| Introduction / Allen I. Laskin |
| The Enzyme Engineering Award |
| Active Site Flexibility in Enzyme Catalysis / Chen-Lu Tsou |
| Protein Disulfide Isomerase Assists Protein Folding as Both an Isomerase and a Chaperone / Chih-Chen Wang |
| A Toolbox of Recombinant Lipases for Industrial Applications / Claudia Schmidt-Dannert ; Jurgen Pleiss ; Rolf D. Schmid |
| Biosensors for Environmental Monitoring / Isao Karube ; Kazuyoshi Yano ; Satoshi Sasaki ; Yoko Nomura ; Kazunori Ikebukuro |
| New Recognition Elements in Biosensing / Frieder W. Scheller ; Frank Kleinjung ; Frank F. Bier ; Alexander Makower ; Barbara Neumann ; Ulla Wollenberger ; Iliya Kurochkin ; Arkadi Eremenko ; Anatoli Barmin ; Sven Klu[sz ligature]mann ; Jens-Peter Furste ; Volker A. Erdmann ; D. Mansuy |
| Neural Networks as "Software Sensors" in Enzyme Engineering / Susan Linko ; Yi-Hong Zhu ; Pekka Linko |
| Biosensors for Fermentation Process Control / Jianguo Liu ; Gaoxiang Li ; Shunzhen Xie ; Rushui Han ; Kemin Chen |
| Enzymatic Modification of Natural Compounds with Pharmacological Properties / Sergio Riva ; Danieli Monti ; Monica Luisetti ; Bruno Danieli |
| Novel Double Bond-Transferring Hydroxylation Reaction Involved in Microbial Metabolism of Eugenol / Hirotaka Furukawa ; Toru Nagasawa |
| Chiral Alcohol Synthesis with Microbial Carbonyl Reductases in a Water-Organic Solvent Two-Phase System / Sakayu Shimizu ; Michihiko Kataoka ; Keiko Kita |
| New Strategy of Thrombolysis: Conjunctive Effect of Plasminogen Activators with Different Pharmacokinetic Profile / Alexander V. Maksimenko |
| Production of "Neometalloenzymes" by De Novo Biosynthesis: New ELISA Method for Their Characterization / E. Nedonchelle ; C. Leduc ; X. Garcia ; R. Guzman ; M. A. Vijayalakshmi |
| Screening of Inhibitory Monoclonal Antibodies: A Critical Step for Producing Anti-idiotypic Catalytic Antibodies / Berangere Avalle ; Alain Friboulet ; Daniel Thomas |
| Properties of Artificial Proteins with Random Sequences / Tetsuya Yomo ; Irfan D. Prijambada ; Keizo Yamamoto ; Yasufumi Shima ; Seiji Negoro ; Itaru Urabe |
| A Selenium-Containing Abzyme, the Activity of Which Surpassed the Level of Native Glutathione Peroxidase / Guimin Luo ; Lan Ding ; Zhi Liu ; Tongshu Yang ; Jiazhan Ni |
| Directed Evolution of New Enzymes and Pathways for Environmental Biocatalysis / Lawrence P. Wackett |
| Fighting Nerve Agent Chemical Weapons with Enzyme Technology / Keith E. LeJeune ; Bryan C. Dravis ; Fangxiao Yang ; Amy D. Hetro ; Bhupendra P. Doctor ; Alan J. Russell |
| Multiple Intensified Performance of an Enzyme-Catalyzed Reaction in Organic Medium / Tsuneo Yamane ; Yugo Iwasaki ; Rosu Roxana ; Nobuyoshi Shimidzu ; Nobushige Doisaki |
| Coenzyme-Dependent Oxidoreductions in Organic Media / Patrick Adlercreutz ; Mats Andersson ; Hans Holmberg |
| Effect of Chemical Modification of Cyclodextrin Glycosyltransferase (CGTase) from Thermoanaerobacter sp. on Its Activity and Product Selectivity / Miguel Alcalde ; Francisco J. Plou ; Eitel Pastor ; Antonio Ballesteros |
| Intrinsic [alpha]-Chymotrypsin Activity Determination in Acetonitrile Containing Small Amounts of Aqueous Buffer / Raul J. Barros ; Andreas Paar ; Ernst Wehtje |
| Effect of Reductive Alkylation of Canadia rugosa Lipase on Its Enantioselective Esterification Reaction / M. Basri ; B. L. Th'ng ; C. N. A. Razak ; A. B. Salleh |
| Proteolysis of Aggregated Fibronectin: A Model for In Vivo Matrix Degradation / Hugues Berry ; Emmanuel Pauthe ; Olivier Gallet ; Veronique Larreta-Garde |
