susan M. Gasser21：
Quantitative localization of chromosomal loci by immunofluorescence / Donna Garvey Brickner ; William Light ; Jason H. Brickner22：
Spinning Disk Confocal Microscopy of Yeast / Kurt Thorn23：
Correlative GFP-immunoelectron microscopy in yeast / Christopher Buser ; Kent McDonald24：
Analyzing P-bodies and stress granules in Saccharomyces cerevisiae / J Ross Buchan ; Tracy Nissan ; Roy Parker25：
Analyzing mRNA expression using single mRNA resolution fluorescent in situ hybridization / Daniel Zenklusen ; Robert H Singer26：
The Use of In Vitro Assays to Measure Endoplasmic Reticulum Associated Degradation (ERAD) / Jeffrey L. Brodsky27：
A protein transformation protocol for introducing yeast prion Particles into yeast / Motomasa Tanaka28：
Overexpression and Purification of Integral Membrane Proteins in Yeast / Franklin A. Hays ; Zygy Roe-Zurz ; Robert M. Stroud29：
Biochemical, cell biological and genetic assays to analyze amyloid and prion aggregation in yeast / Simon Alberti ; Randal Halfmann ; Susan Lindquist30：
Other Fungi / Part VII：
Genetics and Molecular Biology in Candida albicans / Hernday, A.D. ; Noble, S. M. ; Mitrovich, Q.M. ; Johnson, A.D31：
Molecular Genetics of Schizosaccharomyces pombe / Sarah A. Sabatinos ; Susan L. Forsburg32：
Applying Genetics and Molecular Biology to the Study of the Human Pathogen Cryptococcus neoformans / Cheryl D. Chun ; Hiten D. Madhani33：
The Fungal Genome Initiative and lessons learned from genome sequencing / Christina A. Cuomo ; Bruce W. Birren34：
Ultradian metabolic cycles in yeast / Benjamin Tu35：
Functional Genomics / Part I：
Analysis of Gene Function using DNA Microarrays / Andrew P. Capaldi1：
An introduction to microarray data analysis and visualization / Gregg B. Whitworth2：
Measurement and Analysis of Equilibrium Binding Titrations: A beginner's guide / Dorothy Beckett
Macromolecular Competition Titration Method: Accessing Thermodynamics of the Unmodified Macromolecule - Ligand Interactions Through Spectroscopic Titrations of Fluorescent Analogs / Wlodzimierz Bujalowski ; Maria J. Jezewska
Analysis of PKR-RNA interactions by sedimentation velocity / C. Jason Wong ; Katherine Launer-Felty ; James L. Cole
Structural and Thermodynamic Analysis of PDZ/Ligand Interactions / Tyson R. Shepherd ; Ernesto J. Fuentes
Thermodynamic analysis of metal ion-induced protein assembly / Andrew B. Herr ; Deborah G. Conrady
Thermodynamic Dissection of Colicin Interactions / Nicholas G Housden ; Colin Kleanthous
Energetics of Src homology domain interactions in receptor tyrosine kinase-mediated signaling / John E. Ladbury ; Stefan T. Arold
Structural and Functional Energetic Linkages in Allosteric Regulation of Muscle Pyruvate Kinase / J. Ching Lee ; Petr Herman
Analysis of Free Energy versus Temperature Curves in Protein Folding and Macromolecular Interactions / Vince J. LiCata ; Chin-Chi Liu
Application of the Sequential n-Step Kinetic Mechanism to Polypeptide Translocases / Aaron L. Lucius ; Justin M. Miller ; Burki Rajendar
A Coupled Equilibrium Approach to Study Nucleosome Thermodynamics / Andrew J. Andrews ; Karolin Luger
Quantitative methods for measuring DNA flexibility in vitro and in vivo / Justin P. Peters ; Nicole Becker ; Emily M. Rueter ; Zeljko Bajzer ; Jason D. Kahna ; L. James Maher
A Thermodynamic Approach for the Targeting of Nucleic Acid Structures Using Their Complementary Single Strands / Hui-Ting Lee ; Caroline Carr ; Hollie Siebler ; Lela Waters ; Irine Khutsishvili ; Fany Iseka ; Brian Domack ; Chris M. Olsen ; Luis Marky
Thermodynamics of Biological Processes / Hernan G. Garcia ; Jane Kondev ; Nigel Orme ; Julie Theriot ; Rob Phillips
Protein stability in the presence of co-solutes / Luis Marcelo F. Holthauzen ; Matthew Auton ; Mikhail Sineva ; Jorg Rosgen
Small Angle X-ray Scattering Studies of Peptide-Lipid Interactions using the Mouse Paneth Cell a-Defensin Cryptdin-4 / Abhijit Mishra ; Kenneth P. Tai ; Nathan W. Schmidt ; André J. Ouellette ; Gerard C. L. Wong
Synergy of molecular dynamics and isothermal titration calorimetry in studies of allostery / Rebecca Strawn ; Thomas Stockner ; Milan Melichercik ; Lihua Jin ; Wei-Feng Xue ; Jannette Carey ; Rüdiger Ettrich
Using tryptophan fluorescence to measure the stability of membrane proteins folded in liposomes / C. Preston Moon1 ; Karen G. Fleming
Non-B Conformations of CAG Repeats Using 2-aminopurine / Natalya N. Degtyareva ; Jeffrey T. Petty
Disulfide bond-mediated passenger domain stalling as a structural probe of autotransporter outer membrane secretion in vivo / Jonathan P. Renn ; Patricia L. Clark
Strategies for the Thermodynamic Characterization of Linked Binding/Local Folding Reactions Within the Native State: Application to the LID Domain of Adenylate Kinase from Escherichia coli / Travis P. Schrank ; W. Austin Elam ; Jing Li ; Vincent J. Hilser
Measurement and Analysis of Equilibrium Binding Titrations: A beginner's guide / Dorothy Beckett
Macromolecular Competition Titration Method: Accessing Thermodynamics of the Unmodified Macromolecule - Ligand Interactions Through Spectroscopic Titrations of Fluorescent Analogs / Wlodzimierz Bujalowski ; Maria J. Jezewska
Analysis of PKR-RNA interactions by sedimentation velocity / C. Jason Wong ; Katherine Launer-Felty ; James L. Cole
Predicting Fluorescence Lifetimes and Spectra of Biopolymers / Patrik R. Callis
Modeling of Regulatory Networks: Theory and Applications in the study of the Drosophila Circadian Clock / Elizabeth Y. Scribner ; Hassan M. Fathallah-Shaykh
Strategies for articulated multibody-based adaptive coarse grain simulation of RNA / Mohammad Poursina ; Kishor D. Bhalerao ; Samuel C. Flores ; Kurt S. Anderson ; Alain Laederach
Modeling Loop Entropy / Gregory S. Chirikjian
Inferring Functional Relationships and Causal Network Structure from Gene Expression Profiles / Radhakrishnan Nagarajan
Numerical solution of the chemical master equation: uniqueness and stability of the stationary distribution for chemical networks, and mRNA bursting in a gene network with negative feedback regulation / E. S. Zeron ; M. Santill_an
How molecular should your molecular model be? On the level of molecular detail required to simulate biological networks in systems and synthetic biology / Didier Gonze ; Wassim Abou-Jaoud´e ; Adama Ouattara ; Jos´e Halloy
Computational modelling of biological pathways by executable biology / Maria Luisa Guerriero John K. Heath
Computing Molecular Fluctuations in Biochemical Reaction Systems Based on A Mechanistic, Statisitical Theory of Irreversible Processes / Don Kulasiri
Probing the input-output behaviour of biochemical and genetic systems: system identification methods from control theory / David McMillen ; Brian Ingalls ; Jordan Ang
Biochemical pathway modelling tools for drug target detection in cancer and other complex diseases / Alberto Marin-Sanguino ; Shailendra K. Gupta ; Eberhard O. Voit ; Julio Vera
Deterministic and Stochastic Simulation and Analysis of Biochemical Reaction Networks: The Lactose Operon Example / Necmettin Yildirim ; Caner Kazanci F.
