The Phage Nanoparticle Toolkit / George P. SmithChapter 1: |
Introduction / 1.1: |
Virion Structure and Purification / 1.2: |
Intrusion / 1.3: |
DNA Replication Cycle and Gene Expression / 1.4: |
Extrusion of Progeny Virions / 1.5: |
Display of Guest Peptides / 1.6: |
The Engineer's Toolkit / 1.7: |
Acknowledgements |
References |
The Roles of Structure, Dynamics and Assembly in the Display of Peptides on Filamentous Bacteriophage / Stanley J. OpellaChapter 2: |
Molecular and Structural Biology of Filamentous Bacteriophage / 2.1: |
Packaging of the Genome into Filamentous Bacteriophage / 2.2: |
Structural Form of the Major Coat Protein / 2.3: |
Membrane-bound Form of Filamentous Bacteriophage Coat Proteins / 2.4: |
Assembly / 2.5: |
Phage Display / 2.6: |
Conclusion / 2.7: |
Quantitative Analysis of Peptide Libraries / Lee MakowskiChapter 3: |
Assessing the Quality of a Phage-displayed Library / 3.1: |
Peptide Sequence Censorship / 3.2.1: |
Experimental Measures / 3.2.2: |
Conceptual Measures / 3.2.3: |
Quantitative Measures / 3.2.4: |
Assessing the Quality of an Affinity Screen Experiment / 3.3: |
Change in Diversity / 3.3.1: |
Change in Information / 3.3.2: |
Identification of Motifs in a Peptide Population / 3.4: |
Similarity Matrices / 3.5: |
Identification of Binding Sites in Proteins / 3.6: |
Identification of Binding Proteins in a Proteome / 3.7: |
Relic / 3.8: |
Discussion |
Phage-mediated Drug Delivery / Valery A. Petrenko ; Prashanth K. JayannaChapter 4: |
Targeting of Drugs/Drug Carrier Systems / 4.1: |
Targeting Ligands / 4.3: |
Phage-displayed Libraries as a Source of Peptide Targeting Ligands / 4.4: |
Bacteriophage Capsid-mediated Drug Delivery / 4.5: |
Drug-bearing Filamentous Phage as Targeted Chemotherapeutics / 4.6: |
Phage Fusion Proteins as Targeting Ligands for Nanomedicines / 4.7: |
Imaging with Bacteriophage-derived Probes / Susan L. Deutscher ; Kimberly A. Kelly4.8: |
Selection of Bacteriophage as Imaging Probes / 5.1: |
Imaging Agents / 5.1.1: |
Phage Nanoparticles / 5.1.2: |
Phage Display for Imaging Probe Discovery / 5.1.3: |
Radiolabled Phage as Imaging Agents / 5.2: |
Optical Molecular Imaging with Phage / 5.3: |
Phage-based Pathogen Biosensors / Suiqiong Li ; Ramji S. Lakshmanan ; Bryan A. Chin5.4: |
Threat of Pathogenic Microorganisms / 6.1: |
Pathogen Detection Techniques / 6.1.2: |
Current Trends and Existing Methodologies for Pathogen Detection / 6.2: |
Conventional Pathogen Detection Techniques / 6.2.1: |
Polymerase Chain Reaction (PCR) / 6.2.2: |
Enzyme-linked Immunosorbent Assay (ELISA) / 6.2.3: |
Biosensor Techniques / 6.2.4: |
Biomolecular Recognition Element / 6.2.5: |
Whole Filamentous Bacteriophage Particles as a Biorecognition Probe / 6.3: |
Phage Immobilization on Biosensor Platforms / 6.3.1: |
Current Trends in Development of Phage-based Biosensors / 6.3.2: |
Phage-based Magnetoelastic Particle Resonator Biosensors / 6.4: |
Magnetoelastic (ME) Particle Resonator Sensor Platform / 6.4.1: |
Fabrication of the Sensor Platform / 6.4.2: |
ME Biosensor Assembly / 6.4.3: |
Performance of Phage-based ME Biosensors / 6.4.4: |
Phage-mediated Detection of Biological Threats / Steven Ripp6.5: |