| Glucosylation by Dextranscrase: Modeling of Reaction Kinetics and Unconventional Products / K. Heincke ; B. Demuth ; H-J. Jordening ; K. Buchholz |
| High Selectivity of Reactions in Ice / B. Daum |
| Purification and Characterization of the Mutant Enzyme W117Y of the Dichloromethane Dehalogenase from Methylophilus sp. Strain DM11 / Baoli Cai ; Stephane Vuilleumier |
| Production, Properties, and Application of Xylanase from Aspergillus niger A3 / Jing-Min Cai ; Ke Wu ; Jie Zhang ; Ren-Rui Pan |
| Stability in Organic Solvent Mixtures of [beta]-Glucosidase and [beta]-Fructofuranosidase as Dry Powder and Entrapped in Poly-HEMA / Laura Cantarella ; Francesco Alfani ; Maria Cantarella |
| Characterization in UF-Membrane Reactors of Nitrile Hydratase from Brevibacterium imperialis CBS 489-74 Resting Cells / Agata Spera |
| Inactivation of Enzymes by Inert Gas Bubbling: A Kinetic Study / Mylene Caussette ; Alain Gaunand ; Henri Planche ; Pierre Monsan ; Brigitte Lindet |
| Cloning, Sequencing, and Expression in Escherichia coli of d-Hydantoinase Gene from Pseudomonas putida / Hwa-Yi Chen ; Hsin Tsai |
| Enzymatic Synthesis of Functional Materials / Songming Chen ; Ran Lu ; Xuezhong Zhang ; Xiangdong Chai ; Tiejin Li |
| Enzymatic Synthesis and Characterization of Co-oligo(8-hydroxyquinoline-p-phenylphenol) / Fengrong Sun |
| Horseradish Peroxidase-Catalyzed Preparation and Optoelectronic Property of Poly(1,5-dihydroxynaphthalene) Composite in Porous Silicon Nanohost / Peng Zhang ; Zhonghua Yang ; Dejun Wang |
| G-Type Nerve Agent Decontamination by Alteromonas Prolidase / Tu-chen Cheng ; Vipin K. Rastogi ; Joseph J. DeFrank ; Gamil P. Sawiris |
| An Immobilized Cell System for the Continuous Production of Lactic Acid from Molasses / Donal F. Day ; N. Thongwai |
| Enzyme-Catalyzed Synthesis of Polyester: Effect of the Microenvironment of Enzyme on Polyesterification / Huan Dong ; Zhi Wang ; Zheng-qiang Li ; De-lin You ; Si-ping Han ; Shu-gui Cao ; Jia-cong Shen |
| Lipase-Catalyzed Synthesis of Monoglycerides by Hydrolysis of Soybean Oil in AOT/Isooctane Reversed Micelles / Hai-lin Fan ; Ying Chu ; Gui-xia Yang ; Wei Zhang ; Jing-lin Liu ; Zi-sheng Wu |
| Study of Abzymes with Cytochrome P-450 Catalytic Activity / Ximeng Liu ; Sigeng Chen ; Yan Feng ; Gui Gao |
| Growth-Stimulating Effect of Shochu Wastewater on Lactic Acid Bacteria and Bifidobacteria / Yoshifumi Furuta ; Hideharu Takashita ; Toshiro Omori ; Kenji Sonomoto ; Ayaaki Ishizaki ; Masahiko Shimoda ; Hisatsugu Wada |
| Kinetic Studies of Abzyme with Glutathione Peroxidase Activity / Shu-juan Gao ; Mo Chen ; Wei Lin ; Gui-min Luo |
| Antioxidant Effects of Superoxide Dismutase and Horseradish Peroxidase on Lipid Peroxidation / Guang-cheng Zhao ; Tong-shu Yang |
| Production and Purification of Bacillus subtilis C4 Levansucrase: Kinetic Characterization of the Enzyme / Olivier Euzenat ; Alain Guibert ; Didier Combes |
| A Convenient Enzymatic Procedure for the Production of Aldose-Free d-Tagatose / Dietmar Haltrich ; Christian Leitner ; Wilfried Neuhauser ; Bernd Nidetzky ; Klaus D. Kulbe ; Jindrich Volc |
| Extracellular Proteinases from Extremophiles / Yasutaka Morita ; Quamrul Hasan ; Toshifumi Sakaguchi ; Yuji Murakami ; Kenji Yokoyama ; Eiichi Tamiya |
| Application of Thick Film Technology in Mass Production of Biosensors / Jamie J. Hu |
| cDNA Cloning and Heterologous Expression of Mouse CYP2G1 / Zi-Chun Hua ; Xinxin Ding |