Multivariate Neighborhood Sample Entropy: A method for data reduction and prediction of complex data / Joshua S. Richman MD, PhD
Scaling differences of heartbeat excursions between wake and sleep periods / L. Guzm´an-Vargas ; I. Reyes-Ram´irez ; R. Hern´andez-P´erez ; F. Angulo-Brown
Changepoint analysis for single-molecule polarized totalinternal reflection fluorescence microscopy experiments / John F. Beausang ; Yale E. Goldman ; Philip C. Nelson
Inferring mechanisms from dose-response curves / Carson C. Chow ; Karen M. Ong ; Edward J. Dougherty ; S. Stoney Simons, Jr
Spatial Aspects in Biological System Simulations / Haluk Resat ; Michelle N. Costa ; Harish Shankaran
Computational Approaches to Modeling Viral Structure and Assembly / Stephen C. Harvey ; Anton S. Petrov ; Batsal Devkota ; Mustafa Burak Boz
An Object-Oriented Software Suite for the Simulation and Design of Macromolecules / Andrew Leaver-Fay ; Michael Tyka ; Steven M. Lewis ; Oliver F. Lange ; James Thompson ; Ron Jacak ; Kristian Kaufman ; P. Douglas Renfrew ; Colin A. Smith ; Will Sheffler ; Ian W. Davis ; Seth Cooper ; Adrien Treuille ; Daniel J. Mandell ; Florian Richter ; Yih-En Andrew Ban ; Sarel J. Fleishman ; Jacob E. Corn ; David E. Kim ; Sergey Lyskov ; Monica Berrondo ; Stuart Mentzer ; Zoran Popovic ; James J. Havranek ; John Karanicolas ; Rhiju Das ; Jens Meiler ; Tanja Kortemme ; Jeffrey J. Gray ; Brian Kuhlman1 ; David Baker ; Philip Bradley
Computational Design of Intermolecular Stability and Specificity in Protein Self-Assembly / Vikas Nanda ; Sohail Zahid ; Fei Xu ; Daniel Levine
Predicting Fluorescence Lifetimes and Spectra of Biopolymers / Patrik R. Callis
Modeling of Regulatory Networks: Theory and Applications in the study of the Drosophila Circadian Clock / Elizabeth Y. Scribner ; Hassan M. Fathallah-Shaykh
Strategies for articulated multibody-based adaptive coarse grain simulation of RNA / Mohammad Poursina ; Kishor D. Bhalerao ; Samuel C. Flores ; Kurt S. Anderson ; Alain Laederach
Identification and characterization of Steroidogenic Factor-1 inverse agonists / Mabrouka Doghman ; Franck Madoux ; Peter Hodder ; Enzo Lalli
Assessment of inverse agonism for the angiotensin II type 1 receptor / Hiroshi Akazawa ; Noritaka Yasuda ; Shin-ichiro Miura ; Issei Komuro
Measurement of inverse agonism in BARs / Carlos Alberto Taira
Inverse agonism of antidepressants: in vitro and in vivo studies / Joel Bockaert
Differential inverse agonism at the human muscarinic M3 receptor / Paola Casarosa
Ghrelin Receptor: High Constitutive Activity and Methods for Developing Inverse Agonists / Constance Chollet
Constitutive activity and inverse agonism at the a1a and a1b adrenergic receptor subtypes / Susanna. Cotecchia
Measurement of inverse agonism of the cannabinoid receptor / Tung Fong
Constitutively Active Thyrotropin (TSH) and Thyrotropin-Releasing Hormone (TRH) Receptors and Their Inverse Agonists / Marvin C. Gershengorn
Inverse retinoid agonists and neutral antagonists / Hinrich Gronemeyer
y-Aminobutyric Acid Type A (GABAA) Receptor Subtype Inverse Agonists as Therapeutic Agents in Cognition / Gabriella Guerrini ; Ciciani Giovanna
Assays for inverse agonists in the visual system / Masahiro Kono
Receptor-driven identification of novel human A3 adenosine receptor antagonists as potential therapeutic agents / Silvia Paoletta ; Stephanie Federico ; Giampiero Spalluto ; Stefano Moro
Inverse agonists of steroidogenic factor SF-1 / Fabrice Piu ; Andria L. Del Tredici
Methods to Measure G Protein Coupled Receptor Activity for the Identification of Inverse Agonists / Gabriel Barreda-Gómez ; M.Teresa Giralt ; Rafael Rodríguez-Puertas
Novel Strategies and Techniques for Constitutive Activity and Inverse Agonism / Section II：
Use of Pharmacoperones to Reveal GPCR Structural Components Associated with Receptor Activation and Trafficking / Jo Ann Janovick ; P. Michael Conn
Application of large-scale transiently transfected cells to functional assays of ion channels and other proteins / Jun Chen ; Sujatha Gopalakrishnan ; Marc R Lake ; Bruce R Bianchi
Quantification of RNA Editing of the Serotonin 2C Receptor (5-HT2CR) Ex Vivo / Maria Fe Lanfranco ; Noelle C. Anastasio ; Patricia K. Seitz ; Kathryn A. Cunningham
Strategies for isolating constitutively-active and dominant-negative pheromone receptor mutants in yeast / Mercedes Dosil ; James B. Konopka
Development of a GPR23 Cell-based ß-Lactamase Reporter Assay / Paul H. Lee ; Bonnie J. Hanson
Computational Modeling of Constitutively Active Mutants of GPCRs: C5a Receptor / Gregory V. Nikiforovich ; Thomas J. Baranski
TSH receptor monoclonal antibodies with agonist, antagonist and inverse agonist activities / Jane Sanders ; Ricardo Núñez Miguel ; Jadwiga Furmaniak ; Bernard Rees Smith
Current standards, variations and pitfalls for the determination of constitutive TSHR activity in vitro / Sandra Mueller ; Holger Jaeschke ; Ralf Paschke
Towards the Rational Design of Constitutively Active KCa3.1 Mutant Channels / Line Garneau ; Hélène Klein ; Lucie Parent ; Rémy Sauvé
Fusion proteins as model systems for the analysis of constitutive GPCR activity / Erich H. Schneider ; Roland Seifert
Screening for Novel Constitutively Active CXCR2 Mutants and Their Cellular Effects / Giljun Park ; Tom Masi ; Chang K. Choi ; Heejung Kim ; Jeffrey M. Becker ; Tim E. Sparer
A method for parallel solid-phase synthesis of iodinated analogues of the cannabinoid receptor type I (CB1) inverse agonist rimonabant / Alan C. Spivey ; Chih-Chung Tseng
Coexpression systems as models for the analysis of constitutive GPCR activity
Modelling and simulation of inverse agonism dynamics / Lloyd J. Bridge
Design and Use of Constitutively Active STAT5 Constructs / Michael A. Farrar
In vitro and in vivo assays of protein kinase CK2 activity / Renaud Prudent ; Céline F. Sautel ; Virginie Moucadel ; Béatrice Laudet ; Odilhe Filhol ; Claude Cochet
Identification and characterization of Steroidogenic Factor-1 inverse agonists / Mabrouka Doghman ; Franck Madoux ; Peter Hodder ; Enzo Lalli
Assessment of inverse agonism for the angiotensin II type 1 receptor / Hiroshi Akazawa ; Noritaka Yasuda ; Shin-ichiro Miura ; Issei Komuro
Measurement of inverse agonism in BARs / Carlos Alberto Taira
Identification and Measurement of Constitutive Activity / Section I：