Phage Typing Schemes / 7.1: |
Exploiting Phage Specificity for Bacterial Detection / 7.3: |
Labeled Phage / 7.3.1: |
Reprter Phage / 7.3.2: |
Phage Amplification / 7.3.3: |
Electrochemical-based Sensing Assays / 7.3.4: |
Surface Plasmon Resonance-based Sensing Assays / 7.3.5: |
The Phage-mediated Adenylate Kinase Assay / 7.3.6: |
Genetically Engineered Virulent Phage Banks for the Detection and Control of Bacterial Biosecurity Threats / Francois Iris ; Flqvie Pouillot ; Helene Blois ; Manuel Geo ; Paul-Henri Lampe7.4: |
Host Range engineering / 8.1: |
Production of a Genetically Engineered T4 Phage Bank with Vastly Increased Host Range / 8.3: |
Reversible Inhibition of the T4 Lytic Cycle Within the Bacterial Host / 8.4: |
Large-scale Recombinations into the Genomes of an Infective Wild-type T4 Population / 8.5: |
Construction of a T4 Bank of Host Range Variants / 8.6: |
Conclusion and Perspectives / 8.7: |
Methods / 8.9: |
High-fidelity PCR / 8.9.1: |
Error-prone PCR / 8.9.2: |
Selective High-fidelity Amplification of Desired Fragments / 8.9.3: |
Reconstruction of Sequence Through PCR / 8.9.4: |
DNA Sequencing and Analysis / 8.9.5: |
Production and Expression of Non-functional E. coli Rho Genes / 8.9.6: |
Construction and Expression of the Heat-inducible Red-Recombinase System / 8.9.7: |
Site-directed Chemical Modification of Phage Particles / Lana Saleh ; Christopher J. NorenChapter 9: |
Unique Chemical Properties of Selenocysteine Compared with Cysteine / 9.1: |
In vivo Incorporation of Sec by E. coli / 9.3: |
Construction of Selenopeptide-displayed Phage Libraries / 9.4: |
Applications Using Selenopeptide Phage Display / 9.5: |
Screening for Sec Insertion in vivo: Investigating the Stringency of E. coli SECIS Requirements Using Phage Display / 9.5.1: |
Catalysis-based Selection of Novel Enzyme Activities from Substrate-appended Phage Libraries / 9.5.2: |
Mechanical Manipulation of M13 Phage / 9.5.3: |
Filamentous Fhage-templated Synthesis and Assembly of Inorganic Nanomaterials / Binrui Cao ; Chuanbin Mao9.6: |
Virion Structure and Phage Display / 10.1: |
Biology / 10.2.1: |
Chemistry / 10.2.2: |
Site-specific Engineering of the Virion Surface / 10.2.3: |
Liquid Crystalline Behavior / 10.2.4: |
Exploiting Phage Display to Alter Surface Chemistry by Selection Rather Than Rational Design225 / 10.3: |
Random Peptide Libraries225 / 10.3.1: |
Affinity Selection ('Biopanning')226 / 10.3.2: |
Synthesis and Assembly of Inorganic Materials on Individual Virions / 10.4: |
Synthesis and Assembly of Inorganic Materials on a Self-assembled Phage Scaffold / 10.5: |
Applications of Phage-templated Nanomaterials / 10.6: |
Summary and Outlook / 10.7: |
Phage Vaccines and Phage Therapy / Karen ManoutcharianChapter 11: |
Introduction to Phage / 11.1: |
Phage Immunogens / 11.2: |
Epitope Discovery with Phage Libraries and Phage Vaccines / 11.3: |
Autoimmune Disorders / 11.4: |
Cancer / 11.5: |
Neurological Disorders / 11.6: |
Other Diseases / 11.7: |
Antibacterial Therapy / 11.8: |
Subject Index |
The Phage Nanoparticle Toolkit / George P. SmithChapter 1: |
Introduction / 1.1: |
Virion Structure and Purification / 1.2: |