| Low-Calorie Structured Lipid Synthesis by Lipase-Catalyzed Transesterification / Young Jang ; Jeong Jun Han ; Seok Joon Kwon ; Joon Shick Rhee |
| Novel Threonine Aldolases and Their Application to Stereospecific Synthesis of [beta]-Hydroxy-[alpha]-amino Acids / Masaru Wada ; Masahisa Ikemi ; Tadashi Morikawa ; Teruzo Miyoshi |
| Catalytic Properties of Lipase Entrapped in Alkyl-Substituted Organic Silicates / Koei Kawakami ; Shin-ya Furukawa |
| Reducing Side Reactions in Protease-Catalyzed Tripeptide (RGD) Synthesis / Jin-Eon So ; Bong-Kee Bae ; Byung-Gee Kim |
| Engineering the Thermostable d-Hydantoinases from Two Thermophilic Bacilli Based on Their Primary Structures / Geun-Joong Kim ; Joo-Ho Park ; Dong-Cheol Lee ; Hak-Sung Kim |
| Biochemical Properties of Thermostable D-Hydantoinase from Bacillus thermocatenulatus GH-2 / Seung-Goo Lee |
| Lipase-Catalyzed Synthesis of Lysophosphatidylcholine / Juhan Kim |
| Novel Bacteriocin of Pediococcus sp. ISK-1 Isolated from Well-Aged Bed of Fermented Rice Bran / Hirokazu Kimura ; Toshihiro Sashihara ; Hiromi Matsusaki |
| Phospholipase A from Cytosolic Fraction of Pyrococcus horikoshii Having High Reaction Temperature / Yoshitsugu Kosugi ; Yong-Goe Joh ; Anisur Rahman Khan ; Katsuhiko Higuchi ; Kazuhiko Ishikawa ; Ikuo Matsui |
| Enzymatic Synthesis of Saccharide Fatty Acid Esters / Xiufen Kou ; Jiali Xu |
| Activity and Stability of Proteases under High Pressure: Metalloprotease and Serine Carboxypeptidase / Shigeru Kunugi ; Naoki Tanaka |
| Converting T4 Phage Lysozyme into a Transglycosidase / Ryota Kuroki ; Keiichi Morimoto ; Brian W. Matthews |
| A Mechanistic and Kinetic Approach to the Regulation by Oxygen of Soybean Lipoxygenase-1 Catalysis / Helene Debat |
| A Cultivation Strategy of Recombinant Escherichia coli for Mass Production of Thermostable D-Hydantoinase / Yoo-Kyong Cha ; Cheo-Young Lee |
| Development of Recombinant Bacteria for the Degradation of Dibenzothiophene / Ki Jun Jeong ; Hyun Sook Lee ; Sang Yup Lee ; Yong Keun Chang |
| Conformation Changes of Alcohol Dehydrogenase from Baker's Yeast (YADH) Induced by Hydrostatic Pressure / Sandrine Dallet ; Patrick Choisy ; Marie-Dominique Legoy |
| Purification and Properties of 3[alpha]-Hydroxysteroid Dehydrogenase and 3-Keto-5[beta]-steroid-[Delta][superscript 4]-dehydrogenase / XiaoLin Gao ; JianGuo Liu ; GaoXiang Li |
| Assembly and Folding of Glucose Oxidase at the Water-Air Surface / Guoliang Dai ; Jinru Li ; Long Jiang |
| Lipase Biocatalysis in the Production of Aromatic Polyesters / Xiao Yan Wu ; Yu-Yen Linko ; Jukka Seppala |
| Enantioselective Esterification of Racemic Acid Catalyzed by Lipase in a Mixed Solvent System / Jian-He Xu ; Jun-Min Liu ; Xue-Shu Xu ; Qin-Sheng Yuan |
| Thrombolytic Effect after Sequential Double-Bolus Administration of Tissue Plasminogen Activator and Urokinase-Fibrinogen Conjugate into Dogs with Venous Thrombosis / Elena G. Tischenko ; Vladimir L. Golubykh |
| Enzymatic Synthesis of Edible Surfactants from Food Ingredients / Ryuichi Matsuno ; Shuji Adachi |
| Purification, Identification, and Effective Production of a Peptide Antibiotic Produced by Lactococcus lactis IO-1 (JCM 7638) / Noppawan Chinachoti |
| A Thermophilic Alkaline Xylanase Produced by Thermophilic Alkalophilic Bacillus sp. Strain NM-118 / N. S. Mendoza ; M. B. Bigol ; N. M. Unciano ; B. F. Almonte ; C. C. Borromeo |
| Improvement in the Organic Solvent Stability of Pseudomonas Lipase by Random Mutation / Hideo Nakano ; Yoshiro Ide ; Toshihiko Tsuda ; Junhao Yang ; Shouji Ishii |