Constitutive activity at the cannabinoid CB1 receptor and behavioral responses / Katherine E. Hanlon ; Todd W. Vanderah
Detecting constitutive activity and protean agonism at the cannabinoid-2 receptor / Massimiliano Beltramo ; Rossella Brusa ; Isabella Mancini ; Paola Scandroglio ; Massimiliano. Beltramo
Modulation of constitutive activity of the ghrelin receptor by use of pharmacological tools as well as mutagenesis / Birgitte Holst ; Jacek Mokrosinski
Assessment of constitutive activity and internalization of GPR54 (Kiss1-r) / Macarena Pampillo ; Andy Videsh Babwah
Assessment of Constitutive Activity in EP Prostanoid Receptors / Hiromichi Fujino ; Toshihiko Murayama ; John W. Regan
a1D-Adrenergic receptors: constitutive activity and reduced expression at the plasma membrane / J Adolfo Garcia-Sáinz ; M. Teresa Romero-Ávila ; Luz del Carmen Medina
Constitutive activity of the histamine H1 receptor / Saskia Nijmeijer ; Rob Leurs ; Henry F Vischer
Assessment of homologous internalization of constitutively active N111G mutant of AT1 receptor / Mohiuddin Ahmed Bhuiyan ; Takafumi Nagatomo
Methods to detect cell surface expression and constitutive activity of GPR6 / Balakrishna M. Prasad ; Bettye Hollins ; Nevin A. Lambert
ß3-adrenoceptor agonists and (antagonists as) inverse agonists: history, perspective, constitutive activity and stereospecific binding / Maria Grazia Perrone ; Antonio Scilimati
Constitutive Activity of the Lutropin Receptor and its Allosteric Modulation by Receptor Heterodimerization / Deborah L. Segaloff
Assessing Constitutive Activity of Extracellular Calcium-Sensing Receptors in Vitro and in Bone / Wenhan Chang ; Melita Dvorak ; Dolores Shoback
Constitutive Activity of Neural Melanocortin Receptors / Ya-Xiong Tao ; Hui Huang ; Zhi-Qiang Wang ; Fan Yang ; Jessica N. Williams ; Gregory V. Nikiforovich
Measurement of Constitutive Activity of BMP Type I receptors / Peter ten Dijke
Probing the CA among dopamine D1 and D5 receptors and their mutants / Bianca Plouffe ; Jean-Philippe D'Aoust ; Vincent Laquerre ; Binhui Liang ; Mario Tiberi
Identification of Gain-of-Function Variants of the Human Prolactin Receptor / Vincent Goffin ; Roman L. Bogorad ; Philippe Touraine
Investigations of Activated ACVR1/ALK2, a Bone Morphogenetic Protein (BMP) Type I Receptor that Causes Fibrodysplasia Ossificans Progressiva / Frederick. Kaplan ; Petra Seemann ; Julia Haupt ; Meiqi Xu ; Vitali Lounev ; Mary Mullins ; Eileen M. Shore
Identification and evaluation of constitutively active thyroid stimulating hormone receptor mutations / Joaquin Lado-Abeal ; Leah R. Quisenberry ; Isabel Castro-Piedras
Assessment of constitutive activity of a G protein-coupled receptor, Cpr2, in Cryptococcus neoformans by heterologous and homologous methods / Chaoyang Xue ; Yina Wang ; Yen-Ping Hsueh
In vitro and in vivo assessment of mu opioid receptor constitutive activity / Edward J. Bilsky ; Denise Giuvelis ; Melissa D. Osborn ; Christina M. Dersch ; Heng Xu ; Richard B. Rothman
Constitutively active µ-opioid methods / Mark. Connor ; John Traynor
Protein Kinase Ck2 is a Constitutively-Active Enzyme That Promotes Cell Survival: Strategies To Identify Ck2 Substrates and Manipulate Its Activity In Mammalian Cells / Jacob P. Turowec ; James S. Duncan ; Ashley C. French ; Laszlo Gyenis ; Nicole A. St. Denis ; Greg J. Vilk ; David W. Litchfield
Assessment of Ck2 Constitutive Activity In Cancer Cells / Maria Ruzzene ; Giovanni Di Maira ; Kendra Tosoni ; Lorenzo A. Pinna
Structural basis of the constitutive activity of protein kinase CK2 / Birgitte B. Olsen ; Barbara Guerra ; Karsten Niefind ; Olar-Georg Issinger font error
Measuring the Constitutive Activation of c-Jun N-terminal Kinase Isoforms / Ryan T. Nitta ; Shawn S. Badal ; Albert J. Wong
Measurement of constitutive MAPK and PI3K/AKT signaling activity in human cancer cell lines / Keiran S.M. Smalley
Constitutive activity of GPR40/FFA1: Intrinsic or assay dependent? / Leigh A Stoddart ; Graeme Milligan
Constitutive activity of TRP channels: methods for measuring the activity and its outcome / Baruch Minke
Measurement of Orexin (Hypocretin) and Substance P Effects On Constitutively Active Inward Rectifier K+ Channels in Brain Neurons / Yasuko Nakajima ; Shigehiro Nakajima
Characterization of G protein receptor kinase 4 and measuring its constitutive activity in vivo / Bradley T. Andresen
Voltage-clamp based methods for the detection of constitutively active acetylcholine-gated IK,ACh channels in the diseased heart / Niels Voigt ; Samy Makary ; Stanley Nattel ; Dobromir Dobrev
Assaying Wave and WASH complex constitutive activities towards the Arp2/3 complex / Emmanuel Derivery ; Alexis Gautreau
Identification and Measurement of Constitutive Activity / Section I：
Constitutive activity at the cannabinoid CB1 receptor and behavioral responses / Katherine E. Hanlon ; Todd W. Vanderah
Detecting constitutive activity and protean agonism at the cannabinoid-2 receptor / Massimiliano Beltramo ; Rossella Brusa ; Isabella Mancini ; Paola Scandroglio ; Massimiliano. Beltramo
John N. Abelson, Sidney P. Colowick, Yusuf A. Hannun, Alfred H. Merrill, Alfred Harrison Merrill, Jr., Alfred H. Merrill, Melvin I. Simon, A. Hannum Yusuf, Michael I. Johnson, Masayasu N. Kojima
Assays for the Synthesis of Sphingomyelin (Ceramide Phosphocholine) and Ceramide Phosphoethanolamine / J.A. Shayman ; A. Abe
Glucosylceramide Synthase: Assay and Properties / P. Marks ; Y. Kamisaka ; R.E. Pagano
Methods for Studying Glucosylceramide Synthase / H. Sprong ; G. van Meer ; P. van der Sluijs
UDP-Galactose: Ceramide Galactosyltransferase / S. Chatterjee
Assay of Lactosylceramide Synthase and Comments on Its Potential Role in Signal Transduction / G. Pohlentz ; C. Kaes ; K. Sandhoff
In Vitro Assays for Enzymes of Ganglioside Synthesis / F.B. Jungalwala ; P. Chatturvedi ; D.S. Newburg ; M.R. Natowicz
Analyses of Sulfatides and Enzymes of Sulfatide Metabolism
1-O-Acyleceramide Synthase / T-C. Lee
N-Acetylation of Shingosine by Platelet-Activating Factor: Sphingosine Transacetylase / A.S. Fischl ; Y. Liu ; A. Browdy ; A.E. Cremesti
Inositolphosphoryl Ceramide Synthase from Yeast / D.V. Lynch
Enzymes of Shingolipid Metabolism in Plants
Turnover / S. Lansmann ; O. Bartelsen
Purification and Characterization of Recombinant Human Acid Sphingomyelinase / B. Liu ; Y.A. Hannun
Purification of Rat Brain Membrane Neutral Sphingomyelinase / B.Liu
Sphingolmyelinase Assay using Radiolabeled Substrates / A.G. Barbone ; A.C. Jackson ; D.M. Ritchie ; D.C. Argentieri
Robotic Assay of Sphingomyelinase Activity for High-Throughput Screening / D.F. Hassler ; R. M. Laethem ; G.K. Smith
A High Through-put Sphingomyelinase Assay / R.T. Dobrowsky ; V.G. Rao
Analysis of Sphingomyelin Hydrolosis in Caveolar Membranes / M.N. Nikolova-Karakashian
Ceramidases / T.Linke ; S. Lansman
Purification of Acid Ceramidase from Human Placenta / S. Bajjalieh ; R. Batchelor
Ceramide Kinase / A.Olivera ; K.D. Barlow ; S. Spiegel
Assaying Sphingosine Kinase Activity / L.M. Obeid
Sphingosine 1-Phosphate Phosphatase / D.N. Brindley ; J. Xu ; R. Jasinska ; D.W. Waggoner
Analysis of Ceramide 1-Phosphate and Sphingosine 1-Phosphate Phosphatase Activities / P. Van Veldhover
Sphingosine 1-Phosphate Lyase / W. Bierfreund ; T. Kolter
Sphingolipid Hydrolases and Activator Proteins / R-D. Duan ; A. Nilsson
Sphingolipid Hydrolyzing Enzymes in the Gastrointestinal Tract / M. Basu ; P. Kelly ; M. Girzadas ; Z. Li ; S. Basu
Properties of Animal Ceramide Glycanases / M. Ito ; K. Kita ; T. Kurita ; N. Sueyoshi ; H. Izu
Enzymatic N-Deacylation of Shingolipids
Genetic Approaches / S. Ichikawa ; Y. Hirabayashi
Genetic Approaches for Studies of Glycolipid Synthetic Enzymes / N.Chung
Use of Yeast as a Model System for Studies of Sphingolipid Metabolism and Signaling
Inhibitors and Sphingolipid Biosynthesis / S.M. Mandala ; G. H. Harris
Isolation and Characterization of Novel Inhibitors of Sphingolipid Synthesis: Austalifungin, Viridiofungins, Rustmicin, and Khafrefungin / R.T. Riley ; R.D. Plattner
Fermentation, Partial Purification, and Use of Serine Palmitoyltransferase Inhibitors from Isaria (Cordyceps) sinclairii / F. Meredith
Isolation and Characterization of Fumonisins / L. Lee ; L. Shu
John N. Abelson, Sidney P. Colowick, Yusuf A. Hannun, Alfred Harrison Merrill, Jr., Alfred H. Merrill, Melvin I. Simon, Michael I. Johnson, Masayasu N. Kojima
Assays for the Synthesis of Sphingomyelin (Ceramide Phosphocholine) and Ceramide Phosphoethanolamine / J.A. Shayman ; A. Abe
Glucosylceramide Synthase: Assay and Properties / P. Marks ; Y. Kamisaka ; R.E. Pagano
Methods for Studying Glucosylceramide Synthase / H. Sprong ; G. van Meer ; P. van der Sluijs
UDP-Galactose: Ceramide Galactosyltransferase / S. Chatterjee
Assay of Lactosylceramide Synthase and Comments on Its Potential Role in Signal Transduction / G. Pohlentz ; C. Kaes ; K. Sandhoff
In Vitro Assays for Enzymes of Ganglioside Synthesis / F.B. Jungalwala ; P. Chatturvedi ; D.S. Newburg ; M.R. Natowicz
Analyses of Sulfatides and Enzymes of Sulfatide Metabolism
1-O-Acyleceramide Synthase / T-C. Lee
N-Acetylation of Shingosine by Platelet-Activating Factor: Sphingosine Transacetylase / A.S. Fischl ; Y. Liu ; A. Browdy ; A.E. Cremesti
Inositolphosphoryl Ceramide Synthase from Yeast / D.V. Lynch
Enzymes of Shingolipid Metabolism in Plants
Turnover / S. Lansmann ; O. Bartelsen
Purification and Characterization of Recombinant Human Acid Sphingomyelinase / B. Liu ; Y.A. Hannun
Purification of Rat Brain Membrane Neutral Sphingomyelinase / B.Liu
Sphingolmyelinase Assay using Radiolabeled Substrates / A.G. Barbone ; A.C. Jackson ; D.M. Ritchie ; D.C. Argentieri
Robotic Assay of Sphingomyelinase Activity for High-Throughput Screening / D.F. Hassler ; R. M. Laethem ; G.K. Smith
A High Through-put Sphingomyelinase Assay / R.T. Dobrowsky ; V.G. Rao
Analysis of Sphingomyelin Hydrolosis in Caveolar Membranes / M.N. Nikolova-Karakashian
Ceramidases / T.Linke ; S. Lansman
Purification of Acid Ceramidase from Human Placenta / S. Bajjalieh ; R. Batchelor
Ceramide Kinase / A.Olivera ; K.D. Barlow ; S. Spiegel
Assaying Sphingosine Kinase Activity / L.M. Obeid
Sphingosine 1-Phosphate Phosphatase / D.N. Brindley ; J. Xu ; R. Jasinska ; D.W. Waggoner
Analysis of Ceramide 1-Phosphate and Sphingosine 1-Phosphate Phosphatase Activities / P. Van Veldhover
Sphingosine 1-Phosphate Lyase / W. Bierfreund ; T. Kolter
Sphingolipid Hydrolases and Activator Proteins / R-D. Duan ; A. Nilsson
Sphingolipid Hydrolyzing Enzymes in the Gastrointestinal Tract / M. Basu ; P. Kelly ; M. Girzadas ; Z. Li ; S. Basu
Properties of Animal Ceramide Glycanases / M. Ito ; K. Kita ; T. Kurita ; N. Sueyoshi ; H. Izu
Enzymatic N-Deacylation of Shingolipids
Genetic Approaches / S. Ichikawa ; Y. Hirabayashi
Genetic Approaches for Studies of Glycolipid Synthetic Enzymes / N.Chung
Use of Yeast as a Model System for Studies of Sphingolipid Metabolism and Signaling
Inhibitors and Sphingolipid Biosynthesis / S.M. Mandala ; G. H. Harris
Isolation and Characterization of Novel Inhibitors of Sphingolipid Synthesis: Austalifungin, Viridiofungins, Rustmicin, and Khafrefungin / R.T. Riley ; R.D. Plattner
Fermentation, Partial Purification, and Use of Serine Palmitoyltransferase Inhibitors from Isaria (Cordyceps) sinclairii / F. Meredith
Isolation and Characterization of Fumonisins / L. Lee ; L. Shu
Progress in Antisense Technology: The End of the Beginning / M.I. Phillips ; Y.C. Zhang
Basic Prinicples of Using Antisense Oligonucleotides in Vivo / T.E. Saison-Behmoaras ; S. Dheur
Polyethyleneimine-Mediated Transfection to Improve Antisense Activity of 3'-Capped Phosphodiester Oligonucleotides / J.-C. Franccedil;ois ; J. Lacoste ; L. Lacroix ; J.-L. Mergny
Design of Antisense and Triplex-Forming Oligonuceotides / R.V. Giles ; D.G. Spiller ; D.M. Tidd
Chimeric Oligodeoxynucleotide Analogs: Chemical Synthesis, Purification, and Molecular and Cellular Biology Protocols / P. Iversen ; C. Ghosh ; D. Stein ; D. Weller
Evaluation of Antisense Mechanisms of Action / P.-M. Lledo ; D. Desmaisons ; A. Carleton ; J.-D. Vincent
Physiology and Molecular Biology Brought to Single-Cell Level / P.E. Nielsen
Antisense Properties of Peptide Nucleic Acid / M.W. Reed ; D. Milesi ; I. Kutyavin ; E.A. Lukhtanov ; V.V. Gorn
Synthesis of Oligonucleotide Conjugates in Anhydrous Dimethyl Sulfoxide / P.A. Morcos
Gene Switching: Analyzing Broad Range of Mutations Using Steric Block / I. Robbins ; B. Lebleu
Vesicular Stomatitis Virus as Model System for Studies of Antisense Oligonucleotide Translation Arrest / Y.S. Sanghvi ; R.R. Deshmukh ; D.L. Cole
Purification of Antisense Oligonuceleotides / B.R. Shaw ; D. Sergueev ; K. He ; K. Porter ; J. Summers ; Z. Sergueeva ; V. Rait