| Engineering of Enzyme Monolayer for Industrial Biocatalysis: An Overview / Claudio Nicolini |
| Strategies to an Efficient Enzymatic Production of Xylitol / Peter Mayr |
| Process Stability of Glucose-Fructose Oxidoreductase from Zymomonas mobilis: Role of Reactive Thiols Probed by Chemical Modification / Monika Furlinger ; Dorothee Gollhofer ; Markus Satory |
| Enzymatic Synthesis of Mannitol: Reaction Engineering for a Recombinant Mannitol Dehydrogenase / Matthias Slatner ; Gudrun Nagl |
| Sorbitol Oxidase from Microorganisms / Koehi Oda ; Kazumi Hiraga |
| Enzymatic and Structural Approaches of the Thermolysin Mechanism in Glycerol-Containing Media / Manuel Dauchez ; Maurice Berjot ; Jean-Pierre Monti ; Alain J. P. Alix |
| Optimization of Process Conditions for Gamanase-Coconut Oil Extraction / Chay B. Pham ; Merlita T. Punzal ; Ariene R. Moreno ; Lilia M. Soriano ; William G. Padolina |
| Biomodification of Selected Tropical Oils for the Production of Specialty Fats and Oils / Laura J. Pham ; Myla A. Gregorio ; Edmar P. Casa |
| Enzymatic Modification of Straw Pulp and Characterization of Alkaline Xylanases from Pseudomonas sp / Ruitian Liu ; Yinbo Qu ; Bomin Wang ; Jiachuan Zhen ; Peiji Gao ; Yinghui Jiang ; Guihua Yang ; Zhaocheng Li |
| Development of an ELISA for Detection of Parathion, Carbofuran, and 2,4-Dichlorophenoxyacetic Acid in Water, Soil, Vegetables, and Fruits / F. Salam ; K. Ampon |
| Purification of a Fusion Protein Using the Family VI Cellulose-Binding Domain of Clostridium stercorarium XynA / Kazuo Sakka ; Shuichi Karita ; Tetsuya Kimura ; Kunio Ohmiya |
| Water Activity Effect on Lipase Immobilized onto Different Compositions of Poly(HEMA-MMA) / N. M. Esa ; W. M. Z. Yunus ; M. B. Ahmad |
| Use of a Laccase-Column for Flow-Injection Calorimetry / Ikuo Satoh ; Ikuko Sakurai |
| Enhancement Effect of Reverse Micelles on Enzymatic Synthesis of Cephalexin / Yi-Feng Shi ; Xing-Ju Yu ; Quan Yuan |
| Growth-Stimulation of Bifidobacterium by Natural Rubber Serum Powder / Shin-Ichi Etoh ; Hirokazu Oiki |
| Levansucrase of Rahnella aquatilis ATCC33071: Gene Cloning, Expression, and Levan Formation / Ki-Bang Song ; Jeong-Woo Seo ; Min-Gon Kim ; Sang-Ki Rhee |
| Upward Shift of Thermotolerance of Cold Water Fish and Mammalian Trypsins upon Chemical Modification / R. Venkatesh ; P. V. Sundaram |
| Improved Stability of Soybean and Horsearadish Peroxidases by Covalent Chemical Modification |
| Catalytic Efficiency of Covalently Modified Proteases against Proteinaceous Substrates / S. Deepthi |
| Totally Enzymatic Synthesis of Peptides: Penicillin Acylase-Catalyzed Protection and Deprotection of Amino Groups as Important Building Blocks of This Strategy / Vytas K. Svedas ; Alexander I. Beltser |
| Molecular Breeding of Polysaccharide-Utilizing Yeast Cells by Cell Surface Engineering / Mitsuyoshi Ueda ; Toshiyuki Murai ; Yumi Shibasaki ; Naomi Kamasawa ; Masako Osumi ; Atsuo Tanaka |
| Enhancement of Glucose Biosensor Response Ability by Addition of Hydrophobic Gold Nanoparticles / Fangqiong Tang |
| Micromachined Multifunctional Biosensor Array |
| Synthesis of Adenosine 5'-Triphosphate Derivatives and Their Substrate Activities to Thermostable Glucokinases / Kosuke Tomita ; Takashi Ikeda ; Toshihiro Takahashi |
| Enantioselective Esterification of Racemic Naproxen Catalyzed by Immobilized Lipase in a Microaqueous System / Dongzhi Wei ; Yumin Cui ; Qin Yu |