Boranophosphate Backbone: A Mimic of Phosphodiesters, Phosphorothioates, and Methyl Phosphonates / J. Piulats ; G. Tarrzsoacute;n ; D. Bellido ; R. Eritja ; S. Vilaroacute;
Intracellular Distribution of Digoxigenin-Labled Phosphorothioate Oligonucleotides / E. Uhlmann ; A. Peyman ; A. Ryte ; A. Schmidt ; E. Buddecke
Use of Minimally Modified Antisense Oligonucleotides for Specific Inhibition of Gene Expression
Methods of Delivery / C.A. Stein ; L. Benimetskaya ; J. Tonkinson
Determination of Cellular Internalization of Fluorescent Oligonucleotides / P.O.P. Ts'o ; R.J. Duff ; S.F. Deamond ; C. Roby ; Y. Zhou
Lipid-Based Formulations of Antisense Oligonucleotides for Systemic Delivery Applications / R.L. Juliano ; J. Hughes ; A. Astriab ; H. Yoo ; S. Alahari ; E. Liang
In Vitro Transport and Delivery of Antisense Oligonucleotides / A. Matsuno ; T. Nagashima ; H. Katayama ; A. Tamura
In Vitro and in Vivo Delivery of Antisense Oligodeoxynucleotides Using Lipfection: Application of Antisense Technique to Growth Suppression of Experimental Glioma / A.M. Tari
Preparation and Application of Liposome-Incorporated Oligodeoxynucleotides / S.A. Williams ; J.S. Buzby
Cell-Specific Optimization of Phosphorothiate Antisense Oligodeoxynucelotide Delivery by Cationic Lipids
RNA Studies / J.J. Rossi ; D. Castanotto ; M. Scherr
Intracellular Expression and Function of Antisense Catalytic RNAs / J.M. Dagle ; D.L. Weeks
Selective Degradation of Targeted mRNAs Using Partially Modified Oligonucleotides / G.L. Eliceiri
Reversible Depletion of Specific RNAs by Antisense Oligodeoxynucelotide-Targeted Degradation in Frog Oocytes / C. Guerrier-Takada
General Methods / S.T. Crooke
Progress in Antisense Technology: The End of the Beginning / M.I. Phillips ; Y.C. Zhang
Basic Prinicples of Using Antisense Oligonucleotides in Vivo / T.E. Saison-Behmoaras ; S. Dheur
Antisense Receptor Targets / J. Garz=n ; I. de Antonio ; P. Sfnchez-Blfzquez
In vivo Modulation of G-proteins and Opioid Receptor Function by Antisense Oligodeoxynucleotides / J. Karle ; M. Nielsen
Targeting Brain GABA-A Receptors with Antisense Oligonucleotides: Implications for Epilepsy / D. Mohuezy ; X. Tang ; M.I. Phillips
Delivery of Antisense DNA by Vectors for Prolonged Effects in Vitro and in Vivo / G.W. Pasternak ; Y.-X. Pan
Antisense Mappling: Assessing the Functional Significance of Genes and Splice Variants / E.S.J. Robinson ; A.L. Hudson
In Vivo and in Vitro Effects of Antisense on 2-Adrenoceptor Expression / J.G. Hensler ; J.M. Scalzitti
Design and Efficacy of a Seritonin2A Receptor Antisense Oligodeoxynucleotide / M. Beinfeld ; D. Vishnuvardham
The Use of Expression of Antisense mRNA for the Enzymes PC1 and PC2 in Prohormone Processing
Antisense Neuroscience Targets / W.C. Broaddus ; S.S. Prabhu ; S. Wu-Pong ; G.T. Gillies ; H. Fillmore
Design and Testing of Antisense Oligonucleotide Strategies for the Central Nervous System / N.J. Buckley ; Fe.C. Abogadie ; D.A. Brown ; M. Dayrell ; M.P. Caulfield ; P. Delmas ; J.E. Haley
Use of Antisense Expression Plasmids to Attenuate G-Expression in Primary Neurons / S. Catsicas ; F. Hallb__k ; M. Catsicas ; J.K. Staple ; G. Grenningloh
Gene Functional Analysis in the Nervous System / S. Peng Ho ; D.H.O. Britton ; Y. Bao ; and M.S. Scully
RNA Mapping: Selection of Potent Oligonucleotide Sequences for the Antisense Experiment / P.S. Kalra ; M.G. Dube ; S.P. Kalra
Effects of Centrality Administered Antisense Oligodeoxynucleotides on Feeding Behavior and Hormone Secretion / J. Lai ; J.C. Hunter ; M.H. Ossipov ; F. Porreca
Blockade of Neuropathic Pain by Antisense Targeting of TTX-Resistant Sodium Channels in Sensory Neurons / R. Mileusnic
Application of Antisense Approach for the Study of Cell Adhesion Molecules in the Central Nervous System / I.D. Neumann ; N. Toschi
Sequence Design and Practical Implementation of Antisense Oligonucleotides in Neuroendocrinology / T.S. Nowak, Jr. ; K. Yufu ; Y. Yaida
Localization of Oligonucleotides in Brain by in situ Hybridization / F. Gremo ; V. Sogos ; M. Curot ; M. Setzu ; I. Mussini ; M. Grazia Ennas
Use of Antisense Oligonucleotides in Human Neuronal and Astrocytic Cultures / W. Sommer ; M.O. Hebb ; M. Heilig
Pharmacokinetic Properties of Oligonucleotides in the Brain / W. Tischmeyer
Antisense Oligonucleotides--Preparation and in vivo Application to Rat Brain / S.G. Volsen ; R.C. Lambert ; Y. Maulet ; M.D. Waard ; S. Gillard ; P.J. Craig ; A. Feltz
The Application of Antisense Techniques to Characterize NEuronal Ion Channels in vitro
Antisense in Non-Neuronal Tissues / M. Juhaszova ; M.K. Slodzinski
Antisense Inhibition of the Na-Ca Exchanger / E.A.L. Biessen ; H. Vietsch ; E.T. Rump ; K. Fluiter ; M.K. Bijsterbosch ; Th.J.C. van Berkel
Targeted Delivery of Antisense Oligonucleotides to Parenchymal Liver Cell in vivo / D. Mercola ; F. Bost ; R. McKay ; N.M. Dean
Antisense Methods for the Discrimination of Phenotypic Properties of Closely Related Gene Products: The Jun Kinase Family / C.L. Cioffi ; B.P. Monia
Evaluation of the Biological Role of c-Jun N-terminal Kinase (NJK) using an Antisense Approach / M.B. Ganz
The Role of Antisense in Kidney Cells / D.L. Mattson
Use of Antisense Techniques in the Rate Renal Medulla / T. Ochiya ; M. Terada
Antisense Approaches to in vitro Organ Culture / E.S. Raveche ; G.A. Parker ; B. Peng ; M. He ; S. Gould-Fogerite ; C.-C. Chou
In vitro and in vivo Anti-Proliferative
Antisense Receptor Targets / J. Garz=n ; I. de Antonio ; P. Sfnchez-Blfzquez
In vivo Modulation of G-proteins and Opioid Receptor Function by Antisense Oligodeoxynucleotides / J. Karle ; M. Nielsen
Targeting Brain GABA-A Receptors with Antisense Oligonucleotides: Implications for Epilepsy / D. Mohuezy ; X. Tang ; M.I. Phillips
Daniel W; Abelson, John N.; Simon, Melvin I.; Celander, Daniel W. Celander, John N. Abelson, Daniel W. Celander, Daniel Walter Celander, Michael I. Johnson, Masayasu N. Kojima, Melvin I. Simon
Pathway of Allosteric Control as Revealed by Intermediate States of Hemoglobin / J.M. Holt ; G.K. Ackers
Probes of Energy Transduction in Enzyme Catalysis / Y. Huang ; D.W. Bolen
Macromolecules and Water: Probing with Osmotic Stress / V.A. Parsegian ; R.P. Rand ; D.C. Rau
Linkage of Protein Assembly to Protein-DNA Binding / I. Wong ; T.M. Lohman
Linkage at Steady State: Allosteric Transitions of Thrombin / E. Di Cera ; Q.D. Dang ; Y. Ayala ; A. Vindigni
Thermal Denaturation Methods in Study of Protein Folding / E. Freire
Kinetics of Lipid Membrane Phase Transitions: A Volume Perturbation Calorimeter Study / L. Chen ; R.L. Biltonen ; M.L. Johnson
Tight Binding Affinities Determined from the Thermodynamic Linkage to Protons by Titration Calorimetry / M.L. Doyle ; G. Louie ; P. Dal Monte ; T. Sokoloski
Calorimetric Methods for Interpreting Protein-Ligand Interactions / H.F. Fisher ; N. Singh
Extracting Thermodynamic Data from Equilibrium Melting Curves for Oligonucleotide Order-Disorder Transitions / K.J. Breslauer
Thermodynamics and Mutations in RNA-Protein Interactions / K.B. Hall ; J.K. Kranz
Melting Studies of RNA Unfolding and RNA-Ligand Interactions / D.E. Draper ; T.C. Gluick
Structural-Perturbation Approaches to Thermodynamics of Site-Specific Protein-DNA Interactions / L. Jen-Jacobson
Thermodynamic Parameters from Hydrogen Exchange Measurements / Y. Bai ; J.J. Englander ; L. Mayne ; J.S. Milne ; S.W. Englander
Application of Pressure to Biochemical Equilibria: The Other Thermodynamic Variable / C.A. Royer
Molecular Volume / L.M. Rellick ; W.J. Becktel
Hydrostatic and Osmotic Pressure as Tools to Study Macromolecular Recognition / C.R. Robinson ; S.G. Sligar
Sedimentation Equilibrium as Thermodynamic Tool / T.M. Laue
Footprint Phenotypes: Structural Models of DNA-Binding Proteins from Chemical Modification Analysis of DNA / J. Yang ; J. Carey
Low-Temperature Electrophoresis Methods / M. Perrella ; I. Denisov
Use of Multiple Spectroscopic Methods to Monitor Equilibrium Unfolding of Proteins / M.R. Eftink
Probing Structural and Physical Basis of Protein Energetics Linked to Protons and Salt / B. Garcia-Moreno
Evaluating Contribution of Hydrogen Bonding and Hydrophobic Bonding to Protein Folding / C.N. Pace
Analyzing Solvent Reorganization and Hydrophobicity / B. Lee
Simple Force Field for Study of Peptide and Protein Conformational Properties / T.P. Creamer ; G.D. Rose
Probes for Analysis of Stability of Different Variants of Aspartate Aminotransferase / M. Martinez-Carrion ; A. Artigues ; A. Berezov ; M.L. Bianconi ; A.M. Reyes ; A. Iriarte
John N. Abelson, Robert Adron Harris, Robert Allison Harris, Melvin I. Simon, John R. Sokatch, John Robert Sokatch, Michael I. Johnson, Masayasu N. Kojima
John N.; Simon, Melvin I.; Balch, W. E.; Der, Channing J.; Hall, Alan Abelson, W. E. Balch, William Edward Balch, Channing J. Der, Alan Hall, Michael I. Johnson, Masayasu N. Kojima, Melvin I. Simon
Purification, Posttranslational Modification, and in Vitro Regulation / Section I.：
Purification and Biochemical Characterization of TC10 / Cheryl L. Neudauer ; Ian G. Macara1.：
Expression and Purification of Rho/RhoGDI Complexes / Paul W. Read ; Robert K. Nakamoto2.：
Bacterial Expressed DH and DH/PH Domains / Kent L. Rossman ; Sharon L. Campbell3.：
Biochemical Analysis of Regulation of Vav, a Guanine-Nucleotide Exchange Factor for Rho Family of GTPases / Raymond Mosteller ; Jaewon Han ; Balaka Das ; Daniel Broek4.：
Activation of Rac1 by Human Tiam1 / Gideon Bollag ; Anne M. Crompton ; Danielle ; Peverly-Mitchell ; Gaston G. M. Habets ; Marc Symons5.：
Activation of Rho GEF Activity by G[alpha subscript 13] / Matthew J. Hart ; William Roscoe6.：
Purification and Characterization of Guanine Nucleotide Dissociation Stimulator Protein / Jon P. Hutchinson ; Katrin Rittinger ; John F. Eccleston7.：
Purification and Biochemical Activity of Salmonella Exchange Factor SopE / Andrea Friebel ; Wolf-Dietrich Hardt8.：
Stimulation of Rho GDI Release by ERM Proteins / Akiko Mammoto ; Kazuo Takahashi ; Takuya Sasaki ; Yoshimi Takai9.：
Expression and Activity of Human Prenylcysteine-Directed Carboxyl Methyltransferase / Edwin Choy ; Mark Philips10.：
Purification and Evaluation of Large Clostridial Cytotoxins That Inhibit Small GTPases of Rho and Ras Subfamilies / Michael Moos ; Christoph von Eichel-Streiber11.：
Rho GTPase-Activating Toxins: Cytotoxic Necrotizing Factors and Dermonecrotic Toxin / Gudula Schmidt ; Klaus Aktories12.：
Purification and Activity of GTPase Targets / Section II.：
Measurement of GTPase Effector Affinities / Danny Manor13.：
Purification and in Vitro Activity of Rho-Associated Kinase / Mutsuki Amano ; Yuko Fukata ; Hiroaki Shimokawa ; Kozo Kaibuchi14.：
Purification and in Vitro Activities of p21-Activated Kinases / Charles C. King ; Abina M. Reilly ; Ulla G. Knaus15.：
Stimulation of Phospholipase C-[beta subscript 2] by Rho GTPases / Daria Illenberger ; Ilona Stephan ; Peter Gierschik ; Frieder Schwald16.：
Regulation of Phospholipase D1 Activity by Rho GTPases / Michael A. Frohman ; Yasunori Kanaho ; Yue Zhang ; Andrew J. Morris17.：
In Vitro Interaction of Phosphoinositide-4-phosphate 5-Kinases with Rac / Kimberley Tolias ; Christopher L. Carpenter18.：
Activity and Regulation of p35/Cdk5 Kinase Complex / Margareta Nikolic ; Li-Huei Tsai19.：
Actin Assembly Mediated by Arp2/3 Complex and WASP Family Proteins / R. Dyche Mullins ; Laura M. Machesky20.：
In Vitro Actin Polymerization Using Polymorphonuclear Leukocyte Extracts / Sally H. Zigmond21.：
Analysis of Rho GTPase Function / Section III.：
Determination of GTP Loading on Rac and Cdc42 in Platelets and Fibroblasts / Anser C. Azim ; Kurt L. Barkalow ; John H. Hartwig22.：
Determination of GTP Loading on Rho / Xiang-Dong Ren ; Martin Alexander Schwartz23.：
Use and Properties of ROCK-Specific Inhibitor Y-27632 / Shuh Narumiya ; Toshimasa Ishizaki ; Masayoshi Uehata24.：
Inducible Membrane Recruitment of Small GTP-Binding Proteins by Rapamycin-Based System in Living Cells / Flavia Castellano ; Philippe Chavrier25.：
Expression of Rho GTPases Using Retroviral Vectors / Frits Michiels ; Rob A. van der Kammen ; Lennert Janssen ; Garry Nolan ; John G. Collard26.：
Expression of Rho GTPases Using Adenovirus Vectors / Daniel M. Sullivan ; Toren Finkel27.：
In Vivo Activity of Wild-Type and Mutant PAKs / Luraynne C. Sanders ; Gary M. Bokoch28.：
Single Cell Assays for Rac Activity / Laura J. Taylor ; Amy B. Walsh ; Patrick Hearing ; Dafna Bar-Sagi29.：
Effect of Rho GTPases on Na-H Exchanger in Mammalian Cells / Sheryl P. Denker ; Weihong Yan ; Diane L. Barber30.：
Actin Filament Assembly in Permeabilized Platelets / 31.：
Rho GTPases: Secretion and Actin Dynamics in Permeabilized Mast Cells / Mark R. Holt ; Anna Koffer32.：