| High-Affinity Peptide Ligands for Pancreatic [alpha]-Amylase by Phage Display / D. W. S. Wong ; G. H. Robertson |
| Dimer Magic / Shan Wu ; Grant Ganshaw ; Constant Hsia ; Chung Liu ; A. J. Poulose ; Richard Bott |
| Improving the Catalytic Activity of Phenylalanine Aminotransferase of Escherichia coli by Site-Specific Mutation / Szu-Pei Wu ; Chichi Liu ; Tzann-Shun Hwang ; Jin-Tann Chen |
| Effect of Exogenous Electron Donors and Water-Soluble Polymers on the Propene-Epoxidizing Activity of Methylomonas Z201 Cells / Shiwen Xia ; Yaoting Yu ; Li Yuchi ; Runnan Shen ; Shuben Li |
| Effect of Water Content on Enzyme Activity and Enantioselectivity of Lipase-Catalyzed Esterification of Racemic Ibuprofen in Organic Solvents / Yu-Chun Xie ; Hui-Zhou Liu ; Jia-Yong Chen |
| Construction of Urea-Resistant Staphylococcus aureus V8 Protease Derivatives and Application in the Releasing of Peptide Hormones from Fusion Proteins / Masayuki Yabuta ; Kazuhiro Ohsuye |
| Screening of the Fungus Whose Multienzyme System Has Catalytic Detoxification Activity towards Aflatoxin B[subscript 1] (Part I) / Dong-Sheng Yao ; Ren Liang ; Da-Ling Liu ; Lian-Quan Gu ; Lin Ma ; Wei-Qiang Chen |
| Armillariella tabescen Enzymatic Detoxification of Aflatoxin B[subscript 1] (Part II) |
| Armillariella tabescen Enzymatic Detoxification of Aflatoxin B[subscript 1]: Part III. Immobilized Enzymatic Detoxification |
| Analysis of Glycolysis Relevant Compounds in Saliva by Microbiosensors / Kentaro Yoda ; Kouji Shimazaki ; Yuichiro Ueda |
| Enzymatic Synthesis of Phenol-Sucrose Copolymer Using Peroxidase in Organic Solvent and Its Reaction Optimization / Hyun Joo ; Young Je Yoo |
| Methanol Biosynthesis by Methanotrophic Bacterial Cells--Effects of Various Immobilization Methods on Biocatalytic Activity of Immobilized Cells / Chili Yu ; Chungu Xia |
| Extraction and Some Properties of Soluble Methane Monooxygenase of Methylosinus trichosporium IMV 3011 |
| Biochemical Characterization of [alpha]-Aspartyl Dipeptidase: Cloning and Expression of Its Gene / Hongying Zhang ; Jin Zhang ; Xiaoping Wang ; Wei Yang ; Jun Lu |
| Studies on the Amino Acid Residues of the Active Site of [alpha]-Aspartyl Dipeptidase / Shui Hao |
| Studies on the Properties of Mutants of Aspartase from Escherichia coli W |
| The Design and Construction of a New Nucleozyme |
| Bioactive Small Peptides from Soybean Protein / Xue-Zhong Zhang ; Huan-Yu Wang ; Xue-Qi Fu ; Xiao-Xia Wu ; Guang-Li Xu |
| Lipase-Catalyzed Enantioselective Transesterification of Cyanohydrins for the Synthesis of (S)-[alpha]-Cyano-3-phenoxybenzyl Acetate / Ying Zhu ; Li-rong Yang ; Zi-qiang Zhu ; Shanjing Yao ; Peilin Cen |
| Kinetics of Butyl Acetate Synthesis by Lipase-Catalyzed Transesterification in Hexane / Huijuan Xu |
| Effects of Organic Solvent and Acylating Agent on Lipase-Catalyzed Esterification of a Chiral Chlorohydrin in Nonaqueous Media / Ying-Ping Zhuang ; Si-Liang Zhang |
| Index of Contributors |
| Introduction / Allen I. Laskin |
| The Enzyme Engineering Award |
| Active Site Flexibility in Enzyme Catalysis / Chen-Lu Tsou |
| Protein Disulfide Isomerase Assists Protein Folding as Both an Isomerase and a Chaperone / Chih-Chen Wang |
| A Toolbox of Recombinant Lipases for Industrial Applications / Claudia Schmidt-Dannert ; Jurgen Pleiss ; Rolf D. Schmid |
| Biosensors for Environmental Monitoring / Isao Karube ; Kazuyoshi Yano ; Satoshi Sasaki ; Yoko Nomura ; Kazunori Ikebukuro |