Isolation and in Vitro Contraction of Stress Fibers / Kazuo Katoh ; Yumiko Kano ; Keigi Fujiwara33.：
Inhibition of Rho GTPases Using Protein Geranylgeranyltransferase I Inhibitors / Said M. Sebti ; Andrew D. Hamilton34.：
Imaging Spatiotemporal Dynamics of Rac Activation in Vivo with FLAIR / Chester E. Chamberlain ; Vadim S. Kraynov ; Klaus M. Hahn35.：
Biological Assays of Rho GTPase Function / Section IV.：
Rho-like GTPases in Tumor Cell Invasion / Eva E. Evers ; Jean P. ten Klooster36.：
Mouse Embryo Fibroblasts: A Genetic Model System for Studying Rho- and Ras-Dependent Cell Cycle Progression / Giovanna M. D'Abaco ; Michael F. Olson37.：
Analyses of Transforming Activity of Rho Family Activators / Patricia A. Solski ; Karon Abe ; Channing J. Der38.：
Rho GTPases and Cell Migration-Fibroblast Wound Healing / Catherine D. Nobes39.：
Rho GTPases and Cell Migration: Measurement of Macrophage Chemotaxis / Gareth E. Jones ; Anne J. Ridley ; Daniel Zicha40.：
Rho GTPases and Macrophage Phagocytosis / Jayesh C. Patel ; Alan Hall ; Emmanuelle Caron41.：
Rho GTPases and Axonal Growth Cone Collapse / Alyson E. Fournier ; Robert G. Kalb ; Stephen M. Strittmatter42.：
Study of in Situ Function of Cytoskeletal Proteins in Lamellipodia and Filopodia Using Microscale Chromophore-Assisted Laser Inactivation / Eric V. Wong ; Daniel G. Jay43.：
Modulation of Actin Cytoskeleton by Salmonella GTPase Activating Protein SptP / Jorge E. Galan ; Yixin Fu44.：
Gene Fusions as Reporters of Gene Expression in Prokaryotic Cells / Section II.：
A Practical Guide to the Construction and Use of lac Fusions in Escherichia coli / Nicholas J. Hand ; Thomas J. Silhavy2.：
Tagging Exported Proteins Using Escherichia coli Alkaline Phosphatase Gene Fusions / Colin Manoil3.：
Applications of Gene Fusions to Green Fluorescent Protein and Flow Cytometry to the Study of Bacterial Gene Expression in Host Cells / Raphael H. Valdivia ; Lalita Ramakrishnan4.：
IVET and RIVET: Use of Gene Fusions to Identity Bacterial Virulence Factors Specifically Induced in Host Tissues / James M. Slauch ; Andrew Camilli5.：
Identification of Exported Bacterial Proteins via Gene Fusions to Yersinia pseudotuberculosis Invasin / Micah J. Worley ; Fred Heffron6.：
Gene Fusions as Reporters of Gene Expression in Eukaryotic Cells / Section III.：
Use of Imidazoleglycerolphosphate Dehydratase (His3) as a Biological Reporter in Yeast / Joe Horecka ; George F. Sprague, Jr.7.：
Use of Fusions to Human Thymidine Kinase as Reporters of Gene Expression and Protein Stability in Saccharomyces cerevisiae / Sofie Reda Salama8.：
Use of Fusions to Thymidine Kinase / Christiaan Karreman9.：
In Vivo Analysis of lacZ Fusion Genes in Transgenic Drosophila melanogaster / Stephen Small10.：
Utility of the Secreted Placental Alkaline Phosphatase Reporter Enzyme / Bryan R. Cullen11.：
Fusions to Imidazopyrazinone-Type Luciferases and Aequorin as Reporters / Satoshi Inouye12.：
Novel Methods for Chemiluminescent Detection of Reporter Enzymes / Corinne E. M. Olesen ; Yu-Xin Yan ; Betty Liu ; Dina Martin ; Brian D'Eon ; Ray Judware ; Chris Martin ; John C. Voyta ; Irena Bronstein13.：
Fusions to Chloramphenicol Acetyltransferase as a Reporter / Clayton Bullock ; Cornelia Gorman14.：
Fusions to [beta]-Lactamase as a Reporter for Gene Expression in Live Mammalian Cells / Gregor Zlokarnik15.：
Functional Tags for Biochemical Purification / Section IV.：
Purification of Proteins Using Polyhistidine Affinity Tags / Joshua A. Bornhorst ; Joseph J. Falke16.：
Generating Fusions to Glutathione S-Transferase for Protein Studies / Donald B. Smith17.：
Use of the Strep-Tag and Streptavidin for Detection and Purification of Recombinant Proteins / Arne Skerra ; Thomas G. M. Schmidt18.：
Streptavidin-Containing Chimeric Proteins: Design and Production / Takeshi Sano ; Charles R. Cantor19.：
Fusions to Maltose-Binding Protein: Control of Folding and Solubility in Protein Purification / Deepali Sachdev ; John M. Chirgwin20.：
Thioredoxin as a Fusion Partner for Production of Soluble Recombinant Proteins in Escherichia coli / Edward R. LaVallie ; Zhijian Lu ; Elizabeth A. Diblasio-Smith ; Lisa A. Collins-Racie ; John M. McCoy21.：
Affinity Purification of Recombinant Proteins Fused to Calmodulin or to Calmodulin-Binding Peptides / Peter Vaillancourt ; Chao-Feng Zheng ; Danny Q. Hoang ; Lisa Breister22.：
The S Tag Fusion System for Protein Purification / Ronald T. Raines ; Mark McCormick ; Thomas R. Van Oosbree ; Robert C. Mierendorf23.：
Fusions to Self-Splicing Inteins for Protein Purification / Ming-Qun Xu ; Henry Paulus ; Shaorong Chong24.：
Fusion Proteins Containing Cellulose-Binding Domains / Jae-Seon Park ; Hae-Sun Shin ; Roy H. Doi25.：
Biotinylation of Proteins in Vivo and in Vitro Using Small Peptide Tags / Millard G. Cull ; Peter J. Schatz26.：
Biotinylation of Proteins in Vivo: A Useful Post-translational Modification for Protein Analysis / John E. Cronan, Jr. ; Kelynne E. Reed27.：
Hybrid Proteins for Detection and Production of Antigens and Antibodies / Section V.：
Methods for Generating Multivalent and Bispecific Antibody Fragments / Ian Tomlinson ; Philipp Holliger28.：
Design and Use of Phage Display Libraries for the Selection of Antibodies and Enzymes / Francesca Viti ; Fredrik Nilsson ; Salvatore Demartis ; Adrian Huber ; Dario Neri29.：
Use of an Lpp-OmpA Fusion Vehicle for Bacterial Surface Display / Charles F. Earhart30.：
Identification of Bacterial Class I Accessible Proteins by Disseminated Insertion of Class I Epitopes / Dolph Ellefson ; Adrianus W. M. van der Velden ; David Parker31.：
Bordetella pertussis Adenylate Cyclase Toxin: A Vehicle to Deliver CD8-Positive T-Cell Epitopes into Antigen-Presenting Cells / Pierre Guermonprez ; Catherine Fayolle ; Gouzel Karimova ; Agnes Ullmann ; Claude Leclerc ; Daniel Ladant32.：
Use of Anthrax Toxin Fusions to Stimulate Immune Responses / Christopher C. Zarozinski ; R. John Collier ; Michael N. Starnbach33.：
Use of Fusions to Viral Coat Proteins as Antigenic Carriers for Vaccine Development / Peter G. Stockley ; Robert A. Mastico34.：
Epitope Tagging: General Method for Tracking Recombinant Proteins / Christian E. Fritze ; Thomas R. Anderson1.：
Markers for Cytology, Analysis of Protein Trafficking, and Lineage Tracing / Section II.：
Alkaline Phosphatase Fusions of Ligands or Receptors as in Situ Probes for Staining of Cells, Tissues, and Embryos / John G. Flanagan ; Hawai-Jong Cheng ; David A. Feldheim ; Mitsuharu Hattori ; Qiang Lu ; Pierre Vanderhaeghen2.：
Chimeric Molecules Employing Horseradish Peroxidase as Reporter Enzyme for Protein Localization in the Electron Microscope / Colin Hopkins ; Adele Gibson ; Jane Stinchcombe ; Clare Futter3.：
Biochemical Analyses of Trafficking with Horseradish Peroxidase-Tagged Chimeras / Anastasiya D. Blagoveshchenskaya ; Daniel F. Cutler4.：
Visualizing Protein Dynamics in Yeast with Green Fluorescent Protein / Christopher G. Burd5.：
Kinetic Analysis of Intracellular Trafficking in Single Living Cells with Vesicular Stomatitis Virus Protein G-Green Fluorescent Protein Hybrids / Koret Hirschberg ; Robert D. Phair ; Jennifer Lippincott-Schwartz6.：
Dual Color Detection of Cyan and Yellow Derivatives of Green Fluorescent Protein Using Conventional Fluorescence Microscopy and 35-mm Photography / Gisele Green ; Steven R. Kain ; Brigitte Angres7.：
Invertase Fusion Proteins for Analysis of Protein Trafficking in Yeast / Tamara Darsow ; Greg Odorizzi ; Scott D. Emr8.：
Introduction of Kex2 Cleavage Sites in Fusion Proteins for Monitoring Localization and Transport in Yeast Secretory Pathway / B. Diane Hopkins ; Ken Sato ; Akihiko Nakuno ; Todd R. Graham9.：
Lineage Analysis with Retroviral Vectors / Constance L. Cepko ; Elizabeth Ryder ; Christopher Austin ; Jeffrey Golden ; Shawn Fields-Berry ; John Lin10.：
Use of Pseudotyped Retroviruses in Zebrafish as Genetic Tags / Shawn Burgess ; Nancy Hopkins11.：
Tools for Analysis of Membrane Proteins / Section III.：
A Gene Fusion Method for Assaying Interactions of Protein Transmembrane Segments in Vivo / Jennifer A. Leeds ; Jon Beckwith12.：
Using SUC2-HIS4C Reporter Domain to Study Topology of Membrane Proteins in Saccharomyces cerevisiae / Christian Sengstag13.：
Detecting Interactions between Membrane Proteins in Vivo Using Chimeras / Igor Stagljar ; Stephan te Heesen14.：
Alkaline Phosphatase Fusion Proteins for Molecular Characterization and Cloning of Receptors and Their Ligands / Hwai-Jong Cheng15.：
Surface Chimeric Receptors as Tools in Study of Lymphocyte Activation / Bryan A. Irving ; Arthur Weiss16.：
Use of Chimeric Receptor Molecules to Dissect Signal Transduction Mechanisms / Warren J. Leonard17.：
Fusion Protein Toxins Based on Diphtheria Toxin: Selective Targeting of Growth Factor Receptors of Eukaryotic Cells / Johanna C. vanderSpek ; John R. Murphy18.：
Green Fluorescent Protein-Based Sensors for Detecting Signal Transduction and Monitoring Ion Channel Function / Micah S. Siegel ; Ehud Y. Isacoff19.：
Metabolic Labeling of Glycoproteins with Chemical Tags through Unnatural Sialic Acid Biosynthesis / Christina L. Jacobs ; Kevin J. Yarema ; Lara K. Mahal ; David A. Nauman ; Neil W. Charters ; Carolyn R. Bertozzi20.：
Signals for Addressing Proteins to Specific Subcellular Compartments / Section IV.：
Using Sorting Signals to Retain Proteins in Endoplasmic Reticulum / Hugh R. B. Pelham21.：
Directing Proteins to Nucleus by Fusion to Nuclear Localization Signal Tags / Heike Krebber ; Pamela A. Silver22.：
Identification, Analysis, and Use of Nuclear Export Signals in Saccharomyces cerevisiae / Karsten Weis23.：
Directing Proteins to Mitochondria by Fusion to Mitochondrial Targeting Signals / Kostas Tokatlidis24.：
Targeting Proteins to Plasma Membrane and Membrane Microdomains by N-Terminal Myristoylation and Palmitoylation / Wouter van't Hof ; Marilyn D. Resh25.：
Analysis of Function and Regulation of Proteins That Mediate Signal Transduction by Use of Lipid-Modified Plasma Membrane-Targeting Sequences / Gary W. Reuther ; Janice E. Buss ; Lawrence A. Quilliam ; Geoffrey J. Clark ; Channing J. Der26.：
Glycerolphosphoinositide Anchors for Membrane-Tethering Proteins / John D. Fayen ; Mark L. Tykocinski ; M. Edward Medof27.：
Fusions to Members of Fibroblast Growth Factor Gene Family to Study Nuclear Translocation and Nonclassic Exocytosis / I. Prudovsky ; M. Landriscina ; R. Soldi ; S. Bellum ; D. Small ; V. Andreeva ; Thomas Maciag28.：
Application of Chimeras in Monitoring and Manipulating Cell Physiology / Section V.：
Posttranslational Regulation of Proteins by Fusions to Steroid-Binding Domains / Didier Picard29.：
Tet Repressor-Based System for Regulated Gene Expression in Eukaryotic Cells: Principles and Advances / Udo Baron ; Hermann Bujard30.：
Coumermycin-Induced Dimerization of GyrB-Containing Fusion Proteins / Michael A. Farrar ; Steven H. Olson ; Roger M. Perlmutter31.：
Use of Glutathione S-Transferase and Break Point Cluster Region Protein as Artificia Dimerization Domains to Activate Tyrosine Kinases / Yoshiro Maru32.：
Recombinant Aequorin as Tool for Monitoring Calcium Concentration in Subcellular Compartments / Valerie Robert ; Paolo Pinton ; Valeria Tosello ; Rosario Rizzuto ; Tullio Pozzan33.：
Recombinant Aequorin as Reporter of Changes in Intracellular Calcium in Mammalian Cells / Jenny Stables ; Larry C. Mattheakis ; Ray Chang ; Stephen Rees34.：
Monitoring Protein Conformations and Interactions by Fluorescence Resonance Energy Transfer between Mutants of Green Fluorescent Protein / Atsushi Miyawaki ; Roger Y. Tsien35.：
Studies of Signal Transduction Events Using Chimeras to Green Fluorescent Protein / Tobias Meyer ; Elena Oancea36.：
Use of Fusions to Green Fluorescent Protein in the Detection of Apoptosis / Eve Shinbrot ; Collin Spencer ; Valerie Natale37.：
Synapto-pHluorins: Chimeras between pH-Sensitive Mutants of Green Fluorescent Protein and Synaptic Vesicle Membrane Proteins as Reporters of Neurotransmitter Release / Rafael Yuste ; Rebecca B. Miller ; Knut Holthoff ; Shifang Zhang ; Gero Miesenbock38.：
Studying Organelle Physiology with Fusion Protein-Targeted Avidin and Fluorescent Biotin Conjugates / Minnie M. Wu ; Juan Llopis ; Stephen R. Adams ; J. Michael McCaffery ; Ken Teter ; Markku S. Kulomaa ; Terry E. Machen ; Hsiao-Ping H. Moore39.：
Fluorescent Labeling of Recombinant Proteins in Living Cells with FlAsH / B. Albert Griffin ; Jay Jones40.：
Ubiquitin Fusion Technique and Its Descendants / Alexander Varshavsky41.：
Use of Phosphorylation Site Tags in Proteins / Sidney Pestka ; Lei Lin ; Wei Wu ; Lara Izotova42.：
John N.; Simon, Melvin I.; Balch, W. E.; Der, Channing J.; Hall, Alan Abelson, W. E. Balch, Channing J. Der, Alan Hall, Michael I. Johnson, Masayasu N. Kojima, Melvin I. Simon
ＥＢ
